磁共振成像测量的浸润深度与舌鳞状细胞癌患者颈部淋巴结转移的相关性研究

目的 分析磁共振成像(MRI)测量的浸润深度(DOI)与临床颈部淋巴结阴性即cN0期舌鳞状细胞癌患者颈部淋巴结转移的相关性,明确MRI测量的DOI是否可作为cN0期舌鳞状细胞癌患者颈部淋巴结转移的独立预测因素.方法 经纳入和排除标准筛选后选取61名舌鳞状细胞癌的患者,分析纳入患者中MRI测量的肿瘤DOI及其他临床病理因...     

MRI测量和评估口腔黏膜鳞状细胞癌临床分期指标的准确性分析

目的 ：研究磁共振成像（MRI）测量和评估口腔黏膜鳞状细胞癌（oral squamous cell carcinoma,OSCC）临床分期指标的准确性。方法：纳入2020年2月—2022年9月就诊于中国科学技术大学附属第一医院的OSCC患者71例,记录MRI测量的肿瘤最大直径（maximum tumor diameter,MTD）、浸润深度（depth of invasive,DOI）以及最大值截面,按相应截面制作病理切片,测量病理MTD和DOI值并进行统计分析,将MRI图像评估的患者颈淋巴结状态与术后病理证实的淋巴结状态进行比较,分析术前MRI测量和评估临床分期的准确性。采用SPSS 26.0软件包对数据进行统计学分析。结果：MRI Gd-T1WI序列测量的DOI平均较病理结果高出2.54 mm(95%CI:3.56～1.53,P<0.05),相关系数r为0.984;T2WI序列测量的DOI平均较病理结果高出3.09 mm (95%CI:4.88～1.29,P<0.05),相关性系数r为0.953。Bland-Altman散点图显示,Gd-T1WI序列的测量结果与病理DO...     

舌鳞状细胞癌浸润前沿细胞增殖的研究

目的　研究舌磷癌浸润前沿分级、检测增殖细胞核抗原 (PCNA)、Ki6 7、核仁组织区相关嗜银蛋白 (AgNOR)在浸润前沿中的表达。 方法　对 5 7例舌鳞癌行浸润前沿分级 ,采用免疫组化SP法及银染色法检测浸润前沿和中心PCNA、Ki6 7、NORs的表达。结果　浸润前沿PCNA、Ki6 7的表达和AgNOR均数均明显高于非前沿部分 ,差异有极显著性 (P <0 .0 0 1) ;浸润前沿PCNA标记指数 (P <0 .0 5 )、AgNOR均数 (P <0 .0 1)与浸润前沿分级 (IFG)总分呈正相关关系 ;单因素COX分析显示 ,IFG总分 (P <0 .0 1)、浸润前沿AgNOR均数 (P <0 .0 5 )对判断预后有意义 ;多因素COX分析显示 ,IFG总分 (P <0 .0 1)可作为预测总体生存率和累积生存率的有意义的因子。结论　舌鳞癌浸润前沿肿瘤细胞分化较差 ,增殖活性明显高于非前沿部分 ;IFG总分和浸润前沿AgNOR均数与舌癌预后有关     

3．0TMRI在舌鳞状细胞癌术前诊断和临床评估中的应用价值

目的：探讨3．0 T高场强MRI成像技术在舌鳞状细胞癌术前诊断和临床评估中的应用价值。方法：回顾性分析本院经手术和病理证实为舌鳞癌的26例病例术前3．0 T MRI影像资料。结果：3．0 T MRI可以清晰显示舌鳞癌及其侵犯的范围,同时了解颌下及颈部淋巴结情况,对TN分期具有重要意义。结论：3．0 T MRI对舌鳞癌的显示和诊断具有高分辨率、多方位及多序列成像优势,可以很好地显示舌鳞癌发生的位置、大小、病变侵及范围以及扩散途径,有助于术前病变性质的判断及肿瘤分期的评估。舌鳞癌检查首选高场强MRI。     

早期口腔鳞状细胞癌浸润深度检查方法的研究进展

口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)是口腔颌面部的常见恶性肿瘤,美国癌症联合委员会第8版口腔癌分期中,纳入了浸润深度(depth of invasion, DOI)进一步细化T分期,术前精准的浸润深度对于颈淋巴清扫术的实施与否提供治疗决策,同时也有益于预后的评估。本文对近年来组织病理学检查、超声检查、电子计算机断层扫描(CT)、核磁共振成像(MRI)检查测量OSCC浸润深度的相关研究作一综述。     

舌鳞状细胞癌预后因素的探讨

目的：探讨影响舌鳞癌预后的有效因素,方法：对121例舌鳞癌患者进行回顾研究,应用Cox比例风险模型对13个与舌鳞癌术后生存率有关的指标进行回归分析。结果：只有原发灶的切缘状态和颈淋巴结转移情况才是影响舌鳞癌术后生存率的独立指标（P＜0．05）,结论：对原发灶和颈转移淋巴组织的彻底手术切除是提高舌鳞癌患者生存率的关键。     

舌鳞状细胞癌并发系统性红斑狼疮1例

临床资料：患者,女,13岁。因“舌痛,舌右缘溃疡4月余”就诊于我院口腔内科。门诊行舌部溃疡病理切片检查,诊断为舌右缘鳞状细胞癌（Ⅰ级）,收入院。1月余前患“伤寒”,已治愈;并同时确诊系统性红斑狼疮。体检：面色晦暗,双侧面颊部、颈部、左上臂及背部皮肤散在分布暗红色斑丘疹,压之褪色,无压痛,无明显浸润感,表面未见糜烂。     

口腔鳞状细胞癌基底膜表达与浸润厚度的关系

目的 探讨肿瘤基底膜表达在评判肿瘤临床病理方面的作用。方法 对51例口腔鳞状细胞癌基底膜表达及浸润厚度进行研究。结果 口腔鳞状细胞癌基底膜表达异常，出现不同程度缺损，甚至完全消失，统计分析发现，基底膜缺损越多，肿瘤浸润厚度越大。结论 基底膜在防止肿瘤浸润方面有着重要作用，肿瘤基底膜表达程度对评价肿瘤生物学特性有着重要意义。     

影响舌鳞状细胞癌预后的临床病理因素分析

目的 研究影响舌鳞状细胞癌预后的临床病理因素。方法 以TNM分类,病理分级,淋巴细胞浸润程度,浸润厚度,病理核分裂数,微血管数等指标来研究与舌鳞状细胞癌预后有关的因素。结果 分化程度,淋巴细胞浸润程度,浸润厚度,微血管数,病理核分裂数与区域淋巴结转移无关,肿瘤大小与区域淋巴结转移有关,肿瘤大小,淋巴细胞浸润程度,微血管数等指标与术后复发转移无关,浸润厚度,分化程度,病理核分裂数与术后复发转移有关。结论 对于肿瘤直径大的舌鳞状细胞癌应积极采取颈淋巴结清扫术;分化程度低者易出现术后复发转移;舌鳞状细胞癌浸润厚度超过6mm时,病理核分裂数大于2个／税镜视野时,易出现术后复发转移。     

The accuracy of magnetic resonance imaging to measure the depth of invasion in oral tongue cancer: a systematic review and meta-analysis

The accuracy of magnetic resonance imaging (MRI)-derived depth of invasion (DOI) compared to histopathological DOI is still controversial. A meta-analysis was performed to address this controversy and further investigate the best imaging sequence to measure DOI of tongue squamous cell carcinomas (SCC). A comprehensive literature search of five electronic databases was conducted. Stata/SE was used to establish a continuous variable model to assess the consistency between MRI-derived DOI and histopathological DOI. IBM SPSS Statistics 22.0 was used to evaluate the correlation between MRI-derived DOI and histopathological DOI. The meta-analysis showed that the weighted mean difference (WMD) of DOI measured by MRI had an acceptable overestimation compared with that measured by histopathology (WMD 1.64 mm; P < 0.001). In the subgroup analyses, there was no difference between T1-weighted imaging (T1WI) and histopathological values (WMD 0.77 mm; P = 0.273), while T2-weighted imaging (T2WI) had a major overestimation (WMD 2.09 mm; P < 0.001). The overall inter-class correlation coefficient (ICC) between MRI-derived DOI and histopathological DOI was 0.869 (95% CI 0.837–0.895), and was 0.923 (95% CI 0.894–0.944) in the T1WI subgroup and 0.790 (95% CI 0.718–0.845) in the T2WI subgroup. MRI is an accurate modality for evaluating the DOI in oral tongue SCC, and T1WI showed relatively higher validity than T2WI for DOI measurements.     

RhoE在舌鳞状细胞癌中的表达及其对细胞迁移和侵袭的影响

目的初步探讨RhoE在舌鳞状细胞癌(TSCC)中的表达水平,及其对TSCC细胞迁移和侵袭的影响及相关机制。方法选取在青岛大学附属青岛市市立医院口腔医学中心颌面外科2017—2019年手术切除的TSCC原发灶48例,采用免疫组织化学法检测标本(TSCC组织、癌旁组织)中RhoE的表达水平,并提取标本的RhoE mRNA和蛋白进一步检测RhoE的表达水平。选取TSCC SCC-4和CAL-27细胞系进行体外实验,采用瞬时转染法过表达RhoE,并应用实时荧光定量PCR (qRT-PCR)及蛋白质免疫印记法检测过表达效率;细胞划痕实验及Transwell细胞侵袭实验分别检测TSCC细胞的迁移和侵袭能力;蛋白质免疫印记法检测相关蛋白Rho关联含卷曲螺旋结合蛋白激酶1 (ROCK1)、基质金属蛋白酶2 (MMP-2)、基质金属蛋白酶9 (MMP-9)的表达水平;采用GraphPad Prism 8.2.1软件对实验数据进行统计学分析。结果 TSCC组织中RhoE的表达水平显著低于癌旁组织(P<0.05);在体外实验中,与空白对照组和阴性对照组相比,过表达实验组TSCC细胞的迁移和侵袭能力显著...     

体外沉默法尼基转移酶对舌鳞状细胞癌迁移和侵袭的影响

目的 通过RNA干扰沉默法尼基转移酶（FTase）,探讨沉默FTase对舌鳞状细胞癌迁移和侵袭的影响及其相关机制。方法 针对FTase设计并构建3条小干扰RNA（siRNA）,转染人舌鳞状细胞癌CAL27和SCC-4细胞（实验组）,同时设置阴性对照组（转染NC-siRNA）和空白对照组（不转染siRNA）。应用实时荧光定量PCR检测各组细胞FTase、HRAS的mRNA表达,根据FTase mRNA的表达量,选取沉默效率最高的FTase-siRNA转染组作为进一步研究的实验组。蛋白质免疫印迹法检测各组细胞FTase、HRAS、p65、p-p65(S536)、基质金属蛋白酶9（MMP-9）、低氧诱导因子1α（HIF-1α）、血管内皮生长因子（VEGF）的蛋白表达,Transwell侵袭实验和细胞划痕实验检测各组细胞的侵袭和迁移能力。结果 与阴性对照组和空白对照组相比,实验组FTase的mRNA和蛋白表达下降（P<0.05）,HRAS的mRNA和蛋白表达无明显变化（P>0.05）,p-p65(S536)、MMP-9、HIF-1α、VEGF蛋白表达下降（P<0.05）,p65蛋白表达无明显变化（P>0.05）;实验组的细胞侵袭和迁移能力降低（P<0.05）。结论 体外沉默舌鳞状细胞癌细胞FTase,可以抑制细胞体外迁移和侵袭能力,为FTase可能作为舌癌治疗的分子靶点提供了一定的理论和实验依据。     

Tranexamic acid can inhibit tongue squamous cell carcinoma invasion in vitro

Objectives:  Tranexamic acid (TA) is an inhibitor of plasminogen activation commonly used in surgery. Plasmin, the end product of plasminogen activation, degrades fibrin in the thrombus, leading to thrombolysis. However, plasmin is also associated with progression of several cancers and with cancer-associated matrix metalloproteinase-9 (MMP-9) activation. As the gelatinases MMP-2 and -9 are involved in cancer progression, several antigelatinolytic drugs have been developed as potential anticancer therapeutics. We previously developed gelatinases targeting peptide CTT1 capable of inhibiting carcinoma growth.
Study design:  The effects of TA and CTT1 on tongue carcinoma aggressiveness were evaluated in an in vitro assay of human HSC-3 and SCC-25 cells.
Materials and methods:  The cells were cultured with or without TA and CTT1 and their proMMP-9 production and activation were analysed with Western immunoblotting and gelatin zymography. Their effects on tongue carcinoma invasion were analysed in a Matrigel assay.
Results:  Tranexamic acid alone and in combination with CTT1 can inhibit tongue SCC invasion in vitr o, at least partially explained by its property of reducing the plasmin-mediated activation of proMMP-9.
Conclusions:  These data suggest that patients undergoing surgical therapy for large oral malignancies may cobenefit from prolonged TA therapy, because of its antithrombolytic and antitumour properties.     

Can radiological examination of mandibular bone invasion accurately predict the need for mandibular resection in oral squamous cell carcinoma?

Bone invasion by oral squamous cell carcinoma necessitates jaw resection, with preoperative imaging ideally able to guide the resection. A retrospective review of 109 patients with oral squamous cell carcinoma who underwent mandibular resection was performed. Eighty-three had preoperative computed tomography (CT) imaging and 72 underwent magnetic resonance imaging (MRI). The presence of bone invasion on imaging was compared to histopathology. Bone invasion was detected in 44 of 109 resection specimens (40.4%) and was identified on CT in 31 of 83 cases (37.4%) and on MRI in 35 of 72 cases (48.6%). The sensitivity and specificity of CT for detecting bone invasion was 69.0% and 79.6%, respectively, while for MRI was 87.1% and 80.5%, respectively. Histological detection of bone invasion was associated with greater disease-specific mortality (P = 0.002), as was MRI detection of bone invasion (P = 0.027). CT detection was not significant (P = 0.240). Negative prediction of bone invasion was 95% accurate for both modalities in clinically non-invaded mandibles. Survival was reduced in patients who underwent marginal mandibular resection when bone invasion was detected histologically (33.3% vs. 70.5%, P = 0.277) and with CT, although this was not statistically significant. More data are required to determine whether more aggressive resection is warranted when bone invasion is detected preoperatively.     

The importance of stromal inflammation in squamous cell carcinoma of the tongue

J Oral Pathol Med (2012) 41 : 379–383 Background: Histological risk assessment evaluating worst pattern of tumour invasion (WPOI), and lymphocytic response (LR), has previously been shown to be of prognostic significance in squamous cell carcinomas of the head and neck (SCCHN). SCCHN is a heterogeneous group of tumours including tumours located in the oral cavity, of which the majority is located in the tongue. Methods: Haematoxylin/eosin–stained slides from diagnostic biopsies from 94 cases of SCC on the tongue were evaluated for WPOI and LR. Within the inflammatory infiltrate, the percentage of eosinophilic granulocytes was also estimated. Results were correlated with clinical data such as response to treatment and recurrence. Results: For WPOI the majority of patients, 84%, showed small invasive tumours islands with a size <15 cells (grade 4). No correlation with survival, response to treatment or recurrence was seen for WPOI. More than half of the patients showed a dense lymphocytic infiltrate, a factor that was significantly correlated with complete response to radio therapy. Of the patients with dense lymphoid infiltrate, the majority, 63%, did not either have a recurrence. No significant correlation with recurrence, response to treatment or any other factor was seen for presence of eosinophils. Conclusions: Data clearly showed that tongue tumours have a split invasive growth pattern and an intense inflammatory response at the tumour interface. Results also indicated that evaluation of the intensity of the inflammatory infiltrate at the tumour interface in tongue SCC could provide information of potential importance for choice of treatment and prognosis.     

口腔鳞癌脉管侵袭的研究

对４４例口腔鳞癌组织切片用抗血型抗原ＡＢＨ抗体作免疫组化染色，以研究癌组织对脉管的侵袭。结果表明，该方法为脉管内皮细胞提供了清晰的染色，显示脉管侵袭的阳性率为４０．９％，显著高于以Ｈ·Ｅ染色显示的阳性率（２０．５％）。口腔鳞癌组织侵袭脉管与其分化程度、生长方式及淋巴结转移有密切关系。作者提示口腔鳞癌脉管侵袭的研究对判断预后有重要意义