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首杞补肾口服液对小鼠免疫功能的影响 总被引:2,自引:0,他引:2
目的:观察首杞补肾口服液对小鼠免疫功能的影响。方法:观察首杞补肾口服液对醋酸氢化考的松所致免疫抑制小鼠脾脏淋巴细胞转化、自然杀伤(NK)细胞活性、产生溶血素能力的影响以及对环磷酰胺所致免疫抑制小鼠腹腔巨噬细胞吞噬功能的影响。结果:首杞补肾口服液对醋酸氢化考的松所致免疫抑制小鼠脾脏淋巴细胞转化有明显的纠正作用,使NK细胞活性恢复更为显著,产生溶血素的能力明显提高,以及可使低下的吞噬功能明显恢复。结论:首杞补肾口服液对免疫抑制模型(醋酸氢化考的松或环磷酰胺所诱导)小鼠的细胞免疫(淋巴细胞转化、NK细胞活性)、体液免疫(溶血素)和非特异性免疫(巨噬细胞吞噬实验)等功能有明显的治疗作用,说明首杞补肾口服液对免疫功能低下有显著的调节作用,能够增强机体的抵抗力。 相似文献
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目的:观察护肝素片对小鼠免疫功能的影响。方法:用正常小鼠作为实验动物。结果:护肝素片能显著提高小鼠腹腔巨噬细胞吞噬百分率和吞噬指数,促进溶血素和溶血空斑的形成和淋巴细胞的转换。结论:护肝素片有好的免疫兴奋作用。 相似文献
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目的:观察马齿苋提取物对正常小鼠免疫功能的影响.方法:观察马齿苋提取物对小鼠腹腔巨噬细胞吞噬功能、溶血素形成、溶血空斑形成及淋巴细胞转化功能的作用.结果:马齿苋提取物可显著提高小鼠腹腔巨噬细胞的吞噬百分率和吞噬指数;促进溶血素及溶血空斑的形成;促进淋巴细胞的转化.结论:马齿苋提取物有提高免疫功能的作用. 相似文献
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目的探讨蜂王浆对环磷酰胺(CTX)所致免疫低下小鼠免疫功能的影响。方法建立小鼠免疫损伤模型,同时灌胃给予不同剂量蜂王浆连续7 d,以小鼠巨噬细胞吞噬功能、胸腺脾脏指数、迟发型超敏反应、血清溶血素水平为测定指标。结果蜂王浆3.06,6.12 g.kg-1剂量组能够明显增强小鼠巨噬细胞吞噬功能(P<0.05),同时,对小鼠胸腺脾脏指数具有一定的保护作用,并且能明显增加小鼠迟发型超敏反应,提高小鼠的血清溶血素含量。结论蜂王浆对CTX所致免疫低下小鼠免疫功能具有一定的增强作用。 相似文献
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[目的]探讨人参皂甙Rg3对H22荷瘤小鼠免疫微环境的影响。[方法]60只雌、雄昆明种小鼠随机分为生理盐水组(A组,5mL/kg)、华蟾素组(B组,5mL/kg)、低剂量人参皂苷Rg三组(C组,0.2mg/kg)、中剂量人参皂苷Rg3组(D组,1.0mg/kg)、高剂量人参皂苷Rg3组(E组,2.0mg/kg),每组12只,H22荷瘤模型建立后24h开始灌胃给药治疗,1次/d,连续8d,观察小鼠初始重量、处死重量、瘤体重量、CD3+、CD4+、CD8+、CD4+/CD8+等指标变化。[结果]各组荷瘤小鼠初始重量比较无统计学意义(P>0.05),处死重量、瘤体重量、B组、C组、D组、E组与A组比较分别比较差异均具有统计学意义(P<0.05);抑瘤率、CD3+、CD4+、CD4+/CD8+、存活小鼠生存时间,B组、C组、D组、E组均高于A组,生存时间以D组最长。[结论]不同剂量人参皂甙Rg3改善了H22荷瘤小鼠肿瘤所致的免疫抑制,其中以人参皂甙Rg3 1.0mg/kg剂量抑瘤效果最佳,小鼠生存时间最长,为临床应用提供了试验依据。 相似文献
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大蒜素对小鼠免疫功能的影响 总被引:21,自引:1,他引:21
研究了大蒜素对小鼠免疫功能的影响。结果表明大蒜素具有明显啬强小鼠腹腔Mφ的吞噬功能,提高T淋巴细胞转化率及啬强NK细胞的活性。大蒜素具有明显啬强机体细胞免疫功能的作用。 相似文献
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Ginsenosides, the active component of Panax ginseng, have been shown to evidence a variety of biological activities associated with hyperglycemia, obesity and type 2 diabetes mellitus. This study evaluated the effects of the ginsenosides, Rg3 and Re, on glucose uptake and the glucose transport system in mature 3T3‐L1 cells. The results demonstrated that the glucose uptake of ginsenosides Rg3 and Re at concentrations of 1–10 µM significantly increased by approximately ~10% and ~12%, respectively. Furthermore, the glucose transporter 4 (GLUT4) mRNA expression of ginsenosides Rg3 and Re at 10 µM was increased by approximately ~1.73 and 1.43 fold, respectively. It was further confirmed in a series of experiments that ginsenosides Rg3 and Re stimulated the mRNA expression of insulin receptor substrate (IRS‐1) and the expression of phosphatidylinositol 3‐kinase (PI3K)‐110α protein, which is involved in downstream events in the insulin signaling pathway. These findings demonstrate that ginsenosides Rg3 and Re may stimulate glucose uptake via the PI3K pathways involving IRS‐1. Further, our results suggest that both of these ginsenosides might prove useful as effective antidiabetic and antihyperglycemic agents. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
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Yuxia Xu Peng Zhang Chu Wang Ye Shan Dandan Wang Fenglei Qian Mengwei Sun Cuiqing Zhu 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Ginsenoside Rg3 has shown multiple pharmacological activities and been considered as one of the most promising approaches for fatigue treatment. However, little is known about the cellular and molecular mechanisms of Rg3 on anti-fatigue and the effect of Rg3 on dopaminergic system has not been reported yet. The major aim of this study is to investigate the effect of Rg3 on TH expression and the related biochemical parameters, such as PKAα, ERK1/2, Akt and α-synuclein in brain of fatigue rats.Materials and methods
Weight-loaded forced swimming was performed to establish an animal model of fatigue. Rg3 (10 mg/kg, 50 mg/kg and 100 mg/kg) was intragastrically administrated before swimming. The effect of Rg3 on the expression and phosphorylation of TH and TH-related proteins in fatigue rats or in SH-SY5Y cells was assessed with western blotting. HPLC was used to examine the level of DA and DOPAC in the fatigue rats tissues.Results
TH and phosphorylated TH were decreased in different brain regions of which ventral midbrain were less affected in weight-loaded forced swimming rats. Pretreatment with Rg3 significantly suppressed fatigue-induced decrease expression of TH and TH phosphorylation. Also treatment with Rg3 reversed the decrease expression of PKAα as well as the phosphorylation of ERK1/2 and Akt which were induced by weight-loaded forced swimming. Moreover, weight-loaded swimming could induce the increase expression of α-synuclein in hippocampus and midbrain, while suppressed α-synuclein expression in striatum and prefrontal cortex. Furthermore, Rg3 could induce the increase of TH expression and phosphorylation which was accompanied with elevated expression and phosphorylation of related kinase proteins in vitro, while the inhibitors of kinase proteins could suppress these effects of Rg3. In addition, HPLC results showed that Rg3 could reverse the weight-loaded swimming-induced increase of DOPAC/DA ratio.Conclusion
Our data suggest that fatigue can induce the decrease of DA which might partially result from the change of TH expression and phosphorylation, and Rg3 can reverse these fatigue-induced changes. The underling mechanisms may include the activity changes of PKAα, ERK1/2, Akt and α-synuclein. 相似文献16.
人参皂甙Rb1和Rg1对幼小鼠性腺和副性腺的影响 总被引:6,自引:2,他引:6
幼年小鼠皮下注射人参皂甙Rb16mg/kg、Rg125和50mg/kg连续14天,结果Rb1能能使正常雌性小鼠子宫及雄性小鼠精囊明显增重,Rg1也可使正常雄性小鼠精囊明显增重,但却不能阻止去势小鼠副性腺的萎缩。提示人参皂甙本身无性激素样作用,对副性腺的增重作用需要有性腺的存在 相似文献
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目的为了提高人参皂苷Rg3的生物利用度和靶向性,以卵磷脂和胆固醇为载体材料,研究人参皂苷Rg3脂质体的处方和制备工艺。方法采用薄膜超声分散法制备脂质体,HPLC测定人参皂苷Rg3的含量。以包封率为指标,采用单因素和正交试验法优选处方和工艺。结果人参皂苷Rg3脂质体的最佳处方和工艺条件为:以氯仿-乙醇(1∶1)为溶剂,卵磷脂、胆固醇、人参皂苷Rg3的质量比为4∶2∶1,载药温度为50℃,PBS缓冲溶液的p H值为7.5。制备的脂质体平均粒径为111.8 nm,包封率为87.85%。结论该工艺方法简便、稳定可行,适用于人参皂苷Rg3脂质体的制备。 相似文献
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Eun-Jin Kim Il-Hoon Jung Thi Kim Van Le Jin-Ju Jeong Nam-Jae Kim Dong-Hyun Kim 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Panax ginseng (family Araliaceae) is traditionally used as a remedy for cancer, inflammation, stress and aging.Aim of study
To explore whether ginsenosides Rg5 and Rh3, the main constituents of heat-processed ginseng (the root of Panax ginseng), could protect memory deficit.Materials and methods
We isolated ginsenosides Rh3 and Rg5 from heated-processed ginseng treated with and without human feces, respectively. Then we investigated their protective effects on memory impairment using the passive avoidance, Y-maze and Morris water maze tasks in mice. Memory deficit was induced in mice by the intraperitoneal injection of scopolamine.Results
Ginsenosides Rg5 or Rh3 increased the latency time reduced by scopolamine in passive avoidance test. Treatment with ginsenoside Rg5 or Rh3 significantly reversed the lowered spontaneous alteration induced by scopolamine in Y-maze task. Ginsenoisde Rg5 or Rh3 (10 mg/kg) significantly shortened the escape latencies prolonged by treatment with scopolamine on the last day of training trial sessions in Morris water maze task. Furthermore, ginsenosides Rg5 and Rh3 inhibited acetylcholinesterase activity in a dose-dependent manner, with IC50 values of 18.4 and 10.2 μM, respectively. The inhibitory potency of ginsenoside Rh3 is comparable with that of donepezil (IC50=9.9 μM). These ginsenosides also reversed hippocampal brain-derived neurotrophic factor (BDNF) expression and cAMP response element-binding protein (CREB) phosphorylation reduced by scopolamine. Of them, ginsenoside Rh3 more potently protected memory deficit.Conclusions
Ginsenoside Rg5 and its metabolite ginsenoside Rh3 may protect memory deficit by inhibiting AChE activity and increasing BDNF expression and CREB activation. 相似文献19.
目的:研究20(S)人参皂苷Rg3的高效液相色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法:利用HPLC方法,采用梯度洗脱,测定了10批20(S)人参皂苷Rg3样品。采用HPLC-MS和对照品相结合的方法,确定主峰和主要共有峰的归属。结果:20(S)人参皂苷Rg3指纹图谱有5个共有峰,主峰为20(S)人参皂苷Rg3,3号和4号峰是20(S)人参皂苷-Rg3的双键位置异构体,5号共有峰为人参皂苷Rg5。结论:为20(S)人参皂苷Rg3的质量控制方法提供了特征性、专属性强的指纹图谱。 相似文献
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Elma Z. Tchilian Ilian E. Zhelezarov Christina I. Hadjiivanova 《Phytotherapy research : PTR》1991,5(1):46-48
The effect of in vivo administration of ginsenoside Rg1 from Panax ginseng on insulin binding in liver and brain membranes of mice was studied. Ginsenoside Rg1 at a dose of 10mg/kg significantly increased [125]insulin binding in both tissues. The increase in insulin binding was related to an increase in the number of insulin receptors rather than to a change in the receptor affinity. 相似文献