多动行为问题儿童血5-羟色胺浓度变化相关性探讨

目的探讨多动行为问题儿童与正常儿童外周血5-羟色胺浓度差别;进一步了解心理卫生偏异儿童、不同年龄阶段行为问题儿童外周血5-羟色胺浓度差别,并分析各种行为问题儿童外周血5-羟色胺浓度的变化及其意义。方法应用《Achenbach儿童行为量表》筛查并根据其因子分分类,对30例行为问题儿童进行外周血5-羟色胺含量测定,与30例正常儿童对照比较。结果多动行为问题儿童与正常儿童外周血5-羟色胺浓度比较明显降低,差异显著(P<0.05);内向型因子组与外向型因子组比较明显升高,差异显著(P<0.05);不同年龄阶段行为问题儿童外周血5-羟色胺浓度随着年龄的增长与正常儿童差异增大。结论外周血5-羟色胺浓度与行为问题有关,可能是儿童行为问题发生的物质基础之一。     

多动行为问题儿童血5—羟色胺浓度变化相关性探讨

目的：探讨多动行为问题儿童与正常儿童外周血5－羟色胺浓度差别，进一步了解心理卫生偏异儿童，不同年龄阶段行为问题儿童外周血5－羟色胺浓度差别，并分析各种行为问题儿童外周血5－羟色胺浓度的变化及其意义。方法：应用《Achenbach儿童行为量表》筛查并根据其因子分分类，对30例行为问题儿童进行外周血5－羟色胺含量测定，与30例正常儿童对照比较。结果：多动行为问题儿童与正常儿童外周血5－羟色胺浓度比较明显降低，差异显（P＜0．05），内向型因子组与外向型因子组比较明显升高，差异显（P＜0．05），不同年龄阶段行为问题儿童外周血5－羟色胺浓度随着年龄的增长与正常儿童差异增大，结论：外周血5－羟色胺浓度与行为问题有关，可能是儿童行为问题发生的物质基础之一。     

不同年龄阶段行为偏异儿童血5-羟色胺检测及其意义

为探讨不同年龄阶段行为偏异儿童血5-羟色胺(5-HT)含量与正常儿童血5-HT含量的差异,寻找其变化规律,为早期干预提供理论依据。应用《Achenbach儿童行为量表》筛查出行为偏异儿童46人,对其中30人检测血5-HT浓度;正常对照组30人。结果,行为偏异组儿童5-HT含量(1.3093±0.8174μmol/L)较正常儿童组(1.1112±0.5673μmol/L)高,但没有显著性差异(t=1.0860、P>0.05),行为偏异儿童不同年龄阶段比较:5-HT含量13～16岁组、6～11岁与4～5岁组比较有显著性差异(t=3.2274,P分别<0.0025、0.001),12～16岁组较6～11岁组高,但没有显著性差异(t=0.6274,P>O.05);与同年龄正常组比较;5-HT含量4～5岁、6～11岁组无显著性差异(t=0.0868、1.0065,P均>0.05),12～16岁行为偏异组5-HT含量显著增高(t=1.7264,P<0.05)。提示:不同年龄阶段行为偏异儿童外周血5-HT含量改变与不同年龄阶段有关,随着年龄增长5-HT浓度渐增高,且与正常儿童差异增大。早期干预效果可能会更好。     

氦氖激光治疗偏头痛与血小板5-HT的关系

本文报道用ＨｅＮｅ激光治疗偏头痛３３例全部有效，其中典型偏头痛１８例，痊愈５５６％（１０／１８），显效３３３％（６／１８），有效１１１％（２／１８）；普通型偏头痛１５例中，痊愈５３３３％（８／１５），显效２００％（３／１５），有效２６６７％（４／１５）。观察了激光治疗前后患者血小板５—ＨＴ浓度变化，结果发现：３３例偏头痛和其中８例痊愈普通型偏头痛患者在治疗前后血小板５—ＨＴ浓度均有显著性差异（ｐ＜００５）；８例痊愈典型偏头痛患者在治疗前后血小板５—ＨＴ浓度无显著性差异（ｐ＞００５）。我们认为：从整体均值来看，偏头痛患者血小板５—ＨＴ浓度低下是其发病原因，而激光治疗则可增加血小板中５—ＨＴ的含量。     

儿童多动症、儿童自闭症与血中单胺类神经递质关系的研究

目的了解儿童多动症即注意缺陷多动障碍(attentiondeficitdisorderwithhypractivity,ADHD)与儿童自闭症(autistcdisorder,AD)的行为与血中单胺类神经递质去甲肾上腺素(NE),5-羟色胺(5-HT)的关系。方法采用德国进口的检测血中去甲肾上腺素,5-羟色胺试剂(IMMUNOBIOLOGICALLABORATORIES,IBL)Noradrenalin(e)ELISA及SerotoninELISA,使用ThermoLabsystems雷勃酶标仪(MK3)测定。结果与正常儿对比,儿童多动症血中NE显著升高,5-HT显著下降,儿童自闭症血中5-HT显著升高,NE显著下降。结论血中单胺类神经递质与儿童的行为关系密切,检测血中NE、5-HT可以了解儿童行为模式起一定的作用。     

5，7-DHT诱导5-HT神经元毁损致小鼠抑郁表现

目的：通过侧脑室注射5-HT（5-羟色胺，5-Hydroxytryptamine）类似物5,7-DHT（5，7-双羟色胺．5,7-dihydroxytryptamine），毁损中缝背核的5-HT能神经元，通过行为学评估研究其是否导致小鼠抑郁样症状，探讨5-HT在抑郁发病机制中的作用。方法：C57BL/6J小鼠经脑室注射5，7-DHT后14天，观察体重变化，并使用强制游泳实验、悬尾实验评估其是否出现抑郁症状，并用免疫组织化学方法观察中缝背核5-HT神经元的残存情况。结果：侧脑室中注射5，7-DHT后14天，脑中中缝背核的5-HT能神经元几乎完全消失，被GFAP阳性的星形胶质细胞所代替，小鼠体重几乎不增加、强制游泳实验和悬尾实验中的“无动时间”均明显延长，表现出明显的抑郁症状。结论：5，7-DHT可以毁损5-HT神经元，引起抑郁症状，提示5-HT在抑郁症的发病机制中发挥着-定作用。a     

帕罗西汀治疗原发性早泄后血浆5-羟色胺浓度的变化

目的了解帕罗西汀治疗原发性早泄后血浆5-HT浓度的变化以及此种变化和临床症状改善程度之间的可能关系。方法筛选本院门诊原发性早泄患者81例,根据患者近3次性生活阴道内射精潜伏期(intra-vaginal ejaculation latency time,IELT)的平均值将入选样本分为两组:A组(IELT≤30s),B组(30sIELT≤60s)。同时对每一个患者予以提取血浆冻存。两组患者均口服帕罗西汀20mg/d,连用8周。治疗8周后再次予以同样的方法记录IELT,提取患者血浆。采用5-HT ELISA试剂盒测定所收集的血浆样本。运用SPSS16.0软件统计分析所得数据。结果研究初始,研究终点时,共计125例患者获得了可靠随访数据及血样信息,其中A组41例,B组40例。研究发现,研究初始时,A组患者血浆5-HT浓度显著低于B组(P0.001),治疗后,血浆5-HT浓度及IELT均显著增加(P0.001),A组IELT均值的增加以及5-HT浓度的增加均显著高于B组,(P0.001)。结论原发性早泄患者血浆5-HT浓度越低的患者表现出了越严重的早泄症状。原发性早泄患者在通过帕罗西汀治疗后5-HT浓度显著增高且治疗前5-HT浓度越低的患者治疗后其5-HT浓度增高越明显,且其临床症状改善越明显,5-HT浓度的提高与患者早泄症状的改善之间具有明显的一致性。     

儿童多动症、儿童自闭症与血中单胺类神经递质关系的研究

目的：了解儿童多动症即注意缺陷多动障碍（attention deficit disorder with hypractivity,ADHD)与儿童自闭症（autistc disorder,AD)的行为与血中单胺类神经递质去甲肾上腺素（NE，5－羟色胺（5－HT）的关系。方法：采用德国进口的检测血中去甲肾上腺素，5－羟色胺试剂（IMMUNOBIOLOGICAL LABORATORIES，IBL）Noradrenalin(e) ELISA及Serotonin ELISA,使用Thermo Labsystems雷勃酶标仪（MK3）测定。结果：与正常儿对比，儿童多动症血中NE显著升高，5－HT显著下降，儿童自闭症血中5－HT显著升高，NE显著下降。结论：血中单胺类神经递质与儿童的行为关系密切，检测血中NE、5－HT可以了解儿童行为模式起一定的作用。     

儿童行为问题与家庭动力学特征关系研究

目的探讨儿童行为问题与家庭动力学特征，为家庭干预提供依据。方法采用Achen—bach儿童行为父母问卷、家庭动力学自评量表和自制一般情况调查问卷对昆明市盘龙区城市、农村、城郊结合部三所小学的627名四、五年级学生进行调查分析。结果627名学生中检出行为问题者131名，阳性率20．1％，其中男生67名（21．7％），女生64名（20．1％）。相关影响因素分析中发现，单亲家庭阳性率显著高于双亲家庭（P〈O．05），双亲家庭阳性率显著低于多代同堂家庭（P〈0．05），城市家庭阳性率显著低于外来务工家庭（P〈0．05）。单因素方差分析显示，在有、无行为问题儿童家庭中，动力学个性化因子差异有极显著性（F=10．58，P〈0．01）。结论单亲家庭和多代同堂家庭儿童行为异常发生率显著高于双亲家庭；在家庭动力学因子中以个性化因子的影响较大。     

LDH患者PGE2、5-HT及炎症细胞介质的变化与患者疼痛的关系

目的探讨腰椎间盘突出症(LDH)患者血清前列腺素E2(PGE2)、5-羟色胺(5-HT)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的变化及其意义。方法选取我院2016年3月～2017年10月收治的100例LDH患者(LDH组)、选取100例健康体检对象作为对照组,检测两组的血清PGE2、5-HT、IL-1β、IL-6、TNF-α水平,并按照病理学分型将LDH组分为突起型(突起组)、破裂型(破裂组)、游离型(游离组)进行分层分析,探讨LDH患者上述指标与患者疼痛程度的关系。结果 LDH组患者的血清PGE2、5-HT、IL-1β、IL-6、TNF-α水平均显著的高于对照组,差异具有统计学意义(P0.05);游离组LDH患者的血清PGE2、5-HT、IL-1β、IL-6、TNF-α水平均显著的高于突起组、破裂组,差异具有统计学意义(P0.05);破裂组LDH患者的血清PGE2、5-HT、IL-1β、IL-6、TNF-α水平均显著的高于突起组,差异具有统计学意义(P0.05);LDH患者的血清PGE2、5-HT、IL-1β、IL-6、TNF-α水平与患者手术前的VAS疼痛程度评分均呈显著的正相关关系(P0.05)。结论 LDH患者PGE2、5-HT及炎症细胞介质水平较健康人群显著的升高,并且与LDH患者疼痛程度及病理分型有关。     

Ⅰ型和Ⅱ型精神分裂症血清5-羟色胺功能的初步研究

本文采用荧光光度法测定30例Ⅰ型精神分裂症和13例Ⅱ型精神分裂症患者以及26例正常人血清5-羟色胺浓度,比较了他们间的差异。初步研究结果表明,血清5-羟色胺浓度Ⅰ型病人显著低于Ⅱ型病人和对照组(P<0.01),而后两者间无明显变化(t=1.20,P>0.05)。作者从5-羟色胺和多巴胺代谢中的生化联系对Ⅰ型和Ⅱ型精神分裂症的病理生理机制作了初步讨论。     

血清胆红素水平与非杓型高血压的关系研究

【目的】探讨血清胆红素水平与非杓型高血压之间的关系。【方法】选择本院2012年3月至2013年12月收治的80例高血压患者,经24 h动态血压监测,分为杓型组（ n ＝50,杓型高血压患者）和非杓型组（ n＝30,非杓型高血压患者）,检测比较两组患者的24 h动态血压、血清胆红素（BIL ）、空腹血糖（FPG ）、肌酐（SCr）、总胆固醇（TC）、电解质（Na＋、K＋、Ca2＋）、谷草转氨酶（AST）、谷丙转氨酶（ALT）、碱性磷酸酶（ALP）、促甲状腺激素（TSH）水平。【结果】夜间杓型组患者收缩压（SBP）、舒张压（DBP）均低于非杓型组,具有统计学意义（ P ＜0．01）;非杓型组患者总胆红素（TBIL）、直接胆红素（DBIL）、间接胆红素（IBIL）水平显著低于杓型组患者（P ＜0．01）;白细胞计数非杓型组显著高于杓型组（P ＜0．01）。【结论】高血压患者中较低血清胆红素水平与非杓型高血压有潜在联系。     

Mechanical, thermal and formalin-induced nociception is differentially altered in 5-HT1A-/-, 5-HT1B-/-, 5-HT2A-/-, 5-HT3A-/- and 5-HTT-/- knock-out male mice

Extensive studies in rodents suggest that serotonin (5-HT) modulates nociceptive responses through the stimulation of several receptor types. However, it remains to demonstrate that these receptors participate in the control of nociception under physiological conditions. Pain behaviors of mutants which do not express 5-HT_1A, 5-HT_1B, 5-HT_2A or 5-HT_3A receptors, or lacking the 5-HT transporter, compared to paired wild-type mice of the same genetic background, were examined using validated tests based on different sensory modalities. Mechanical (von Frey filaments, tail pressure, tail clip tests), thermal (radiant heat, 46 °C water bath, hot-plate test) and formalin-induced nociception were determined in 2- to 3-month-old males. 5-HT_1A knock-out mice differed from wild-types by higher thermal sensitivity (hot-plate test only), and 5-HT_1B knock-out mice by higher thermal and formalin sensitivity. Both 5-HT_2A and 5-HT_3A knock-out mice differed from wild-types by a dramatic decrease in the formalin-induced nociceptive responses for phase II (16–45 min after injection/inflammatory phase). In contrast, neither mechanical, thermal nor formalin-induced nociception differed between mutants lacking the 5-HT transporter and paired wild-type mice. Although differences in spontaneous locomotor activity in 5-HT_1B−/− (increase) and 5-HT_3A−/− (decrease) knock-out mice versus paired wild-types might have confounded differences in nociception, acute 5-HT receptor blockade by selective antagonists was found to replicate in wild-type mice the effects on pain behavior, but not on locomotor activity, of the respective gene knock-out in mutants. These results support the conclusion that the complex control of pain mechanisms by 5-HT, acting at multiple receptors, is physiologically relevant in mice.     

Pharmacological analysis of the cardiac effects of 5-HT and some 5-HT receptor agonists in the pithed rat

Summary— A pharmacological analysis of the effects of 5-HT on heart rate has been performed in the pithed rat. 5-HT induced a dose-dependent increase in heart rate whereas 5-HT, receptor agonists — 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), 5-methoxy-N,N-dimethyl-tryptamine (5-MeODMT), 5-methoxy 3-(1,2,3,6-tetrahydro-4-piridinyl) 1H indole (RU 24969) and 1-(m-trifluoromethylphenyl)-piperazine (TFMPP) — failed to increase heart rate. The increase in heart rate induced by the selective 5-HT₂ receptor agonist 1-(2,5-dimethoxy-4-iodo-phenyl)-2-aminopropane (DOI) was not significant. The dose-response curve to 5-HT for its tachycardic effects was shifted two-fold to the right by ketanserin and LY 53857 and nine-fold to the right by methiothepin. The effects of high doses of 5-HT (higher than 100 μg/kg iv) were antagonized by methiothepin, (-)propranolol, 2-{2-[4(O-methoxyphenyl)-piperazine-1-yl]-ethyl}4,4-dimethyl-1,3 (2H-4H) isoquinoline-dione (AR-C 239) and by pretreatment with reserpine. The 5-HT₁ receptor antagonists, pindolol and spiroxatrine, the 5-HT₃ receptor antagonist MDL 72222 and the α₂-adrenoceptor blocking agent idazoxan failed to antagonize the tachycardia induced by 5-HT. It is concluded that in the pithed rat, the tachycardia induced by 5-HT remained unexplained (implication of 5-HT₂ receptors probably different from the classical vascular 5-HT₂ receptor, or implication of 5-HT_1C receptors?). Moreover, at high doses (higher than 100 μ/kg iv), 5-HT may increase heart rate by releasing catecholamines.     

冰冻保存对机采血小板释放5-HT的影响

目的 观察冰冻保存对机采血小板释放5-HT 的影响.方法 常规以二甲基亚砜(DMSO)为冷冻保护剂、-80 ℃冰冻保存机采血小板30 d;采用全自动血细胞分析仪检测并比较冰冻保存前后血小板计数(PLT)、血小板平均体积(MPV)和血小板体积分布宽度(PDW);ELISA法检测冰冻前后及在阳离子没食子酸丙酯(C-PG)、凝血酶(thrombin,THB)、二磷酸腺苷(ADP)、胶原(Collagen)等不同PLT诱导剂激活作用下PLT释放5-HT的数量.结果 冰冻复苏后机采血小板计数变化不大(P>0.05),但MPV和PDW 均增加,血小板制品血浆5-HT含量也显著增高(P<0.01).在不同诱导剂作用下,新鲜血小板释放5-HT均高于冰冻保存的血小板,差异有显著性(P<0.01).结论 (1)机采血小板冰冻保存与解冻过程中,会引起血小板膜形态和生物活性改变.(2)冰冻保存后PLT对不同诱导剂的反应下降.     

Ⅰ型糖尿病儿童19例行为问题分析

目的：探讨Ⅰ型糖尿病患儿与正常儿童心理发育的差异。方法：应用国内最新引进的Ａｃｈｅｎｂａｃｈ儿童行为量表,（ＣＢＣＬ）对１９例Ⅰ糖尿病患儿进行调查,与河南省ＣＢＣＬ标准化分值（简称常模分）比较、分析。结果：１９例Ⅰ型糖尿病儿童行为问题因子大多在大于６９百分位数、小于９８百分位数。个别儿童抑郁分值高于９８百分位数。而交往不良、焦虑则稍低于６９百分位数。结论：对Ⅰ型糖尿病患儿心理发育应引起注意。     

按经选穴针刺对失眠大鼠下丘脑5-HT 1a、5-HT 2a受体mRNA表达的影响

目的:观察按经选穴针刺对失眠大鼠下丘脑5-HT 1a、5-HT 2a受体mRNA表达的影响,探讨针刺治疗失眠症的疗效机制及按经选穴针刺对腧穴配伍效应的影响。方法:将60只SD大鼠随机分为5组,即:空白组、模型组、百会+神门穴组、百会+三阴交穴组、百会+非经非穴组,每组12只,通过腹腔注射DL-4-氯苯基丙氨酸混悬液建立失眠模型大鼠,各治疗组针刺相应腧穴,每次30min,治疗7d。运用实时荧光定量方法检测大鼠下丘脑5-HT1a、5-HT 2a受体mRNA表达量。结果:与模型组比较,各针刺组大鼠下丘脑5-HT 1a受体mRNA表达量均有一定程度的升高,5-HT 2a受体mRNA表达量均有一定程度的降低,且以百会+神门穴组调节效果最明显,百会+三阴交穴组及百会+非经非穴组次之。结论:针刺治疗可能通过调节失眠大鼠下丘脑5-HT 1a、5-HT 2a受体mRNA的表达发挥治疗作用,且按经选穴针刺可能是影响腧穴配伍效应的影响因素之一。     

Serotonin (5-HT) and Migraine

SYNOPSIS
Serotonin, or 5-hydroxytryptamine [5-HT], is a biogenic amine implicated in controlling feeding behavior, thermoregulation, sexual behavior, and sleep. 5-HT receptors recognize at least three types of molecular structures: G protein coupled receptors, ligand gated ion channels, and transporters. It is now believed that there are at least seven different families of receptors, many of which have subtypes. The nervous system can be compared to a group of well-modulated neural networks functioning in parallel. The serotonergic system may modulate these networks rather than actually mediate individual responses. Circumstantial evidence suggests a link between 5-HT and migraine. Platelet HT decreases during an attack, and in some cases increased levels of metabolites are found. Many antimigraine drugs interact with 5-HT and its receptors.     

Role of peripheral 5-HT(4), 5-HT(6), and 5-HT(7) receptors in development and maintenance of secondary mechanical allodynia and hyperalgesia

The role of 5-hydroxytryptamine (5-HT)₄, 5-HT₆, and 5-HT₇ receptors in formalin-induced secondary allodynia and hyperalgesia in rats was assessed. Formalin produced acute nociceptive behaviors (flinching and licking/lifting) followed by long-term secondary mechanical allodynia and hyperalgesia. Pretreatment (−10 min) with cromoglycate (195-1950 nmol/paw) partially inhibited acute nociceptive behaviors and completely prevented secondary allodynia and hyperalgesia on day 6 after injection. Ipsilateral peripheral pretreatment with the selective 5-HT₄ (ML-10302, 1-100 nmol/paw), 5-HT₆ (EMD-386088, 0.001-0.01 nmol/paw), and 5-HT₇ (LP-12, 0.01-100 nmol/paw) receptor agonists significantly increased secondary allodynia and hyperalgesia in both paws. In contrast, ipsilateral peripheral pretreatment with the selective 5-HT₄ (GR-125487, 1-100 nmol/paw), 5-HT₆ (SB-258585, 0.00001-0.001 nmol/paw), and 5-HT₇ (SB-269970, 0.1-10 nmol/paw) receptor antagonists significantly prevented formalin-induced secondary allodynia and hyperalgesia in both paws. The pronociceptive effect of ML-10302 (100 nmol/paw), EMD-386088 (0.01 nmol/paw), and LP-12 (100 nmol/paw) were completely prevented by GR-125487 (5-HT₄ antagonist, 1 nmol/paw), SB-258585 (5-HT₆ antagonist, 0.00001 nmol/paw), and SB-269970 (5-HT₇, antagonist, 0.01 nmol/paw), respectively. Ipsilateral peripheral posttreatment with cromoglycate or GR-125487 (1-100 nmol/paw), SB-258585 (0.001-0.1 nmol/paw), and SB-269970 (0.1-10 nmol/paw) reversed formalin-induced secondary allodynia and hyperalgesia in both paws. Results suggest that a barrage of afferent input induced by 5-HT at peripheral 5-HT₄, 5-HT₆, and 5-HT₇ receptors participate in the development and maintenance of formalin-induced long-term secondary allodynia and hyperalgesia in the rat.