依达拉奉联合神经节苷脂对急性脑梗死患者血清炎性因子及神经功能的影响

目的探讨依达拉奉联合神经节苷脂对急性脑梗死患者血清炎性因子及神经功能的影响。方法选择固始县中医院收治的76例急性脑梗死患者作为研究对象,随机分为观察组(38例)与对照组(38例)。对照组采用依达拉奉治疗,观察组采用依达拉奉联合神经节苷脂治疗,比较两组神经功能与血清炎性因子[肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)、白细胞介素-6(IL-6)]。结果治疗后,两组NIHSS评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05);治疗后两组血清炎性因子水平显著降低,且观察组CRP、IL-6与TNF-α等指标均低于对照组,差异有统计学意义(P<0.05)。结论急性脑梗死患者采用依达拉奉联合神经节苷脂治疗效果显著优于单一用药,对改善神经功能与血清炎性因子均具有积极作用,值得临床广泛应用。     

疏血通联合依达拉奉治疗老年脑梗死患者的效果

目的:观察疏血通联合依达拉奉治疗老年脑梗死患者的效果。方法:选取2019年2月至2021年3月该院收治的102例老年脑梗死患者进行前瞻性研究,按照随机数字表法分为对照组和观察组各51例。对照组采用依达拉奉治疗,观察组在对照组的基础上联合疏血通治疗,比较两组治疗效果,治疗前后神经功能缺损程度[美国国立卫生研究院卒中量表(NIHSS)]评分、炎性因子[C反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]水平,以及不良反应发生率。结果:观察组治疗总有效率为96.08%,高于对照组的78.43%,差异有统计学意义(P<0.05);治疗后,两组NIHSS评分均低于治疗前,且观察组低于对照组,差异有统计学意义(P<0.05);治疗后,两组CRP、IL-6、TNF-α水平均低于治疗前,且观察组低于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:疏血通联合依达拉奉治疗老年脑梗死患者可提高治疗总有效率,降低NIHSS评分和炎性因子水平,效果优于单纯依达拉奉治疗。     

戊乙奎醚联合依达拉奉对急性脑梗死患者血清IL-1及TNF-α的影响

目的:观察戊乙奎醚联合依达拉奉治疗对急性脑梗死患者血清肿瘤坏死因子(TNF-α)和IL-1影响,探讨戊乙奎醚联合依达拉奉对急性脑梗死患者的治疗价值。方法:将152例急性脑梗死患者随机分为对照组(常规治疗组)、依达拉奉组(常规治疗+依达拉奉)和联合治疗组(常规治疗+依达拉奉+戊乙奎醚),分别检测三组患者治疗前、后的血清TNF-α及IL-1水平。结果:三组病例治疗结束后,血清TNF-α及IL-1评分均有下降,但联合治疗组下降尤为明显。结论:戊乙奎醚联合依达拉奉治疗较单用依达拉奉治疗能进一步降低血清TNF-α及IL-1的含量,从而减轻缺血再灌注损伤和发挥抗炎作用,起到脑保护作用,有利于神经功能的康复。     

依达拉奉与奥拉西坦联合治疗急性脑梗死的临床研究

目的探讨依达拉奉联合奥拉西坦对急性脑梗死患者的疗效及对血清炎性因子与氧化应激指标的影响。方法选取我院2015年1月～2017年1月收治的急性脑梗死患者104例为研究对象,均经临床检查确诊,根据不同治疗方法分为两组,两组患者均给予抗感染、脑细胞保护、颅内压降低、血压控制等常规治疗,对照组(n=52)给予奥拉西坦治疗,观察组(n=52)在对照组基础上给予依达拉奉治疗,对比两组患者临床疗效、治疗前后炎症因子水平以及氧化应激指标水平。结果两组患者总有效率(90.38%vs 73.08%)比较,差异有统计学意义(P0.05)。治疗前两组患者TNF-α、IL-6水平比较差异无统计学意义(P0.05);治疗后观察组患者TNF-α、IL-6水平明显低于对照组,差异有统计学意义(P0.05)。治疗前两组患者MDA、SOD水平比较差异无统计学意义(P0.05);治疗后观察组患者MDA水平明显低于对照组,SOD水平明显高于对照组,差异具有统计学意义(P0.05)。结论依达拉奉联合奥拉西坦两种药物可以进一步降低脑损伤,促进神经功能恢复,因此值得临床推广。     

丁苯酞联合依达拉奉对急性脑梗死患者炎症水平的影响

目的探讨丁苯酞联合依达拉奉对急性脑梗死患者炎症水平的影响。方法选取2016年2月至2018年3月鹤壁市人民医院接收的102例急性脑梗死患者,采用随机数表法分为两组,各51例。对照组接受依达拉奉治疗,观察组在此基础上加用丁苯酞治疗。比较两组血清炎症因子、血清miR-222水平、不良反应。结果治疗后,与对照组比,观察组血清miR-222水平高,IL-8、TNF-α、hs-CRP水平低,差异有统计学意义(均P<0.05)。两组不良反应总发生率差异无统计学意义(P>0.05)。结论丁苯酞联合依达拉奉治疗可有效减轻急性脑梗死患者体内炎症反应。     

丁苯酞联合依达拉奉对急性脑梗死患者TNF-α、IL-1和神经功能恢复的影响

目的：观察丁苯酞与依达拉奉联合应用在治疗急性脑梗死方面的临床疗效,并探讨其对血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和神经功能恢复的影响。方法：选择98例急性脑梗死患者作为研究对象,随机平均分为两组,对照组（49例）给予丁苯酞治疗,研究组（49例）给予丁苯酞联合依达拉奉治疗,两组均治疗14d。疗程结束后,观察两组的临床疗效,检测血清TNF-α和IL-1水平变化,同时对两组患者进行神经功能缺损评分（NIHSS）。结果：治疗后,两组血清TNF-α和IL-1水平均较治疗前明显下降,但研究组下降更明显,差异具有统计学意义（P〈0.05）;两组治疗后NIHSS评分均较治疗前明显降低,但研究组降低更明显,差异具有统计学意义（P〈0.05）。结论：丁苯酞与依达拉奉联合应用能降低急性脑梗死患者的血清TNF-α和IL-1水平,减轻炎症反应和缺血再灌注损伤,临床疗效确切。     

依达拉奉对急性脑梗死患者血清IL-17、IL-6和TNF-α的影响

目的：观察依达拉奉对急性脑梗死患者血清IL-17、IL-6和TNF-α的影响。方法：选择发病24h内的急性脑梗死患者63例,随机分为依达拉奉组和对照组,对照组31例按急性脑梗死常规方法治疗2周,依达拉奉组32例在常规治疗基础上加用依达拉奉治疗2周,治疗前、治疗后7d、14d检测患者血清IL-17、IL-6和TNF-α。结果：两组患者治疗前及治疗7d IL-17、IL-6、TNF-α水平无统计学差异,但治疗14d依达拉奉组各项指标均低于对照组,差异有统计学意义。两组患者治疗7d和14d IL-17、IL-6、TNF-α水平均低于治疗前,差异有统计学意义。结论：依达拉奉能显著降低急性脑梗死患者血清炎症因子IL-17、IL-6和TNF—α的水平,这可能是其治疗脑梗死的机制之一。     

丁苯酞、依达拉奉对老年脑小血管病患者血清炎性因子及血管内皮功能的影响

目的 探究丁苯酞、依达拉奉对老年脑小血管病(CSVD)患者血清炎性因子及血管内皮功能的影响。方法 选取老年CSVD患者82例为研究对象，随机分为丁苯酞注射液组(n=41)和依达拉奉注射液组(n=41)。依达拉奉注射液组采用依达拉奉注射液治疗，丁苯酞注射液组采用丁苯酞注射液治疗。治疗14 d后比较两组的治疗总有效率、治疗前后肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、白介素8(IL-8)等血清炎性因子，一氧化氮(NO)、血管内皮生长因子(VEGF)等血管内皮功能因子及美国国立研究院脑卒中量表(NIHSS)评分，并进行为期1年的随访，记录患者预后情况。结果 丁苯酞注射液组的治疗总有效率(90.24%)高于依达拉奉注射液组总有效率(75.61%)(P<0.05)。治疗后两组的TNF-α、IL-6、IL-8水平均降低，丁苯酞注射液组TNF-α、IL-6、IL-81水平均低于依达拉奉注射液组(P<0.05)。治疗后两组的NO水平升高、VEGF水平降低，且丁苯酞注射液组NO水平高于依达拉奉注射液组(P<0.05),VEGF水平低于依达拉奉注射液组(P<0.05)。...     

丁苯酞软胶囊联合依达拉奉注射液治疗脑梗死的效果及对神经功能的影响

目的 探究丁苯酞胶囊联合依达拉奉注射液治疗脑梗死患者的治疗效果及对患者神经功能的影响.方法 选取本院2019年4月至2020年4月收治的脑梗死患者84例,依据摸球法分为两组,各42例.对照组采用依达拉奉注射液治疗,观察组采用丁苯酞胶囊联合依达拉奉注射液治疗.比较两组治疗效果、不良反应与治疗前后的神经功能、炎性因子、血清神经营养因子、日常生活能力等多个指标情况.结果 观察组治疗总有效率为92.86％,高于对照组的71.43％,差异有统计学意义(P<0.05);观察组不良反应发生率为2.38％,低于对照组的7.14％,差异无统计学意义;治疗后,观察组神经功能与日常生活能力评分均优于对照组(P<0.05);两组治疗后炎性因子比较差异有统计学意义(P<0.05);组间血清神经营养因子比较差异有统计学意义(P<0.05).结论 丁苯酞胶囊联合依达拉奉注射液治疗脑梗死患者,病情显著改善,且不良反应较少,可有效改善患者炎性因子,促进患者神经功能与日常生活能力恢复,值得临床推广.     

瑞舒伐他汀联合依达拉奉治疗急性脑梗死的效果及炎性因子水平分析

目的：探讨、分析瑞舒伐他汀联合依达拉奉治疗急性脑梗死的效果及炎性因子水平。方法：回顾性选取80例急性脑梗死患者作为研究对象,按照不同的治疗方法将患者分为单一组与联合组各40例。单一组采用依达拉奉治疗,联合组采用依达拉奉联合瑞舒伐他汀治疗,对比两组患者的治疗前后炎性因子[超敏C-反应蛋白(hs-CRP)、基质金属蛋白酶-9(MMP-9)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]水平与疗效。结果：治疗后单一组与联合组的hs-CRP分别为(38.69±3.34)mg/L、(25.88±2.70)mg/L,MMP-9分别为(45.23±5.03)ng/L、(37.68±3.95)ng/L,IL-6分别为(66.24±8.01)ng/L、(51.33±7.24)ng/L,TNF-α分别为(44.09±5.28)ng/L、(30.81±4.43)ng/L,治疗后联合组的hs-CRP、MMP-9、IL-6、TNF-α均低于单一组,差异有统计学意义(P<0.05);单一组与联合组的治疗总有效率分别为62.50%、85.00%,联合组的治疗总有效率更高,差异有统计学意义(P&l...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.