根据Ⅰ型胶原氨基端末肽、Ⅰ型胶原羧基端末肽、骨钙素及骨特异性碱性磷酸酶早期诊断骨不连

BACKGROUND: Detecting the serum levels of biochemical markers to assess bone fractures is simple, mini-invasive and specific. Thereafter, to predict bone nonunion by choosing an appropriate marker has become a hotspot. OBJECTIVE: To establish an animal model of bone nonunion and explore the changing rules of the biochemical markers during the process of nonunion. METHODS:Twenty New Zealand white rabbits aged 5-6 months were enrolled and divided into two groups. In bone defect group, a 15-mm length of bone (including the periosteum) was removed from the left mid-radius, and the medullary cavities were closed with bone wax. In bone fracture group, the mid-radius was fractured. X-ray examination was taken and blood samples were extracted preoperatively and at 2, 3, 4, 5, 6, 7, 8, 10, and 12 weeks after surgery. The serum levels of osteocalcin and bone-specific alkaline phosphatase (BSAP) as markers of bone formation, and C-terminal telopeptide of type I collagen (CTX), N-terminal telopeptide of type I collagen (NTX), and tartrate-resistant acid phosphatase 5b (TRACP 5b) as markers of bone resorption, were measured using biotin double-antibody sandwich ELISA. RESULTS AND CONCLUSION:In the bone defect group, the bone metabolism was at a high level, suggesting that the early diagnosis of bone nonunion depends on several biochemical indicators. In the bone defect group, the serum level of CTX peaked at 5 weeks, and the serum levels of osteocalcin, BSAP and NTX decreased obviously at 4 or 5 weeks, while the serum TRACP 5b concentration did not change significantly, indicating that all above markers except TRACP 5b sensitively reflect the bone turnover in vivo. Further studies are needed to determine whether systematic monitoring of the biochemical markers can reflect the bone turnover effectively and can be used for the early diagnosis of nonunion in the rabbit model. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

骨Ⅰ型胶原交联肽──骨吸收的新指标

骨Ⅰ型胶原（ＢＩＣ）中的交联物质及其肽是ＢＩＣ的降解产物，已成为骨吸收特异性新指标，正在临床应用中进行广泛评价。本文综述其生化特性，测定方法，以及在代谢性骨病和骨质疏松症中的诊断、治疗监测及正常骨代谢研究中的应用。     

乙肝患者血清Ⅲ型前胶原肽测定的临床意义

我们对临床上明确诊断的 10 8名乙肝患者进行了血清Ⅲ型前胶原肽 (ＰⅢＰ)测定 ,并就临床意义作初步分析 ,现报告如下。对象和方法一、对象 :(一 )正常人 :35人。均为我院门诊体检合格的正常人 ,无心、肝、肺、肾等重要脏器疾患 ,肝、肾功能试验正常。(二 )病人组 :10 8人 ,均为临床上明确诊断的乙肝患者 ,其诊断标准符合 1984年南宁全国病毒性肝炎会议修订的防治方案 (其中急性肝炎 31人 ,慢性肝炎 2 1人 ,慢活肝 2 6人 ,肝硬化 30人 )。二、方法 :放射免疫分析试剂盒由海军医学研究所提供 ,操作按说明书。结　　果一、正常人和各型乙肝患…     

83例人Ⅲ型前胶原肽RIA

以前肝纤维化的诊断主要通过创伤性的肝活体组织检查来诊断，既局限又不能反复检测，近几年来国内很多作者采用了血清Ⅲ型前胶原肽的放射免疫测定。可反复检测血清浓度进行动态观察。     

肝炎患者血清透明质酸、Ⅲ型前胶原氨基端尾肽、层粘蛋白与Ⅳ型胶原的检测及其临床意义

分析了166例肝病患者血清透明质酸（HA）、Ⅲ型前胶原氨基端尾肽（PⅢNP）、层粘蛋白（Ln）与Ⅳ型胶原（Ⅳ－C）。结果是：HA、PⅢNP、Ln在各型肝病人中血清含量均高于正常组（P＜0．01），IV－C在除急性肝炎组外的其余各组血清含量均高于正常组（P＜0．05）。而HA、PⅢNP、Ⅳ－C的含量，慢活肝、肝硬化组的病人高于慢迁肝组的病人（P＜0．01）。肝硬化组病人Ln高于慢迁肝组病人（P＜0．05）。HA、PⅢNP、Ⅳ-C、Ln与SB、ALB有相关性，而与ALT无关，慢活肝组病人PⅢNP平均值高于肝硬化组的病人。提示这些肝硬化指标在反映肝纤维化程度上有实用意义，似乎PⅢNP更能功态反映肝纤维化的程度。但这些肝纤维化指标在不同程度上受肝细胞损害的影响。     

Ⅰ型胶原吡啶交联终肽(ICTP)在骨肿瘤鉴别诊断中的意义

目的：检测原发性恶性骨肿瘤、 骨转移性肿瘤患者血清中的Ⅰ型胶原吡啶交联终肽（ICTP）活性, 以评价ICTP在骨肿瘤诊断和鉴别诊断中的意义.方法：对2004年本院骨肿瘤科收治的40例恶性骨肿瘤、 14例骨转移性肿瘤患者, 采用酶免疫测定（EIA）方法测定ICTP活性.结果：恶性骨肿瘤组的ICTP活性为（16.33±11.82） μg/L, 与健康对照组比较有统计学差异（P＜0.01）.而骨转移性肿瘤组ICTP活性为（8.97±6.08） μg/L与对照组相比无统计学意义（P＞0.05）.两组骨肿瘤组间也有统计学意义（P＜0.05）.结论：血清ICTP是反映骨肿瘤骨代谢的一个灵敏而简便的检测指标, 并对骨肿瘤的诊断及鉴别诊断具有一定的临床意义.     

血清Ⅰ型胶原C端肽与性别及年龄的关系

近年来,骨转移生化标志物以其较高的敏感性和特异性在临床骨代谢性疾病及恶性肿瘤骨转移的诊断与治疗中得到了普遍重视和应用。其中,血清Ⅰ型胶原C端肽（β-CTx）是最具代表性的骨吸收标志物之一。本文就该指标与性别及年龄的关系进行了初步探讨。     

慢性乙型肝炎(中度)患者血清HA、Ⅲ型前胶原氨基端尾肽检测的临床意义

本文采用血清ＨＡ和Ⅲ型前胶原氨基端尾肽对慢性肝病早期纤维化病人进行了联检 ,并就临床价值作初步探讨。现将结果报告如下。对象和方法一、对象 :（一）正常人 :３０人（男１９ ,女１１）平均年龄３６．２岁 ,均为我院体检合格的健康人。无心、肝、肺、肾等重要脏器疾患 ,肝肾功能试验正常。（二）病人组 :８３人为我院明确诊断的慢性乙型肝炎（中度）。其中部分病例经肝穿刺病理诊断证实。８３例病人中有慢性肝炎活动期４３例 ,慢性肝炎稳定期４０例。其诊断标准符合１９９０年全国第六次病毒性肝炎学术会议制订的分型标准。二、方法 :（…     

Ⅰ型胶原N末端肽和定量CT骨密度早期测定实验性骨不连

BACKGROUND: Currently, X-ray examination is mainly used for diagnosis of nonunion. However, this method that relies only on the clinician’s experience and degree of callus mineralization has less accuracy because it is vulnerable to projection, processing conditions and subjective factors. OBJECTIVE: To establish an animal model of nonunion and to detect the variation of biochemical markers and bone mineral density.   METHODS: Twenty New Zealand white rabbits were randomly divided into two groups, and bone defect and fracture models were made in the midshaft of the forearm radius, respectively. X-ray examination of the forearm, quantitative CT measurement of bone mineral density and serological test were carried out before and at 2, 3, 4, 5, 6, 7, 8, 10, 12 weeks after surgery. RESULTS AND CONCLUSION: Postoperative X-ray films showed that the in the bone defect group, a little callus formed in three rabbits at 2 weeks, the callus formed stably at 5 weeks, but there was still no healing at 8 weeks; in the fracture group, the fracture line was blurred at 2 weeks and a large number of calluses formed at 6-8 weeks. Compared with the fracture group, the value of bone mineral density in the bone defect group began to decrease significantly at 5 weeks after surgery. Results from the serological test showed that in the bone defect group, the activity of bone-specific alkaline phosphatase increased after surgery, reached peak at 4 weeks, began to decrease at 5 weeks and became stable at 6 weeks; the activity of tartrate-resistant acid phosphatase 5b increased after surgery, peaked at 4 weeks, then decreased and stabilized basically; the expression of N-terminal telopeptide of type I collagen decreased significantly at 5 weeks after surgery and became stable at 6 weeks. These findings indicate that the systematic monitoring of changes in bone mineral density and biochemical indicators such as bone-specific alkaline phosphatase, tartrate-resistant acid phosphatase 5b and type I collagen N-terminal telopeptides may help to reflect the early progress of rabbit nonunion.       

肝炎患者血清透明质酸,Ⅲ型前胶原氨基端尾肽,层粘蛋白？…

分析了１６６例肝病患者血清透明质酸（ＨＡ），Ⅲ型前胶原氨基端尾肽（ＰⅢＮＰ），层粘蛋白（Ｌｎ）与ＩＶ型胶原（ＩＶ－Ｃ）结果是：ＨＡ－ＰⅢＮＰ，Ｌｎ在各型肝病人中血清含量均高于正常组（Ｐ〈０．０１），Ⅳ－Ｃ在除急性肝炎组外的其余各组血清含量均高于正常组（Ｐ〈０．０５），而ＨＡ，ＰⅢＮＰ，Ⅳ－Ｃ的含量，慢活肝，肝硬化组的病人高于慢迁肝的病人（Ｐ〈０．０１），肝硬化组病人Ｌｎ高于慢迁肝组病人（Ｐ〈０．０     

I型胶原吡啶交联终肽(ICTP)在骨肿瘤鉴别诊断中的意义

目的检测原发性恶性骨肿瘤、骨转移性肿瘤患者血清中的I型胶原吡啶交联终肽(ICTP)活性,以评价ICTP在骨肿瘤诊断和鉴别诊断中的意义。方法对2004年本院骨肿瘤科收治的40例恶性骨肿瘤、14例骨转移性肿瘤患者,采用酶免疫测定(EIA)方法测定ICTP活性。结果恶性骨肿瘤组的ICTP活性为(16.33±11.82)μg/L,与健康对照组比较有统计学差异(P<0.01)。而骨转移性肿瘤组ICTP活性为(8.97±6.08)μg/L与对照组相比无统计学意义(P>0.05)。两组骨肿瘤组间也有统计学意义(P<0.05)。结论血清ICTP是反映骨肿瘤骨代谢的一个灵敏而简便的检测指标,并对骨肿瘤的诊断及鉴别诊断具有一定的临床意义。     

哮喘患儿血清肾上腺髓质素(ADM)及细胞因子测定的临床意义

研究表明,肺不仅是个呼吸器官,而且还是个具有广泛代谢功能的脏器。它参与许多生物活性物质的合成、释放和代谢。当肺部因病原体的侵入而产生病理改变时,可释放大量的炎性介质,甚至导致人体免疫机制的调节紊乱。为探讨患儿哮喘发病与相关细胞因子的关系,本文测定了30例支气管哮喘患儿血清ADM、ET-1、IL-6及IL-8水平,以探讨本文测定指标与患儿哮喘发病及疗效的关系。现报道如下。     

Ⅰ型胶原交联氨基末端肽(NTX)在转移性骨肿瘤中的表达和临床价值

转移性骨肿瘤是恶性肿瘤通过血液循环或淋巴系统转移到骨骼所产生的继发性肿瘤。近年来,随着癌症发病率的上升以及癌症诊疗水平提高后病人生存期的延长,转移性骨肿瘤的发病率也呈逐年上升的趋势。尸检证明70%~80%的肿瘤均可发生骨转移。目前转移性骨肿瘤已成为影响癌症病人生存质量、威胁病人生命的主要问题。     

严重急性呼吸综合征(SARS)患者血清Ⅳ型胶原含量测定及其临床意义

为了观察SARS患者血清Ⅳ型胶原含量变化，本文用RIA检测了66例SARS患者血清Ⅳ型胶原含量，并与对照组比较。结果表明：恢复期的SARS患者Ⅳ型胶原含量明显高于对照组，恢复期患者血清Ⅳ型胶原含量高于正常值的百分率为28．7％(19／66)。X线胸片病灶吸收缓慢的SARS患者Ⅳ型胶原含量较高。本文提示SARS患者Ⅳ型胶原含量增高，与肺部病灶吸收和纤维化程度有关。因此动态观察血清Ⅳ型胶原含量，可能是监测肺纤维化的较好指标之一。     

89例2型糖尿病患者诊断中血清CRP、HbA1 C和铁蛋白测定的临床分析

糖尿病(DM)的最重要并发症是心血管的系统病变.DM引起的微循环病变、心肌病变、动脉硬化等疾病已上升为主要并发症.Yaung等研究表明,因DM心脏病引起死亡的患者占DM患者死亡的70%～80%,冠状动脉疾病是导致DM患者死亡的主要原因.DM患者心肌缺血通常无症状,约42%的心肌梗死无痛性,因此发现时冠状动脉病变程度已经相当严重[1～2].