Antifungal and antibacterial activities of different extracts of Harungana madagascariensis stem bark

The antifungal and antibacterial effects of the stem bark extracts of Harungana madagascariensis Lam. ex Poir. (Clusiaceae) were examined against nine microbial pathogens causing infections in both man and animals. Hexane (H), dichloromethane (D), chloroform (C), ethyl acetate (E), acetone (A), methanol (M), and water (W) extracts were tested in vitro through bioautography and minimum inhibitory concentrations (MIC) determination using the serial micro-dilution assays. Bioautographic results revealed the presence of eight different spots. Extract A exhibited the lowest MIC of 0.04?mg/mL against Microsporum canis, while water extract (W) and methanol (M) showed the highest MIC of 2.5?mg/mL against at least one of the tested fungi when compared to amphotericin B with 0.0625–1?g/mL. Sporotrichum schenckii was the most susceptible fungal pathogen with average MIC of 0.06?mg/mL, while the acetone extract (A) was the most active against three fungal organisms when compared with other extracts. Similarly, extracts D, C, E and A exhibited very high activity with low MIC values of 0.156–0.62?mg/mL, while M and W gave the highest values of 0.31–2.5?mg/mL on bacterial pathogens as compared to gentamicin (0.02–0.62 8?g/mL). The dichloromethane extract is the most active against bacteria with average MIC of 0.19?mg/mL, while Staphylococcus aureus is the most sensitive organism; that shows susceptibility at an average MIC of 0.34?mg/mL. These results provide promising information for the potential use of the crude extracts from the stem-bark of H. madagascariensis in the treatment of bacterial and fungal infections similar to what was obtained in the leaves.     

Antifungal Properties of Some Components of Senna Alata Flower

Antifungal activity of some components of Senna alata flower was examined against five fungi. The methanolic crude extract and the partially purified fractions were both active against standard strains of Aspergillus niger, Geotricum candidum, and Candida utilis, and local isolates of Aspergillus brevipes and Penicillium species, but at different concentrations. The minimum inhibitory concentration of the methanolic crude extract was low for all the fungal strains except Aspergillus niger with 5.0 mg/mL concentration, while the minimum inhibitory concentration of partially purified components (C and F) ranged between 0.312 mg/mL for extract F against Penicillium species and 2.5 mg/mL for extract C against Aspergillus niger, Penicillium species and Candida utilis. The minimum fungicidal concentration of the crude methanolic extract was generally higher than 5 mg/mL for most of the fungi but the partially purified extract exhibited fungicidal concentrations ranging from 2.5 mg/mL to 5.0 mg/mL. Antifungal properties against mycelial growth was also demonstrated by all the extracts against the tested strains, with the crude methanolic extract losing its activity after 48 hours. The partially purified Senna alata extracts exhibited a relatively high antifungal activity against mycelial growth with total suppression of sporulation for four days at a concentration of 2 mg/mL, while preventing fungal growth after the seventh day.     

Antibacterial activity of wild Xylaria sp. strain R005 (Ascomycetes) against multidrug-resistant Staphylococcus aureus and Pseudomonas aeruginosa

There is a growing need for new and effective antibiotic agents due to the recent emergence of life-threatening, multidrug-resistant bacterial infections such as methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. In the present study, the antimicrobial potential of mushroom was investigated against multidrug-resistant bacterial strains. The mushroom was identified as Xylaria sp. strain R005 based on the morphological characteristics and confirmed by 18S ribosomal RNA sequence comparisons. The crude ethyl acetate extracts of culture filtrate and fruiting bodies of Xylaria sp. showed significant antibacterial activity against multidrug-resistant S. aureus strains (1-10) and P. aeruginosa strains (1-8). The minimum inhibitory concentration of the ethyl acetate extracts of culture filtrate and fruiting bodies ranged from 225 μg/mL to 625 μg/mL, and 120 μg/mL to 625 μg/mL, respectively, against clinical strains of S. aurues and P. aeruginosa. The synergistic action of extracts of Xylaria sp. with vancomycin and ciprofloxacin was observed against S. aureus strain 6 and P. aeruginosa strain 3, respectively. The fractional inhibitory concentration indices (FICIs) of culture filtrate extract with vancomycin and ciprofloxacin were 0.5 and 0.18, respectively. The FICI of fruiting body extract with vancomycin and ciprofloxacin were 0.5 and 0.375, respectively. These results clearly indicate that the metabolites of culture filtrate and fruiting bodies of Xylaria sp. are the potential source for production of new antimicrobial compounds.     

Study on the antibacterial potential of physalis minima linn

Physalis minima is an important medicinal plant of Indian System of Medicine. This plant is reported for its diuretic, laxative and antiinflammatory activities. However, the plant is not well scrutinized for its antimicrobial potential. The major chemical constituents reported from the plant are phenolics and alkaloids, which suggest that the plant may turn out to be a potent antiinfective agent. The aim of the study was to find out the antibacterial potential of mature berries of P. minima using streak plate, well diffusion, determination of minimum inhibitory concentration and bioautographic methods against a battery of Gram positive and Gram negative bacterial strains. Results of the study showed that methanol and chloroform extracts of P. minima exhibited potent inhibitory activity against all the bacterial strains tested. Minimum inhibitory concentration found out was 100 μg in both the extracts. Bioautography assay showed polar compounds present in the crude extract are responsible for the antimicrobial action.     

两种土荆皮习用药材提取物体外抗真菌活性研究

目的研究市场两种习用土荆皮代用药材的体外抗真菌活性,为土荆皮的习用药材(水翁皮与余甘子皮)的药理应用提供依据。方法采用药基琼脂稀释法,研究水翁皮与余甘子皮的提取物对11株浅表性皮肤癣菌的最小抑菌浓度(MIC)和最低杀菌浓度(MFC)。结果土荆皮的提取物对武汉须毛癣菌、武汉近平滑癣菌的MIC和MFC3.13μl/ml,对其余9株皮肤癣菌的MIC和MFC6.25μl/ml,水翁皮与余甘子皮的提取物对试验11株皮肤癣菌的MIC和MFC>50μl/ml,达克宁的MIC和MFC<0.78μl/ml。结论土荆皮的两种习用药材水翁皮与余甘子皮体外对11株皮肤癣菌未显示出抗真菌活性。     

Antibacterial activity and cytotoxicity of selected Egyptian medicinal plants

Medicinal plants have been used as a source of remedies since ancient times in Egypt. The present study was designed to investigate the antibacterial activity and the cytotoxicity of the organic extracts from 16 selected medicinal plants of Egypt. The study was also extended to the isolation of the antiproliferative compound jaeschkeanadiol p-hydroxybenzoate (FH-25) from Ferula hermonis. The microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against twelve bacterial strains belonging to four species, Providencia stuartii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, while a resazurin assay was used to assess the cytotoxicity of the extracts on the human pancreatic cancer cell line MiaPaCa-2, breast cancer cell line MCF-7, CCRF-CEM leukemia cells, and their multidrug resistant subline, CEM/ADR5000. The results of the MIC determination indicated that all the studied crude extracts were able to inhibit the growth of at least one of the tested bacterial species, the best activity being recorded with the crude extracts from F. hermonis and Vitis vinifera, whichwere active against 91.7% and 83.3% of the studied bacteria, respectively. The lowest MIC value of 128 μg/mL was recorded against P. stuartii ATCC 29916 and E. coli ATCC 10536 with the extract from V. vinifera and Commiphora molmol, respectively. In the cytotoxicity study, IC50 values below 20 μg/mL were recorded for the crude extract of F. hermonis on all four studied cancer cell lines. FH-25 also showed good cytotoxicity against MCF-7 cells (IC50: 2.47 μg/mL). Finally, the results of the present investigation provided supportive data for the possible use of the plant extracts investigated herein, mostly F. hermonis and V. vinifera in the treatment of bacterial infections and jaeschkeanadiol p-hydroxybenzoate in the control of cancer diseases.     

Indian Medicinal Plants: A Potential Source for Anticandidal Drugs

Alcoholic and aqueous crude extracts of 37 traditionally-used medicinal plants were screened for antifungal activity against the pathogenic yeast, Candida albicans, and dermatophytes, Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrum. Alcoholic extracts of these 37 plants were demonstrated to have antifungal activity. Twenty-one extracts showed strong activity and the remaining 16 plants exhibited moderate activity against Candida albicans and to one or more dermatophytes. Such activity was detected in only 16 aqueous extracts. Alcoholic extracts of five medicinal plants were selected for further studies on the basis of their strong anticandidal activity. Minimum inhibitory concentration (MIC) values of these five extracts revealed their varied anticandidal potency. MIC values ranged between 4-9 mg/ml. Comparatively high potency was observed in the extracts of Plumbago zeylanica (4mg/ml) followed by Terminalia bellirica and Phyllanthus emblica (7 mg/ml), each Holarrhena antidysentrica (8 mg/ml), and least in Terminalia chebula (9 mg/ml). Preliminary phytochemical analysis of these extracts showed the presence of many biologically active constituents.     

Antifungal activity of Boerhavia diffusa against some dermatophytic species of Microsporum

Various extracts petroleum ether, chloroform, ethyl acetate and ethyl alcohol) of aerial and root parts of Boerhavia diffusa was sereened for Antitungal activity (Inhibition in sporulation) against dermatophytic fungi Microsporum gypseum, M. fulvum and M. canis by using broth dilution method. Extracts of aerial part not show any noticeable antifungal activity. Ethyl acetate extract of root part of the plant was found to be most effective of against target fungal species. The maximum inhibition of mycelial growth was observed for M. gypseum (78.83%) followed by M. fulvum (62.33%) and M. canis (42.30%) of ethyl acetate in the test concentration of 1000 microg/ml 24 hours of incubation. The sporulation of target fungal species decreases with increasing supplementation of phytoextract, confirms the presence of some antifungal phytochemical moiety in roots of the plant.     

Antibacterial,antifungal, and antimycobacterial activity of Ilex aquifolium leaves

The objective of this study was to examine antibacterial, antifungal, and antimycobacterial properties of Ilex aquifolium L. (Aquifoliaceae) growing in Turkey. The ethanol, ethyl acetate, chloroform, and n-hexane extracts prepared from the leaves of I. aquifolium were tested against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter aerogenes, Proteus vulgaris, Salmonella typhimurium, and Candida albicans for antibacterial and antifungal evaluation using the microdilution broth susceptibility assay. In addition, antimycobacterial activity of the crude extracts of I. aquifolium was evaluated by microplate Alamar blue assay. The results showed that the extracts tested, except n-hexane, possessed moderate antibacterial and antifungal activity varying from 62.5 to 250?μg/mL. On the other hand, the ethanol extract of the leaves exhibited a minimum inhibitory concentration value (MIC) against Mycobacterium tuberculosis H₃₇Ra strain of 200 μg/mL.     

Antibacterial and Antifungal Activities of Nine Medicinal Plants from Zaire

Abstract

Zairean medicinal plants were extracted and screened for in vitro antibacterial and antifungal activities. Of nine plants investigated, extracts of six plants (Alchornea cordifolia, Bridelia ferruginea, Eucalyptus citriodora, Hymenocardia acida, Maprounea africana and Sida rhombifolia) demonstrated a marked antibacterial activity against the microorganisms Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus mutans; and extracts of four plants (A. cordifolia, E. citriodora, M. africana and S. rhombifolia) exhibited significant antifungal activity against Aspergillus niger, Candida albicans and Microsporum gypseum. Extracts from A. cordifolia and M. africana were the most active with a MIC value of 62.5 μg/ml. Fractionation of the crude methanol extracts revealed that the bactericidal and fungicidal constituents of the active plants were mainly distributed in their ethyl acetate and aqueous fractions. These results may provide a basis for the isolation of compounds of biological interest from these medicinal plants.     

Antifungal activity of the Algerian Lawsonia inermis (henna)

Context: Lawsonia inermis Linn. (Lythraceae) or henna has been used since the earliest times as a medicine, preservative, and cosmetic. It has long been recommended in traditional medicine as an astringent, purgative, and abortifacient.

Objective: Lawsone and six extracts of L. inermis plant, used by Algerian traditional healers to treat infectious diseases, were screened for their antifungal activity against filamentous fungi.

Materials and methods: Water and five organic extracts – DMSO, ethanol, chloroform, ethyl acetate, and di-ethyl ether – of L. inermis leaves, collected in the area of Adrar (Algeria), were prepared by soaking 25?g of powdered plant in 100?mL of solvent. The extracts were screened for antifungal activity using the poisoned food technique against five filamentous fungi.

Results: Results demonstrated that the best yield (8.03%) was obtained with the ethanol extract. The commercial lawsone showed potentially interesting MICs against the strains Fusarium oxysporum (12 µg/mL) and Aspergillus flavus (50 µg/mL). The ethanol extract showed the only interesting MIC (230 µg/mL of crude extract) against the strain F. oxysporum compared with other extracts.

Discussion and conclusion: These results suggest that the Algerian L. inermis plant has antifungal activity that can be related to the presence of lawsone in the leaves plant. The results can be exploited largely in research of new antifungal drugs.     

Antifungal and antioxidant activities of organic and aqueous extracts of Annona squamosa Linn. leaves

An increasing demand for natural additives has shifted the attention from synthetic to natural antioxidants and antifungal agents. This study was carried out to evaluate the antifungal and antioxidant activities of methanol, chloroform, and aqueous extracts of Annona squamosa Linn. leaves. The antifungal activities of all extracts of A. squamosa leaves against five different strains of fungi (Alternaria alternata, Candida albicans, Fusarium solani, Microsporum canis, and Aspergillus niger) were evaluated by the agar well diffusion method and the minimum inhibitory concentration of each extract was assessed by antifungal susceptibility using the broth microdilution method. The antioxidant potential of each extract was determined by free radicals (1,1-diphenyl-2-picrylhydrazyl, nitric oxide, and hydrogen peroxide) scavenging activity and reducing power property of A. squamosa leaves. Both organic and aqueous extracts were found to express dose-dependent inhibition against all tested fungi strains in both agar well diffusion and broth dilution methods. The free radical scavenging activity and reducing power property of all extracts were found to be concentration dependent, with the methanol extract exhibiting higher antioxidant activity than the chloroform extract, which was more effective than the aqueous extract of A. squamosa leaves. Results of phytochemical analysis of extracts showed the presence of glycosides, saponins, tannins, flavonoids, phenols, etc. The results obtained from in vitro studies of antifungal and antioxidant activities clearly suggest that the methanol, chloroform, and aqueous extracts of A. squamosa leaves possess antifungal and antioxidant activity.     

Marine algae: screening for a potent antibacterial agent

This study was done to investigate the antimicrobial potentiality of the marine algae collected from different coastal regions of Gujarat and screened for the same. Twenty-six marine algae belonging to Rhodophyceae, Chlorophyceae and Phaeophyceae were screened for their potential antibacterial activity against five clinically important bacterial strains, namely Bacillus cereus, Micrococcus flavus, Citrobacter freundii, Klebsiella pneumoniae and Pseudomonas testosterone. Acetone and methanol were used for extraction; and the extracted yield was more when the solvent used was methanol. The antibacterial activity was done by both Agar disc diffusion method and Agar ditch method. The five bacterial strains showed varied response towards marine algal extracts. The most susceptible bacteria was B. cereus followed by K. pneumoniae and C. freundii while the most resistant bacteria were M. flavus and P. testosteroni. Among the 26 algae screened, E. intestinalis was the most potent alga and thus, this alga was selected for further studies. E. intestinalis was extracted in petroleum ether, 1,4-dioxan, acetone, methanol and DMF, and their antibacterial activity was studied against the above-stated five bacterial strains using agar disc method. Maximum extractive value of E. intestinalis was in methanol (2.05%) and minimum was in acetone (0.38%). The most susceptible bacteria was K. pneumoniae and maximum antibacterial activity was shown by petroleum ether extract and minimum was shown by 1,4-dioxan extract. The most resistant bacteria were M. flavus and C. freundii. The MIC values of E. intestinalis extracts ranged from 2500-9.765 microg/0.5 ml against B. cereus and K. pneumoniae. From these results it is concluded that the acetone extract of E. intestinalis is the most potent extract and can be used as a lead molecule in drug discovery in inhibiting some of the bacterial strains. E. intestinalis can be used as a promising novel marine antimicrobial agent in the coming years.     

Antibacterial and Antifungal Potential of some Arid Zone Plants

Sequential extracts of some medicinally important arid zone plants of Rajasthan, viz. Lepidagathis trinervis Nees., Polycarpea corymbosa Lam. and Sericostoma pauciflorum Stocks. ex Wight. were tested against six bacterial (Gram +ve and Gram -ve) and five fungal strains using agar well diffusion method. Ethyl acetate extract of L. trinervis showed maximum activity against Bacillus subtilis, Enterobactor aerogenes, Pseudomonas aeruginosa, Aspergillus flavus and Trichophyton rubrum (inhibition zone 16.00±0.81, 13.33±0.66, 14.33±1.85, 14.30±0.34 and 23.00±0.00 mm) at varied minimum inhibitory concentrations of 82, 20, 41, 41 and 20 μg/ml, respectively.     

Phytochemistry and Antibacterial Activity of Senna alata Flower

Phytochemistry and antibacterial potency of some crude and partially purified fractions of Senna alata flower was examined against 22 bacterial strains. The crude plant extracts, containing steroids, anthraquinone glycosides, volatile oils and tannins, exhibited a high minimum inhibitory concentration of 500 µg/mL against Staphylococcus aureus, Streptococcus faecalis, Micrococcus luteus, Bacillus subtilis and Pseudomonas putida, but was generally inactive against Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, and Pseudomonas fluorescens (above 1000 µg/mL). However, the partially purified plant extract was bacteriostatic at a low concentration of 100 µg/mL, with a minimum bactericidal concentration of 500 µg/mL, primarily against the Gram positive organisms. At a concentration slightly above the minimum inhibitory concentration, the purified extract was nearly as potent as standard antibiotics, exhibiting zones of bacterial growth inhibition ranging from 10 to 25 mm, even against multiple antibiotic resistant local isolates that were not susceptible to methicillin, penicillin and streptomycin.     

Antibacterial and antifungal activities of extracts of combretum molle

OBJECTIVE: In traditional medical practices of Ethiopia the aqueous extracts obtained from the stem bark of Combretum molle (R. Br. Ex. G. Don.) Engl & Diels (Combretaceae) have a longstanding reputation for the treatment of liver diseases malaria and tuberculosis. Owing to the widespread traditional uses of this plant, the studyinvestigated the antimicrobial activity the bark extract of this plant against Gram positive and and Gram negative bacteria. METHODS: Petroleum ether, dichloromethane and acetone fractions of the bark of the plant were prepared by soxhlet extraction and screened for their antimicrobial activity. The acetone fraction exhibited a powerful activity and was therefore further tested against twenty-one bacterial and six fungal strains. The minimum inhibitory concentration (MIC) of this extract was determined by checker board technique using nutrient agar medium. The zones of inhibition produced by the extract against bacteria and fungi were determined and compared by disc diffusion technique with those of pure ciprofloxacin and griseofulvin, respectively. RESULTS: The highest antibacterial action of the acetone extract was against the Gram negative organisms EscherIchia coli and Shigella spp with an MIC value of 50 mg/ml. The activity of the extract against these bacteria was comparable to that of ciprofloxacin when assessed by the disc diffusion technique. Among the fungal strains tested Candida albicans showed high susceptibility to the extract and growth was completely inhibited at a concentration of 400 microg/ml. At the same concentration, the acetone extract and the standard antifungal drug griseofulvin produced comparable zones of inhibition on C. albicans. Studies on the mode of action of the extract indicated that it was bactericidal and fungicidal. The antimicrobial activity of the extract was attributed to the high amount of hydrolysable tannins present in the bark of the plant. CONCLUSION: The acetone extract of the stem bark of C. molle has the potential for use as a natural antimicrobial agent. Further in vivo antimicrobial, phytochemical and toxicological studies are requireed to evaluate the chemotherapeutic effect of the plant.     

The anti-dermatophyte activity of Commiphora molmol

Context: Commiphora molmol Engl (Burseraceae) or myrrh has been traditionally used for the treatment of skin fungal infections.

Objective: This study evaluates the antifungal activity of myrrh ethanol extract and essential oil against skin dermatophytes.

Materials and methods: The antifungal evaluations were performed by the food poisoning technique (250?ppm) and micro-broth dilution assay (800–6.25?µg/mL) against Trichophyton rubrum, T. mentagrophytes, Microsporum canis, M. gypseum, and T. verrucosum. The chemical composition of myrrh oil and ethanol extract was analyzed by GC and GC-MS.

Results: Furanoeudesma 1,3-diene and menthofuran were the main components of myrrh oil, while 2-tert-butyl-1,4-naphthoquinone, benzenemethanol,3-methoxy-α-phenyl, and curzerene were the main components of myrrh ethanol extract. The inhibitory effect of myrrh oil and ethanol extract against dermatophytes were 43.1–61.6% and 12.5–27.5%, respectively. The MIC and MFC values of myrrh oil were 25–100 and 25–200?µg/mL while these amounts for ethanol extract were 25–400 and 25–400?µg/mL, respectively. Therefore, myrrh oil had higher antifungal activity than that of the ethanol extract. Both extracts showed good anti-elastase activity.

Conclusion: The results of our investigation confirmed the traditional uses of C. molmol as a poultice for the treatment of cutaneous fungal infections.     

Antimicrobial activity and phytochemical screening of serial extracts from leaves of Aegle marmelos (Linn.)

The in vitro antimicrobial activity of serial petroleum ether, chloroform and methanol extracts from leaves of Aegle mawmelos were investigated against bacterial and fungal species. All the extracts exhibited broad spectrum antimicrobial activity with zones of inhibition ranging from 10 to 22 mm against bacteria: Staphylococcus aureus, beta Streptococcus haemolyticus group A, Proteus mimrabilis, Klebsiella pneumoniae, Pseudomonas aenrginosa, Escherichia coli, Salmonella typhi, fungi: Candida albicans, Candida tropicalis and Aspergillusflavus. The minimal inhibitory concentrations (MIC) and the minimal microbicidal concentrations (MMC) of the extracts ranged from 1.25 to 10 mg/mL and 2.5 to 20 mg/mL respectively. Assessment of antibacterial efficacy of different extract revealed that Staphylococcus aureus, beta Streptococcus haemolyticus group A, Pseudomonas aeruginosa and Escherichia coli showed high susceptibility to petroleum ether extract. Proteus mimrabilis, Klebsiella pneumoniae showed high susceptibility to chloroform extract and Salmonella typhi showed high susceptibility to methanol extract. Petroleum ether extract exhibited the highest antifungal efficacy against all tested fungal species. Phytochemical screening revealed the presence of phenols, sterols in petroleum ether and chloroform extracts, whereas tannins, flavonoids, coumarins, saponins and triterpenoids in methanol extract. The ability of the leaf extracts of Aegle manmelos to inhibit growth of bacteria and fungi is an indication of its broad spectrum antimicrobial activity which could be a potential source for development of novel bioactive antimicrobial agents.     

Potent antifungal activity of garlic (allium sativum) against experimental murine dissemenated cryptococcosis

The adequate management of cryptococcosis is difficult with the available armamentarium of synthetic antifungals. We made an effort to evaluate fresh extracts of garlic ( Allium sativum L.) to assess potential therapeutic use of this herb as an anticryptococcal agent. The in vitro antifungal activity in broth showed greatest activity in the aqueous extract (MIC 125-250 μg/ml; MFC 250 to = 500 μg/ml) compared to the alcoholic, crude or commercial extracts. Treatment of Balb/c mice with garlic extracts in mice (orally) showed that alcoholic extracts (5 mg/kg, q.i.d.) provided 100% protection of mice against disseminated cryptococcosis. A concomitant increase in MST (> 35 days) was observed, along with a significant reduction in cfu (log 10 ) burden of cryptococci in visceral (liver, spleen, kidney, lung and heart) tissues, especially brain (> 4.5 log 10 ). The efficacy of extracts was also evident in the sera of mice. Thus, alcoholic extracts of fresh garlic elicit potential anticryptococcal activity against murine disseminated cryptococcosis.     

Design and synthesis of novel piperazine unit condensed 2,6-diarylpiperidin-4-one derivatives as antituberculosis and antimicrobial agents

Abstract  

A new series of 1-[2-(4-ethoxycarbonylpiperazine-1-yl)acetyl]-2,6-diarylpiperidin-4-ones (3a–3j) has been synthesized by conventional method and were characterized by IR, elemental analysis, mass spectral, ¹H NMR, ¹³C NMR, and single crystal X-ray diffraction analysis. The synthesized compounds were evaluated for their antituberculosis activity against Mycobacterium tuberculosis H37Rv (ATCC-27294) and also its antimicrobial activity were examined against five familiar bacterial and fungal strains. Among the synthesized compounds, compounds 3e–3j exhibit higher inhibition potency (16 μg/ml) against M. tuberculosis H37Rv. Furthermore, compounds containing fluoro substituent in the phenyl ring at C-2 and C-6 positions of the piperidin-4-one motif (compounds 3c, 3d, and 3i) exerted better antibacterial and antifungal activity than the other phenyl-substituted compounds.