动态三维子宫输卵管超声造影在输卵管阻塞中的应用

目的探讨动态三维子宫输卵管超声造影在输卵管阻塞中的应用。方法选取2015年6月~2016年10月来本院就诊的不孕症患者120例,均行动态三维子宫输卵管超声造影(3D-Hy Co Sy)和X线下输卵管碘海醇注射液造影检查,并与腹腔镜通染液实验(laparoscopy and dye test,CLP)就灵敏度、特异性、准确性进行比较。结果本研究40例患者,共80条输卵管,经3D-Hy Co Sy检测显示输卵管通畅48条,通而不畅15条,阻塞17条;经X线下输卵管碘海醇注射液造影检测显示输卵管通畅47条,通而不畅16条,阻塞17条。两种方法总体符合率为87.50%,有高度一致性(P=0.00,Kappa值=0.779)。以CLP检测结果为对照,3D-Hy Co Sy检测的灵敏度、特异性、准确性分别为90.90%、93.62%、92.50%;而X线下输卵管碘海醇注射液造影检测的灵敏度、特异性、准确性分别为87.88%、89.36%、88.75%。结论在输卵管阻塞患者中实施3D-Hy Co Sy较X线下输卵管碘海醇注射液造影更能全面、客观、安全的显示患者输卵管通畅情况,就其综合优点来分析,有望成为日后输卵管阻塞患者诊治的主要检测方法。     

3D-Hy CoS检查对女性输卵管阻塞不孕患者诊断准确率的影响

目的:探讨经阴道三维超声输卵管造影(3D-HyCoSy)检查对女性输卵管阻塞不孕患者诊断准确率的影响。方法:选取我院就诊的不孕患者122例(244条输卵管),所有患者均行3D-HyCoSy、X线下输卵管碘海醇注射液造影、腹腔镜通染液实验(CLP)检查,并以CLP检查结果作为参照,统计对比3D-HyCoSy、X线下输卵管碘海醇注射液造影诊断结果。结果:以CLP诊断结果作为参照,3D-HyCoSy诊断出输卵管阻塞93条,X线下输卵管碘海醇注射液造影诊断出输卵管阻塞75条,3D-HyCoSy诊断特异度(92.91%)、敏感度(90.29%)、准确率(91.80%)均较X线下输卵管碘海醇注射液造影(79.43%、72.82%、76.64%)高(P<0.05)。结论:3D-Hy CoS检查可提高女性输卵管阻塞不孕患者诊断特异度、敏感度、准确率,为临床诊治提供重要参考依据。     

三维子宫输卵管超声造影对不孕症的诊断价值

目的:评价经阴道三维子宫输卵管超声造影对输卵管阻塞病变诊断的临床价值.方法:对103例不孕患者以第2代造影剂SonoVue进行三维超声造影重建术.结果:阴道三维子宫输卵管超声造影显示103例患者200条输卵管中通畅127条,通而不畅42条,阻塞31条;103例患者单侧输卵管不通23例,双侧输卵管不通4例.结论:三维子宫输卵管超声造影是一种可靠、直观、准确评价输卵管通畅性的有效方法.     

经阴道四维子宫输卵管超声造影对输卵管性不孕的临床诊断价值

目的：探讨经阴道四维子宫输卵管超声造影对输卵管性不孕的临床诊断价值。方法：选取2021年2月-2022年2月阳东区人民医院收治的60例输卵管性不孕患者为研究对象,分别对患者实施经阴道四维子宫输卵管超声造影检查与腹腔镜检查,比较两种检查方式对输卵管通畅性的检查结果、不良反应发生率及经阴道四维子宫输卵管超声造影检查结果情况。结果：两种检查方式输卵管的通畅、通而不畅、阻塞检出率比较,差异无统计学意义(P>0.05)。经阴道四维子宫输卵管超声造影检查的不良反应发生率高于腹腔镜,差异有统计学意义(P<0.05)。经阴道四维子宫输卵管超声造影检查结果为60例患者共检测120条输卵管,其中输卵管通畅50条,输卵管通而不畅40条,输卵管阻塞30条。结论：经阴道四维子宫输卵管超声造影对输卵管因素不孕症的临床诊断具有较高价值,可作为首选检测。     

二维子宫输卵管超声造影(2D-HyCoSy)与子宫输卵管造影(HSG)评价输卵管通畅性的价值研究

目的分析及对比二维子宫输卵管超声造影(2D-Hy Co Sy)和子宫输卵管造影(HSG)在评估输卵管通畅性中的应用价值。方法收集本科室2015年8月至2016年8月间接收的患不孕症的50例妇女进行研究,均施行2D-Hy Co Sy检查所有患者的输卵管状况,且与患者的HSG检查结果进行比较分析。结果 2种检查方法的结果对比差异无统计学意义(P0.05)。结论 2D-Hy Co Sy在评估输卵管通畅性中的应用价值与HSG相当,值得推广。     

三维子宫输卵管超声造影与X线子宫输卵管造影对输卵管通畅性诊断的比较研究

目的探讨三维子宫输卵管超声造影与X线子宫输卵管造影对输卵管通畅性诊断的临床价值。方法收集整理齐齐哈尔医学院附属第二医院不孕患者的临床资料,比较三维子宫输卵管超声造影与X线子宫输卵管造影对输卵管通畅性诊断的结果。结果三维子宫输卵管超声造影检出输卵管不通畅率为96.08%,X线子宫输卵管造影检出输卵管不通畅率为62.75%,三维子宫输卵管超声造影检出输卵管不通率明显高于X线子宫输卵管造影(χ²_0.05,1=10.67,P=0.001),差异有统计学意义。结论三维子宫输卵管超声造影对输卵管通畅性有较高的诊断精准性,临床应用价值高,可以作为临床首选检查手段。     

实时三维子宫输卵管超声造影评估子宫内膜异位症患者输卵管通畅程度的效果分析

目的 探讨实时三维子宫输卵管超声造影评估子宫内膜异位症患者输卵管通畅程度的临床效果。方法以2020年1月—2021年3月惠东县妇幼保健计划生育服务中心收治的72例子宫内膜异位症患者为研究对象,全部患者均以实时三维子宫输卵管超声造影检查输卵管通畅度,评价图像质量,统计患者造影后不良反应,并以腹腔镜下输卵管通液术诊断结果为金标准,评价实时三维子宫输卵管超声造影对输卵管通畅性的诊断效能。结果 72例患者实时三维子宫输卵管超声造影图像质量优良率93.06%,患者造影后不良反应率12.50%,均为一过性症状。输卵管通液术诊断输卵管通畅107条（74.31%）、通而不畅13条（9.03%）、阻塞24条（16.67%）,三维子宫输卵管超声造影诊断输卵管通畅性的灵敏度为94.59%(35/37),特异度为93.46%(100/107),诊断总符合率93.75%(135/144)。结论 实时三维子宫输卵管超声造影对子宫内膜异位症患者输卵管通畅程度具有较高的诊断效能,应用安全有效。     

多维子宫输卵管超声造影对输卵管性不孕症患者的诊断效果评价

目的：探讨与评价多维子宫输卵管超声造影对输卵管性不孕症患者的诊断效果。方法：选择2020年1月—2021年12月在本院进行诊断的输卵管性不孕症患者72例作为观察对象。所有患者均给予多维子宫输卵管超声造影及盆腔水造影检查,同时给予腹腔镜下输卵管通液术检查并判断诊断价值。结果：在72例(共144条输卵管)患者中,腹腔镜下输卵管通液术判断为输卵管通畅56条,输卵管阻塞88条。多维子宫输卵管超声造影判断为输卵管通畅54条,输卵管阻塞90条。盆腔水造影判断为输卵管通畅47条,输卵管阻塞97条。多维子宫输卵管超声造影诊断输卵管阻塞的敏感性、准确性为96.4%、98.6%,均明显高于盆腔水造影的80.4%、91.0%(P<0.05);而多维子宫输卵管超声造影诊断输卵管阻塞的特异性为100.0%,与盆腔水造影的97.7%对比无明显差异(P>0.05)。结论：多维子宫输卵管超声造影在输卵管通畅性评估评价中具有较好的效果,能提高输卵管性不孕症患者的诊断敏感性与准确性,可为不孕症的诊断筛查提供依据。     

经阴道四维子宫输卵管超声造影在女性不孕症诊断中的临床应用

目的 探讨经阴道子宫输卵管四维超声造影在女性不孕症中的临床应用价值.方法 回顾性分析接受子宫输卵管四维超声造影的不孕症患者45例患者的临床资料.结果 45例90条输卵管四维子宫输卵管超声造影显示输卵管通畅59条,输卵管不通畅21条,输卵管通而不畅10条;造影显示不全纵膈子宫3例、单角子宫1例.四维子宫输卵管超声造影诊断输卵管阻塞15例共21条输卵管,其中双侧阻塞6例,单侧阻塞9例,均进行了腹腔镜检查与治疗,1例因输卵管壶腹部粘连扭曲,伞端粘连于腹壁超声造影诊断阻塞,腹腔镜为通而不畅,其余20条超声造影结果均与腹腔镜结果一致,两者符合率为95.2％.结论 子宫输卵管四维超声造影可实时动态观察子宫腔情况及输卵管各段通畅性,是不孕症患者诊治前观察和评估输卵管通畅性的重要影像方法.     

三维超声输卵管造影与二维超声输卵管造影的对比研究

 目的 对比子宫输卵管三维超声编码造影技术与二维超声造影诊断不孕症患者输卵管通畅性的临床价值。方法 对拟在我院进行腹腔镜通染术的70例不孕患者于术前进行超声造影检查。随机分组后,30例进行二维超声造影,40例进行三维超声编码造影。注入SonoVue后,评估输卵管通畅性。造影后3~7 d进行腹腔镜通染术。结果 二维超声造影灵敏度92.3%、特异度91.2%、阳性预测值88.9%、阴性预测值93.9% 。三维超声编码造影技术灵敏度94.7%、特异度92.9%、阳性预测值92.3%、阴性预测值95.1%;三维超声造影的ROC曲线下面积0.951,二维超声造影的ROC曲线下面积0.849,二者差异具有统计学意义（P=0.021）。结论3D-HyCoSy的最新技术CCI造影对子宫宫腔病变和输卵管通畅性的诊断和鉴别有着明显优势。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.