老年胃十二指肠疾病Hp感染调查

幽门螺杆菌(Helicobacter pylori,Hp)在胃和十二指肠疾病病因学上有重要意义。本文对老年人胃和十二指肠疾病Hp感染作调查,以了解老年人该菌感染在发病学上的规律、特点、意义和青壮年的不同。 1 对象和方法 1.1 对象为住院和门诊患胃和十二指肠疾病的患者,分60岁以上(老年组)和60岁以下(青壮年组)两组,每组各     

CagA基因菌株Hp与胃、十二指肠疾病

目的通过检测胃、十二指肠疾病患者的幽门螺杆菌（Hp和CagA-Hp-IgG（细胞毒素相关蛋白抗体），探讨CagA基因菌株Hp与胃、十二指肠疾病的相关性。方法206例因胃肠症状在门诊、住院就诊者，男143例，女63例，年龄18岁～76岁．在内镜检查同时取胃粘膜组织4块，十二指肠溃疡者取距幽门1cm～2cm大弯侧，胃溃疡取溃疡边缘处，胃炎取炎症区．2块进病理检查，2块分别用尿素酶快速试验及用PCR法检测Hp，同时抽取静脉血3mL作CagA-Hp-IgG抗体检测．结果用尿素酶快速试验及PCR法检出Hp阳性者180例，男性感染率91％，女性78％，二者差异显著（P＜0．005），各年龄组构成无明显差异．两种检测方法的检出率无明显差异（P＞0．05）．检出CagA-Hp-IgG阳性者95例，在Hp感染组中阳性率89／180（50％），非感染组阳性率6／26（23％），二者差异显著（P＜0．05）．感染组CagA阳性者中，十二指肠球部溃疡44／55（80％），胃溃疡21／34（61％），慢性胃炎24／91（26％），三者差异明显（P＜0．025）．病理结果为轻、中、重度炎症CafA-Hp阳性率分别为25％，56％，70％，三者差异显著（P＜0．005）．结论CagA基因菌株Hp较CagA阴性菌株Hp具有更强的导致炎症的作用．CagA基因Hp是十二指肠球部溃疡、胃溃疡的重要发病因素．     

IL-1B及IL-1RN基因多态性与幽门螺杆菌相关的胃十二指肠疾病

白细胞介素-1(Interlukin-1,IL-1)有两种类型IL-1α和IL-1β,以IL-1β为主,主要由活化的单核巨噬细胞产生,与IL-1受体(IL-1R)结合后产生生物学作用.IL-1β是一个非常重要的前炎症细胞因子,在炎症免疫损伤机制中起着主要的调节作用,同时具有强大的抑制胃酸分泌作用,在幽门螺杆菌(Helicobacter pylori,H·py-lori)感染的胃黏膜损伤和萎缩性胃炎发展及胃癌发生中起重要作用.     

拉萨地区世居藏族胃,十二指肠疾病与幽门螺杆菌关系

拉萨地区世居藏族胃、十二指肠疾病与幽门螺杆菌关系旦增，益西加措，钟继红，德吉，巴桑我们自１９９１年９月至１９９２年１月，对拉萨地区（海拔３６５８米）因上消化道症状接受胃镜检查的门诊及住院病人进行了胃镜活检标本的Ｗａｒｔｈｉｎ－Ｓｔａｒｒｙ银染色的光学...     

幽门螺杆菌dupA基因在胃十二指肠疾病中的研究进展

幽门螺杆菌感染是一个主要的全球公共卫生问题,在发展中国家更为普遍。最近的一项系统回顾显示,全球幽门螺杆菌感染率约为48.5%,约有44亿人为幽门螺杆菌阳性。幽门螺杆菌感染是慢性胃炎和消化性溃疡的主要病因,同时也是胃癌和胃黏膜相关淋巴组织淋巴瘤的危险因素,幽门螺杆菌具有遗传多样性,不同菌株引起的临床症状存在差异,其与宿主的遗传易感性和生存环境有关,主要与细菌毒力因子有关。目前关于幽门螺杆菌dupA基因的研究取得了一定的进展,本文就幽门螺杆菌dupA基因相关研究的最新进展作一综述。     

十二指肠球部胃化生和Hp感染与胃窦部病变相关性研究

目的探讨十二指肠球部胃化生（ＤＧＭ）、Ｈｐ感染和胃窦部病变相关性,方法收集１２６例胃镜检查所见、组织病理学、Ｈｐ和胃泌酸量及胃内２４小时ｐＨ值的资料行对比研究。结果ＤＧＭ的发生率５２．３％,在反流性胃炎中６７．９％;粘膜糜烂中２０．７％;溃疡中４０．８５％,反流性胃炎与另二组差异显著（Ｐ＜０．００５）。在慢性非活动性炎症、慢性活动性炎症和胃粘膜上皮肠化生中分别为４１．１％、４５．９％和４１．８％,三组差异不显（Ｐ＞０．０５）。在轻、中和重度胃酸分泌亢进者中分别为２６．０％、６６．０％和１００％,各组间差异显著（Ｐ＜０．０５）。２４小时胃内ｐＨ＜４的时间在有、无ＤＧＭ组间无明显差异（Ｐ＞０．０５）。睡眠期间ｐＨ＞６的时间在２小时以上者ＤＧＭ明显多于无ＤＧＭ（Ｐ＜０．０５）。十二指肠球部和胃窦部Ｈｐ检出率为４１．２％和８０．９％。十二指肠球部Ｈｐ阳性者在胃窦部Ｈｐ（＋）占２７．１％;（＋＋）占８０．１％和（＋＋＋）占１００％,各组间差异显著（Ｐ＜０．０５）。结论ＤＵ患者的ＤＧＭ发生率与胃窦部的反流性胃炎、胃酸分泌亢进和Ｈｐ感染等病有相关性。     

川藏高原民族胃,十二指肠疾病特点与胃幽门螺杆菌感染

１９９４年１２月至１９９６年７月 ,我们对５８２例有上消化道症状的高原人进行胃镜检查 ,以探讨高原地区汉族及少数民族胃．十二指肠疾病的特点与胃幽门螺杆菌感染的关系。一、临床资料 :５８２例患者均符合下列条件 :１．海拔２０００～５０００米高原地区少数民族居民及移居高原持续生活３年以上汉族居民 ;２．有上消化道症状未接受抗幽门螺杆菌菌 (Ｈｐ）治疗 ;３．受观察者离开高原后３天内接受胃镜检查。本组５８２例中藏族２６０例 (４４．６７ % ) ,羌族１４４例(２４．７４ % ) ,汉族１０６例 (１８．２１ % ) ,彝族７２例 (１２…     

含cagA基因幽门螺杆菌感染与胃、十二指肠疾病的相关性研究

幽门螺杆菌（Ｈｐ）感染是引起胃、十二指肠疾病的重要病因。Ｈｐ感染可持续数十年甚至终生,但是,Ｈｐ感染人群大多无症状,只有少数人可致病,这主要是由于Ｈｐ菌株间毒力不同所致。含ｃａｇＡ基因（细胞毒素相关蛋白的基因）的菌株,无论表型和基因型都与无ｃａｇＡ基...     

幽门螺杆菌vacA基因型及其表达产物与胃十二指肠疾病关系的研究

目的 探讨幽门螺杆菌ｖａｃＡ基因型及其表达产物－ＶａｃＡ与胃十二指肠疾病之间的关系。方法 用聚合酶链反应技术测定６２株从慢性胃炎,消化性溃疡和胃癌患者中分离获得的幽门螺杆菌（Ｈｐ）菌株的ｖａｃＡ基因型,用Ｈｅｌａ细胞测定Ｈｐ菌株体外ＶａｃＡ活性。     

环氧合酶-2表达与幽门螺杆菌相关性胃十二指肠疾病的研究

目的　探讨环氧合酶 2表达与幽门螺杆菌Helicobacterpylori ,H .pylori相关性胃十二指肠疾病的关系 ,并通过抗菌治疗评价根除H pylori感染对胃窦黏膜中COX 2表达的影响。方法　用免疫组化方法半定量检测 2 64例经胃镜和组织病理学检查患有十二指肠球部溃疡、胃溃疡、复合性溃疡、胃癌、单纯性慢性胃炎及胃黏膜正常者的胃窦黏膜COX 2蛋白的表达 ,比较H pylori感染与非感染者之间的差异。对检出的 3 5例H pylori的单纯慢性胃炎进行H pylori抗菌根除治疗 ,比较根除前后胃窦黏膜COX 2蛋白的表达变化。根据 2 0 0 0年 5月全国慢性胃炎研讨会共识意见 (江西 井冈山 )对胃黏膜炎症、活动性、异型增生、肠化生和H pylori密 ,度进行半定量测定。结果　胃黏膜表面上皮、腺上皮细胞和固有层间质细胞的浆中可见COX 2蛋白表达 ,但阳性染色细胞多集中在表层上皮。 2 53例中 ,14 3例H pylori者 (56 52 % )COX 2平均阳性细胞率显著高于 110例H pylori者 (43 48% ) ,(P =0 ) ,各疾病组H pylori患者的COX 2平均阳性细胞率均显著高于H pylori者 (P =0 ) ,各疾病组H pylori患者COX 2平均阳性细胞率也均显著高于正常对照组 (P <0 0 5)。 2 7例H pylori根除后的胃黏膜COX 2平均阳性细胞率明显下降 (P =0 ) ,但仍明显高于正     

Gastric metaplasia and duodenal ulcer disease in children infected by Helicobacter pylori.

BACKGROUND--Helicobacter pylori infection of the gastric mucosa is vital in the pathogenesis of duodenal ulcer disease. H pylori will only colonise gastric epithelium and its association with duodenal disease is therefore not easily explained. AIMS--To determine if gastric metaplasia in the duodenum increases the risk of duodenal ulcer disease in children infected with H pylori. PATIENTS--All children undergoing upper endoscopy over a 20 month period in a children's hospital in Ireland. METHODS--Two biopsy specimens were obtained from the antral mucosa and two from the first part of the duodenum. One antral biopsy specimen was used in a rapid urease test (Clo Test). Biopsy sections were stained with haematoxylin and eosin and also with cresyl violet for identification of H pylori. Periodic acid Schiff (PAS) stain was performed to identify areas of gastric metaplasia. RESULTS--Gastric and duodenal biopsy specimens were obtained from 148 patients (M:F 1:2:1). Twenty five children (17%) had H pylori positive gastritis. Thirty four children (23%) had gastric metaplasia in the duodenum. Nine per cent of children under the age of 8 years had gastric metaplasia compared with 38% in those 12 years of age or over (p < 0.005). Seven children had duodenal ulcer disease. Gastric metaplasia was present in six of seven (86%) children with duodenal ulcer disease compared with 28 of 141 (20%) without ulceration (p < 0.001). While both H pylori and gastric metaplasia were each significant risk factors for duodenal ulcer disease, the combined presence of both factors was associated with a pronounced increase in duodenal ulcer disease. Duodenal ulcer disease occurred in over 50% of children with both H pylori infection and gastric metaplasia. In contrast duodenal disease did not occur in children (0 of 100) when both were absent. CONCLUSION--The presence of gastric metaplasia in the duodenum is the major risk factor for duodenal ulcer disease in patients colonised by H pylori.     

胃十二指肠疾病幽门螺杆菌检出的意义

目的:研究幽门螺杆菌(Hp)与消化性溃疡及慢性胃炎致病的关系。方法:因胃十二指肠疾病而做胃镜的患者,采取胃粘膜进行组织快速检菌,一分钟尿素酶(1min UT)试验和血清间接免疫荧光法进行检菌分析。结果:对5000例胃及十二指肠疾病Hp的组织检出率为88.1％。1min UT阳性率为62.9％,两种检菌法有显著差异(P<0.01)。十二指肠溃疡Hp检出为96.0％,胃癌和萎缩性胃炎Hp感染率为50.0％和59.2％,而1min UT只有41.5％阳性率。结论:胃癌和萎缩性胃炎均有粘膜层萎缩,腺体减少、粘液分泌功能降低,Hp茵不能适应强酸环境下生存。十二指肠溃疡和慢性胃炎多伴引起幽门口水肿和幽门变型,胃排空减缓。是导致Hp茵高检出率的重要因素。     

蒙古沙土鼠不同幽门螺杆菌菌株感染相关性胃病的研究进展

幽门螺杆菌(Helicobacter pylori,H.pylori)感染在世界范围内高发,他定植于人胃黏膜,导致慢性胃炎及胃癌的发生.蒙古沙鼠(mongolian gerbil,MG)很少患自发性胃炎,且不是H.pylori 的自然宿主.人工接种H.pylori后,蒙古沙鼠患H.pylori相关性胃病与胃病患者最相似...     

蛋白质二硫键异构酶在幽门螺杆菌感染者胃黏膜中的表达及意义

目的：了解蛋白质二硫键异构酶(protein disulfide isomerase,PDI)在幽门螺杆菌(H pylon)感染人胃黏膜组织中的表达情况．方法：分别应用半定量RT-PCR方法及Westem blot方法检测感染(n=32)与未感染(n=28)H pylori的人胃黏膜组织中PDI mRNA及蛋白的表达情况．结果：未感染组PDI mRNA及蛋白表达量分别为0．5704±0．0794,0．5198±0．0379,感染组PDI mRNA及蛋白表达量分别为1．0642±0．1533,0．8252±0．0321：两组相比差异显著(P＜0．01)．结论：正常情况下胃黏膜组织有PDI mRNA的表达及蛋白的合成,H pylori感染可使胃黏膜增加PDI mRNA的表达及蛋白的合成．     

Dental caries is common in Finnish children infected with Helicobacter pylori.

Childhood factors such as low socioeconomic status are risk factors for Helicobacter pylori infection and Streptococcus mutans-related dental caries. We examined whether H. pylori infection and dental caries are present today in the same group of children examined previously. We reviewed the public dental health service files of 21 H. pylori-positive children (upper gastrointestinal endoscopy at a median age of 13.5 y) and 27 H. pylori-negative children (endoscopy at a median age of 12.5 y) examined during 1995-98 at the Helsinki University Central Hospital, Finland. All H. pylori-positive children had experienced dental caries in their primary or permanent teeth or in both whereas among H. pylori-negative children the respective proportion was 70% (p < 0.01). At the age of 7 y, 18% (3/17) of the H. pylori-positive children had experienced caries in permanent teeth as compared to 0% among H. pylori-negative children (0/24; p < 0.05). At the age of 12 y, H. pylori-positive children had more decayed, missing or filled permanent teeth than H. pylori-negative children (80% vs. 38%; p < 0.05). Although a causal relationship between H. pylori and dental caries is unlikely, it is possible that H. pylori-infected children have an increased risk of other health problems, such as dental caries, for which proper treatment is needed.     

Prevalence of Helicobacter pylori infection in duodenal ulcer and gastro‐duodenal ulcer diseases in Taiwan

Background and Aim: The prevalence of Helicobacter pylori‐negative duodenal ulcer (DU) is increasing in Western countries but is rare in Japan. We aimed to examine the prevalence of H. pylori infection and the characteristics in DU and gastro‐duodenal ulcer (GDU) diseases in Taiwan. Study: All patients with an endoscopic diagnosis of DU or GDU from September 2003 to May 2004 at Taipei Veterans General Hospital were included. Rapid urease test was done for all patients, while urea breath test was carried out on those with negative rapid urease tests. A patient was considered infected if either test was positive. Results: The prevalence of H. pylori was 88.7% (555/626) in DU and 90.5% (95/105) in GDU patients. There was no difference in sex and prevalence of H. pylori between the two groups but age was higher in the GDU patients (60.1 ± 15.5 vs. 55.4 ± 15.5, P = 0.005). Of H. pylori‐negative DU patients, 28.2% (20/71) reported using non‐steroidal anti‐inflammatory drugs (NSAIDs)/aspirin, which were used by all 10 H. pylori‐negative GDU patients (100%) (P < 0.001). There was no difference in sex and age between H. pylori‐positive and negative DU patients. The prevalence rate of H. pylori in DU was not statistically different among outpatients, inpatients, and physical check‐up subjects (86.8% vs. 93.3% vs. 90.7%, P = 0.163). Conclusion: The prevalence of H. pylori infection in DU appears to be decreasing in Taiwan. Thus, eradication therapy without confirming the presence of H. pylori in DU patients cannot be recommended. NSAIDs/aspirin is the major risk factor for H. pylori‐negative DU patients, especially those with co‐morbid gastric ulcer.     

Bacterial flora concurrent with Helicobacter pylori in the stomach of patients with upper gastrointestinal diseases

AIM: To investigate the non-Helicobacter pylori (H. pylori) bacterial flora concurrent with H. pylori infection.METHODS: A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H. pylori bacterial isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).RESULTS: A total of 201 non-H. pylori bacterial isolates were cultivated from 67 (65.0%) of the 103 gastric samples, including 153 isolates identified successfully at species level and 48 at genus level by MALDI-TOF MS. The dominant species were Streptococcus, Neisseria, Rothia and Staphylococcus, which differed from the predominantly acid resistant species reported previously in healthy volunteers. The prevalence of non-H. pylori bacteria was higher in non-ulcer dyspepsia group than in gastric ulcer group (100% vs 42.9%, P < 0.001). Six bacterial species with urease activity (Staphylococcus epidermidis, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Brevibacterium spp. and Klebsiella pneumoniae) were also isolated.CONCLUSION: There is a high prevalence of the non-H. pylori bacteria concurrent with H. pylori infection, and the non-H. pylori bacteria may also play important as-yet-undiscovered roles in the pathogenesis of stomach disorders.