羟基磷灰石义眼台植入术42例临床观察

目的：探讨羟基磷灰石（hydroxyapatite,HA）义眼台植入术的临床疗效。方法：对42例患者采用HA义眼台,行I期或Ⅱ期植入术。结果：术后随访3-24个月,10例（占23.8％）发生术后并发症,其中义眼台暴露8例、术后感染1例、结膜囊狭窄1例,经过对症处理效果满意。结论：正确掌握HA义眼台植入术的手术方法及处理术后并发症,将获得满意的临床疗效。     

双层巩膜缝合羟基磷灰石义眼台植入的临床观察

目的:探讨双层巩膜缝合羟基磷灰石(HA)义眼台植入的方法及临床效果。方法:应用自体巩膜成形、双层巩膜覆盖方法包裹HAⅠ期植入。结果:52例双层巩膜缝合HA义眼台植入术后均达到良好效果。随访3～24个月无义眼台脱出或移位、感染等并发症发生。结论:双层巩膜缝合羟基磷灰石义眼台植入术式操作简单,并发症少,术后义眼座活动度好,临床疗效良好。     

带线羟基磷灰石义眼台植入术中加斜肌缝合术

目的：探讨带线羟基磷灰石（HA）义眼台植入术中加斜肌缝合术的手术效果。方法：带线HA义眼台植入术中四条直肌加上下斜肌同时缝合来加固义眼台10例,其中一期植入3例,二期植入7例。结果：术后随访5-17个月,无1例发生球结膜裂开,感染,义眼台暴露、脱出、移位,上睑凹陷,眼窝凹陷,义眼活动度大。结论：带线HA义眼台植入术中加斜肌缝合术具有义眼台暴露发生率低、义眼活动好等优点。     

HA义眼台眶内植入术后义眼台取出的原因分析

目的 分析羟基磷灰石(HA)义眼台眶内植入术后义眼台取出的原因,探讨预防义眼台取出的有效方法.方法 对1995年9月～2009年6月在我院收治的所有HA义眼台植入术后义眼台取出的35例患者进行回顾性调查.针对不同的病因进行相应治疗,治疗无效后行义眼台取出术.结果 共调查有完全随访资料的35例患者.最终导致义眼台取出的原因分为义眼台暴露20例,义眼台感染6例,脓性肉芽肿9例.其中,9例脓性肉芽肿患者义眼台均未完全血管化.结论 HA义眼台眶内植入术后义眼台取出是严重并发症之一,是多种因素综合影响所致.义眼台血管化不足、义眼台暴露、义眼台感染及脓性肉芽肿是义眼台取出的常见原因.     

羟基磷灰石义眼台暴露的预防和处理

目的：探讨羟基磷灰石（HA）义眼台植入术后大范围暴露并发症的原因及处理方法。方法：对46例HA义眼台暴露患者，根据其结膜伤口和义眼台暴露范围的大小，采用不同的手术方法修补结膜囊。包括直接缝合5例，异体巩膜修补6例，自体真皮移植24例，真皮联合保存羊膜移植11例。其中9例伴有义眼台严重感染的同时行义眼台更换术。结果：所有患者修复术后均取得满意效果，移植组织无溶解排斥，术后3－6周配戴义眼片，随访1－6年无复发。结论：用自体真皮或联合羊膜移植修复大范围HA义眼台暴露，是治疗此并发症的有效方法。强调一期手术的质量及结膜小伤口的早期积极处理，是减少严重并发症的关键。     

羟基磷灰石义眼台植入术的临床效果观察

目的 探讨羟基磷灰石(hydroxyapatite,HA)义眼台植入的临床应用及手术效果.方法 32例病例中6例行眼球摘除联合Ⅰ期HA义眼台植入;19例行眼内容物摘除联合Ⅰ期自体巩膜包裹HA义眼台植入;3例Ⅱ期HA义眼台植入.Ⅱ期植入7例.结果 32例病例术后随访6-72个月,30例术后效果满意.球结膜裂开伤口延迟愈合1例.伤口裂开义眼台轻度暴露1例,义眼台活动度相对稍差2例.结论 眼内容物摘除联合Ⅰ期自体巩膜包裹HA义眼台植入术效果满意.     

眶内二期羟基磷灰石义眼台植入术

目的总结探讨眶内二期羟基磷灰石义眼台植入手术的临床疗效。方法对37例行眼球摘除或眼内容物剜出术后的患者采用带线多孔珊瑚羟基磷灰石（HA）义眼台行二期眶内植入术，观察其外观修复效果。结果术后随访6～18个月，眼部外观满意度达100％，无感染、义眼台排斥现象，并发症主要有术后球结膜裂开、义眼台部分外露等。结论眶内二期羟基磷灰石（HA）义眼台植入术，是眼球丧失后理想的眼眶充填手术。     

无包裹式羟基磷灰石义眼台植入术的效果

目的探讨无包裹式羟基磷灰石（hydroxyapatite,HA）义眼台植入术的效果。方法对42眼施行无包裹式羟基磷灰石义眼台植入术,术中均行眼球筋膜与球结膜分层无张力对位缝合,术后抗炎治疗,观察外观、义眼台活动度情况及并发症的出现情况。结果术后随访6-18个月,42眼HA义眼台居中,义眼与健眼对称,外观满意。HA义眼台活动度优32例（76.19%）,良10例（2.38%）,无HA义眼台脱出。结论无包裹式羟基磷灰石义眼台植入术是较好的手术方法。     

羟基磷灰石义眼台Ⅱ期植入的临床分析

目的 观察眼球摘除术后或眼内容物剜除术后二期眶内植入羟基磷灰石(HA)义眼台的修复效果。方法 32例(32只眼)，眼球摘除后18例行异体巩膜包裹HA义眼台眶内植入，眼内容物剜除后14例采用自体巩膜包埋的HA义眼台眶内植入。结果 术后均出现轻度的局部反应表现，1例拆线前切口裂开，2例上睑沟轻度凹陷，1例上睑下垂，1例下穹隆浅，经保守治疗或手术处理，随访3～11月患者均取得理想的美容和功能效果，无一例发生术后眶内感染或义眼台暴露、脱出。结论 羟基磷灰石义眼台二期植入是安全可靠的手术，能取得理想的临床效果。     

自体巩膜倒扣包裹羟基磷灰石义眼台一期植入术临床观察

目的观察自体巩膜倒扣包裹羟基磷灰石(HA)义眼台一期植入术临床效果、术后并发症及随访预后情况。方法选取我院2012年7月—2014年3月期间26例需植入HA义眼台患者,采用进口材料HA义眼台,直径18~22mm,重量约2g,施行眼球摘除术后,予眼内容剜除,自体巩膜壳倒扣包裹于HA义眼台前端,一期植入肌锥的方法。结果随访2~20个月,2例发生结膜充血水肿(7.69%),3例发生结膜创口愈合不良(11.54%),行保守治疗或于手术室清创缝合后痊愈,余未发生义眼台排斥、暴露、感染、移位及眼球假体固定。结论羟基磷灰石(HA)义眼台具有高度的生物相容性,无毒性、无抗原性,纤维结缔组织可长入内联多孔,不引起明显异物排斥反应,是目前较理想的眼座材料。自体巩膜倒扣包裹HA的应用减少了义眼台植入术后并发症的产生,术后眼窝饱满,眼球假体活动度可,外观恢复满意,临床效果好,是眼眶整形术中较为理想的方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.