健脾消积颗粒对脾虚大鼠胃液、胃酸的分泌及胃蛋白酶活性的影响

目的:探讨健脾消积颗粒对脾虚大鼠胃液、胃酸分泌及胃蛋白酶活性的影响。方法:用大黄造成大鼠脾虚模型后,再灌服健脾消积颗粒药液,于末次灌胃后1 h,测定大鼠胃液、胃酸分泌量、胃蛋白酶活性。结果:健脾消积颗粒的胃液、胃酸分泌量和胃蛋白酶的活性与模型组比较,差异具有统计学意义(P<0.05或P<0.01)。结论:健脾消积颗粒对脾虚大鼠的健脾作用机制之一与其能使脾虚大鼠的胃液、胃酸分泌增加、胃蛋白酶活性提高有关。     

肠安颗粒剂对鼠胃肠功能的影响

目的;研究肠安颗粒剂对小鼠及大鼠胃肠运动及胃液分泌功能的影响.方法;采用清醒状态下灌胃给药,测定给药后小鼠小肠内炭末前沿至幽门的距离占小肠全长、大鼠结肠内炭末前沿至回盲瓣的距离占结肠全长的百分比,小鼠胃内苯酚红的残留量、大鼠胃液与胃酸的分泌量和胃蛋白酶的活性.苯酚红含量测定采用紫外分光光度法,胃蛋白酶的活性采用Mett法.结果;肠安颗粒剂可增大小鼠小肠内炭末前沿至幽门的距离占小肠全长和大鼠结肠内炭末前沿至回盲瓣的距离占结肠全长的百分比,增加大鼠胃液、胃酸的分泌量以及增强胃蛋白酶的活性.可减少用药后30 min小鼠胃内苯酚红的残留量. 结论;肠安颗粒剂具有促进胃、小肠和大肠运动和胃液分泌的功能.     

吲哚美辛-PEG 6000滴丸的研究

以聚乙二醇6000(简称PEG 6000)为载体制成吲哚美辛滴丸。测得滴丸的溶解度比原药吲哚美辛增大一倍多,剂量减半时,滴丸对大鼠胃的刺激性显著降低,且仍有抑制基础胃酸分泌的作用。     

右旋雷贝拉唑钠对大鼠反流性食管炎模型的作用研究

目的考察右旋雷贝拉唑钠对大鼠反流性食管炎的改善作用。方法采用幽门结扎致大鼠单纯反流性食管炎模型为实验对象,以食管炎指数和抑制率、胃酸总分泌量、p H值及胃酸分泌抑制率、胃液胃蛋白酶活力等为指标,全面考察右旋雷贝拉唑钠对大鼠反流性食管炎的改善作用。结果右旋雷贝拉唑钠(4、2、1 mg·kg-1,iv),能明显降低反流性食管炎模型大鼠食管炎指数,增加胃液p H,减少胃酸总分泌量和降低胃蛋白酶活性。结论右旋雷贝拉唑钠对反流性食管炎模型大鼠有明显的改善作用。     

越鞠丸对大鼠胃酸胃蛋白酶的影响

目的:观察越鞠丸对大鼠胃酸、胃蛋白酶的影响。方法:将SD大鼠50只随机分为正常组、空白对照组、越鞠丸低剂量组、中等剂量组和高剂量组,每组10只。末次灌胃后收集胃液,测定各组大鼠胃液胃酸、胃蛋白酶浓度和胃蛋白酶活力的变化。结果:各实验组胃酸、胃蛋白酶浓度和胃蛋白酶活力均较正常组增加(P0.05),同时胃蛋白酶浓度和胃蛋白酶活力在低剂量组和高剂量组之间比较有显著差异(P0.05)。结论:越鞠丸通过提高胃液酸度、胃蛋白酶浓度及胃蛋白酶活力发挥行气解郁的生理功效,并且与高剂量相比,低剂量使用效力相当。     

仁和正胃胶囊对胃液分泌功能影响的实验研究

目的研究正胃胶囊对胃液分泌的影响。方法制备正常大鼠胃液分泌及组胺刺激大鼠胃液分泌2个模型,正胃胶囊与对照组中药制剂(胃康灵胶囊、胃泰颗粒)、西药制剂(维U颠茄铝胶囊、铝碳酸镁片)比较,测定正胃胶囊的制酸止痛作用。结果正胃胶囊能显著抑制胃蛋白酶活力,对胃液的pH值有明显升高作用;对组胺刺激的胃液分泌量增加有明显的抑制作用;对正常大鼠胃液分泌量仅有轻度抑制作用。结论正胃胶囊在抑制胃蛋白酶活性、降低胃液pH值、抑制胃液过量分泌等有明显的临床应用优势。     

小儿增食灵合剂对小儿厌食症动物模型胃蛋白酶活性和胃酸分泌量的影响

目的：研究小儿增食灵合剂对实验性幼龄大鼠消化功能的影响。方法：采用病因模拟法建立小儿厌食症动物模型，测定模型动物胃蛋白酶的活性及胃酸含量。结果：小儿增食灵合剂可使胃游离酸和胃总酸分泌量增加，能提高大鼠胃蛋白酶活力，与空白对照组和模型对照组比较，均有统计学意义（P〈0．05或P〈0．01）。结论：小儿增食灵合剂治疗小儿厌食症的作用机制可能与其有效地增加胃游离酸及总酸度、提高胃蛋白酶的活性，从而达到促进消化的作用有关。     

胃散对胃溃疡模型大鼠胃黏膜保护作用的研究

目的 研究胃散对胃溃疡大鼠胃黏膜的保护作用。方法 采用乙酸烧灼型胃溃疡模型、幽门结扎型胃溃疡模型、乙醇损伤型胃溃疡模型,检测溃疡指数、胃酸总酸度、胃酸分泌速度和胃蛋白酶活性,综合考察胃散对胃溃疡模型大鼠胃黏膜的保护作用。结果 胃散在1、0.5、0.25 g/kg剂量下,对乙酸烧灼型胃溃疡有非常显著的促愈合作用;对幽门结扎型胃溃疡的形成有显著的抑制作用;对乙醇损伤的胃黏膜也有显著的保护作用;对胃液总酸度、胃酸分泌速度和胃蛋白酶活性有明显抑制作用,显著增加胃液分泌量。结论 胃散具有增加胃液分泌、抑制胃酸分泌、抑制胃蛋白酶活性、保护胃黏膜和防止胃溃疡的作用。     

刺梨根煎液防治慢性胃溃疡的实验研究

目的：为研究刺梨根治疗慢性胃溃疡的药用价值及作用机制。方法：采用乙酸制备大鼠慢性胃溃疡模型。以刺梨根煎液作为研究药物,生理盐水作为对照,观察刺梨根煎液治疗大鼠慢性胃溃疡的疗效。同时检测了大鼠胃粘膜MDA含量、SOD活力、胃粘膜血流量及胃酸分泌量。结果：刺梨根煎液能够使溃疡面积明显缩小,胃粘膜血流量增加,胃粘膜SOD活力升高,MDA含量降低,但不影响胃酸分泌。结论：刺梨根可促进胃溃汤疡愈合。     

小儿健脾膏对功能性消化不良大鼠胃肌电、胃分泌及脑肠肽的影响

目的 探讨小儿健脾膏(Xiao’er Jianpi cream,XEJPC)促胃肠动力治疗功能性消化不良(functional dyspesia,FD)的作用机制。方法 通过不规则饮食并饮用酸化水加慢性疲劳刺激法建立大鼠FD模型,观察大鼠体质量、摄食与饮水等一般状况,生物信号采集系统测定大鼠胃肌电,结扎幽门法测定胃酸分泌速度与胃蛋白酶活性,酶联免疫法检测血清胃动素(motilin,MTL)、胃泌素(gastrin,GAS)及P物质(substance P,SP)的含量。结果 XEJPC口服与外敷均能明显增加模型大鼠的摄食量、饮水量与体质量;增强胃肌收缩节律与强度、显著减小FD大鼠胃酸分泌速度、增强胃蛋白酶活性、提高MTL、GAS及SP在血清中含量。结论 XEJPC促胃肠动力治疗FD的药理作用与其提高胃肌收缩节律与强度、减小FD胃酸分泌、增强胃蛋白酶活性、促进MTL、GAS及SP的分泌等有关。     

胃复春片对功能性消化不良大鼠胃肠功能的影响

目的 考察胃复春片对胃肠动力障碍的改善作用。方法 建立功能性消化不良（functional dyspepsia,FD）大鼠模型;以FD大鼠体质量、进食量、饮水量、胃平滑肌电活动情况、胃液分泌量、胃蛋白酶活性、肝脏与脾脏质量、胃病理情况等考察胃复春的药效。结果 模型组FD大鼠胃平滑肌电活动明显紊乱,体质量、进食量、饮水量明显减少、胃蛋白酶活性明显降低;经胃复春治疗后,上述现象明显缓解,大鼠体质量、进食量、饮水量明显增加,胃蛋白酶活性明显升高。结论 胃复春能改善FD大鼠的一般状况,对FD大鼠胃酸过多有抑制作用,能提高模型大鼠的胃蛋白酶活性。     

Gastric pharmacological activities of tripotassium dicitrato bismuthate in rats and dogs

The effects of tripotassium dicitrato bismuthate (TDB) on gastric acid, pepsin and mucoprotein secretion in rats and on hydrochloric-peptic secretion and plasma gastrin levels in dogs were investigated. In Shay rats, TDB did not affect acid secretion but significantly lowered pepsin concentration and increased the amount of bound mucoproteins. In addition, gastric mucosal lesions were significantly prevented by the drug. In dogs, chronically fitted with both gastric fistulae and Heidenhain pouches, acid secretion and plasma gastrin levels stimulated by a meat meal were unaffected by TDB, while pepsin concentration and pepsin output were significantly decreased. On the basis of these results, the antiulcer activity of TDB appears to be ascribed to the protection of the gastric mucosa through an increase in mucoprotein synthesis and a decrease of pepsin activity.     

Effect of 40749 RP on basal and sham feeding stimulated gastric secretion in man

Summary 40749 RP, a pyridyl-2-tetrahydrothiophene derivative, is known to be a potent inhibitor of the gastric acid response to pentagastrin, betazole and a meal.In 6 healthy young volunteers, a single oral dose of 2 mg · kg^–1 greatly reduced the gastric acid secretory response to sham-feeding. By contrast, neither gastric pepsin nor the plasma PP response were altered by the drug. No change was observed in plasma gastrin, motilin, VIP or somatostatin concentrations.The results show that 40749 RP is also active on the pure vagus-stimulated gastric acid secretion. The lack of effect upon gastric pepsin and plasma PP suggests that 40749 RP is not likely to act on the basolateral cholinergic receptor and that it affects further cellular steps involved in hydrogen ion secretion.     

莨菪类药物对大鼠胃粘膜损伤的影响

本文观察了莨菪类药物(东茛菪碱、樟柳碱、山茛菪碱)对大鼠三种实验性胃溃疡的影响,并对其作用机制进行了初步的探讨。结果表明:莨菪类药物有对抗大鼠应激性胃溃疡、药物性胃溃疡及慢性胃溃疡的作用,且有量效依赖关系;对胃液和血液等各项生化指标分析表明,这类药物有抑制胃酸分泌、降低胃蛋白酶活性、增强胃粘液屏障,提高血清胃泌素浓度的作用。提示这些结果可能与其抗溃疡效应有关。     

The relationship between blood gastrin levels and gastric secretion in conscious dogs

1 In conscious dogs with gastric fistulae and Heidenhain pouches, acid and pepsin secretion, immunoreactive plasma pancreatic polypeptide (PP) and gastrin were measured following intravenously administered pentagastrin, cholinomimetics, and intragastric administration of milk. 2 Pentagastrin did not raise endogenous plasma gastrin. There was a significant positive dose-response relationship between pentagastrin and acid and fistula pepsin secretions, but not between plasma gastrin of endogenous origin and gastric secretion. 3 Carbachol raised plasma gastrin immunoreactivity; but in no instance was there a significant relationship between gastric secretion and plasma gastrin immunoreactivity. Gastric acid secretion faded, but plasma gastrin concentrations did not. 4 PP plasma immunoreactivity was elevated by methacholine and carbachol. Its levels correlated significantly with gastric acid secretion. Pentagastrin did not raise PP and its levels did not, therefore, correlate with gastric acid secretion. 5 Intragastric milk raised plasma gastrin immunoreactivity; but the acid secretion per pg of plasma gastrin was much smaller than with the cholinomimetics. Ganglionic blocking agents depressed both plasma gastrin and acid and pepsin secretion. 6 The results suggest that cholinomimetics sensitize parietal cells to the stimulating action of gastrin.     

胃复春胶囊通过抑制AQP3调节炎症性水液代谢障碍改善大鼠急性胃溃疡

目的 通过观察胃复春胶囊对大鼠胃溃疡组织炎症因子和水通道蛋白(aquaporins,AQPs)表达的影响,从水液代谢角度阐释胃复春胶囊对胃溃疡的干预作用,为临床上胃复春胶囊用于胃溃疡的防治提供实验和理论依据。方法 取体质量为190~210 g的雄性SD大鼠40只,按照体质量随机分为假手术组(生理盐水)、模型组(生理盐水)、阳性对照组(雷尼替丁30 mg·kg^-1)和胃复春高、低剂量组(1 000,500 mg·kg^-1)5组。造模前,各组大鼠分别以口服灌胃方式给予相应剂量的药物。连续给药3 d后行乙酸致大鼠胃溃疡模型手术。继续给药5 d后处理大鼠,解剖收集胃液,检测胃液量和胃蛋白酶活性。取胃组织用于大体和病理组织学观察。检测胃组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)和炎症因子的水平,RT-PCR法检测胃组织中AQPs mRNA的表达情况,免疫荧光法检测胃组织中AQP3蛋白的表达情况。分离各组大鼠胃壁细胞,共聚焦显微镜进一步确认胃壁细胞中AQP3的表达水平。结果 胃溃疡模型组大鼠胃溃疡、糜烂明显,伴有出血点;胃复春高、低剂量组大鼠胃黏膜溃疡、糜烂有不同程度的改善。病理组织学可见,模型组黏膜水肿糜烂,炎性细胞浸润,固有层腺体破坏,间质充血等典型胃溃疡症状;胃复春不同剂量组对胃溃疡特征性病变有显著的改善作用。模型组胃蛋白酶活性显著升高,胃复春高、低剂量组和阳性对照组胃蛋白酶活性均下降。此外,胃复春胶囊可显著减低胃溃疡大鼠胃液分泌量,抑制胃蛋白酶活性,提高胃抗氧化酶活性,降低血清炎症因子水平。通过RT-PCR和免疫荧光实验可见,胃复春胶囊对胃溃疡组织AQP3 mRNA和蛋白水平的表达均有显著调控作用,进一步分离胃壁细胞,证实胃复春胶囊对胃壁细胞中的AQP3表达有显著抑制作用。结论 胃复春胶囊能显著改善大鼠胃溃疡症状,提高机体抗氧化水平,调节炎性水液代谢障碍,其作用机制可能与抑制胃壁细胞中AQP3的表达有关。     

沙棘糖浆对食积症模型小鼠的消食化滞作用

目的 研究沙棘糖浆的消食化滞作用,初步探讨其作用机制。方法 除空白组外,其余各组饲喂定制的高热量高蛋白饲料及牛奶建立食积症小鼠模型。造模成功后按体重将小鼠随机分成5组,分别为模型对照组,阳性对照组,沙棘糖浆高、中、低剂量组。各组分别给予相应药物灌胃,每日1次,连续7天,期间统计排便粒数,称重粪便。末次给药后,收集胃液上清,测定胃液pH值,试剂盒检测胃蛋白酶（pepsin）活性,胃泌素（GAS）、胃动素(MTL)水平。结果 沙棘糖浆高剂量显著增加小鼠的排便粒数（与模型对照组相比,P＜0.05）,且有增加排便重量的趋势。沙棘糖浆高剂量可降低胃液pH值,与模型对照组相比有显著性差异（P＜0.05）。与模型对照组比较,沙棘糖浆中剂量组胃液中胃动素水平显著升高,有显著性差异(P＜0.05);各剂量组中胃泌素水平和胃蛋白酶活性无显著性差异（P＞0.05）。结论 沙棘糖浆具有消食化滞作用,并可通过提高胃液胃动素水平来促进胃肠运动。     

四种多糖抗溃疡作用的研究

糊精、人参果胶、肝素和硫酸软骨素A对四种大鼠实验性胃溃疡(消炎痛型、应激型、幽门结扎型及醋酸型)均有不同程度的抑制作用,前两种植物多糖抗溃疡作用比后两种酸性粘多糖强。其中以糊精的抗溃疡作用最显著。多糖的抗溃疡作用可能与其降低胃酸和抑制胃蛋白酶活性有关。     

Effect of Tityus serrulatus scorpion toxin on serum gastrin levels in anaesthetized rat

Scorpion toxin induces gastric secretion of acid and pepsin in rats. These effects seem to be mediated by the release of acetylcholine and histamine. However, the role of gastrin in the scorpion-toxin-induced gastric secretion is unknown. We describe the effects of the T₁ fraction purified from Tityus serrulatus scorpion venom on serum and on antral tissue gastrin levels in anaesthetized rats. Gastrin levels in serum and in the antral mucosa were measured before and at intervals 5, 15, 30, 60, 90 up to 120 min after the intravenous injection of saline or the T₁ fraction of scorpion venom (0.25 mg/kg) into anaesthetized rats. Antral G-cells were submitted to immunocytochemistry and electron microscopy. The data on gastrin were correlated with the gastric juice volume, and the acid and pepsin output increases induced by toxin. Scorpion toxin induced a significant increase in volume, acid output and pepsin output of gastric juice and gastrin serum levels 15–60 min after injection. Simultaneous measurements of antral gastrin levels did not show significant effects. The number of dense, intermediate and empty granules per μm² in the cytoplasm of antral G-cells was not significantly changed 60 min after saline or toxin injection. Scorpion toxin significantly increased serum gastrin; levels in rats.