5种过敏性皮肤病儿童患者皮肤点刺试验结果分析

目的 了解儿童过敏性皮肤病的变应原情况.方法 采用德国默克公司生产的阿罗格变应原点刺液对5种常见过敏性皮肤病的儿童患者进行点刺试验.结果 例过敏性皮肤病患者的变应原检测阳性率为75.22%;变应原中粉尘螨(63.15%)和屋尘螨(58.62%)阳性率最高,反应强度亦最大;特应性皮炎(87.50%)对变应原反应阳性率最高...     

835例变态反应性皮肤病变应原点刺试验结果分析

目的探讨皮肤变应原点刺试验在皮肤变态反应性疾病诊断中的意义。方法选用德国默克公司生产的阿罗格变应原点刺液17种进行试验，阳性对照用组胺，阴性对照用生理盐水，分别对835例荨麻疹、丘疹性荨麻疹等疾病进行皮肤点刺试验，大于3种过敏原阳性者和／或强阳性者为高敏感者。结果619例阳性（阳性率74．13％），高敏感者336例（40．24％），阳性率前五位的变应原分别为粉尘螨（58．44％）、屋尘螨（54．25％）、霉菌Ⅰ（32．8I％）、霉菌Ⅱ（27．31％）、树Ⅰ（19．04％）。随着年龄的增大，变应原．点刺试验阳性率呈阶梯状下降趋势（P〈0．01）。荨麻疹（83．29％）与丘疹性荨麻疹（80．14％）对变应原反应阳性率较高。结论粉尘螨和屋尘螨是本地区变态反应性皮肤病的最主要的变应原，许多患者可以对多种变应原产生过敏，可以出现强阳性，点刺阳性率随年龄的增大呈下降趋势。皮肤变应原点刺试验可以为变态反应性皮肤病患者避免接触相应过敏原和进行特异性脱敏治疗提供客观依据。     

1 195例过敏性皮肤病变应原皮肤点刺试验结果分析

目的:了解常见的4种过敏性皮肤病的变应原分布情况。方法:采用皮肤点刺法对1195例过敏性皮肤病患者进行变应原测试。结果:慢性荨麻疹的阳性率最高。最常见的食入性变应原是海虾、海蟹、带鱼、芝麻、花生,吸入性过敏原是蟑螂、粉尘螨、室内尘、油菜花粉、柳树。结论:皮肤点刺试验可以帮助患者找出致病原,从而避免接触致病变应原。并为脱敏治疗提供依据。     

皮肤点刺试验检测慢性荨麻疹变应原的临床意义

目的探讨皮肤点刺试验检测慢性荨麻疹变应原的临床意义。方法用20种不同的变应原进行皮肤点刺试验,生理盐水为阴性对照,组胺为阳性对照。结果292例慢性荨麻疹患者中,有210例为阳性反应,阳性率为71.92%,单个变应原中,以吸入性粉尘螨的阳性率为最高,达63.69%。结论皮肤点刺试验特异性高,为慢性荨麻疹患者寻找可能的变应原提供依据,并力求避免接触,以提高患者的生活质量。     

慢性荨麻疹400例变应原检测结果分析

目的探讨各种变应原与慢性荨麻疹发病的关系,为患者的预防提供指导依据。方法采用国际标准的皮肤点刺试验对400例慢性荨麻疹患者进行23种变应原的检测。结果400例慢性荨麻疹患者进行23种变应原的检测阳性率达65.75%(263例):变应原中粉尘螨45.25%(181例)阳性率最高,其次为螨Ⅱ36.50%(146例)。结论对于慢性荨麻疹患者在诊疗过程中应积极寻找病因,避免在日常生活中接触相应的致病变应原,降低发病率。     

变态反应性皮肤病常见变应原检测结果分析

用尘螨、屋尘、猫毛等20种常见吸入和食物变应原对1500例变态反应性皮肤病患者进行皮肤点刺试验。1500例患者中过敏原阳性746例（81．9％），吸入组中粉尘螨皮试阳性率最高（58．3％），其次是屋尘（52．7％）；食物组中海虾阳性率最高（39．1％），其次是海蟹（37．8％）。对于粉尘螨、屋尘，14～17岁组最高，2～6岁组最低。广州地区变态反应性皮肤病主要变应原为粉尘螨、屋尘。     

荨麻疹1000例变应原皮肤点刺试验结果分析

目的分析1000例荨麻疹患者应用点刺液变应原皮肤试验结果,为本地区荨麻疹的防治及点刺试验变应原的选择提供依据。方法用30种变应原,加一个生理盐水阴性对照和一个组胺阳性对照,试验部位是前臂掌侧皮肤,相距4 cm,滴1滴变应原后垂直点刺,20-30 min后判读试验结果。结果765例(76.5%)呈阳性。皮肤点刺试验中屋尘螨阳性率最高,其次是粉尘螨等。结论屋尘螨和粉尘螨是北京地区重要变应原。变应原皮肤点刺试验用于寻找荨麻疹患者的致病原因安全有效。     

特应性皮炎患者过敏原两种检测方法的相关性

目的探讨皮肤点刺试验和血清变应原特异性IgE检测的相关性。方法同时采用皮肤点刺试验(SPT)和德国MEDIwlss敏筛过敏原检测系统检测60例特应性皮炎(AD)患者的过敏原,并对检测结果进行比较。结果皮肤点刺试验和血清变应原特异性IgE的阳性率分别为3.89%(140/3600)和7.42%(89/1200),后者明显高于前者,差异有统计学意义(P=0.000),且这两种检测方法不相关(P=0.207)。结论皮肤点刺试验和血清变应原特异性IgE检测特应性皮炎过敏原不相关,必要时可同时进行这两种方法检测,并结合病史判断病情。     

广州地区1232例慢性过敏性皮肤病患者皮肤点刺试验的结果分析

目的：探讨慢性过敏性皮肤病常见变应原的致病状况,为其流行病学研究及临床诊断、治疗和预防提供依据.方法：应用德国默克公司生产的 Allergopharma标准变应原液在前臂掌侧皮肤进行皮肤点刺试验, 同时应用0.1%组胺和生理盐水作为阳性和阴性对照,判断结果得出的数据用SPSS13.0软件包进行2检验和t检验.结果：1 232例慢性荨麻疹、特应性皮炎、慢性湿疹等患者的皮肤点刺试验中,结果变应原阳性率为81.3%;尘螨（屋尘螨＋粉尘螨） 阳性率（57.0%）明显高于其它变应原,其次阳性率较高的有虾（18.1%）,其余阳性率〉10%的有松属、禾本科花粉、霉菌、杂草.儿童组阳性率明显高于成人组（P〈0.01）,吸入组变应原阳性率及阳性程度明显高于食物组变应原（P〈0.01）.结论：吸入组变应原是慢性过敏性皮肤病常见变应原,尘螨为首要变应原,其儿童组阳性率明显高于成人组,反应的强度明显强于成人组（P〈0.01）.     

天津地区296例过敏性疾病皮肤点刺试验结果分析

目的分析天津地区296例过敏性疾病患者的常见变应原。方法采用皮肤点刺试验检测天津地区296例过敏性疾病患者的常见过敏原并对其结果进行分析。结果 296例患者中阳性患者125例,总阳性率为42.23%;其中粉尘螨、屋尘螨阳性率最高,分别为17.91%和15.20%,其次是蟑螂、艾蒿、干草尘埃,分别占9.12%、8.78%和6.08%。荨麻疹、湿疹和特应性皮炎患者的阳性率最高,分别为44.83%、42.64%和40.0%。结论粉尘螨和屋尘螨是天津地区过敏性疾病患者的常见致敏原,其次是蟑螂、艾蒿、干草尘埃,点刺试验是检测过敏原的重要手段。     

Positive patch tests with a dermatophagoides mix relate to an increased responsiveness to standard patch test allergens

The diagnostic meaningfulness of patch tests with house dust mite allergens is still questionable. Our own impression has been that positive results with a dermatophagoides mix may occur preferentially in patients with a generally enhanced responsiveness to contact allergens. To check this, all of our patients allocated to patch testing with the standard series were additionally patch tested with a dermatophagoides mix by the same technique that was used for standard contact allergens. Out of 571 patients tested, 188 showed delayed responses to this mix that were indistinguishable from typical allergic patch test reactions but of no apparent clinical relevance. No relationship was found between positive dermatophagoides patch tests and an atopic disposition of the patients or characteristics of their eczema. However, 64.4% of the patients with a positive dermatophagoides patch test showed a response to at least 1 contact allergen of the standard series, compared to only 56.4% of the patients without a positive dermatophagoides reaction (p < 0.05). The reactivity to the mite mix was not related to the responsiveness towards any particular contact allergens. We suppose that some unidentified factors may contribute to positive reactions to the dermatophagoides mix that may also favour an enhanced general responsiveness to contact allergens.     

大连地区121例过敏性疾病患儿皮肤点刺试验结果分析

目的:确定大连地区过敏性疾病患儿过敏原的种类。方法:对121例过敏性疾病患儿进行皮肤点刺试验(SPT)。结果:检测阳性率为76%(92例);居于前5位的过敏原为粉尘螨(47.9%)、屋尘螨(45.5%)、须发癣菌(29.8%)、霉菌II(28.1%)和霉菌I(24.8%)。结论:吸入性过敏原为大连地区过敏性疾病患儿的主要致敏原。     

The detection of clinically relevant contact allergens with a standard screening tray of 28 allergens

Background. A standard method for diagnosing allergic contact dermatitis in the United States is the Thin‐layer Rapid Use Epicutaneous (TRUE) test (TRUE Test?), which consists of three panels containing 20 individual allergens and eight allergen mixes. Previous studies had raised concern regarding the adequacy of the initial two‐panel TRUE Test? system (16 individual allergens and seven allergen mixes) in fully assessing patients with possible allergic contact dermatitis. Objectives. We sought to investigate the effectiveness of the current three‐panel TRUE Test? as the sole diagnostic tool for detecting allergic contact dermatitis. Patients/materials/methods. This study was a retrospective analysis of 2088 patients who underwent patch testing between 1995 and 2010. Study groups were analysed to determine whether positive reactions were to allergens and/or mixes present in the TRUE Test? panels. Results. Of the 2088 patch‐tested patients, 1385 had at least one positive reaction. Among these 1385 patients, 27.6% were fully evaluated by use of only the TRUE Test? series, 49.9% were partially evaluated, and 22.5% did not have any of their allergens detected. On assessment for clinical relevance, similar percentages were observed. Conclusion. In our study, the current TRUE Test? series of 28 allergens would have completely identified allergens in only 27.6% of patients. Broadening the standard panel to include common allergens causing >50% of allergic contact dermatitis cases in a given geographical location and aim testing allergens on the basis of the patient's history will increase the test's sensitivity.     

反复发作性过敏性紫癜患儿血清过敏原特异性IgE检测

目的进一步了解血清过敏原特异性IgE在儿童反复发作性过敏性紫癜中的作用,探讨各种变应原与疾病发病的关系。方法采用酶联免疫吸附试验检测130例反复发作性过敏性紫癜患儿的血清过敏原特异性IgE。结果130例患儿中血清总IgE水平>50IU/ml者103例,阳性率80.47%。其中73例(75.3%)对两种以上过敏原呈阳性反应,30例(24.7%)对一种过敏原呈阳性反应。常见的吸入性过敏原为霉菌(29/130)、蒿类花粉(16/130)和尘螨(15/130)。食物组最常见的为牛奶(35/130)、蛋白类(26/130)、鱼类(15/130)和虾蟹(12/130)。结论反复发作性过敏性紫癜的发生与吸入性和食入性过敏原都有一定的相关性,霉菌和蛋白类食物是诱导反复发作性过敏性紫癜的主要致病原。特异性IgE检测可帮助筛选过敏原,对疾病的预防具有积极意义。     

深圳地区荨麻疹患儿血清过敏原特异性IgE的检测分析

目的探讨深圳地区儿童荨麻疹的发生与过敏原之间的关系：方法采用过敏原体外检测试剂盒对417例荨麻疹患儿进行血清过敏原特异性IgE的检测.结果351例（84．17％）患儿过敏原筛查阳性,其中172例（41．25％）患儿对2项或2项以上过敏原过敏;吸入组主要对粉尘螨、屋尘、点青霉等真菌及夏季花草过敏,且对粉尘螨、屋尘、点青霉等真菌过敏的阳性率随年龄增长而增高;食物组主要对鱼类、虾、蟹、贝及菇粪过敏,且对鱼类、虾、蟹、贝过敏的阳性率随着年龄增长而下降.结论深圳地区患儿荨麻疹的发生与过敏原有密切关系．粉尘螨、霉菌、夏车花草、海鲜及菇类是深圳地区荨麻疹患儿常见的病因．     

大连地区变应性疾病变应原分析

目的：探讨大连地区引起过敏性疾病的主要过敏原及过敏原在不同性别、年龄患者中可能存在的差异。方法：回顾性研究我科门诊变应性疾病1059例患者点刺试验结果。结果：过敏性疾病过敏原阳性率为92.4％,居前5位的过敏原分别为屋尘螨45.8％、粉尘螨42.3％、须发癣菌34.1％、霉菌Ⅱ27％ 、霉菌Ⅰ21.7％。3种以上过敏原阳性患者占74.9％。须发癣菌、干草尘埃、虾类、鸡蛋、羊肉过敏阳性率与性别有关。须发癣菌和霉菌Ⅰ的过敏阳性率与年龄有关。结论：屋尘螨、粉尘螨、须发癣菌、霉菌是大连地区最常见的致敏原,男性须发癣菌、干草尘埃、虾类、鸡蛋、羊肉过敏阳性率明显高于女性。老年人对须发癣菌和霉菌过敏阳性率明显增高。     

816例皮肤点刺试验过敏原结果分析

目的 探讨特应性皮炎、慢性荨麻疹、慢性湿疹常见过敏原的可能致病情况.方法 用标准变应原液在前臂屈侧皮肤进行皮肤点刺试验,分别用0.1%组胺和0.9%氯化钠液作阳性和阴性对照,统计分析采用x2检验.结果 尘螨(屋尘螨+粉尘螨)的阳性率最高,达50.0%～78.6%,其次为动物毛,达7.1%～21.4%,霉菌的阳性率也较高,达7.1%～14.3%,并且这3种变应原在3种疾病间的阳性率差异均有统计学意义(P＜0.01),以特异性皮炎的阳性率最高.食物组过敏原中虾的阳性率(6.6%～14.3%)最高,但在3种疾病间的阳性率差异无统计学意义(P＞0.05);金枪鱼在慢性荨麻疹中的阳性率(9.5%)较高,鸡蛋、牛奶、芹菜在特异性皮炎中的阳性率(7.1%～14.3%)较高,这4种变应原在3种疾病间的阳性率差异均有统计学意义(P＜0.05).结论 吸入组过敏原是慢性过敏性皮肤病的常见过敏原,尘螨为首要过敏原,以特异性皮炎的阳性率为最高.     

Comparative multi-center study with TRUE Test and Finn Chamber Patch Test methods in eight Swedish hospitals

292 individuals were patch tested with twelve allergens, using a new patch test technique, TRUE Test (TT). As controls, the same allergens in standard concentrations in petrolatum were applied using the Finn Chamber technique (FC). The allergen doses used in TT were chosen according to results from a previous serial dilution patch test study. There were reactors to all twelve allergens. The concordance of positive reactions between TT and FC was 78%. 10% were indicated only with TT and 12% FC only. Irritant reactions occurred in the same order of magnitude for the two tests. Weak positive, uncertain, and irritant reactions observed with the different test methods used, indicate minor errors of allergen dosage in both. The investigation indicates that the TRUE Test method is simple to handle, is well standardized and gives good accuracy with the 12 allergens investigated.