口腔癌患者外周血白细胞介素2和可溶性白细胞介素2受体水平的临床研究

对２９例口腔癌患者外周血白细胞介素２（ＩＬ－２）活性和可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）含量进行检测，并对其中１６例术后患者外周血ＩＬ－２和ｓＩＬ－２Ｒ水平进行检测。结果表明：口腔癌患者ＩＬ－２活性明显低于正常对照组（Ｐ＜０．００１）。ｓＩＬ－２Ｒ水平明显高于正常对照组（Ｐ＜０．００１）。手术治疗后ＩＬ－２活性明显回升，ｓＩＬ－２Ｒ水平则显著下降（Ｐ＜０．０１）。研究还发现ＩＬ－２及ｓＩＬ－２Ｒ水平与肿瘤临床分期密切相关（Ｐ＜０．０５）。口腔癌患者外周血ＩＬ－２和ｓＩＬ－２Ｒ水平的变化可能与肿瘤大小、治疗效果和复发、预后密切相关。外周血ＩＬ－２和ｓＩＬ－２Ｒ水平的检测对口腔癌的诊断、病情的判断及预后监测具有一定的临床意义。     

口腔癌患者外周血白细胞介素2和可溶性白细胞介素2受体水平…

对２９例口腔癌患者外周血白细胞介素２（ＩＬ－２）活性和可溶性白细胞介素２受体（ｓＩｌ－２Ｒ）含量进行检测，并对其中１６例术后患者外周血ＩＬ－２和ｓＩＬ－２Ｒ水平进行检测，结果表明：口腔癌患者ＩＬ－２活性明显低于正常对照组（Ｐ〈０．００１）。ｓＩＬ－２Ｒ水平明显高于正常对照组（Ｐ〈０．００１）。手术治疗后ＩＬ－２活性明显回升，ｓＩＬ－２Ｒ水平则显著下降（Ｐ〈０．０１）。研究还发现ＩＬ－２及ｓＩＬ－２     

口腔颌面部肿瘤患者血清可溶性白细胞介素—2受体水平的研究

作者用双抗体夹心法酶联免疫吸附试验（ＥＬＩＳＡ法）检测了４６例颌面部肿瘤患者血清可溶性白细胞介素－２受体（ＳＩＬ－２Ｒ）水平，并与２５例正常健康人进行对照比较，结果显示，口腔颌面部恶性肿瘤患者血清ＳＩＬ－２Ｒ水平显著高于颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平（Ｐ〈０．０１）及正常健康人血清ＳＩＬ－２Ｒ水平（Ｐ〈０．０１），而颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平与正常健康人血清ＳＩＬ－２Ｒ水平之间     

牙周病患者牙龈组织前列腺素E_2、血栓素B_2、6－酮－前列腺素F_（1α）水平的研究

采用ＲＩＡ对１１例健康牙龈组织（Ｈ）、１１名龈炎（Ｇ）患者的１７个牙、２６名成人牙周炎（ＡＰ）患者３６个牙和１４名青少年牙周炎（ＪＰ）患者的２０个牙的牙龈组织同时测定ＰＧＥ_２、ＴＸＢ_２、６－Ｋ－ＰＧＦ_（１α）的含量。结果以ＰＧＥ_２最多，ＴＸＢ_２次之，６－Ｋ－ＰＧＦ_（１α）最少；３种前列腺素（ＰＧｓ）均随病变严重程度而增加，除Ｇ组ＴＸＢ_２和６－Ｋ－ＰＧＦ_（１α）与Ｈ组相差显著（Ｐ＜０．０５）外，其余各病变组与Ｈ组比，３种ＰＧｓ均相差非常显著（Ｐ＜０．０１），ＡＰ组和ＪＰ组比Ｇ组也有非常显著升高（Ｐ＜０．００１），而ＡＰ和ＪＰ组间相差不显著（Ｐ＞０．０５），表明３种ＰＧｓ在牙周病发病中具有重要作用。     

胰岛素对人牙周膜细胞碱性磷酸酶活性和总蛋白含量的影响

目的：探讨胰岛素作用下，牙周膜（ＰＤＬ）细胞碱性磷酸酶（ＡＬＰ）活性和总蛋白含量的变化。方法：细胞生物学法、酶动力学法和考马斯亮蓝法。结果：胰岛素浓度超过１０－４Ｕ／ｍｌ时，明显增加ＰＤＬ细胞的ＡＬＰ活性和总蛋白含量（Ｐ＜０．０１）。其中，在１０－４～１０－１Ｕ／ｍｌ之间，效应随浓度增加而增加，超过１０－１Ｕ／ｍｌ效应趋于稳定，此时效应最大，ＡＬＰ活性和总蛋白含量分别比对照组增加１．４倍和１．２倍。结论：胰岛素明显促进ＰＤＬ细胞分化及蛋白合成功能。胰岛素作为骨代谢的重要调节因子，可能对牙周组织的再生修复有调节作用。     

头颈癌患者白细胞介素2与T淋巴细胞亚群的检测

对３５例头颈癌患者手术前后血清白细胞介素２（ＩＬ－２）水平及Ｔ淋巴细胞亚群进行检测发现：头颈癌患者手术前ＣＤ８＋显著增高，ＣＤ４＋／ＣＤ８＋比值及血清ＩＬ－２水平明显低于正常人（Ｐ＜０．０１），且三者与肿瘤临床分期有关，而ＣＤ４＋无明显差异（Ｐ＞０．０５）；手术后ＣＤ８＋显著下降（Ｐ＜０．０１），ＣＤ４＋／ＣＤ８＋比值与血清ＩＬ－２水平呈明显回升，与术前相比有显著性差异（Ｐ＜０．０５）。结果提示：头颈癌患者存在着明显的细胞免疫受损，外周血Ｔ淋巴细胞亚群及血清ＩＬ－２水平能反应机体抗肿瘤免疫水平的高低，并可作为监测病情发展及制定治疗方案的免疫学客观指标之一。     

口腔颌面部肿瘤患者血清可溶性白细胞介素─2受体水平的研究

作者用双抗体夹心法酶联免疫吸附试验（ＥＬＩＳＡ法）检测了４６例颌面部肿瘤患者血清可溶性白细胞介素－２受体（ＳＩＬ－２Ｒ）水平、并与２５例正常健康人进行对照比较。结果显示：口腔颌面部恶性肿瘤患者血清ＳＩＬ－２Ｒ水平显著高于颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平（Ｐ＜０．０ｌ）及正常健康人血清ＳＩＬ－２Ｒ水平（Ｐ＜０．０ｌ），而颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平与正常健康人血清ＳＩＬ－２Ｒ水平之间无显著性差异（Ｐ＞０．０５）。本研究表明、血清ＳＩＬ－２Ｒ有可能作为颌面部恶性肿瘤的诊断、疗效考核及预后判断的一个辅助监测指标。     

口腔颌面部肿瘤患者T淋巴细胞亚群及细胞因子检测的临床意义

本文对４０例口腔颌面部恶性肿瘤及２０例良性肿瘤患者同时进行外周血Ｔ淋巴细胞亚群、ｓＩＬ－２Ｒ及ＴＮＦ三项指标检测，结果表明，１．良性、恶性患者均有ＣＤ３值明显下降（Ｐ＜０．０５、ｐ＜０．０ｌ），ｃＤ４／ＣＤ８比值明显下降（ｐ＜０．００ｌ），ＣＤ８值明显上升（Ｐ＜０．００１），并且良性高于恶性患者（ｐ＜０．０５），ｃＤ４值恶性患者有下降趋势（ｐ＞０．０５）。２．恶性患者ｓＩＬ－２Ｒ水平明显高于正常人（ｐ＜０．０１），也高于良性患者（ｐ＜０。０５）。３．ＴＮＦ水平三组尚无明显差异（Ｐ＞０。０５）。４．三项检验指标与恶性肿瘤临床分期有一定相关趋势。提示：Ｔ淋巴细胞亚群、ｓＩＬ－２Ｒ检测对口腔颌面部恶性肿瘤诊断及预后判断具有一定参考价值，ＴＮＦ检测的临床意义尚须进一步研究。     

人牙髓中6—酮—前列腺素F1α,血栓素B2浓度的放射免疫 …

目的：观察正常、龋病、慢性牙髓炎、慢性牙髓炎急性发作患者的牙髓中６－酮－前列腺素Ｆ１α、血栓素Ｂ２的含量，揭示ＰＧＩ２和ＴＸＡ２的作用。方法：放射免疫检测法检测。结果：龋病、慢性牙髓炎、慢性牙髓炎急性发作三组中６－酮－ＰＧＦ１α和ＴＸＢ２水平明显高于正常组（Ｐ〈０．０１）。慢性牙央炎组中ＴＸＢ２水平高于龋病组（Ｐ〈０．０１），慢性牙髓炎急性发作组中６－酮－ＰＧＦ１α水平明显高于其它组（Ｐ〈０．０１     

牙周炎患者红细胞免疫状态的初步分析

目的：通过对牙周炎患者外周血红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）和红细胞免疫复合物花环率（ＲＢＣ－ＩＣＲ）的检测,观察青少年牙周炎（ＪＰ）、快速进展型牙周炎（ＲＰＰ）、成人牙周炎（ＡＰ）的红细胞免疫功能状况。方法：采用红细胞酵母菌花环试验方法。结果：ＪＰ、ＲＰＰ患者红细胞免疫粘附力明显低于正常人。ＪＰ、ＲＰＰ患者ＲＢＣ－Ｃ３ｂＲＲ分别为（１０．０３±０．９２）％、（１２．２０±０．９４）％,与对照组（１５．９０±１．７７）％有显著差异（Ｐ＜０．００１,Ｐ＜０．０１）;ＲＢＣ－ＩＣＲ分别为（２７．８９±１．７７）％、（２７．０１±１．７９）％,与对照组（３０．６４±３７）％有显著性差异（Ｐ＜０．０１）;ＡＰ患者ＲＢＣ－Ｃ３ｂＲＲ为（１６．０２±１．２５）％、ＲＢＣ－ＩＣＲ为（３０．０４±１．７３）％,与对照组无显著性差异（Ｐ＞０．０５）。结论：提示ＪＰ、ＲＰＰ的发病机理可能与宿主的红细胞免疫功能低下有关。     

大鼠实验性根尖周病的酶组织化学研究

目的：本研究旨在通过石蜡包埋的酶组织化学染色法，观察大鼠实验性根尖周病病损区成骨细胞、破骨细胞的活动情况。方法：将大鼠右下颌第一磨牙开髓后暴露口腔2—28d，诱导根尖周病损形成；对其进行心内固定后，将含牙和牙槽骨的标本经KDTA脱钙，采用石蜡包埋法制作切片，进行酸性磷酸酶和碱性磷酸酶酶组织化学染色。结果：石蜡切片经酶组织化学染色后，可清晰辨别出酶阳性的成骨细胞和破骨细胞。随根尖周病的发展，成骨细胞和破骨细胞在数量上呈一定规律性变化。结论：石蜡切片的酶组织化学法可用于研究根尖周病损区骨细胞的行为变化。     

Ultrastructural localization of alkaline and acid phosphatase activities in dental plaque

Ultrastructural cytohistochemical techniques showed presence of acid and alkaline phosphatases in dental plaque. Both phosphatases had intra- and extramicrobial localization. In the extracellular matrix, phosphatases were associated with small vesicles of bacterial origin, or were freely scattered in the matrix without apparent connection with microbial structures. Intracellularly, alkaline (AlkP) and acid (AcP) phosphatases were observed in Gram-negative and Gram-positive bacteria, showing a different localization. The AlkP was mainly located in the periplasmic space, while AcP had a double preferential localization: along the outer surface of the cell wall and in the periplasmic space. Less frequently an intracellular phosphatase reaction was seen in the cytoplasm.     

低碱性磷酸酯酶症及其在口腔中的表现

低碱性磷酸酯酶症(HP)又称低磷酸酶血症,是一种罕见的遗传性全身系统疾病,主要特征为骨骼和牙齿的矿化不全,血清及骨组织中碱性磷酸酶活性降低.据统计该病的严重型发病率为1/100000,而轻型发病率相对较高,其临床表现有很大的变异性,有的仅表现为年轻恒上前牙过早脱落,有的会出现严重的全身性骨骼形成不良,甚至导致新生儿死亡...     

酸性和碱性磷酸酶检测牙槽骨破坏与重建初探

目的 :分析动物模型牙周组织中成骨和破骨细胞数 ,ACP和 AKP反应性 .方法 :豚鼠急性牙周创伤48h和狗人工二壁骨袋术后 1月 ,采用酶细胞化学和常规 HE染色检测组织切片进行 t检验 .结果 :急性牙周创伤组和人工二壁骨袋组中成骨细胞和破骨细胞数增加 ,ACP和 AKP反应增强 p<0 .0 5 .结论 :AKP参与成骨 ,ACP与成骨细胞和破骨细胞功能有关 ,ACP和 AKP的活性可检测牙槽骨的吸收和重建。     

Activity of alkaline and acid phosphatases in dental epithelial cells and enameloid during odontogenesis in two teleost fish,Oreochromis niloticus and Tilapia buttikoferi

The dental epithelial cells and enameloid at the stages of enameloid matrix formation, mineralization and maturation in the teleosts Oreochromis niloticus and Tilapia buttikoferi were investigated by means of enzyme histochemistry in order to identify their functions associated with the structural modification. No marked enzyme activities were found in the inner dental epithelial cells in the stage of enameloid matrix formation, although the outer dental epithelial cells often exhibited moderate alkaline phosphatase (ALPase) activity. In the stages of enameloid mineralization and maturation, the inner dental epithelial cells, which possessed a ruffled border at the distal ends, showed intense ALPase activity at their lateral and proximal cell membranes. At the same time, many acid phosphatase (ACPase)-positive vesicles and granules were localized at the distal cytoplasm of the inner dental epithelial cells. The outer dental epithelial cells, which contained well-developed labyrinthine canalicular spaces, showed neither marked ALPase nor ACPase activity. It is postulated that the dental epithelial cells in these two teleosts are mainly involved in the removal of the organic matrix from the enameloid, and in material transport to the enameloid during the later half of odontogenesis.     

Inhibitory effects of green tea catechins on protein tyrosine phosphatase in Prevotella intermedia

Members of the Prevotella intermedia group possess protein tyrosine phosphatase (PTPase). The purpose of this study was to investigate the effects of catechin derivatives from Japanese green tea on the activity of PTPase in P. intermedia and related organisms. Multilocus enzyme electrophoresis of alkaline phosphatase derived from P. intermedia, Prevotella nigrescens, Prevotella pallens and Porphyromonas gingivalis revealed a species-specific migration pattern. Among the tea catechin derivatives tested, (-)-epigallocatechin gallate (EGCg), similar to orthovanadate, a specific inhibitor for PTPase, was effective in inhibiting the PTPase activity in P. intermedia at 0.5 microm, and related species at 5 microm. The results suggested that the inhibitory effect observed is due to the presence of galloyl moiety in the structure. In contrast, neither the green tea catechins nor orthovanadate inhibited the phosphatase activity in P. gingivalis, suggesting that this organism possessed a different family of alkaline phosphatase.     

Temporal and local appearance of alkaline phosphatase activity in early stages of guided bone regeneration. A descriptive histochemical study in humans

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters and it seems to be a prerequisite for normal skeletal mineralization. Also, ALP is the most widely recognized marker of osteoblast phenotypes. By a tissue regenerative technique called Guided Bone Regeneration (GBR), it is possible nowadays to regenerate small bony defects. The aim of the present study was to investigate early events in bone healing and neogenesis by studying histochemically the temporal and local appearance of the marker Alkaline Phosphatase (ALP) in a GBR model system. Nine healthy volunteers (5 males, 4 females, mean age 31.7 years) participated in the experiment. After raising a mucoperiosteal flap from the mandibular second molar to the retromolar area in each volunteer, a hollow titanium test cylinder was placed into a congruent bony bed and the coronal end of the cylinder was closed with an ePTFE-membrane. Then the flap was adapted and sutured to obtain primary wound closure. After 2, 7 and 12 weeks, the regenerated tissue within the cylinders was harvested. Histologically, ALP activity was observed associated with the osteoid seams in the very basal part of the regenerate where new bone trabeculae were in the process of being formed. More coronally, large round cells seemed to secrete an ALP-positive substance since in the center of such cell clusters strong ALP activity located extracellularly was detected. In the present experiment, ALP seemed to have been an early sign of osteoblast secretion of a matrix which subsequently was determined to become osteoid. ALP activity was never seen isolated within connective tissue and away from bone. This is an indication that its source is linked to existing bone. The present study has documented for the first time the appearance of ALP activity in guided bone regenerations in humans. It has revealed that: 1) Osteogenesis in guided bone regeneration is preceded by localized, marked expression of ALP in an organized connective tissue environment. 2) Bone neogenesis is an early event in this experimental setup and may be detected already 2 weeks after wounding. 3) Expression of ALP and subsequent bone neogenesis is originating from and topographically linked to pre-existing bone structures.     

An enzyme histochemical study of dental plaque and calculus

The following investigation was undertaken to study the location in the dental plaque and calculus of certain enzyme activities and to compare the patterns obtained with those of the normal hard tissue formation.

Supragingival and subgingival calculus attached to the root surfaces of 30 extracted teeth was studied. The root with its deposits was frozen rapidly in a mixture of hexane and solid CO₂ (-75°C). From the frozen block, sections were cut and incubated for histochemical demonstration of lactate dehydrogena-se, alkaline phosphatase and acid phosphatase.

The plaque seemed to be stratified with regard to enzyme activity. Three different layers could be identified.

In the basal layer, approximately 100 um thick, enzyme activity was low. Lactate dehydrogenase activity could be identified in some sections, but no phosphatase activity.

In the middle layer lactate dehydrogenase. alkaline phosphatase and acid phosphatase activities were found in most of the sections. The superficial layer usually showed lactate dehydrogenase but not always acid or alkaline phosphatase activities. The results of the present investigation may suggest that the mineralization of the dental plaque is not only a passive mineralization of dead bacteria, but also an active process promoted by enzymes in the covering bacterial layers.     

雷尼酸锶对实验性牙周炎大鼠牙槽骨改建的影响

目的:通过观察雷尼酸锶对大鼠实验性牙周炎治疗后牙槽骨组织中酸性磷酸酶及碱性磷酸酶的变化,评估雷尼酸锶对实验性牙周炎的治疗作用,为应用雷尼酸锶治疗牙周炎提供理论依据和实验参考.方法:选取50只3月龄雄性SD大鼠,随机分为5组:正常组(A组)、牙周炎组(B组)、牙周炎局部治疗组(C组)、牙周炎雷尼酸锶治疗组(D组)、牙周炎...     

取样方法的比较及其对龈沟液酶水平的影响

目的观察重复取样后两次样本的碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）水平的变化，比较袋口和袋内两种取样方法。方法分别用袋口和袋内法间隔２０ｍｉｎ重复取龈沟液（ｇｉｎｇｉｖａｌｃｒｅｖｉｃｕ－ｌａｒｆｌｕｉｄ，ＧＣＦ）样本，并测定其中的ＡＬＰ水平。结果两种方法取样示，２０ｍｉｎ后的ＡＬＰ水平与第１次相比差异无显著性（Ｐ＞０．０５）；袋内法获得的ＡＬＰ水平明显高于袋口法（Ｐ＜０．００１）；重复取样后，多数位点ＡＬＰ水平不能完全恢复，但袋内法略优于袋口法，用酶总量表达略优于用浓度表达。结论重复取样的间隔期宜超过２０ｍｉｎ；建议用袋内法取样测定ＡＬＰ水平并以酶总量表示为佳。