HLA-DQA1及DQB1等位基因与寻常型银屑病遗传易感性研究

目的 探讨HLA-DQA1和DQB1等位基因与汉族人寻常型银屑病遗传易感性。方法 利用聚合酶链反应-序列特异引物(PCR-SSP)法,对189例银屑病患者和273例健康人的HLA-DQA1和DQB1等位基因进行检测。结果 ①HLA-DQA1*0104和DQA1*0201与汉族人银屑病呈正相关性(Pc<0.05);DQA1*0501与汉族人银屑病呈负相关(Pc<0.001).②HLA-DQA1*0104、DQA1*0201和DQA1*0501等位基因与Ⅰ型银屑病发病有关。③HLA-DQA1*0104和DQA1*0201等位基因在有家族史和无家族史患者中的频率显着性增高。HLA-DQA1*0501仅在无家族史银屑病患者中显着性下降。结论 ①HLA-DQA1*0104和DQA1*0201可能是银屑病的易感基因或与易感基因相连锁;DQA1*0501等位基因可能具有阻止汉族人发生银屑病的作用。②有家族史和无家族史银屑病患者在其遗传背景上可能存在差异。     

兰州汉族人寻常性银屑病与HLA-DQA1*0104和*0201的相关性

目的探讨HLA-DQA1*0104和*0201等位基因与兰州汉族寻常性银屑病的相关性。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)方法分别检测HLA-DQA1*0104和*0201等位基因在60例寻常性银屑病患者及56例健康对照中的分布频率,并进行比较。结果寻常性银屑病患者中HLA-DQA1*0104和*0201等位基因频率(43.33%和36.67%)分别与健康对照组(17.86%,14.29%)比较,差异均有统计学意义(P均<0.05)。结论 HLA-DQA1*0104和*0201可能是兰州汉族寻常性银屑病的易感基因。     

北方汉族寻常型银屑病与HLA-DRB1及DQB1等位基因相关性研究

目的:探讨HLA-DRB1及DQB1等位基因与北方汉族寻常型银屑病相关性。方法:利用序列特异性引物-聚合酶链反应(PCR-SSP)分型技术,对63例寻常型银屑病患者和102例健康人的HIA-DRB!及DQB1等位基因进行检测。结果:(1)HLA-DRB1*070x、DRB1*1001及DOB`*020x等位基因与北方汉族寻常型银屑病呈正相关(P分别为0.001,0.005,0.009);HLA-DRB1*120x等位基因与北方汉族寻常型银屑病呈负相关(P=0.007)。(2)HLA-DRB1*070x及DQB1*020x等位基因仅与家族史阳性的早发型(Ⅰ型)银屑病发病相关(P<0.001)。(3)HLA-DRBq*1001等位基因频率在Ⅰ型及无家族史的晚发型(Ⅱ型)银屑病均显著性增高(P<0.05)。结论:(1)HLA-DRB1*070x、DRB1*1001及DQB1*020x等位基因可能是北方汉族寻常型银屑病的易感基因或与易感基因相连锁;HLA-DRB1*120x等位基因可能是阻止北方汉族人发生银屑病的保护基因。(2)Ⅰ型及Ⅱ型银屑病的遗传背景存在差异。     

包头地区寻常性银屑病与HLA-Cw* 0602等位基因的相关性研究

目的探讨包头地区汉族寻常性银屑病患者与HLACw0602等位基因的相关性。方法采用聚合酶链反应序列特异引物(PCRSSP)法,检测52例寻常性银屑病患者及60名健康对照者的等位基因频率,并相互比较。结果①HLACw0602与包头地区汉族寻常性银屑病患者具有明显的相关性(OR=3.47,P<0.01);②HLACw0602在Ⅰ型、Ⅱ型寻常性银屑病患者中分布无差异(χ2=0.006,P>0.05)。结论HLACw0602可能是寻常性银屑病易感基因或与易感基因相连锁。     

银屑病中医证型与HLA等位基因的相关性

目的:探讨银屑病中医证型与人类白细胞抗原(HLA)-CW、-DRB1等位基因的相关性。方法:用序列特异性引物-聚合酶链反应(PCR-SSP)方法,对152例江苏籍汉族寻常性银屑病患者进行HLA-CW、-DRB1等位基因的分型,并分析上述基因在各组中的分布。结果:银屑病患者的两个等位基因阳性率均高于对照组,差异有统计学意义,HLA-CW*0602等位基因:OR=5.35,P0.05。HLA-DRB1*07等位基因:OR=3.49,P0.05。血热型基因阳性率与血瘀型基因阳性率相比,P=0.586,差异无统计学意义(P0.05)。结论:HLA-CW*0602与HLA-DRB1*07基因可能是江苏籍寻常性银屑病患者易感基因。银屑病患者的这两种基因与中医证型无相关性。     

壮族人寻常性银屑病与HLA-DQA1和DQB1基因的相关性研究

[摘要]目的：探讨广西壮族人寻常型银屑病的发病与HLA-DQA1和DQB1基因的关联。方法：应用聚合酶链式反应-序列特异引物（PCR-SSP）法对58例壮族寻常型银屑病患者和102例健康壮族人的HLA-DQA1和DQB1座位进行基因分型，比较两组相应等位基因的频率。结果：HLA-DQB1*0303与壮族银屑病患者呈显著的正相关（OR=4.540，p=0.004），而HLA-DQA1*0501和HLA-DQB1*0301与壮族银屑病患者呈显著的负相关（OR=0.189，p=0.000；OR=0.367，p=0.018）。结论：以上3个HLA-DQ等位基因与广西壮族人寻常型银屑病的关系密切，其中HLA-DQB1*0303可能为该人群银屑病的易感因子，而HLA-DQA1*0501和HLA-DQB1*0301则可能对银屑病有抵抗作用。     

内蒙古蒙古族寻常性银屑病与HLA-Cw*0602,-DQB1等位基因的相关性

目的探讨内蒙古蒙古族寻常性银屑病与HLA-Cw*0602,-DQB1等位基因的关联性。方法利用序列特异性引物-聚合酶链反应(PCR-SSP)分型技术,对蒙古族寻常性银屑病患者65例及对照组正常蒙古族60例样本进行分型检测并比较分析。结果①寻常性银屑病患者组HLA-Cw*0602,-DQB1*0201等位基因频率较对照组明显升高,差异有统计学意义(P0.05);②HLA-Cw*0602,-DQB1*0201在Ⅰ型及家族史阳性银屑病患者中显著升高(P0.05);③HLA-DQB1*0301在患者组中有显著下降(P0.05)。结论①HLA-Cw*0602及-DQB1*0201可能是内蒙古地区蒙古族寻常性银屑病的易感基因;有家族史和无家族史银屑病患者可能存在遗传背景上的差异。     

白癜风125例临床分析及与HLA-DRB1相关性研究

分析中国北方汉族白癜风的临床特征及与HLA-DRB1等位基因的相关性,探讨白癜风的病因及发病机制.登记125例白癜风门诊患者的临床资料,利用聚合酶链反应-序列特异引物(PCR-SSP)技术对125例白癜风患者和263例健康人进行等位基因检测,对各项指标进行统计分析.结果,白癜风初发年龄男性与女性无显著差异;家族史阳性患者发病年龄早于家族史阴性患者;伴有自身免疫性疾病平均初发年龄大于不伴自身免疫疾病的白癜风患者;白癜风与HLA-DRB1*070X及HLA-DRB1*1201等位基因有相关性.①有家族史和无家族史白癜风患者在遗传背景上存在差异,HLA-DRB1*070X可能为家族史阳性患者的易感基因.②提示了节段型与非节段型白癜风的发病机制有所不同,泛发性及散发性与自身免疫相关明显.③HLA-DRB1*070X及HLA-DRB1*1201可能是中国北方汉族寻常型白癜风的易感基因或与易感基因相连锁.     

兰州地区汉族寻常性、脓疱性银屑病与HLA-DRB1*0701的相关性

目的探讨兰州地区汉族寻常性、脓疱性银屑病与HLA-DRB1*0701等位基因的相关性。方法采用聚合酶链反应-序列特异引物(PCR-SSP)法检测42例寻常性银屑病、28例脓疱性银屑病和50例健康对照者HLA-DRB1*0701等位基因频率,并相互比较。结果寻常性银屑病患者组及脓疱性银屑病患者组HLA-DRB1*0701等位基因频率分别(54.8%,46.4%)与正常对照组(22.0%)比较差异均有显著性(P<0.05)。结论HLA-DRB1*0701等位基因可能是兰州地区汉族寻常性、脓疱性银屑病的遗传标志。     

兰州地区汉族寻常型、关节病型银屑病与HLA-DQB1*0201相关性研究

目的:分析兰州地区汉族寻常型、关节病型银屑病与HLA-DQB1*0201等位基因的相关性.方法:采用聚合酶链反应-序列特异引物(polymerase chain reaction sequence specific primers, PCR-SSP)法检测41例寻常型银屑病患者、27例关节病型银屑病患者和52名健康对照的等位基因频率.结果:寻常型银屑病患者组HLA-DQB1*0201等位基因频率较正常对照组显著增高;关节病型银屑病患者组HLA-DQB1*0201等位基因频率较正常对照组显著增高.结论:HLA-DQB1*0201等位基因可能是兰州地区汉族寻常型、关节病型银屑病的遗传标志.     

HLA-DQA1 and DQB1 alleles are associated with genetic susceptibility to psoriasis vulgaris in Chinese Han

BACKGROUND: Psoriasis vulgaris is a chronic skin disorder characterized by infiltration of inflammatory elements, keratinocyte hyperproliferation and altered differentiation. Although the pathogenesis of psoriasis is not fully understood, there is solid evidence of a susceptibility locus in the human leukocyte antigen (HLA) region. OBJECTIVES: To investigate whether HLA-DQA1 and DQB1 alleles are associated with genetic susceptibility to psoriasis vulgaris in Chinese Han. PATIENTS AND METHODS: The polymerase chain reaction-sequence-specific primer (PCR-SSP) method was used to analyse the distribution of HLA-DQA1 and DQB1 alleles in 189 patients with psoriasis and 273 healthy controls. RESULTS: The HLA-DQA1*0104 (OR = 2.33, P = 0.0001154, Pc = 2.0 x 10-3), DQA1*0201 (OR = 3.36, P < 1.0 x 10-7, Pc < 1.0 x 10-6), DQB1*0201 (OR = 1.64, P = 0.0192, Pc > 0.05) and DQB1*0303 (OR = 1.55, P = 0.0377, Pc > 0.05) alleles were more prevalent in patients with psoriasis vulgaris than in controls, and HLA-DQA1*0501 (OR = 0.30, P = 0.0000039, Pc < 4.0 x 10-5) alleles were less prevalent. The HLA-DQA1*0104 (OR = 2.42, P = 0.0001159, Pc < 2.0 x 10-3), DQA1*0201 (OR = 3.74, P < 1.0 x 10-7, Pc < 1.0 x 10-6) and DQA1*0501 (OR = 0.30, P = 0.0000374, Pc < 4.0 x 10-4) alleles were only associated with type I psoriasis. HLA-DQA1*0104 and DQA1*0201 were more prevalent in patients with or without a family history of psoriasis. However, the DQA1*0501 allele was only more prevalent in patients without a family history of psoriasis. CONCLUSION: HLA-DQA1*0104 and DQA1*0201 alleles may be psoriasis susceptibility genes or may be in close linkage with the susceptibility genes. The HLA-DQA1*0501 allele seems to have a protective effect against the development of psoriasis vulgaris in Chinese Han. There may be a difference in genetic background between psoriasis patients with and without a family history of psoriasis.     

河南地区汉族人银屑病与HLA—Cw0602等位基因的关联研究

目的：分析河南地区汉族人银屑病与HLA—Cw＊0602等位基因的相关性。方法：运用聚合酶链反应一序列特异性引物（PCR—SSP）法检测河南地区汉族人200例寻常型银屑病患者和200名健康对照的HLA—Cw＊0602等位基因频率,并分析携带该基因的银屑病患者与家族史的关系。结果：病例组HLA—Cw＊0602等位基因频率较对照组显著升高（73％VS24％,P=0．000）,但无性别差异;携带HLA—Cw＊0602等位基因的银屑病患者发病年龄早于不具有该等位基因的患者（80．2％VS28．6％,P=0．001）;有银屑病家族史患者携带HLA—Cw＊0602等位基因的频率与无银屑病家族史者差异无显著性（P=1．000）。结论：HLA—Cw＊0602等位基因与河南地区汉族人银屑病易感性高度关联。携带该等位基因的银屑病患者易为早发型,但不能确定有家族倾向性。     

HLA-DQA1*0302、DQB1*0303与新疆维吾尔族白癜风的相关性研究

目的 探讨HLA-DQA1、DQB1等位基因与新疆维吾尔族白癜风相关性。方法 聚合酶链反应-序列特异性引物（PCR-SSP）检测300例维吾尔族白癜风患者HLA-DQA1*0302、DQB1*0303等位基因。结果 与300例维吾尔族正常人对照组相比,①白癜风患者DQA1*0302（20.5%比13.83%）、DQB1*0303（30.17%比13.33%）等位基因频率显著增高（P ＜ 0.01）;②HLA-DQA1*0302、DQB1*0303等位基因频率在成人型（发病年龄 ＞ 12岁）及儿童型（发病年龄≤12岁）的白癜风患者中均增高（P ＜ 0.01）;③HLA-DQB1*0303等位基因频率在有、无家族史的白癜风患者中均增高（P ＜ 0.01）,HLA -DQA1*0302等位基因频率在无家族史病例中显著增高（P ＜ 0.01）;④白癜风组儿童型和成人型两组间比较及有、无家族史两组间比较,DQA1*0302、DQB1*0303等位基因频率差异无统计学意义（P > 0.05）。 结论 HLA-DQA1*0302、DQB1*0303等位基因可能与新疆维吾尔族白癜风相关,儿童型和成人型及有、无家族史的白癜风在其遗传背景上可能存在异质性。     

Association of HLA-DQA1 and DQB1 alleles with alolpecia areata in Chinese Hans

Accumulative evidences have shown that certain HLA loci are associated with alopecia areata (AA), but with existing differences in ethnic distribution. No report has ever been published about this in Chinese Hans. To investigate whether HLA-DQA1 and DQB1 alleles are associated with AA, and the correlation of the HLA profile with age of onset, severity, duration of current attack, recurrence and family history of AA in Chinese Hans. The polymerase chain reaction–sequence-specific primer (PCR-SSP) method was used to analyze the distribution of HLA-DQA1 and DQB1 alleles in 192 patients with AA and 273 healthy controls in Chinese Hans. The significant increased frequencies of HLA-DQA1*0104 (OR=3.38, P _c<0.001), HLA-DQB1*0604 (OR=5.17, P _c=0.006) and HLA-DQA1*0606 (OR=3.73, P _c<0.001) were observed in patients compared with controls. The DQA1*0104-DQB1*0604, DQA1*0104-DQB1*0606, and DQA1*0302-DQB1*0606 were found as high-risk haplotypes in developing AA in this study. HLA-DQA1*0104 (OR=5.31, P _c < 0.001) and -DQB1*0604 (OR=5.56, P _c=0.015) were more prevalent only in AA patients with long duration than controls. The frequencies of HLA-DQB1*0604 (OR=5.42, P _c=0.009) and -DQB1*0606 (OR=4.11, P _c<0.001) were obviously increased in patients less than 50% scalp hair loss. No locus was merely associated with early onset, severe involvement, recurrence and a positive family history of AA. This study demonstrated the positive association of HLA-DQA1 and DQB1 alleles and haplotypes with AA. There may be differences in genetic background in patients with different duration.     

泛发性脓疱性银屑病与HLA-DQB1等位基因的相关性研究

目的 探讨山东汉族人泛发性脓疱性银屑病(GPP)与HLA-DQB1等位基因的相关性。方法 运用聚合酶链反应-序列特异性引物(PCR-SSP)法,对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。结果 GPP患者组HLA-DQB10201、0603基因频率较对照组显著升高(OR=8.10,Pc=0.005;OR=5.06,Pc=0.013),而HLA-DQB10604基因频率较对照组明显降低(OR=0.08,Pc=0.039)。原有寻常性银屑病病史的GPP与HLA-DQB10201、0603强相关(OR=32.31,Pc=0.005;OR=12.42,Pc=0.005);而原无寻常性银屑病病史的GPP与HLA-DQB10602相关(OR=5.60,Pc=0.039)。结论 山东汉族GPP与HLA-DQB10201、0603等位基因高度关联,原有寻常性银屑病病史的GPP与原无寻常性银屑病病史的GPP具有遗传异质性。     

白癜风243例临床分析及与HLA-DQB1相关性研究

目的 总结和分析白癜风的临床特征及与两种HLA-DQB1等位基因的相关性,探讨白癜风的病因.方法 登记243例白癜风门诊患者的临床资料,利用聚合酶链反应-序列特异引物(PCR-SSP)法,对243例白癜风患者和250例健康人进行等位基因检测,对各项指标进行统计分析.结果 白癜风平均初发年龄男性为23.1岁,女性为23.7岁;首发类型以局限型为主,77%的节段型为儿童,家族史阳性患者发病年龄早于家族史阴性患者.白癜风与HLA-DQB1等位基因相关.结论 ①寻常型与节段型白癜风发病机制不同;②有阳性家族史者符合多基因遗传规律,有家族史和无家族史白癜风患者在其遗传背景上可能存在差异;③HLA-DQB1*0201可能是寻常型白癜风的易感基因或与易感基因相连锁.