DNase I足迹法（DNase I footprinting）

一种确定DNA结合蛋白在DNA分子上的结合位点的方法。其原理是：将含有特定顺式元件的双链DNA片段进行单链单末端标记，与初步纯化的DNA结合蛋白在体外行结合作用，然后加入DNase I对DNA一蛋白质复合物进行随机切割，产生一系列不同长度的DNA片段，其相邻片段只相差一个核苷酸。DNA结合蛋白与其特异序列结合后，由于空间位阻等多种效应，DNase I不能对其进行切割，     

CPU—I呼吸机故障分析

ＣＰＵ—Ｉ呼吸机是一种用微电脑控制的呼吸机，微电脑程序表提供了８种呼吸模式，供临床上选用最佳的呼吸支持用于各种特殊病人。适用于成人与小孩的抢救、呼吸治疗等。各种通气模式的控制是通过调节Ｔｉ、Ｔｐ、Ｔｅ的时间来实现的，各种参数通过微电脑监测并显示，该呼吸机还设有多项报警及校准系统。ＣＰＵ—Ｉ呼吸机主控电路采用２８０—Ａ单板极，我院于１９８７年购买了两台ＣＰＵ－Ｉ呼吸机，当时尚属较贵重仪器，现将几例较特殊故障介绍给同行。故障现象一：“ＶＥＮＴＩＬＡＴＯＲＯＦＦ”显示闪动。故障分析；此故障显示提示呼吸…     

I’ll Pick Forgiving

In this essay, the author gives voice to turning points in her life related to infidelity, mental illness, and forgiveness. By melding poetry, prose, dream journal entries, and medical case notes, she tells the story of how she became a single parent of a young man with mental illness. This narrative constitutes a defining moment in which she reframes an unforgivable relational transgression as an unforgettable opportunity for forgiveness.     

Should I stay or should I go? Hospital emergency department waiting times and demand

In the absence of the price mechanism, hospital emergency departments rely on waiting times, alongside prioritisation mechanisms, to restrain demand and clear the market. This paper estimates by how much the number of treatments demanded is reduced by a higher waiting time. I use variation in waiting times for low‐urgency patients caused by rare and resource‐intensive high‐urgency patients to estimate the relationship. I find that when waiting times are higher, more low‐urgency patients are deterred from treatment and leave the hospital during the waiting period without being treated. The waiting time elasticity of demand for low‐urgency patients is approximately ?0.25 and is highest for the lowest‐urgency patients.     

^131I同位素治疗甲亢

甲状腺功能亢进(简称甲亢)是指甲状腺功能增高、分泌甲状腺激素增多所引起的常见内分泌疾病。其特征有甲状腺肿大、基础代谢增加和自主神经系统的失常。该病多见于女性,以20～40岁最多见,病人常有情绪易激动、精神过敏、多言多动、失眠、紧张、思想不集中,胃口好而人消瘦、心率加快、怕热多汗和突眼等症状。得了甲亢该如何治疗?目前医学上主要有内科抗甲状腺药物治疗、外科手术治疗和同位素~(131)碘(~(131)I)内放射治疗三种,三种治疗方法各有优缺点。在以往用抗甲状腺药物治疗应用最广泛,但仅能获     

环境因素与儿童I型糖尿病

本文将近几年关于环境因素与儿童Ｉ型糖尿病关系研究情况作一概述。上前认为，肠道病毒感染，尤其柯萨奇Ｂ４和Ｂ５感洒是本病高危险易患因素，亚硝胺作为ＩＤＤＭ病因，虽处于研究妆期，但已证明和促发Ｉ型糖尿病有关。牛乳清蛋白因为和人体胰岛素Ｐ＾６９多肽具有同源性，故有可能是本病发病的促发因素，但尚需进一步大量重复性实验结果的证实。