马斯平对医院常见致病菌活性实验研究及前景展望

目的　验证马期平 (头孢吡肟 )的抗菌谱 ,研究其在临床应用中的前景及意义。方法　用浓度梯度法E test法测定头孢吡肟对临床分离菌 4 0株的最低抑菌浓度 ,并与其他抗生素体外抗菌活性进行比较。结果　头孢吡肟对肠杆菌科抗菌活性强于第 3代头孢菌素 ,对铜绿假单胞菌的抗菌活性也较强 ,与亚胺掊南相当。对苯唑青霉素敏感的金黄色葡萄球菌较敏感 ,(MIC90 为 3mg/L)。 结论　头孢吡肟对革兰阴性杆菌的抗菌活性比第 3代头孢菌素强 ,且低诱导、低选择、高稳定 ,对革兰阳性球菌也有较强的抗菌覆盖     

多黏菌素B联合不同抗菌药物抗耐碳青霉烯肺炎克雷伯菌和大肠埃希菌的协同作用

目的:观察多黏菌素B与几种抗菌药物联合抗耐碳青霉烯肺炎克雷伯菌和大肠埃希菌的协同作用.方法:16株碳青霉烯类耐药菌中肺炎克雷伯菌11株,大肠埃希菌5株,观察多黏菌素B与利福平、强力霉素、头孢吡肟、亚胺培南、头孢曲松和庆大霉素对上述菌株的协同作用,通过棋盘微量稀释法确定每种抗菌药物的最小抑菌浓度( MIC)值,根据MIC...     

Microdilution inhibition test of Chinese herbs to assess their effect against clinical strains of Ureaplasma urealyticum in vitro

Objective:To explore the antibacterial effect of Chinese crude drugs against clinical strains of Ureaplasma urealyticum(UU), including eight pure herbs and three compound herbs, and determine their minimal inhibitory concentrations(MICs). Methods:Isolates were collected from clinical patients with UU infection, and cultured in UU broth. In order to test the different effects on clinical strains of UU, the assays were performed by microdilution inhibition tests, and MICs of the herbs against the clinical strains of UU were calculated. Results:The MICs of eight pure herbs against clinical strains of UU were as follows: Galla Chinensis:0.313～1.25 g/L;Fructus Forsythiae: 1.25～5.00 g/L;Cortex PheUodendri: 1.25～5.00 g/L;Radix Paeoniae Rubra: 1.25～2.50 g/L;Semen Plantaginis:2.50～10.00 g/L;Herba Lysimachiae: 5.00～20.00 g/L;Rhizoma Coptidis: 5.00～20.00g/L, Herba Houtmyniae: 10.00～20.00 g/L. The MICs of compound herbs were: Liuheji: 0.625～2.50 g/L;Bazhengsan: 5.00～20.00 g/L;Wulinsan:2.50～20.0 g/L.Conclusion:Galla Chinensis, Fructus Forsythiae, Cortex Pheilodendfim, Radix Paeoniae Rubra, and Semen Plantaginis, exerted the stronger antibacterial effect against clinical strains of UU, whereas Herba Lysimachiae, Rhizoma Coptidis and Herba Houttuyniae, had relatively weaker activity against UU. Compound herbs,Bazhengsan and Wulinsan, and particularly Liuheji, also had antibacterial effects against UU. Further studies of the effects and mechanisms of action of Chinese crude drugs against UU infections are worthwhile.     

PV E6/E7 mRNA联合细胞学检查用于子宫颈癌早期筛查的初步评价

目的 探讨人乳头瘤病毒（HPV） E6/E7 mRNA联合液基薄层细胞学检查（TCT） 用于宫颈癌早期筛查的初步评价。方法 收集2015年10月—2019年7月在本院进行宫颈癌筛查患者825例,均进行HPV E6/E7 mRNA、TCT检测, 以组织病理学为金标准,分析HPV E6/E7 mRNA与TCT联合检测用于评估患者宫颈病变风险的诊断效能。结果 HPV E6/E7 mRNA 检测和TCT检测不同病理分级患者宫颈慢性炎、低级别鳞状上皮内病变（LSIL）、高级别鳞状上皮内病变（HSIL）、宫颈癌的阳性率分别为12.17%、39.04%、86.61%、87.88%和36.51%、82.89%、82.14%、81.82%。HPV E6/E7 mRNA检测的敏感性为86.90%,特异性为80.44%,准确性为81.58%。TCT检测的敏感性为82.07%,特异性为50.74%,准确性为56.24%。HPV E6/E7 mRNA联合TCT检测的敏感性为70.34%,特异性为83.68%,准确性为81.33%。HPV E6/E7 mRNA与TCT联合检测特异性高于单一检测,差异有统计学意义（P＜0.001）。HPV E6/E7 mRNA与TCT两种检测方法的受试者工作特征曲线（ROC）曲线下面积（AUC）分别为0.837和0.664,两者联合检测的AUC为0.860,差异性显著（P＜0.001）,提示联合诊断可提高诊断宫颈HSIL的性能。结论 HPVE6/E7 mRNA 联合TCT能够更好预测宫颈病变的进展,且针对高级别病变的筛查具有更高的特异性,可为临床宫颈癌筛查提供参考     

液基薄层细胞学检查联合人乳头瘤病毒E6/E7 mRNA检测在宫颈病变筛查中的价值分析

目的 探讨薄层液基细胞学检查（thinprep cytology test,TCT）联合人乳头瘤病毒（human papillomavirus,HPV） E6/E7 mRNA检测在宫颈疾病筛查中的应用效果。方法 选取538例患者作为研究对象,所有患者均行TCT、HPV E6/E7检测和阴道镜下宫颈多点活检,比较单独使用TCT或HPV E6/E7 mRNA以及联合TCT和HPV E6/E7 mRNA检测在宫颈病变筛查中的诊断价值。结果 以病理学检查作为检测金标准,TCT和HPV E6/E7这两种检测方法对于宫颈病变均具有一定的筛检率,TCT检测的灵敏度66.56%,特异度为53.57%,HPV E6/E7 mRNA检测的灵敏度74.84%,特异度为77.68%,TCT联合HPV E6/E7 mRNA检测的灵敏度和特异度分别为99.68%和39.29%。结论 TCT联合HPV E6/E7 mRNA检测在宫颈病变筛查中可大幅度提高灵敏度,降低临床漏诊率,具有临床筛查价值。