Cold coagulation therapy in the treatment of histologically diagnosed subclinical human papilloma virus (HPV) infection of the cervix.

The efficacy of cold coagulation therapy in the treatment of histologically diagnosed subclinical human papilloma virus (HPV) infection of the cervix, was studied among 65 patients who also had genital warts. This therapy was found to be successful as the failure rate was only 8%. There were no serious side-effects. The treatment can be administered easily in an out-patient setting after histological confirmation. This study also showed that if, on the other hand, a subclinical HPV infection is not treated, it may develop into a clinical disease (genital warts) within two years. Therefore long-term follow-up of this condition may be necessary in untreated cases.     

Human papilloma virus (HPV) infections related to cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma of the uterine cervix

Human papilloma viruses (HPV) consist of a heterogenic group of viruses (32 different HPV types identified to date) known to induce a variety of squamous cell tumours (papillomas and warts) in the skin, and on mucous membranes of respiratory, gastrointestinal and genitourinary tracts. In the genital tract, the venereal wart (Condyloma acuminatum) has been recognized since ancient times, and known to be a sexually transmitted disease (STD). In 1976, two other morphologically distinct HPV lesions were described in the uterine cervix, known currently as a flat and an inverted condyloma. Subsequently, these new HPV lesions were shown to be frequently associated with concomitant cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS) lesions, and occasionally with invasive cervical carcinomas as well. These morphological findings, substantiated by the increasing number of reports of malignant transformation of HPV lesions, as well as data from animal experiments and epidemiological surveys, have lent support to the concept that HPV might be involved in the development of cervical (and other) human squamous cell carcinomas. Further evidence has been provided by the recent discoveries of HPV structural proteins (viral antigens) and HPV type 11 DNA in lesions of CIN, as well as HPV 16 and 18 DNA predominantly in invasive cervical carcinomas. So far, HPV 16 and HPV 18 are the only HPV types with DNA shown to exist integrated in the host cell DNA. At present, cervical (and other) HPV lesions are the subject of intense study utilizing epidemiological, morphological, immunohistochemical, biochemical and molecular biological methods (recombinant gene technology) to provide further evidence of the suggested causal relationship between HPV and cancer. Prospective follow-up studies are also in progress to explore the natural history of cervical HPV lesions as well as the factors (immunological, epidemiological, synergistic actions, etc.) which modify it. Despite the rapid progress made in papilloma virus research in the last few years, many important questions have still to be answered before the final conclusions can be drawn as to the possible role of HPV in cervical carcinogenesis.     

Roche AMPLICOR human papilloma virus (HPV) and LINEAR ARRAY HPV tests will profit from automated DNA extraction

BACKGROUND: Persistent infection with high risk human papillomavirus (HR-HPV) types is at the origin of cervical cancer in women and HPV-genotyping is considered to be a valuable tool for risk stratification. Its diagnostic relevance will further increase in the context of HPV vaccination which covers the HR-HPV types 16 and 18 only. For rapid screening and genotyping the commercial Roche AMPLICOR and the Roche LINEAR ARRAY (LA) HPV PCR tests are available. However, the manual DNA extraction procedure recommended by the manufacturer is time-consuming and labor-intensive. To improve workflow we evaluated automated DNA extraction by the MagNA Pure LC (MP-LC). CONCLUSION: The AMPLICOR and the LA HPV tests perform equally well with both extraction methods. The MagNA Pure LC provides an automated alternative for processing HPV clinical specimens coupled with the Roche AMPLICOR and LA HPV tests.     

鼻内翻性乳头状瘤中HPV感染状况分析

目的 分析鼻内翻性乳头状瘤（NIP）中人乳头状瘤病毒（HPV）感染的情况,为进一步研究HPV介导NIP发病、复发及恶变机制提供实验及理论依据。方法 应用核酸分子快速导流杂交基因芯片分型技术（HybriMax）检测NIP实验组（48例）及正常鼻黏膜对照组（20例）中HPV的感染率及其各亚型的表达情况。结果 NIP实验组组织标本中HPV总阳性率为64.58%(31/48),正常鼻黏膜对照组组织标本中HPV检测结果均为阴性,2组阳性率比较差异有统计学意义（χ2=32.178,P <0.01）。在31例HPV阳性标本中共检测出HPV病毒49株,含12个亚型,其中HPV6占4.08%(2/49)、HPV11占32.65%(16/49)、HPV16占12.24%(6/49)、HPV18占4.08%(2/49)、HPV33占4.08%(2/49)、HPV39占2.04%(1/49)、HPV44占10.20%(5/49)、HPV52占8.16%(4/49)、HPV53占2.04%(1/49)、HPV56占2.04%(1/49)、HPV58占16.30%(8/49)、HPV68占2.04%(1/4...     

人类乳头瘤病毒感染与白细胞相关性分析

目的探讨人类乳头瘤病毒(HPV)感染及分型与白细胞的相关性,并研究本地区HPV基因型的分布情况。方法对武汉大学人民医院妇科2011年7月至2013年1月住院的273例患者进行血常规检查,同时进行HPV基因分型检查。结果 HPV阳性患者的白细胞总数及中性粒细胞百分比明显高于HPV阴性患者,差异有统计学意义(P<0.05);HPV阳性患者的淋巴细胞百分比明显低于HPV阴性患者,差异有统计学意义(P<0.05);HPV阳性患者的单核细胞百分比与HPV阴性患者之间没有差别。HPV阳性患者各亚型间的白细胞及分类没有差别。本地区HPV感染的亚型主要为HPV16、HPV33、HPV52,高危亚型感染比例较大(88.8%)。结论白细胞检测结合HPV亚型的检测能提高对HPV感染的诊断率,为采取针对性措施降低宫颈癌的发生率提供支持。     

包皮环切术对减少男性高危型人乳头瘤病毒感染的作用研究

目的 了解包皮环切术在减少成年男性高危型人乳头瘤病毒(HPV)感染中的作用.方法 通过对128例已婚成年男性包皮环切术前及术后2年尿道分泌物高危型HPV的检测,观察手术前后高危型HPV感染率的变化,并同期选择128例包皮过长而未行包皮环切术的已婚成年男性为对照组,比较2组高危型HPV感染率的差异.结果 2组患者入组时高危型HPV的感染率相当,具有可比性.其中包皮环切术组术前高危型HPV的感染率为27.3%,术后2年复测高危型HPV的感染率降为12.5%,差异有统计学意义(x2=8.839,P=0.005);对照组第1次检测高危型HPV的感染率为28.1%,2年后再次检测时感染率为25.0%,二者比较差异无统计学意义(x2=0.350,P=0.671).结论 包皮环切术能够明显降低成年男性高危型HPV的感染率,在我国积极推广包皮环切术对预防高危型HPV感染相关疾病的发生可能具有重要作用.

Abstract：

Objective To understand the effect of circumcision in reducing high-risk human papilloma virus (HPV) infection in male adults. Methods One hundred and twenty-eight married adults with phimosis who underwent circumcision enrolled in the case group and high-risk HPV of urethral discharge specimens were detected before and 2 years after operation. A total of 128 cases of phimosis without circumcision were recruited in the control group. High-risk HPV infection rates were compared between the two groups. Results The highrisk HPV infection rates in the two groups were comparable at baseline. In the circumcision group, high-risk HPV infection rate was 27. 3% preoperative and it significantly decreased to 12.5% two years after operation( x2 =8. 839 ,P =0. 005). In the control group,high risk HPV infection rate was 28. 1% at baseline and it was 25.0%two years later, with no significant difference (x2 =0.320,P = 0.671).Conclusion Circumcision can significantly reduce high-risk HPV infection. Promoting circumcision actively in our country may have an important role in preventing high-risk HPV infection.     

包皮环切术对减少男性高危型人乳头瘤病毒感染的作用研究

目的 了解包皮环切术在减少成年男性高危型人乳头瘤病毒(HPV)感染中的作用.方法 通过对128例已婚成年男性包皮环切术前及术后2年尿道分泌物高危型HPV的检测,观察手术前后高危型HPV感染率的变化,并同期选择128例包皮过长而未行包皮环切术的已婚成年男性为对照组,比较2组高危型HPV感染率的差异.结果 2组患者入组时高危型HPV的感染率相当,具有可比性.其中包皮环切术组术前高危型HPV的感染率为27.3%,术后2年复测高危型HPV的感染率降为12.5%,差异有统计学意义(x2=8.839,P=0.005);对照组第1次检测高危型HPV的感染率为28.1%,2年后再次检测时感染率为25.0%,二者比较差异无统计学意义(x2=0.350,P=0.671).结论 包皮环切术能够明显降低成年男性高危型HPV的感染率,在我国积极推广包皮环切术对预防高危型HPV感染相关疾病的发生可能具有重要作用.     

解脲支原体与人乳头瘤病毒感染相关性研究

目的探讨解脲支原体(UU)感染与人乳头瘤病毒(HPV)感染相关性。方法回顾性分析348例来本院妇产科就诊患者,采集宫颈分泌物标本,使用荧光定量PCRABI7300检测仪,对该标同时检测人乳头瘤病毒DNA常见高危型(16、18、31、33、45、52、56、58)及解脲支原体DNA,设置两组数据,第一组以UU DNA阳性为实验组,UU DNA阴性为对照组,对两组数据进行常见高危型HPV DNA检出阳性率进行分析。第二组数据以UU DNA拷贝数大于104为阳性对照,以UU DNA拷贝数小于104为阴性对照,两组数据进行常见高危型HPV DNA检出阳性率进行分析。结果第一组数据UU DNA检出率达到67.5%(235/348),UU阳性HPV DNA检出率为14.89%(35/235),UU阴性HPV DNA检出率7.07%(8/113),两组数据比较,χ2=4.302 3,P0.05,差异有统计学意义。第二组数据UUDNA拷贝数大于104 HPV检出率为17.75%(30/169),UU DNA拷贝数小于104HPV检出率为7.57%(5/66),两组数据比较χ2=3.877 3,P0.05,差异有统计学意义。结论解脲支原体的感染与人乳头瘤病毒的感染具有相关性,UU感染会增加HPV感染机会,且随着UU含量的增加而增加。     

某城区女性人乳头瘤病毒感染状况调查分析

目的 了解陕西省咸阳城区女性人乳头瘤病毒(HPV)感染状况及基因亚型的分布.方法 采用聚合酶链反应(PCR)-反向点杂交法检测1 642例女性生殖道分泌物19种HPV基因亚型.结果 HPV总感染率为18.70%(307/1 642).19种亚型均被检出,其中高危型HPV感染268例,感染率为16.32%,低危型HPV感染39例,感染率为2.38%,二者比较差异有统计学意义(χ2=141.64,P<0.05).单一感染247例,感染率为15.04%,单一感染的感染率与多重感染比较,差异有统计学意义(χ2=93.83,P<0.05).感染高危型HPV基因型前5位者依次为HPV 52、16、58、51、53.在307例感染者中HPV 16、18感染47例(15.31%);单一型别感染247例(80.45%).结论 陕西省咸阳城区女性HPV感染率低于全国其他地区,以单一、高危型感染为主.应注重高危型HPV生殖道感染对宫颈上皮内瘤变(CIN)的病变进展及其预后评估.     

The role of papilloma virus in development of cervix precancer and cancer (a literature review)

There are now enough data confirming the causal relationship between the papilloma-virus infection and the lesion of the neck of uterus. Virtually all dysplasias contain the human papilloma virus either as an infection virion or as episome-type or integrated DNA. The chronic papilloma-virus infection of high risk is an indispensable precondition for creating a precancerous and malignant phenotypes. However, considering the human papilloma-virus infection to be the key risk factor of precancerosis and cervical carcinoma, one should not fail to take into account other impacts. The clinical infection signs result from an interaction of a number of factors, like virus type, human immune and hormonal systems, other infection types as well as human behavior (sex activity, smoking, alcohol etc.). Because of the infection transmission nature and its extensive spreading, its treatment is related with certain difficulties. Therefore, the radical treatment of infection consequences (pathologies of the neck of uterus) must be in the focus of attention, which, in the end, would ensure success in coping with the infection. The immunological methods deserve attention, however, they are still in the experimental stage.     

人类乳头状瘤病毒在结直肠癌中的感染及其分型的研究

目的研究结直肠癌与人乳头状瘤病毒（HPV）感染的相关性并分型。方法入选2004～2008年住院治疗的结直肠癌患者95例,同时收集50例结直肠腺瘤患者资料,采用分子杂交基因芯片法检测结直肠癌石蜡组织中的HPV感染情况并分型,将结直肠患者分为结肠组43例,直肠组52例;中分化组79例,中分化16例,分别比较两组间的差异。结果结直肠癌患者人乳头状瘤病毒型感染阳性者46例,阳性率为48．8％（46／95）,其中结直肠癌中感染HPV16型占所有阳性样本的97．8％（45／46）,HPV53检出为2．2％（1／46）;结直肠癌旁HPV感染阳性率为30％（15／50）,结直肠腺瘤HPV感染感染阳性率为28％（14／50）,三组差异有统计学意义（P〈0．05）;结肠组和直肠组的阳性率分别为48．8％和48．1％,两组比较无统计学差异。低分化组、中分化组阳性率分别为46．8％和56．3％,两组比较差异无统计学意义。结论结直肠癌的发生与人乳头状瘤病毒感染有关,以HPV16型为主。     

女性生殖道人乳头瘤病毒与解脲支原体感染之间的相关性研究

目的：探讨女性人乳头瘤病毒（HPV）感染情况及与解脲支原体（UU）感染之间的相关性。方法采用 PCR-反向点杂交基因分型技术对412例女性患者宫颈分泌物进行23种 HPV 亚型感染检测;将 HPV 阳性的患者设为观察组,同时随机选取50例 HPV 阴性的患者作为对照组,运用实时荧光 PCR 同时检测上述2组 UU 的感染情况。结果HPV 总的感染率为12．86％（53/412）,有16种型别被检出,感染率最高的为高危 HPV 52型。HPV 阴性对照组的 UU 阳性率为30．00％（15/50）,而 HPV阳性观察组中 UU 的阳性率为60．38％（32/53）,明显高于 HPV 阴性对照组,差异有统计学意义（P ＜0．05）。结论HPV 感染与UU 感染具有一定的相关性,在临床治疗中应予以重视。     

不同年龄段女性人乳头瘤病毒感染状况分析

目的了解不同年龄段女性人乳头瘤病毒(HPV)感染状况,为宫颈癌防治提供理论依据。方法对临床3 426例宫颈分泌物标本采用多通道实时荧光定量聚合酶链反应(PCR)仪检测8种高危型HPV DNA亚型(HPV16、18、45、31、33、52、58、67),分析不同年龄段受检者HPV感染状况。结果 3 426例标本中共检出HPV阳性583例,阳性率为17.02%。HPV感染人群分布以19~40岁组为主,以19~30岁人群中感染率最高,达到20.37%,明显高于其余的年龄组(P0.05)。在检出类型中有单一亚型感染,也有多亚型的多重感染,其中比例最高的亚型是HPV33/52/58/67(59.01%),明显高于其它HPV亚型(P0.05);居第2位和第3位的分别为HPV16(14.92%)、HPV18/45(9.43%)。结论 19~30岁是女性感染HPV的高峰期,HPV感染有年轻化趋势,但中老年女性群体中HPV感染率依然居高不下。常见的HPV感染基因型为33/52/58/67、16和18/45,提示有一定人群特点。     

肺鳞状细胞癌中人乳头瘤病毒感染的研究

目的通过观察肺鳞状细胞癌及癌旁肺组织中人乳头瘤病毒（humanpapillomavirus，HPV）的感染、分布、存在状态及与临床病理资料间的关系，探讨HPV感染在肺鳞状细胞癌发病中的作用。方法用共有引物PCR检测120例肺鳞状细胞癌及癌旁肺组织中HPV的感染率，用多重PCR对阳性标本的HPV6/11．16．18分型，用原位杂交方法观察HPV在肺鳞癌组织中的分布及存在状态。结果肺鳞癌组织HPV感染率（35％）显著高于癌旁肺组织（67％）（P＜0．01），HPV在肺鳞癌组织中可能以游离和整合两种状态存在并可在肺鳞癌组织中表达，早期（Ⅰ期）肺鳞癌HPV感染率（44.3％）显著高于进展期（Ⅱ～Ⅲ期）肺鳞癌（25．4％）（P＜0．05），重度吸烟组HPV感染率（44．7％）显著高于非吸烟组（18．2％）（P＜0．01）。结论HPV感染在肺鳞癌的发病中可能起一定作用，肺鳞癌组织中HPV感染与吸烟有关。     

妊娠期妇女感染HPV对产妇和胎儿的影响

目的分析妊娠期妇女感染人乳头瘤病毒(HPV)对产妇和胎儿的影响。方法选取116例感染人乳头瘤病毒的产妇作为实验组,另选取健康孕妇116例为对照组,分别对产妇分娩时羊水、胎盘组织及胎儿脐静脉血、口咽部分泌物与外阴分泌物中的HPV进行检测,分析产妇HPV的感染分型。随访1年,观察HPV感染对产妇和胎儿的影响。结果实验组中单型HPV感染及高危型HPV感染的患者所占比例较大,分别为66.38%和76.72%;患者宫颈肉眼形态异常炎症、细胞学检验呈鳞状上皮病变是感染HPV的危险因素;2组患者在分娩方式、产后出血及胎儿生长受限等方面比较,差异无统计学意义(P0.05);正常阴道分娩与剖宫产分娩的胎儿在羊水、胎盘组织及脐静脉血、口咽部分泌物、外阴分泌物或包皮分泌物中的HPV阳性检出率比较无显著差异(P0.05);胎儿HPV感染率为24.14%。结论单型HPV感染及高危型HPV感染是妊娠期妇女HPV感染的主要分型。     

粤东地区妇女下生殖道HPV亚型的基因芯片检测与分析

目的了解粤东地区妇女生殖道人乳头瘤病毒(HPV)感染率及型别分布情况。方法采用基因芯片技术对569例粤东地区妇女生殖道分泌物或脱落细胞进行18种高危型HPV和5种低危型HPV检测。结果569例患者中HPV感染275例,占48.3%。单一感染183例,占32.2%;双重感染67例,占11.8%;三重以上感染25例,占4.4%。275例阳性标本主要为高危型HPV16、18型,低危型HPV6、11型。结论粤东地区妇女生殖道病变患者HPV感染率较高,且以高危型HPV16感染率最高,因此,建立建全粤东地区妇女HPV监测体系,及早发现高危人群,最大限度地降低子宫颈癌对妇女生命和健康的威胁度。     

粤东地区妇女下生殖道HPV亚型的基因芯片检测与分析

目的 了解粤东地区妇女生殖道人乳头瘤病毒(HPV)感染率及型别分布情况.方法 采用基因芯片技术对569例粤东地区妇女生殖道分泌物或脱落细胞进行18种高危型HPV和5种低危型HPV检测.结果 569例患者中HPV感染275例,占48.3%.单一感染183例,占32.2%;双重感染67例,占11.8%;三重以上感染25例,占4.4%.275例阳性标本主要为高危型HPV16、18型,低危型HPV6、11型.结论 粤东地区妇女生殖道病变患者HPV感染率较高,且以高危型HPV16感染率最高,因此,建立建全粤东地区妇女HPV监测体系,及早发现高危人群,最大限度地降低子宫颈癌对妇女生命和健康的威胁度.     

RT-PCR-based evaluation of the activity of human papilloma virus infection in relapsing papillomatosis of the larynx

RT-PCR-based examination of papilloma samples obtained from patients with relapsing papillomatosis of the larynx showed an incidence rate of human papilloma virus (HPV) amounting to 89%. The viral load level of the studied samples, when measured by concurrent RT-PCR HPV, differed by more than 130 times. It made, in the untreated patient, 1.2 x 10(9) hormonal equivalents/ml, i.e. 13-fold higher versus the patient who received pathogenetic therapy. Thus, the approach in question provides for a possibility to monitor the activity of papilloma viral infection and to evaluate the efficiency of different variations of pathogenetic therapy because the "classic" variant of PCR-detection is not informative in the discussed case.