Anatomical map of the cranial vasculature and sensory ganglia

There is growing evidence of a direct influence of vasculature on the development of neurons in the brain. The development of the cranial vasculature has been well described in zebrafish but its anatomical relationship with the adjacent developing sensory ganglia has not been addressed. Here, by 3D imaging of fluorescently labelled blood vessels and sensory ganglia, we describe for the first time the spatial organization of the cranial vasculature in relation to the cranial ganglia during zebrafish development. We show that from 24 h post‐fertilization (hpf) onwards, the statoacoustic ganglion (SAG) develops in direct contact with two main blood vessels, the primordial hindbrain channel and the lateral dorsal aortae (LDA). At 48 hpf, the LDA is displaced medially, losing direct contact with the SAG. The relationship of the other cranial ganglia with the vasculature is evident for the medial lateral line ganglion and for the vagal ganglia that grow along the primary head sinus (PHS). We also observed that the innervation of the anterior macula runs over the PHS vessel. Our spatiotemporal anatomical map of the cranial ganglia and the head vasculature indicates physical interactions between both systems and suggests a possible functional interaction during development.     

Morphologic changes of the cranial nerves, spinal cord ganglia and roots in botulism

Peripheral nervous system was studied morphologically in 4 patients who had died from botulism. The results indicate considerable alterations in cranial and spinal nerves as well as in spinal cord ganglia. This may explain a neurological symptoms (radiculoneuritis, radiculoganglionitis) in botulism.     

Differential contribution of Neurog1 and Neurog2 on the formation of cranial ganglia along the anterior-posterior axis

Background: The neural crest (NC) and placode are transient neurogenic cell populations that give rise to cranial ganglia of the vertebrate head. The formation of the anterior NC‐ and placode‐derived ganglia has been shown to depend on the single activity of either Neurog1 or Neurog2. The requirement of the more posterior cranial ganglia on Neurog1 and Neurog2 is unknown. Results: Here we show that the formation of the NC‐derived parasympathetic otic ganglia and placode‐derived visceral sensory petrosal and nodose ganglia are dependent on the redundant activities of Neurog1 and Neurog2. Tamoxifen‐inducible Cre lineage labeling of Neurog1 and Neurog2 show a dynamic spatiotemporal expression profile in both NC and epibranchial placode that correlates with the phenotypes of the Neurog‐mutant embryos. Conclusion: Our data, together with previous studies, suggest that the formation of cranial ganglia along the anterior‐posterior axis is dependent on the dynamic spatiotemporal activities of Neurog1 and/or Neurog2 in both NC and epibranchial placode. Developmental Dynamics 241:229–241, 2012. © 2011 Wiley Periodicals, Inc.     

The blocking effect of cholinolytic substances at the parasympathetic ganglia

Summary The most authentic data on the blocking effect of cholinolytics on the parasympathetic ganglia may be obtained only when different method, including experiments on the urinary bladder, are employed.Presented by Active Member Acad. Med. Sci. USSR, S.V. AnichkovPresented at the 646th session of the I.M. Sechenov Physiological Society, April 17, 1956.     

Anatomical basis for the surgical approach to the dental roots

Summary A radioanatomical study of serial cuts in the frontal and sagittal planes leads to a better appreciation of the elementary precautions which should be taken during surgery to the dental apices.

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Bases anatomiques de l'abord chirurgical des racines dentaires

Résumé La confrontation anatomo-radiologique de coupes sériées frontales et sagittales permet de mieux apprécier les précautions élémentasires à observer lors de l'abord chirurgical de l'apex des dents.

     

Anatomical contribution to the surgical construction of the sartorius muscle flap

The sartorius muscle (SM) is frequently used as a surgical flap. This study intends to describe sartorius nerve and artery distribution in adult men. Fifty-three specimens obtained from fresh cadavers were prepared as described: 32 specimens were injected with a red-colored gelatin solution through the femoral artery so that intra-muscular arteries and nerves were dissected; six specimens were injected with barium sulfate solution through the femoral artery for radiography; seven specimens were injected with a Chinese ink solution, also through the femoral artery, for diaphanization; seven specimens were injected with a solution of vinyl acetate, through the femoral artery, to obtain an arterial cast and one specimen was cut and colored by Masson’s Trichrome. Sartorius branching patterns of the nerve and artery were schematized. The following measurements were taken for each dissected muscle: total length, arterial pedicle length and distance between each arterial pedicle to the proximal muscle extremity. Five to nine arterial pedicles were found in the sample. In their trajectories, these arteries may give rise to many branches to form a dense and elongated net of anastomoses. Intra- and inter-pedicular anastomoses were observed in the inner part of the muscle. The nerve supply originated from one or two branches, which enter the muscle together with the first or second arterial pedicle. The nerve branches were divided into two or three territorial branches, and then into four or five segmental branches, running longitudinally inside the muscle. The muscles showed an average length of 44.81 cm. SM is a segmented structure and it can be divided into as many as five arterial and nervous segments. In the proximal and middle parts, the muscle has better arterial supply. The segments can be filled by adjacent pedicles, due to an elongated net of anastomoses, which allow a longer arc of rotation in the construction of pedicled flaps.     

Pediatric patient education: the unanswered challenge to medical education

As many as 10-20% of pediatric patients in this country have a chronic illness. This number will likely increase. Unfortunately, there has not been a parallel growth in physicians acquiring knowledge and skills in patient education. The disparity between these high tech and high touch skills is the most logical explanation why the morbidity in some chronic illnesses like asthma has not improved despite recent advances in diagnosis and treatment. The discussion focuses on the barriers to patient education and why it has not been a more integral part of caring for chronically ill children and their families. The solutions to this problem are complex but must include the willingness of health care professionals to welcome patients and their families as partners in the process. In addition, there is a need for change in the medical education system so that patient education is taught as a continuum, beginning with principles and theory and concluding with application to patient care.     

Anatomical significance of the nerve to the pyramidalis muscle: A morphological study

Characteristics of the nerve to the pyramidalis muscle (NPy), including its origin, course and distribution, were observed (macroscopically) in detail in the present study. The spinal segments that give rise to the nerve vary considerably and involve Th12-L2. The course and distribution of the nerve also vary widely. The NPy is given off from one of the following: (i) the anterior cutaneus branch (Rca) of the intercostals nerve; (ii) the ilioinguinal (li) nerve; or (iii) the genital branch (Rg) of the genitofemoral nerve. The NPy can be classified into nine types according to features of the course and branching pattern of the Rca and li. In three of 67 cases, the pyramidalis muscle had two nerves. Double-innervated pyramidalis muscles received one nerve from a transitional-type Rca (Rcat) and a second nerve derived from one of the superficial Rca (Rcas), li or Rg. The NPy derived from the deep Rca (Rcap; Type 1), Rcat (Type 2) and containing their features as well as the Type 9 (Rcat + li + Rg) reach the muscle from behind. Types 3-8 (not containing features of the Rcap and Rcat) enter the muscle from its surface. The branch that gives off the NPy is determined by the level of segmental origin, with the segmental origin of branches from the Rca (Types 1-4), li (Types 5-6) and Rg (Types 7-9) getting lower in that order. The level of segmental origin of the NPy derived from different Rca becomes lower in the following order: Rcap (Type 1), Rcat (Type 2), Rcas (Type 3), Rcas' (Rcas entering the inguinal canal; Type 4). When the origin of the NPy is from a lower segment, the origin of the boundary nerve (Rcap/Rcas) is also deviated downward. The changes in the NPy are related to the deviation of the entire lumbar plexus.     

A contribution to the study of the accessory nerve: Surgical implications

Summary The anatomy of the accessory n. is defined in the light of 100 dissections with particular regard to the following aspects: its relations with the internal jugular v.; its participation in the innervation of the sternocleidomastoid m.; its location in relation to the anterior and posterior borders of that muscle and to the anterior border of the trapezius; and its branching and anastomoses in the supraclavicular fossa.

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Contribution à l'étude du nerf accessoire et incidences chirurgicales

Résumé Cent dissections pratiquées au Laboratoire d'Anatomie tentent de contribuer à préciser l'anatomie du n. accessoire (n. spinal) et plus particulièrement: ses rapports avec la v. jugulaire interne; sa participation à l'innervation du m. sterno-cléido-mastoïdien; son repérage par rapport aux bords antérieur et postérieur de ce muscle, et au bord antérieur du trapèze; sa ramescence et ses anastomoses dans le creux sus-claviculaire.

     

Localization of neurons giving rise to the oculomotor parasympathetic outflow: A HRP study in cat

Localization of neurons giving rise to preganglionic fibers to the ciliary ganglion was attempted in the cat, utilizing retrograde axonal transport of horseradish peroxidase (HRP). After injection of HRP into the oculomotor nerve root at the level of the interpeduncular fossa, a few neurons of the Edinger-Westphal nucleus (EW) were labeled with HRP rostrally within the anteromedian nucleus (AM);HRP-labeled EW-neurons were rarely seen caudally within the visceral nucleus (VN). Other possible preganglionic neurons labeled with HRP were distributed mainly in rostromedial tegmental areas close to the lateral border of the AM, and in rostroventral areas of the mesencephalic central gray.     

Synaptic transmission in parasympathetic ganglia in the urinary bladder of the cat.

1. Electrophysiological techniques were used to study the sacral para-sympathetic input to pelvic ganglia located on the surface of the urinary bladder of the cat. 2. Synaptic transmission in pelvic ganglia was mediated primarily via nicotinic receptors although muscarinic excitatory receptors were present. 3. The most prominent characteristic of transmission in pelvic ganglia was the marked recruitment elicited by increasing frequencies of preganglionic nerve stimulation. Post-ganglionic action potentials were of low amplitude at low frequencies of stimulation (0-1-0-5c/s), but commonly increased to five to twenty times control amplitudes during continuous stimulation at frequencies between 5 and 10c/s. Thus, it is proposed that vesical ganglia may act as "filters" in the micturition pathway; blocking the excitatory input to the bladder when intravesical pressure and parasympathetic firing is low and facilitating the neural input to the bladder during micturition when preganglionic activity is high. 4. Information was also obtained about the characteristics of the parasympathetic post-ganglionic neurones innervating the bladder. Stimulation of the preganglionic fibres in the pelvic nerve elicited a bimodal contraction consisting of an initial phasic response, which was atropine-resistant and a tonic response which was blocked by atropine. This suggests that two types of neurones, cholinergic and non-cholinergic, may mediate the sacral input to the vesical smooth muscle.     

Contributions of placodal and neural crest cells to avian cranial peripheral ganglia

The method of embryonic tissue transplantation was used to confirm the dual origin of avian cranial sensory ganglia, to map precise locations of the anlagen of these sensory neurons, and to identify placodal and neural crest-derived neurons within ganglia. Segments of neural crest or strips of presumptive placodal ectoderm were excised from chick embryos and replaced with homologous tissues from quail embryos, whose cells contain a heterochromatin marker. Placode-derived neurons associated with cranial nerves V, VII, IX, and X are located distal to crest-derived neurons. The generally larger, embryonic placodal neurons are found in the distal portions of both lobes of the trigeminal ganglion, and in the geniculate, petrosal and nodose ganglia. Crest-derived neurons are found in the proximal trigeminal ganglion and in the combined proximal ganglion of cranial nerves IX and X. Neurons in the vestibular and acoustic ganglia of cranial nerve VIII derive from placodal ectoderm with the exception of a few neural crest-derived neurons localized to regions within the vestibular ganglion. Schwann sheath cells and satellite cells associated with all these ganglia originate from neural crest. The ganglionic anlagen are arranged in cranial to caudal sequence from the level of the mesencephalon through the third somite. Presumptive placodal ectoderm for the VIIIth, the Vth, and the VIIth, IXth, and Xth ganglia are located in a medial to lateral fashion during early stages of development reflecting, respectively, the dorsolateral, intermediate, and epibranchial positions of these neurogenic placodes.     

The fine structure of parasympathetic nerve cells in the otic ganglia of the rabbit

The ultrastructure of parasympathetic neurons in the otic ganglia of the rabbit is described with the object of providing a basis for comparisons of parasympathetic with sympathetic nerve cells. The neuronal cytoplasm contains Nissl substance, mitochondria, dense bodies, agranular Golgi membranes, neurofilaments (60 to 100 Å in diameter) and microtubules having diameters about 200 Å and filled with irregularly dense material. Granulated vesicles (about 900 Å in diameter) consisting of a limiting membrane and enclosing a dense spherical droplet of about 500 Å diameter are also present in the cytoplasm and processes of these neurons and in presynaptic terminals, thus disproving the hypothesis put forward by some authors that the presence of granulated vesicles indicates a sympathetic nervous pathway. Long, tubular bodies with a dense central core are described which are possibly peculiar to parasympathetic neurons. The nuclear membrane is double, porous and sometimes exhibits localized, deep invaginations of its surface. Junctional complexes are described between apposing membranes of neurons and satellite cells which are morphologically similar to zonulae adhaerentes. Small gaps exist in the cytoplasmic sheath surrounding the neurons. Over these areas only a basement membrane separates the nerve cell from the connective tissue space and the significance of this finding is discussed.