紫杉醇热化疗对人红白血病细胞株K562的细胞毒作用

目的 探索不同热疗温度、时间、药物浓度对紫杉醇细胞毒作用的影响.方法 K562细胞转染绿色荧光蛋白(GFP)质粒后,经筛选,将稳定高表达GFP的K562细胞作为靶细胞,分别在热疗时间固定、温度固定、加热与紫杉醇不同顺序情况下比较紫杉醇对K562细胞的毒性作用.结果 加热(40 ℃～45 ℃)30 min左右明显增强紫杉醇的化疗作用,热疗与化疗同时进行时细胞毒指数(CI)显著增高.结论 在细胞水平上证实了加热联合化疗可起到对癌细胞的协同杀伤作用,热疗与化疗同时进行时效果最好.     

热疗与紫杉醇联合应用对H446细胞生长和MMP-2 mRNA表达的影响

目的：探讨单纯热疗、单纯化疗以及热化疗联合应用对H446细胞生长及MMP-2mRNA表达的影响。方法：以不同温度（39℃、40℃、41℃、42℃、43℃和44℃）、不同剂量紫杉醇（30μg／L、60μg／L、120μg／L、240μg／L和480μg／L）及热化疗联合的方法处理H446细胞，同时设对照组（0μg／L紫杉醇，37℃），每组又分为3个热疗时间（30min、60min和90min），观察热疗、化疗和热化疗联合对H446细胞生长抑制率的影响；另取H446细胞，分为单纯热疗组（43℃，90min），单纯紫杉醇化疗组（60μg／L、120μg／L和240μg／L），43℃热疗90min联合紫杉醇组（60μg／L、120μg／L和240μg／L），对照组（0μg／L、37℃），采用RT—PCR检测各组细胞MMP-2mRNA的表达。结果与结论：单纯热疗与紫杉醇均可抑制H446细胞的生长，热疗不同程度地增强了紫杉醇对H446细胞的增殖抑制率，热化疗联合对H446细胞生长抑制的最优组合是43℃热疗90min联合120μg／L紫杉醇化疗，在此条件下，H446细胞MMP-2mRNA的表达明显降低，提示热化疗联合对H446细胞增殖的抑制作用可能是通过降低MMP-2mRNA的表达，从而抑制肿瘤细胞的侵袭和转移而实现。     

紫杉醇与褪黑素联合使用对HeLa细胞的生长影响研究

目的；研究紫杉醇与褪黑素联合应用对ＨｅＬａ细胞的生长影响。方法；通过生长曲线，集落形成试验观察二者联合应用对ＨｅＬａ的影响作用。结果；单独应用紫杉醇和褪黑素对二者联合应用其生长曲线，集落形成试验的结果无明显差别。结论：紫杉醇与褪黑素联合应用对ＨｅＬａ细胞的生长影响与单独应用二者无明显判别。     

丝裂霉素C热化疗对人肝癌细胞-7721的细胞毒作用

目的：探讨比较丝列霉素C（MMC）化疗与加热化疗对人肝癌细胞（HCC－7721）的细胞毒作用．方法：以体外培养的HCC－7721为研究对象，采用水浴加温法，体外细胞毒试验（MTT法），观察了MMC化疗与加热化疗对细胞的生长抑制规律的影响二结果：①43℃30min以上的单独热疗对细胞具有较强的细胞毒作用．②热疗能增强肿瘤细胞对MMC的敏感性，热与MMC联合应用的效果强弱依次为热化同时（HM组）优于先热后化（H→M组）或先化后热（M→H组）．结论：加热可以显著提高丝裂霉素C对HCC－7721的细胞毒作用．     

温热增强人肺癌细胞PLA-801D对多西紫杉醇的敏感性

目的探讨不同温热对多西紫杉醇增敏的规律及其机制。方法应用四氮唑蓝（MTT）快速比色法测定不同温度下的热疗组、单纯化疗组以及以不同序贯方式结合的热化疗组对人肺巨细胞癌细胞（PLA-801D）的生长抑制率;流式细胞仪测定42％温热条件下各组细胞周期的变化。结果1）38～41℃的单纯热疗促进了肺癌细胞的生长（P〈0．01）;42～43℃的单纯热疗对肺癌细胞有抑制和杀伤作用（P〈0．01）;2）温热与药物同时作用效果最佳（P〈0．001）,且抑制率与温度的变化呈正相关（r=0．9920,P=0．0001）;3）细胞周期分析发现热化同时组细胞各个周期增减的幅度最大。结论临床热疗不宜单独进行,需与化疗联合应用;一定范围内,温度越高热化疗的疗效越好。     

丁硫氨酸亚砜胺逆转多重抗药细胞的抗性作用及其作用机理的研究

应用阿霉素诱导的人肺腺癌Ａ５４９多重抗药细胞Ａ５４９／Ｒ２，对丁硫氨酸亚砜胺（ＢＳＯ）逆转多重抗药细胞的抗性作用及其作用机理进行了初步探讨。研究发现：非细胞抑制剂量ＢＳＯ能使Ａ５４９／Ｒ２细胞的ＩＣ５０值显著下降（Ｐ＜０．０１）；大剂量ＢＳＯ能直接抑制癌细胞增殖，且对抗药细胞更有效；Ａ５４９／Ｒ２细胞内总体谷胱甘肽（ＧＳＨ）含量和谷胱甘肽Ｓ－转移酶（ＧＳＴ）活性均非常显著地高于其敏感细胞（Ａ５４９／Ｐ）水平（Ｐ＜０．０１）；ＢＳＯ能使Ａ５４９／Ｒ２细胞内总ＧＳＨ含量和ＧＳＴ活性非常显著地下降（Ｐ＜０．０１）。结果表明：ＢＳＯ能有效地逆转Ａ５４９／Ｒ２细胞的抗药性或对其直接产生细胞毒作用；ＢＳＯ阻断细胞内ＧＳＨ合成，降低抗药细胞内ＧＳＨ水平或干扰ＧＳＴ活性，是对抗药细胞产生抗药性逆转或发挥直接杀伤效应的重要原因。     

紫杉醇联合热疗对鼻咽癌CNE-1细胞株增殖及凋亡的影响

目的 探讨紫杉醇(Paclitaxel)联合热疗对鼻咽癌细胞株CNE-1增殖及凋亡的影响,为临床紫杉醇与热疗联合应用提供实验依据.方法 将不同温度(39℃、41℃和43℃)及不同浓度(0.01、0.10、1.00、10.00和100.00 nmol/L)紫杉醇采用分组设计(共15个组),各浓度组设37℃为对照组(共5个组).在紫杉醇对CNE-1细胞作用48h后加热30min.使用MTT法、克隆形成能力试验、细胞形态学、超微结构观察及琼脂糖凝胶电泳等方法 测定紫杉醇联合热疗对CNE-1细胞体外增殖抑制及细胞凋亡的影响.结果 加热41℃组细胞增殖率与其他不同温度同剂量组比较均降低,差异有显著性.与其他各组比较均降低,差异均有显著性(P<0.05);39℃和43℃组细胞增殖率与37℃对照组比较均升高,差异有显著性(P<0.05).琼脂糖凝胶电泳显示紫杉醇作用48h后便可出现DNA梯带,并呈时间剂量性增强.最低0.10nmol/L浓度可见DNA梯带.倒置显微镜和电镜下可见细胞损伤及凋亡细胞.结论 加热41℃时不同浓度紫杉醇对CNE-1细胞均有增殖抑制及诱导细胞凋亡作用;39℃或43℃热疗联合0.01 nmol/L紫衫醇化疗时CNE-1细胞增殖能力最强.     

TNF与卡铂联合应用对A549肺癌细胞系细胞毒作用的研究

采用＾３Ｈ－ＴｄＲ掺入试验及台盼蓝活细胞拒染法观察了肿瘤坏死因子（ＴＮＦ）与卡铂联合应用对Ａ５４９肺癌细胞的细胞毒作用，实验分为空白对照组，ＴＮＦ组（５００Ｕ／ｍｌ），卡铂组（４０μｇ／ｍｌ）ＴＮＦ（５００Ｕ／ｍｌ）加卡铂（４０μｇ／ｍｌ）组＾３Ｈ－ＴｄＲ掺入试验结果表明，联合用药增强了药物对Ａ５４９细胞ＤＮＡ合成的 制作用，抑制率为６９．３％，而ＴＮＦ组与卡铂组的抑制率则分别为２５．１％和５６．     

热疗联合DDP诱导大肠癌细胞凋亡和bcl—2表达的实验 …

目的：研究热化疗诱导大肠癌（ＬｏＶｏ）细胞凋亡及其与ｂｃｌ－２基因表达的关系，探讨热化疗治疗大肠癌的机制。方法：应用热疗联合顺铂（ＤＤＰ）在不同药物浓度、时间和温度条件下诱导ＬｏＶｏ细胞凋亡，以流式细胞仪检测其凋亡率及ｂｃｌ－２基因的表达。结果：随着药物浓度的升高、作用时间的延长，细胞凋亡率增加；热疗联合ＤＤＰ所致凋亡率大于单纯化疗组和单纯热疗组之和；随着药物浓度的升高，ｂｃｌ－２的表达下凋；各处     

热疗联合紫杉醇对NCI-H446细胞增殖和VEGF mRNA表达的影响

目的:观察热疗联合紫杉醇对NCI-H446细胞增殖作用以及VEGF mRNA表达的影响。方法:将NCI-H446细胞分组,Ⅰ热疗组(43℃加热90 min);Ⅱ紫杉醇组;Ⅲ热疗联合紫杉醇组;Ⅳ对照组(常规培养细胞)。分别在处理72 h后用MTT法检测4组细胞增殖情况;RT-PCR检测各组细胞中VEGF mRNA的表达量。结果:加热抑制了NCI-H446细胞的增殖;单独应用紫杉醇随着药物浓度的倍比增大,增殖抑制率逐渐增大;加热联合紫杉醇与对应浓度单独使用比较差异有统计学意义(P<0.05)。加热降低了NCI-H446细胞中VEGF mRNA的表达;加热联合紫杉醇组中NCI-H446细胞中VEGF mRNA的表达量明显减少,与对应浓度紫杉醇组比较差异有统计学意义(P<0.05)。结论:43℃加热90 min联合紫杉醇对NCI-H446细胞增殖有抑制作用,并降低了VEGF mRNA的表达量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.