Investigations On A Cell Culture Medium For Storage And Transportation Of Avulsed Teeth

Non-physiologic storage of avulsed teeth leads to a high incidence of root resorption, resulting in poor prognosis. This study investigated the suitability of specially composed cell culture media for storage of extracted teeth for up to 48 hours. Autoradiographic investigations revealed that the proliferative activity of periodontal ligament (PDL) cells of teeth stored in cell culture medium for up to 48 hours increased with storage time. Studies on proliferation of PDL cells after storage of teeth in different media for up to 24 hours demonstrated that the proliferative activity is dependent on the composition of the medium. Immunohistochemical investigations with markers for cell proliferation revealed that pulp cells of extracted immature teeth show numerous proliferations after storage for up to 24 hours in a special cell culture medium but few proliferations after storage in Hanks Balanced Salt Solution (HBSS). The investigations indicate that a special cell culture medium can preserve cell viability of PDL cells adhering to extracted teeth for at least 48 hours. The in vitro results are confirmed by a case presented: After storage of two upper central incisors for 36 hours in the cell culture medium the teeth could be successfully reimplanted after extraoral insertion of titanium posts into the root canal (auto-alloplastic reimplantation). Clinical and radiological follow-up examinations for 12 months revealed normal periodontal healing.     

外伤性牙脱位治疗的临床分析

目的:研究外伤性脱位前牙的临床治疗。方法:对142颗不同类型的脱位前牙治疗方法和临床疗效进行分析。结果:脱位前牙再植后牙髓血管再生,根尖完全形成,牙髓存活率达8.5%;根管治疗率达79.6%,其中部分脱位牙为76.7%,完全性脱位牙为90.4%;脱位牙的牙根吸收率45.1%,其中,根管治疗前为39.4%,根管治疗后为5.7%。结论:牙髓血管再生是牙再植术成功的关键,而根管治疗是脱位前牙治疗的重要手段,早期根管治疗可预防或减少脱位牙牙根的吸收。     

恒牙全脱位再植的研究现状

恒牙外伤常导致牙齿全脱位,再植术是恒牙全脱位的首选治疗方法。理想的预后是实现牙周功能性愈合,若牙根尖未完全封闭,尚有牙髓再生的可能。恒牙再植术的预后与很多因素有关,如牙齿脱离牙槽窝的时间、牙根的保存以及牙齿的储存介质等,目前临床治疗效果尚不理想。本文就恒牙全脱位再植的研究现状作一综述。     

Incidence of Root Resorption after the Replantation of Avulsed Teeth: A Meta-analysis

Introduction

An avulsion injury is a serious trauma to pulp and periodontal tissues. After avulsion and replantation, teeth are at risk of infection and root resorption, which may affect treatment outcome and survival rate. Thus, the purpose of this systematic review was to evaluate the incidence of root resorption after the replantation of avulsed teeth.

The Use of Histidine-tryptophan-ketoglutarate Solution as a New Storage Medium for the Avulsed Tooth

IntroductionHistidine-tryptophan-ketoglutarate (HTK) is a preservation solution used for organ transplantation. The physiological pH and osmolality of this solution are known to facilitate cell proliferation and cell membrane stabilization. The purpose of the present study was to investigate the efficacy of several concentrations of HTK solution as a storage medium for avulsed teeth.MethodsCultured human periodontal ligament cells were stored in different concentrations of HTK solutions. After 1, 3, 6, 12, 24, 48, and 72 hours, cell viability was assessed using the Cell-Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan) and LIVE/DEAD (Invitrogen, Carlsbad, CA) assay. Cell response of the most effective concentrations of HTK solution were further analyzed by gene expression profiling, and their cell viability was compared with other storage media.ResultsThe highest cell viability was observed in 50% HTK solution in various concentrations of HTK solution (P < .05). In periodontal ligament cells stored in 50% HTK solution for 3 hours, the expression of genes related to angiogenesis, the inflammatory response, and cell proliferation was increased compared with the control. Compared with other storage media, the highest cell viability was observed in 50% HTK solution.ConclusionsOur study suggests that 50% HTK solution containing cell culture medium represents a suitable storage medium for avulsed teeth.     

45例外伤性脱位牙的诊治体会

牙脱位是口腔内科常见牙体损伤性疾病之一，施行再植术是保持牙列完整，恢复咀嚼、发音和美观的关键。现介绍45例外伤性脱位牙施行再植的治疗情况，经过3a以上的随诊观察，疗效满意。1病例与方法1.1临床资料共诊治脱位牙45例，男32例、女13例。年龄12～45岁，以22岁以下居多，26例。牙齿离体时间，最短20min，最长72h，其中离体时间在1h以内的为39例。由于外伤程度不同，脱位牙有不同程度的牙周膜附着。有5例患者有牙槽突骨折伴牙龈撕裂。1.2治疗方法对于离体1h以内的牙齿，先不做根管治疗，用生理盐水冲洗牙齿表面的污物，注意保持牙周膜。…     

Influence of Atopy in the Outcome of Avulsed and Replanted Teeth during 5 Years of Follow-up

Introduction

The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents play an active role in root resorption by inducing specific and non-specific immune responses; however, little information exists regarding the influence of the acquired immune response on replantation. Therefore, the aim of this study was to evaluate the 5-year outcome of replantation in non-atopic and atopic patients.

上颌前牙埋伏阻生的X线分析

目的　总结上颌前牙埋伏阻生的X线影像特点。方法　收集 2 92例患者上颌前牙埋伏阻生的X线片 ,观察埋伏阻生牙的牙位、位置高低、牙冠方向及周围组织情况。结果　 2 92例 387枚阻生牙 ,其中 2 92枚颌骨内埋伏 ;6 7枚牙根弯曲 ;5 6枚埋伏阻生牙异位或错位。埋伏阻生的相关情况主要有 :冠部有致密骨壁线包绕 2 11枚 ;萌出间隙不足 94枚 ;受多生牙阻挡 5 2枚。埋伏方向 :平行 15 9枚 ;倒置 32枚 ;斜位 14 0枚和水平 4 7枚。结论　埋伏阻生牙的冠周有骨壁线包绕 ,又不能正常萌出应视为含牙囊肿或囊样变。判断埋伏牙阻生方向应从六面的三维视角进行定位。?     

Avulsed permanent teeth: a review of the literature and treatment guidelines

Abstract Dental trauma represents one of the few situations where dentists are called upon to make unscheduled diagnostic and treatment decisions in an area that is outside their routine experience. Since patients who sustain an avulsion present infrequently, except in child-oriented or emergency-based practices, clinicians often make diagnostic and management decisions based upon their previous rare treatment experiences. Clinicians also rely on published guidelines for this aspect of their practice and expect these standards to be up-to-date and based on current research information. None of the current protocols has been tested by a prospective longitudinal outcome study in humans. Nevertheless, current guidelines have become the standard for clinical practice around the world. An effort must be made to develop treatment protocols that are based upon the biological mechanisms that underlie periodontal wound healing.     

牙纵裂治疗效果与失败原因分析

目的 总结1990～2002年12a中临床治疗牙纵裂的疗效和失败原因。方法 257例牙纵裂患者选用常规牙周治疗，去牙垢、牙石、冲洗上药，拴丝结扎。行根管治疗或塑化治疗，完成充填再行全冠修复。结果 257例中，进行多次追踪观察均在1a以上，最长12a，疗效满意，成功225例(87．5％)，失败32例(12．5％)。结论 牙纵裂只要采取及时有效的治疗，息牙可以保留，并能承担咀嚼压力，有利于保持牙弓的完整性。     

青少年恒牙色泽测试分析

目的 :牙齿的色泽有增龄性变化 ,本文目的是要了解青少年恒牙的颜色特点 .方法 :先选用 Vita与Shou比色板进行目测 ,再用 Shade Eye牙科颜色测定器进行仪器测试 .结果 :三种测试法都显示 A系列占大多数 ,且 A2 居多 .牙色测定器显示色相偏红 ,亮度较亮 ,饱和度适中 .结论 :青少年恒牙颜色较淡 ,且较透亮 .     

乳牙牙体元素含量的光谱分析

本研究采用原子发射光谱仪,定量分析了健康.未龋和龋齿3组共46颗乳牙牙体组织的18种元素含量,对不同乳牙的元素测定结果作了统计分析.结果表明,与健康乳牙相比,龋齿乳牙钙、砷含量高,而铅、锌含量低;与健康乳牙和恒牙相比,钠、铁含量高,镁、铜、铅、锌等元素含量低.     

一种新型的牙齿根管长度测量仪

用牙科发声探针测量牙齿根管长度,操作简便,准确度高.本文介绍了YⅡ型牙科发声探针的设计原理及工作过程,并给出了整机的电路原理图.     

Outcome of Periapical Surgery in Molars: A Retrospective Analysis of 424 Teeth

IntroductionThe objective of this retrospective study was to assess the outcome of periapical surgery in a large number of molars in order to identify possible variables that might affect the outcome.MethodsThe healing outcome of patients undergoing periapical surgery of molars from October 1999 to October 2019 was retrospectively evaluated. Outcome was dichotomized into “healed” and “nonhealed” using well-established clinical and radiographic healing criteria. The potential influence of patient-, tooth-, and treatment-related parameters on the healing outcome was analyzed.ResultsA total of 424 molars in the same number of patients (45.5% male and 54.5% female) were evaluated. Three hundred seventy-two molars were classified as healed (87.7%). Three significant outcome predictors were identified: 1-year follow-up versus >1–5 years, >5–10 years, and >10 years (95.3% vs 82.2%, 76.3%, and 76.5% healed, respectively; P < .0001); root end filling material with bioceramic root repair material versus mineral trioxide aggregate (96.9% vs. 86.3% healed, respectively; P = .001); and preoperative evaluation based on cone-beam computed tomographic imaging versus 2-dimensional radiography (90.2% vs 81.4% healed, respectively; P = .02). Sex, age, tooth location, type of restoration, attachment level, presence of a post, quality of the root canal filling, technique of root end preparation, administration of antibiotics, and type of surgery had no significant impact on the healing outcome.ConclusionsThe healed rate for the concave (Retroplast) and cavity (mineral trioxide aggregate, SuperEBA [Staident International, Staines, UK], and bioceramic root repair material) root end preparation technique over all follow-up periods was 84% and 88.5%, respectively. The follow-up period, root end filling material, and preoperative evaluation based on cone-beam computed tomographic imaging had a significant influence on the healing outcome.     

The Science of Anterior Teeth Selection for a Completely Edentulous Patient: A Literature Review

Esthetic replacement and physiological tooth arrangement made the complete denture biologically compatible and desirable. Proper placement of tooth should be functional and esthetically pleasing to enhance the psychology of the patient. This article reviews the evolution of concepts for teeth selection and the recent techniques employed for selecting anterior teeth for complete dentures.