The effects of CYPIA1 gene polymorphism and p16 gene methylation on the risk of lung cancer in a Chinese population

Objective： To investigate the relationship between the genetic polymorphism of CYP1A1 and the genetic susceptibility to lung cancer as well as to study the effects of the methyiation in p16 gene on the risk of lung cancer in a Chinese population. Methods： A case control study was conducted among 47 cases of lung cancer and 94 controls. The genetic polymorphism of CYP1A1 was tested with method of PCR-RFLP, and a methylation-specific PCR （MSP） was performed to detect p16 methylation. Results： It showed that there was no significant difference in frequencies of the genotypes of CYP1A1 between the two groups （P 〉 0.05）. Synergistic effects were not found between smoking and CYP1AI. Methylated p16 gene was found in 44.7% （21/47） of lung cancer tissues and in 17.0% （8/47） of normal lung tissues with significant difference （P 〈 0.05）. Conclusion： The genetic polymorphism of CYP1A1 does not increase the risk of lung cancer in a Chinese population. The methylation in p16 gene may be the most common mechanism to inactivate p16 gene in lung cancer, and is not significantly associated with genotype of CYP1A1,     

CYP1A1、GSTM1基因多态性与肺癌易感性的研究

目的:探讨CYP1A1、GSTM1基因多态性与肺癌易感性之间的相关性。方法:利用RFLP-PCR(限制性片段长度多态性-聚合酶链反应)方法检测65例原发性肺癌和60例非肿瘤患者CYP1A1、GSTM1基因,再用NcoI及HinfI两种内切酶识别CYP1A1等位基因亚型。结果:1)肺癌组与对照组CYP1A1等位基因型Ile/Ile、Ile/Val、Val/Val的频率总体分布无显著性差异;但肺癌组CYP1A1(Val/Val)基因型频率(18.5%)明显高于对照组(8.3%),两组差异有显著性(P<0.05)。2)肺癌组GSTM1(-)基因型的频率(63.1%)明显高于对照组(45.0%),P<0.05。3)两种等位基因联合分析发现,与携带CYP1A1(Ile/Ile)/GSTM1(+)基因型的个体相比:CYP1A1(Ile/Ile)/GSTM1(-)以及CYP1A1(Ile/Val+Val/Val)/GSTM1(+)基因型个体患肺癌的风险度较高,OR分别为3.82(95.0%CI,1.27～11.45)和3.5(95.0%CI,1.18～10.41);而CYP1A1(Val/Val)/GSTM1(-)基因型个体患肺癌的风险度最高,OR为10.5(95.0%CI,1.70～64.73)。4)进一步分层分析发现,CYP1A1(Ile/Val+Val/Val)等位基因型主要增加鳞癌的危险性;而GSTM1基因型组织类型无明显的相关性。5)在分析吸烟对肺癌易感性的影响时发现,CYP1A1(Ile/Val+Val/Val)及GSTM1(-)等位基因型与吸烟有协同作用,并与至发病时的累积吸烟量有关。结论:CYP1A1(Val/Val     

CYP1A1基因多态性与p16基因甲基化对中国人肺癌发病的影响

Objective:To investigate the relationship between the genetic polymorphism of CYP1A1 and the genetic susceptibility to lung cancer as well as to study the effects of the methylation in p16 gene on the risk of lung cancer in a Chinese population.Methods:A case control study was conducted among 47 cases of lung cancer and 94 controls.The genetic polymorphism of CYP1A1 was tested with method of PCR-RFLP,and a methylation-specific PCR(MSP)was performed to detect p16 methylation.Results:It showed that there was no significant difference in frequencies of the genotypes of CYP1A1 between the two groups(P>0.05).Synergistic effects were not found between smoking and CYP1A1.Methylated p16 gene was found in 44.7%(21/47)of lung cancer tissues and in 17.0%(8/47)of normal lung tissues with significant difference(P< 0.05).Conclusion:The genetic polymorphism of CYP1A1 does not increase the risk of lung cancer in a Chinese population. The methylation in p16 gene may be the most common mechanism to inactivate p16 gene in lung cancer,and is not significantly associated with genotype of CYP1A1.     

DNA repair gene polymorphisms in the nucleotide excision repair pathway and lung cancer risk: A meta-analysis

Objective: A number of studies have reported the association of "XPA", "XPC", "XPD/ERCC2" gene polymorphisms with lung cancer risk. However, the results were conflict. To clarify the impact of polymorphisms of "XPA", "XPC", "XPD/ERCC2", on lung cancer risk, a meta-analysis was performed in this study. Methods: The electronic databases PubMed and Embase were retrieved for studies included in this meta-analysis by "XPA", "XPC", "XPD/ERCC2", "lung", "cancer/neoplasm/tumor/carcinoma", "polymorphism" (An upper date limit of October, 31, 2009). A meta-analysis was performed to evaluate the relationship among XPA, XPC and XPD polymorphism and lung cancer risks. Results: A total of 31 publications retrieved from Pubmed and Embase included in this study. XPC A939C CC genotype increased lung cancer risk in total population (recessive genetic model: OR=1.23, 95% CI:1.05-1.44; homozygote comparison: OR=1.21,95%CI:1.02-1.43and CC vs. CA contrast: OR=1.25,95%CI:1.06-1.48), except in Asians. XPD A751C, 751C allele and CC genotype also increased lung cancer risk in total population and in Caucasians (recessive genetic model: Total population: OR=1.20, 95%CI:1.07-1.35). No significant correlation was found between XPD A751C and lung cancer risk in Asians and African Americans. XPD G312A AA genotype increased lung cancer risk in total population, in Asians and Caucasians(recessive genetic model: Total population: OR=1.20, 95%CI: 1.06-1.36). No significant association was found between XPA G23A, XPC C499T, XPD C156A and lung cancer risk. Conclusion: Our results suggest that the polymorphisms in XPC and XPD involve in lung cancer risks. XPA polymorphisms is less related to lung cancer risk.     

Relationship between ERCC2 Polymorphism and Risk of Lung Cancer in Chinese Nonsmoker

Objective: Excision repair cross-complimentary group 2 (ERCC2) is one of the important DNA repair genes. ERCC2 codon 751 polymorphism has been shown to modulate cancer risk. We therefore assessed the relationship between the ERCC2 polymorphism and the susceptibility to lung cancer in nonsmoking females via a hospital-based case-control study. Methods: There were 105 lung cancer cases and matched healthy controls in this study. Information concerning demographic and risk factors was obtained, each person donated 2 ml blood for biomarker testing. ERCC2 genotypes were determined by PCR-RFLP method. All of the statistical analyses were performed with SPSS (v 12.0). Results: All of the subjects in this study were nonsmoking females in Shenyang. There was significant difference between the frequencies of ERCC2 polymorphism in cancer cases and controls (P<0.05). The frequencies of ERCC2 751 Gln allele were 6.2% in controls and 13.8% in cancer cases. The individuals with Lys/Gln Gln/Gln combined genotype were at an increased risk for lung cancer as compared with those carrying the Lys/Lys genotype (adjusted OR=2.80, 95%=CI 1.21?6.48). We analyzed the environmental risk factors for lung cancer in nonsmoking females. The cancer patients showed a higher prevalence of exposure to cooking fumes compared with controls (OR=2.44, P<0.05). Furthermore, an interaction between exposure to cooking fumes and the variant ERCC2 751 Gln allele on the risk of lung cancer was observed. Individuals with both risk genotype and exposure to cooking fumes had a higher risk of cancer than those with only one of them. Conclusion: The above findings indicate that the genetic polymorphism in the ERCC2 codon 751 is associated with the risk of lung cancer in nonsmoking females.     

中国四川北部肺癌患者谷胱苷肽硫转移酶M1基因多态性分析

Objective To analyze the genetic polymorphism of GSTM1 to lung cancer patients in north Sichuan of China and compare with race from other district.Methods PCR-based technique was used to detect the genotypes of GSTM1 in lung cancer patients.Results In local lung cancer patients,the frequency of homozygous deletions(null genotype) for GSTM1 was 58.4 % (73/125).Among the patients,the frequencys of null genotype for GSTM1 were 62.5 % (20/32) in female,56.9 % (53/93) in male,56.1% (32/57)in patients with squamous cell carcinoma and 54.8 % (17/31) in patients with adenocarcinoma,respectively.The frequency of deletions of GSTM1 in lung cancer patients from north Sichuan of China is slightly exceeding those of Europe and Americas (P ＜0.05) and similar to the domestic result (P ＞0.05).Conclusion The genetic polymorphism of GSTM1 to lung cancer patients in north Sichuan of China dosen' t show distinguished feature for this district and race.     

中国四川北部肺癌患者谷胱苷肽硫转移酶M1基因多态性分析

Objective To analyze the genetic polymorphism of GSTM1 to lung cancer patients in north Sichuan of China and compare with race from other district.Methods PCR-based technique was used to detect the genotypes of GSTM1 in lung cancer patients.Results In local lung cancer patients,the frequency of homozygous deletions(null genotype) for GSTM1 was 58.4 % (73/125).Among the patients,the frequencys of null genotype for GSTM1 were 62.5 % (20/32) in female,56.9 % (53/93) in male,56.1% (32/57)in patients with squamous cell carcinoma and 54.8 % (17/31) in patients with adenocarcinoma,respectively.The frequency of deletions of GSTM1 in lung cancer patients from north Sichuan of China is slightly exceeding those of Europe and Americas (P ＜0.05) and similar to the domestic result (P ＞0.05).Conclusion The genetic polymorphism of GSTM1 to lung cancer patients in north Sichuan of China dosen' t show distinguished feature for this district and race.     

A systemic review of glutathione S-transferase P1 Ilel05Val polymorphism and colorectal cancer risk

Objectives： To investigate the correlation between glutathione S-transferase P1 （GSTP1） Ilel05Val polymorphism and colorectal cancer （CRC） risk. Methods： Studies were identified to investigate the association between GSTP1 Ilel05Val polymorphism and CRC risk. Systematic computerized searches of the PubMed, Chinese National Knowledge Infrastructure, WANFANG and SinoMed were performed. Summary odds ratios （OR） and 95% confidence intervals （95 % CI） were used to measure GSTP 1 Ile 105Val polymorphisms and CRC risk. Results： A total of 23 retrospective studies were included in the meta-analysis. During all studies including 6,981 cases and 8,977 controls, sample sizes ranged from 146 to 2,144. Overall, the pooled results revealed that lie 105Val polymorphism was not associated with CRC risk and confused results were found in subgroup analyses. Further meta-analyses were conducted after excluding low-quality studies. GSTP1 Ilel05Val is associated with increased risk of CRC limited in studies with matched control. There was no significant heterogeneity in all genetic comparisons, but heterogeneity existed in subgroup analyses of heterozygous and dominant comparisons. The meta-regression analyses indicated that matched controls were the significant factor influencing between-study heterogeneity in all possible influential factors including published year, ethnicity, source of control, sample size, Hardy-Weinberg equilibrium （HWE） in control and matched controls. Sensitivity analysis revealed the pooled ORs were not changed before and after removal of each single study in all genetic comparisons, indicating the robustness of the results. Conclusions： GSTP1 Ilel05Val might be associated with increased risk of CRC. However, more high- quality case-control studies should be performed to confirm the authenticity of our conclusion.     

Meta-Analysis of the Association between Mir-196a-2 Polymorphism and Cancer Susceptibility

Objective MicroRNA plays a vital role in gene expression,and microRNA dysregulation is involved in carcinogenesis.The miR- 196a-2 polymorphism rsll614913 is reportedly associated with cancer susceptibility.This meta-analysis was performed to assess the overall association of miR-196a-2 with cancer risk. Methods A total of 27 independent case-control studies involving 10,435 cases and 12,075 controls were analyzed for the rsll614913 polymorphism. Results A significant association was found between rsll614913 polymorphism and cancer risk in four genetic models(CT vs.TT, OR=1.15,95%CI=1.05-1.27;CC vs.TT,OR=1.23,95%CI=1.08-1.39;Dominant model,OR=1.17,95%CI=1.06-1.30;Additive model, OR=1.08,95%CI=1.01-1.14).In the subgroup analysis of different tumor types,the C allele was associated with increased risk of lung, breast,and colorectal cancer,but not with liver,gastric,or esophageal cancer.In the subgroup analysis by ethnicity,a significantly increased risk of cancer was found among Asians in all genetic models,but no associations were found in the Caucasian subgroup. Conclusions The meta-analysis demonstrated that the miR-196a-2 polymorphism is associated with cancer susceptibility,especially lung cancer,colorectal cancer,and breast cancer among Asian populations.     

Association of the p73 Gene G4C14-to-A4T14 Polymorphism with Increased Gastric Cancer Risk in a Northwestern Chinese Population

OBJECTIVE To evaluate the p73 gene G4C14-to-A4T14 double nucleotide polymorphism with both increased gastric cancer(GC) risk and different histological subtypes of GC in a northwestern Chinese population. METHODS Genotyping of the polymorphism of the p73 gene was conducted with PCR-CTPP. RESULTS All 385 GC patients including 305 diffuse-type and 80 intestinal-type cases and 412 healthy controls were investigated.The frequencies of p73 AT/AT,AT/GC,and GC/GC genotypes were 28.1%,47.1%,and 24.8% in the controls,and were 22.0%,45.0%,and 33.0% in GC cases respectively;the GC/GC homozygote frequency was higher in GC cases,mainly in diffuse type compared to the controls with OR=1.71(1.16～2.51) and 1.87 (95%CI,1.24～2.81) respectively.The results showed that carriers of the p73 G4A GC/GC homozygote had a 1.71-time higher risk of GC,especially of the diffuse-type GC compared to the controls. The carriers of the AT/GC heterozygote also had a slightly increased risk of GC cancer,mainly on intestinal-type GC.This is the first report that the p73 G4A double-nucleotide polymorphism is associated with an increased risk of diffuse-type gastric cancer. CONCLUTION The p73 G4A GC/GC genotype is associated with an increased risk of gastric cancer,especially of the GC diffuse-type.     

Effects of cancer on patients with COVID-19:a systematic review and meta-analysis of 63,019 participants

Objective:Patients with underlying diseases are more vulnerable to coronavirus disease 2019(COVID-19).The purpose of this study was to investigate cancer incidence in patients with COVID-19 and to determine whether cancer was associated with mortality among patients with COVID-19.Methods:Electronic searches of Pub Med,Embase,Cochrane,Web of Science,and med Rxiv were conducted to collect studies that provided data regarding the incidence and mortality of cancer patients with COVID-19.Meta-analyses were used to estimate pooled incidences,risk ratios(RRs),and 95%confidence intervals(CIs)using a random-effects model.Heterogeneity among studies was detected using I2 statistics.Results:A total of 19 retrospective studies involving 63,019 patients(2,682 patients with cancer)were included.Meta-analysis showed that the pooled incidence of cancer in COVID-19 patients was 6%(95%CI:3%–9%).The mortality rate of COVID-19 patients with cancer was higher than that of those without cancer[risk ratio(RR):1.8,95%CI:1.38–2.35,P<0.01].Studies on specific types of cancer showed that among COVID-19 patients,the mortality rate of lung cancer patients was higher than that of patients without lung cancer(RR:1.8,95%CI:0.85–3.80,P=0.02).Conclusions:Patients with cancer were more susceptible to COVID-19.As a risk factor,cancer increased mortality among COVID-19 patients.Among COVID-19 patients with cancer,those who had lung cancer had a higher mortality than those without lung cancer.Our findings suggested that clinicians should pay more attention to cancer patients diagnosed with COVID-19 and provide useful information for their clinical management.     

Differential Diagnoses of Solitary Pulmonary Nodules in Patients with an Extrathoracic Malignant Tumor:CT and Parthologic Correlations

OBJECTIVE To determine the possibility of definitive diagnosis for solitary pulmonary nodules in patients with a primary extrathoracic malignant neoplasm (ETM-SPN), and to further evaluate the value of CT for differential diagnosis in ETM-SPN by a multivariate retrospective study.METHODS Eighty-three patients with pathologically and clinically proven ETM-SPN with a diameter smaller than 3 cm were included in this study.The pathological characteristics of the SPN were correlated with those of the extrathoracic neoplasm, with the patient's age, gender, smoking history, disease-free time interval between the diagnosis of the extrathoracic malignancy and that of the lung lesion. In all 83 cases, CT scans were reviewed to confirm the solitary nature, size, and nodular morphology of the lung lesion.RESULTS Of all 83 cases, the mean age was (57.43±15.34) years. There were 51 males and 32 females, with the ratio of 1.59:1. The lesions included solitary metastasis in 43 cases, pulmonary malignant lesions in 33, and benign lesions in seven. Between the primary lung cancers and solitary metastasis groups, there was no significant difference in the gender ratio (1.20:1 vs 2.31:1, x2=0.0209, P>0.05), but there was a significant difference between the mean age (62.48±11.96 years vs 54.10±16.49 years, t=3.34, P<0.05). in the primary lung cancer and metastasis patient group, the percentage of patients who had a smoking history were 39.3 %(11/17) and 35.9 %(14/39), respectively. Patients with a primary lung cancer had no significant higher frequency of smoking history than did those with a metastatic lesion (x2=0.640, P>0.05). Of 81cases who were followed-up, the mean time of the disease-free interval between extrapulmonary malignancy diagnosis and pulmonary lesion differentiation was 39.73± 6.29 months (range 0～300 months, median 20.00 months), whereas those in the primary lung cancer group and metastatic group were 65.62 ±13.45 months and 22.83 ±4.19 months respectively. This difference was significant between the two groups (Wilcoxon rank sum test, U=2.796, P<0.01). Of all 83 cases, there were ten extrapulmonary squamous carcinomas and 58 adenocarcinomas with ratio of primary lung cancer and solitary metastasis of the tumors were 7:3 and 24:34, respectively (x2 =1.781, P >0.05), without showing a statistically significant relevance between the pathologic patterns of extrapulmonary malignancy and characteristics of the lung nodules. Of all the 83 cases, the mean diameters were (2.77±1.25) cm, whereas the diameters of 33 cases of primary lung cancer and 43 cases of a solitary metastatic lesion were (2.86±1.18) cm and (2.62±1.31)cm, respectively. There was no association between the two groups (t=1.29, P>0.05). There was a statistically significant association between primary lung cancer and the metastatic group with spiculate and smooth edges of the lung lesion (x2=8.562, P<0.01; x2=15.220, P<0.001).The study showed that a lung nodule with a spiculatedmargin correlated with a primary lung carcinoma,whereas those nodules with a smooth edge may more frequently show as a metastastic pulmonary lesion. CT-pathologic correlative analyses of hilar and mediastinal adenopathy were reviewed in 37 patients who underwent Iobectomy and thoracotomy. There was no statistical significant difference between the primary lung cancer group and the metastatic group (x2=2.801,P>0.05).CONCLUSION The likelihood of a primary lung cancer versus a metastasis of ETM-SPN smaller than 3 cm mainly depends on the patient's age, free interval between the two tumors and CT morphological characteristics of the lung lesion. This study showed there was no significant relevancy to factors such as gender, smoking history, pathological patterns of the extrapulmonary neoplasm or whether there has hilar or mediastinal adenopathy.     

Association between hsa-miR-34b/c rs4938723 T 〉 C promoter polymorphism and cancer risk： a meta-analysis based on 6,036 cases and 6,204 controls

Objective： Emerging evidence shows that microRNAs （miRNAs） function as tumor suppressors or oncogenes in human carcinogenesis. A single nucleotide polymorphism （SNP） located in the pri-miRNA promoter may affect the processing and expression of mature miRNA. However, previous studies showed conflicting results regarding the association of hsa-miR-34b/c rs4938723 T 〉 C promoter polymorphism with cancer. Therefore, we conducted a meta-analysis to determine the association of polymorphism with cancer risk. Methods： A computerized search of PubMed, Web of Science, and Chinese National Knowledge Infrastructure （CNKI） for publications on hsa-miR-34b/c rs4938723 T 〉 C promoter polymorphism and cancer risk was performed and the genotype data were analyzed in a meta-analysis. Odds ratios （ORs） with 95% confidence intervals （CIs） were estimated to assess the association. Test of heterogeneity, cumulative meta-analysis, sensitivity analysis and assessment of bias were performed in our meta-analysis by STATA software 12.0. Results： There was no significant association between hsa-miR-34b/c rs4938723 polymorphism and overall cancer risk in the comparison models. Moreover, subgroup analysis revealed that the variant CT （OR = 1.19, 95% CI： 1.03-1.37） and CC/CT （OR =1.18, 95% CI： 1.03-2.35） genotypes were associated with an increased risk of hepatocellular carcinoma （HCC） compared with wild-type TT genotype. However, a decreased risk of colorectal cancer （CRC） was found in the genetic model of CCFFF （OR =0.66, 95% CI： 0.47-0.92） and CC/CT-TT （OR =0.67, 95% CI： 0.49-0.93）. Conclusions： The results suggest that hsa-miR-34b/c rs4938723 polymorphism may play an opposite role in different types of cancer based on current studies, which is the main origin of heterogeneity in this metaanalysis. Further large-scale studies and functional studies between this polymorphism and cancer risk are warranted.     

CYP1A1与GSTM1的多态性与原发性肝癌遗传易感性的关系研究

目的 :探讨细胞色素P4 5 0 1A1(cytochromeP4 5 0 1A1,CYP1A1)与谷胱苷肽S -转移酶M1(glu tathioneS transferaseM 1,GSTM 1)的多态性与原发性肝癌遗传易感性的关系。方法 :应用等位特异PCR和多重PCR技术对 5 2例原发性肝癌患者和 10 0名健康对照的CYP1A1和GSTM 1多态性进行分析。结果 :肝癌患者CYP1A1第 7外显子 4 62Val的等位变异频率为 0 .4 6,显著高于正常对照的变异频率(0 .2 2 ) ,病例组GSTM1的纯合型缺失频率 (0 .65 )也显著高于对照组 (0 .4 1) ,携带有CYP1A1Val/Val纯合变异和GSTM1纯合缺失基因型的人患肝癌的风险大大增加 ,前者的比值比 (oddsratio ,OR)及 95 %可信区间 (confidenceinterval,95 %CI)为 4 .13(1.2 8～ 13.35 ) ,后者的OR值及 95 %CI为 2 .72 (1.35～5 .4 6) ,二者联合OR值及 95 %CI为 8.5 0 (1.74～ 4 1.5 0 )。结论 :CYP1A1和GSTM 1的多态是原发性肝癌的遗传易感因素 ,二者的等位变异增加了患肝癌的风险     

NQO1 C609T polymorphism correlated to colon cancer risk in farmers from western region of Inner Mongolia

Objective:To investigate the relationship between NAD(P)H:quinone oxidoreductase 1(NQO1) C609T polymorphism and colon cancer risk in farmers from western region of Inner Mongolia.Methods:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was performed to analyze NQO1 C609T polymorphism from 160 healthy controls and 76 colon cancer patients.Results:Among the colon cancer patients,the incidence of NQO1 T allele(53.29%) was significantly higher than it in control group(33.75%,P<0.001).The individuals with NQO1 T allele had higher risk [2.239(95% CI:1.510-3.321) times] to develop colon cancer than individuals with NQO1 C allele.The incidence of NQO1(T/T)(34.21%) in colon cancer patients was higher than that in control group(15.62%,P<0.001).Odds ratios(OR) analysis suggested that NQO1(T/T) and NQO1(T/C) genotype carriers had 3.813(95% CI:1.836-7.920) times and 2.080(1.026-4.219) times risk compared with wild-type NQO1(C/C) gene carriers in developing colon cancer.Individuals with NQO1(T/T) genotype had 2.541(95% CI:0.990-6.552) times,3.713(95% CI:1.542-8.935) times,and 3.471(95% CI:1.356-8.886) times risk than individuals with NQO1(T/C) or NQO1(C/C) genotype in welldifferentiated,moderately-differentiated,and poorly-differentiated colon cancer patients,respectively.Conclusions:NQO1 gene C609T could be one of risk factors of colon cancer in farmers from western region of Inner Mongolia.     

裸鼠肺癌移植瘤中NRP-1的表达及其意义

Objective:To establish angiogenesis model of xenografts of lung cancer cell in nude mouse and investigate the expression of the neuropilin-1 (NRP-1) protein in tumors and its role in progression and angiogenesis of lung cancer.Methods:Human lung adenocarcinoma cells A549 were analyzed for the expression of vascular endothelial growth factor165(VEGF165)mRNA using RT-PCR in vitro.TWo groups of nude mice were subcutaneously inoculated with A549 at different tumor-loading time.Two groups of xenografts were jdentified by hematoxylin and eosin (HE) staining.their microvessel density (MVD) were analyzed meanwhile.Two groups were analyzed for the expression of NRP-1 protein and their mean absorbency by using immunohistochemistry and automatic image analysis system respectively.Results:A549 expressed VEGF165 mRNA,and xenografts of A549 in nude mice were successfully established and confirmed by HE staining.The atypia of cancer cells and angiogenesis were occurred in two groups.Two groups of MVD were 13.06±1158.23.61±3.11(vessels/mm2)(P＜0.01).NRP-1 protein was expressed in cytoplasm of vascular endothelium cells and partial tumor cells.Two groups of mean absorbency of NRP-1 were 0.1095±O.0228,0.1784±0.0151 (P＜0.01).Conclusion:The angioqenesis models of xenografts in nude mice with lung cancer cell A549 expressing VEGF165 mRNA at different tumor-loading times were established successfully.The expression of NRP-1 protein and MVD were increased with the tumor progression.Our results demonstrate that NRP-1 protein in lung cancer is related to angiogenesis.     

EFFECTS OF SUPEROXIDE DISMUTASE ON SOME BIOLOGICAL CHARACTERISTICS OF LUNG CANCER CELLS IN VIVO AND IN VITRO

The present study observed the effects of superoxide diamutase (SOD) and its Inhibitor, diethyldithiocarbamate (DDC), on the metastasis of Lewis Lung cancer and some biological characteristics of A548 lung cancer cell line. It was found that SOD and DDC inhibited significantly the metastasis of Lewis lung cancer In C57 BL mice, which the effect of DDC was more significant than that of SOD, and decreased the proliferation of A549 lung cancer cell and its transplantation rate in nude mice.     

Menstrual Factors,Reproductive Factors and Lung Cancer Risk:A Meta-analysis

Background and objective Epidemiological studies have suggested that menstrual and reproductive factors may in uence lung cancer risk,but the results are controversial.We therefore carried out a meta-analysis aiming to examine the associations of lung cancer in women with menstrual and reproductive factors.Methods Relevant studies were searched from PubMed database,CNKI,WANFANG DATA and VIP INFORMATION up to January 2012,with no language restrictions.References listed from selected papers were also reviewed.We included studies that reported the estimates of relative risks(RRs) with 95% con dence intervals(CIs) for the association between menstrual and reproductive factors and lung cancer risk.e pooled RRs were calculated a er the heterogeneity test with the so ware Stata 11,and publication bias and sensitivity were evaluated at the same time.Results Twenty-ve articles,representing 24 independent studies,were included in this meta-analysis.Older age at menarche in North America women(RR=0.83;95%CI:0.73-0.94) was associated with a signi cant decreased risk of lung cancer.Longer length of menstrual cycle was also associated with decreased lung cancer risk(RR=0.72;95%CI:0.57-0.90).Other exposures were not signi cantly associated.Conclusions Our analysis provides evidence of the hypothesis that female sex hormones in uence the risk of lung cancer in women,yet additional studies are warranted to extend this nding and to clarify the underlying mechanisms.     

Biological Significance and the Related Molecular Mechanism of Ets1 mRNA Expression in Lung Cancer by Tissue Microarray(TMA)

Objective: To investigate the expressions and molecular mechanism of Ets-1 mRNA, and TGFβ1 and c-Met proteins in the pathogenesis, progression of lung cancer by tissue microarray (TMA) method. Methods: The expressions of Ets-1 mRNA, and TGFβ1 and c-Met proteins were detected in 89 primary lung cancers, 12 lung cancer with lymph-node metastasis and 12 precancerous lesions by FISH(fluorescence in situ hybridization) and immunohistochemical method, and 10 normal lung tissues were used as controls. Results: The expressions of Ets-1 mRNA, and TGFβ1 and c-Met proteins were significantly higher in 89 primary lung cancer than in the control group (P<0.05). The expressions of Ets-1 mRNA, and TGFβ1 and c-Met proteins were related to lymph node metastasis and clinical stages. There was a positive correlation between the Ets-1 mRNA expression and TGFβ1 and c-Met proteins (P<0.05). Conclusion: Ets-1 mRNA, TGFβ1 and c-Met proteins may be related to the pathogenesis, progression and malignant behavior of lung cancer. They may play an important role in prognosis assessment of lung cancer.