应用抗体-胶体金技术对鹅肠嗜铬细胞超微结构的研究

本文应用抗体-胶体金技术,观察了鹅肠道嗜铬(EC)细胞的超微结构。EC细胞以含多形分泌颗粒为特征,颗粒电子密度较高,包膜紧裹粒芯,许多颗粒具有一圆形“空洞”。十二指肠的EC细胞可见分泌颗粒大小不同的两类。盲肠的EC细胞分泌颗粒直径大于十二指肠,而且在细胞的基部多见形成“腿-足”样胞质突起。     

大鼠中枢神经系统碳酸酐酶Ⅳ免疫细胞化学定位研究

碳酸酐酶的活性在调节脑脊液生成中起着重要的作用，并且也影响神经的兴奋性和抽搐发作的敏感性。实验用免疫细胞化学方法对中枢神经系统碳酸酐酶Ⅳ的分布进行了定位研究。结果显示：光镜下碳酸酐酶Ⅳ免疫细胞化学阳性产物存在于大脑各部，间脑，小脑，中脑，脑桥，延髓和脊髓的毛细血管壁；神经胶质细胞以及脉络丛的毛细血管     

太行山猕猴胃窦粘膜肠嗜铬细胞的定量研究

以Ｇｒｉｍｅｌｉｕｓ嗜银法显示太行山猕猴胃窦部肠嗜铬细胞，并用Ｗｅｉｂｅｌ体视学方法对其进行了定量分析。结果：从胃大弯、胃侧壁至胃小弯，细胞的密度依次减小；大多数肠嗜铬细胞分布在粘膜深段，而粘膜浅段细胞密度极低。一些细胞的嗜银颗粒释放到腺腔面，有时见细胞伸出细长突起伸至其它种类细胞，提示肠嗜铬细胞在胃窦部的分布有明显的区域性差异，并可能有外分泌或旁分泌功能。     

205例大肠癌及癌旁粘膜免疫细胞学研究

应用免疫细胞化学技术(双PAP法),采用三种人结肠癌相关抗原的单克隆抗体(MC_5、CL—2、CL—4),对205例大肠癌及癌旁粘膜进行检测,结果显示癌旁移行粘膜相关抗原阳性率介于正常粘膜与不典型增生之间。此结果可能提示癌旁移行粘膜具有癌前病变性质。     

大鼠下丘脑细胞培养中GnRH神经元的免疫细胞化学研究

采用免疫细胞化学方法，对分离培养１、３、５、７ｄ新生大鼠下丘脑促性腺激素释放激素（ＧｎＲＨ）免疫反应性神经元进行了研究。结果发现，在培养的１ｄ即可检出ＧｎＲｈ神经元，ＧｎＲＨ神经元占培养中的神经元的１２．１％￣１４．８％。ＧｎＲＨ神经元多为双极，有些突起末端可见生长锥。还发现ＧｎＲＨ神经元之间及其与其他神经元之间，存在有多种形式的接触。这些资料提示，培养中的ＧｎＲＨ神经元，保留了在体ＧｎＲＨ神经元     

大鼠脊髓P物质受体分布的免疫细胞化学研究

用免疫细胞化学技术对大鼠脊髓和脊神经节内Ｐ物质受体的定位分布进行了系统的研究。结果证明，Ｐ物质受体阳性胞体和树突主要密集地分布于脊髓全长的Ｉ层；此外，还发现Ⅱ层的外侧部出现少量阳性胞体和树突，来自Ⅱ层的阳性树突穿过Ⅱ层后进入Ⅰ层；Ⅲ￣Ⅴ和Ⅹ层也可见中等密度的阳性胞体和树突；Ⅵ层和Ⅶ层仅见少量阳性胞体和树突，但胸髓中间带外侧核、骶髓副交感运动核、骶髓后连合核内可见大量浓染的阳性胞体和树突；Ⅷ、Ⅸ层、     

大鼠下丘脑加压素神经元的免疫细胞化学初步观察

用免疫细胞化学方法,观察到青年和老年大鼠下丘脑室旁核(PVN)视上核(SON)部位加压素免疫反应阳性神经元(VP神经元)较密集出现,并有粗细两类纤维。在PVN,有时可见一些VP神经元胞体或纤维接近或存在于室管膜层,甚至伸入第三脑室内。多数老年鼠PVN的VP神经元胞体染色浅,深染颗粒减少。发自PVN的粗细纤维都显著减少,染色也变浅。但VP细胞数量、形态、大小和青年鼠差不多。在老年鼠SON,VP神经元未见明显改变。     

Effect of reserpine on the enterochromaffin cells of the small intestine

Summary To investigate the mechanism of the reserpine effect on the blood pressure an experiment was done on 8 guinea pigs with a corresponding control. Experimental animals were given reserpine in the doses of 0.05 mg, 0.125 mg and 0.25 mg. No reserpine was administered to control animals.In a month after the reserpine administration was started the animals were sacrified and various portions of their duodenum were subjected to histological examination. The total number of enterochromaffin cells in the duodenum was found to be reduced; the formation of the granules in the protoplasm of chromaffin cells was diminished or these granules were totally lost.A suggestion is made that the drop of arterial pressure occurred as a result of reserpine action upon the enterochromaffin cells of the small intestine, which produce serotonin.(Presented by Active Member AMN SSSR V. V. Parin) Translated from Byulleten Éksperimental'noi Biologii i Meditsiny, Vol. 51, No. 2, pp. 78–81, February, 1961     

Immunocytochemical and electronmicroscopical data on the differentiation of somatostatin-containing endocrine cells in human large intestine

The distribution and ultrastructure of the somatostatin-containing endocrine cells in the distal large intestine of fetuses, children and adults was investigated by silver impregnation, immunocytochemical and electron-microscopical techniques. Somatostatin-containing endocrine cells in 10-14-week-old fetuses were found to differentiate very intensively. In 19-25-week-old fetuses the frequency of occurrence began to decrease and this tendency was preserved during the subsequent stages of ontogenesis. Conversely, the frequency of occurrence of argyrophil endocrine cells increased. The change in the mean diameters of the secretory granules was also studied.     

Undifferentiated carcinoma of the large intestine

Eight cases of the rare undifferented carcinoma of the large intestine are described. The histological distinction between undifferentiated and poorly differentiated colonic adenocarcinoma and malignant carcinoid is discussed. It is concluded that undifferented carcinoma is a variant of adenocarcinoma which tends to grow to a large size before symptoms are produced but which nevertheless has a good prognosis when locally resectable. Five patients survived between 6 and 28 years, one is well 6 months after operation and two cases where local removal could not be achieved, died within a year.     

Pleomorphic carcinoma of the large intestine

Pleomorphic carcinoma, firstly described in the lung, is exceptional in the digestive tract. This tumor is often associated with fever and peripheral blood leukocytosis. The histological feature is characterized by giant pleomorphic often multinucleated cells with dense neutrophil infiltration and frequent phagocytosis of neutrophils by the tumor cells. Expression of epithelial markers confirm the diagnosis. The prognosis is very poor. We hereby report one case of pleomorphic carcinoma unusually situated in the large intestine, emphasizing difficulties encountered in establishing diagnosis.     

Immunocytochemical study of the myoepithelial cells in fibrocystic disease and ductal carcinoma of the breast

Myoepithelial cells (MC) were identified and the pattern of their distribution in dysplasia and duct carcinoma of the mammary gland was studied using a monospecific antiserum to smooth-muscle myosin of the human uterus, by the indirect Coons' method under luminescent microscope. Samples of the operation material from 10 patients with fibrous-cystic mastopathy and 26 patients with duct carcinoma were examined. In solid (7 observations) and comedo (4 observations) carcinomas with the structure of cancer in situ, MC were found intact in the periphery. In invasive growth of these tumors as well as in scirrhus (5 observations) and adenocarcinoma (10 observations) MC disappeared from the duct structures and were absent in carcinoma proliferates. Positive immunofluorescence in the conventional location of MC in the alveolar duct system of the mammary gland allows sufficiently valid identification and study of the distribution and changes in these cells. The immunocytochemical verification of MC in dysplasia and duct carcinoma indicates the possibility of using MC as markers of duct carcinoma in situ; MC disappearance from the duct structures characterizes the beginning of the infiltrative tumor growth.