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目的 探讨膜联蛋白A7在人宫颈鳞癌和正常组织中的表达情况. 方法 应用免疫组织化学SP法,检测人宫颈鳞癌和正常宫颈组织蜡块各25例中膜联蛋白A7的表达差异;Western .blotting和半定量RT-PCR法检测人宫颈鳞癌和正常组织新鲜标本各25例中膜联蛋白A7的表达差异. 结果 膜联蛋白A7在组织中蛋白和mRNA水平表达一致,均为宫颈鳞癌组织表达高于正常宫颈组织,差异有统计学意义. 结论 膜联蛋白A7在人宫颈鳞癌表达上调, 可能成为宫颈癌诊断的一个新的指示靶点. 相似文献
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目的 检测膜联蛋白A7(ANXA7)在胃癌组织中的表达改变,探讨胃癌的发生机制。 方法 收集人胃癌组织55例及正常胃组织25例,细胞裂解液裂解组织细胞,蛋白定量试剂盒进行蛋白定量后,Western blotting法和Real-time PCR法分别于蛋白水平和基因转录水平检测ANXA7在胃癌组织和正常胃组织中的表达改变,并用免疫组织化学法对ANXA7在细胞中的定位和表达进行检测。 结果 ANXA7在胃癌中蛋白水平和转录水平的表达均显著增高,与正常组相比差异有显著性(P<0.05); ANXA7在胃癌细胞中定位于细胞质。 结论 膜联蛋白A7在胃癌中的表达增高有可能是胃癌发生的机制之一。 相似文献
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E-Cadherin在子宫颈上皮内瘤变及子宫颈鳞癌中的表达 总被引:1,自引:0,他引:1
目的:了解E-Cadherin在子宫颈上皮内瘤变及子宫颈鳞癌的表达及其临床意义。方法:采用S-P免疫组化方法对20例慢性子宫颈炎,40例子宫颈上皮内瘤变及71例浸润性子宫颈鳞癌进行E-Cadherin检测。结果:慢性子宫颈炎、CIN I、CINⅡ、CINⅢ、浸润性子宫颈鳞癌、癌旁组织E-Cadherin的异常表达率分别为40.0%、23.1%、16.7%、60.0%、95.8%及53.8%。子宫颈鳞癌E-Cadherin的异常表达率明显高于子宫颈上皮内瘤变及癌旁组织。结论:CINⅢ、浸润型子宫颈鳞癌E-Cadherin表达下调或缺失。 相似文献
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目的:探讨热休克蛋白(heat shock proteins,HSPs)家族中HSP27、HSP70、HSP90α在子宫颈鳞癌中的表达及其意义。方法:应用免疫组化S-P法观察41例子宫颈鳞癌和10例慢性子宫颈炎组织中HSP27、HSP70、HSP90α的表达。结果:HSP27、HSP70、HSP90α在子宫颈鳞癌中的阳性表达率分别为46.3%、70.7%、80.4%,在慢性子宫颈炎组织中的阳性表达率分别为10.0%、30.0%、20.0%,子宫颈鳞癌与慢性子宫颈炎之间差异有显著性(P<0.05)。随着子宫颈鳞癌病理分级和临床分期的升高,HSP27表达逐渐降低,而HSP70、HSP90α表达逐渐增加(P<0.05)。结论:HSP27、HSP70、HSP90α在子宫颈鳞癌的发生发展过程中可能扮演着重要的角色,HSP27主要参与子宫颈鳞癌的早期发生。 相似文献
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膜联蛋白A7低表达对人肝癌HepG2细胞增殖的影响 总被引:1,自引:1,他引:0
目的 探讨膜联蛋白A7低表达对人肝癌HepG2细胞的增殖产生的影响。方法 采用Western blotting法鉴定siRNA可有效抑制膜联蛋白A7表达,然后用脂质体转染法将siRNA转染入HepG2细胞,将细胞分为siRNA干扰组、阴性对照组和空白对照组,在转染后24h、48h、72h进行细胞计数以绘制细胞增殖曲线,转染后48h进行MTT实验以检测细胞增殖活力;免疫组织化学法检测cyclinD1和Ki67的表达,Western blotting法检测cyclinD1的表达情况。结果 转染了靶向膜联蛋白A7的siRNA后48h的HepG2细胞,细胞计数和MTT实验可见siRNA干扰组细胞增殖活力较阴性对照组和空白对照组显著降低(P<0.05);cyclinD1和Ki67蛋白表达均为siRNA干扰组细胞表达显著低于两对照组(P<0.05)。 结论 膜联蛋白A7低表达可能对HepG2细胞的增殖具有一定的抑制作用。 相似文献
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目的 检测膜联蛋白A5(ANXA5)在人宫颈鳞癌患者外周血中的含量改变,探讨ANXA5作为宫颈癌诊断标志物的可行性.方法 收集人宫颈鳞癌患者外周血清48例和正常育龄妇女外周血清38例,低速离心取上清,采用酶联免疫吸附测定(ELISA)法分别检测血清中ANXA5和鳞状细胞癌抗原(SCCAg)的含量;离心后取红细胞行RT-PCR进行患者与正常组ANXA5表达的检测.组间检验采用t检验;并进行肿瘤患者中ANXA5与SCCAg的相关性分析.结果 宫颈鳞癌患者血清中ANXA5的含量均比正常人显著增多,血细胞中ANXA5 mRNA的表达显著增多,差异有显著性(P<0.05);SCCAg在肿瘤组中的含量同样显著高于正常组(P<0.05);ANXA5与SCCAg具有明显的相关性,但ANXA5的阳性率大于SCCAg.结论 ANXA5在宫颈鳞癌患者外周血中含量增高,特异性和敏感性较高,可作为临床诊断和预后宫颈鳞癌的备选标志物. 相似文献
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目的观察骨桥蛋白(osteopontin,OPN)在子宫颈鳞癌中的表达及其临床意义,评估OPN是否可以作为子宫颈鳞癌的诊断和预后标记。方法采用免疫组化SP法对88例子宫颈鳞癌及18例正常子宫颈组织中OPN进行检测,并复习相关文献。结果免疫组化染色结果示OPN在子宫颈鳞癌的阳性率为59.09%(52/88),在18例正常子宫颈组织均阴性。子宫颈鳞癌组OPN阳性表达与子宫颈鳞癌的淋巴结转移(P=0.030)和无病生存时间有关(P=0.029),与患者年龄、子宫颈鳞癌浸润的深度、FIGO分期(Ⅰ、Ⅱ期)及病理分级无关(P>0.05)。结论 OPN在子宫颈鳞癌的转移、发展过程中扮演着重要角色,在治疗过程中对其进行检测有助于对子宫颈鳞癌患者的预后进行评估。 相似文献
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膜联蛋白A5低表达对人宫颈癌细胞系HeLa细胞增殖与凋亡的影响 总被引:2,自引:2,他引:0
目的 观察宫颈癌细胞系HeLa细胞在膜联蛋白A5表达降低后,细胞增殖和凋亡的情况. 方法 采用BNA干扰的方法,通过免疫印迹法筛选出对HeLa细胞中膜联蛋白A5的表达抑制效率最高的siRNA,脂质体法将siRNA转染入细胞48h后,用MTT法检测细胞增殖的改变情况,用细胞凋亡光学检测试剂盒检测细胞的凋亡.结果 Helm细胞中膜联蛋白A5的表达被显著抑制,其增殖速度与未转染siRNA的普通HeLa细胞相比,无显著性差异;细胞凋亡显著减少,与未转染siRNA的普通HeLa细胞相比,有显著性差异. 结论 膜联蛋白A5低表达对宫颈癌细胞系HeLa细胞的增殖未见显著影响,但可能影响了细胞的凋亡. 相似文献
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CHRISTEL HEROLD-MENDE SIMONE SEITER ANTONIO I. BORN ERIK PATZELT MICHAEL SCHUPP JOACHIM Z
LLER FRANZ X. BOSCH MARGOT Z
LLER 《The Journal of pathology》1996,179(1):66-73
Splice variants of the adhesion molecule CD44 have been described as essential for the lymphatic spread of rat tumour cells and are claimed to be involved in the metastatic spread of several human tumours. Immunohistochemistry has been used to analyse the expression pattern of CD44 standard (CD44s) and variant (CD44v) isoforms in normal and dysplastic squamous epithelia, as well as in primary and metastatic squamous cell carcinomas (SCCs), which spread predominantly by way of the lymphatic system. Frozen sections of squamous epithelia and of squamous cell carcinomas were stained with a panel of monoclonal antibodies recognizing epitopes of CD44s as well as of the variant exons v5, v6, v7, v7–v8, and v10. The stratum basale and stratum suprabasale of squamous epithelia stained with all antibodies; the stratum spinosum stained with anti-CD44v5, anti-CD44v6, anti-CD44v7–8 and anti-CD44v10; the lower layers of the stratum corneum stained with anti-CD44v5. This expression profile was seen in epithelia of the lip, the tongue, the gingiva, the hard palate, the floor of the mouth, the buccal mucosa, and the pharynx. The same pattern of expression was also noted in dysplastic epithelia, but expression of the variant exons v7, v8, and v10 was significantly downregulated in primary squamous cell carcinomas and was not detected at all in the majority of metastasis-derived specimens. Expression of CD44v5 and CD44v6, on the other hand, was mainly unaltered. Thus, epithelial cell layers representing different stages of differentiation express distinct sets of CD44 variant isoforms, where especially exons v8–v10 might be required for the maintenance of the structural integrity of squamous epithelium. Downregulation of these exons on tumour cells could indicate that they are irrelevant for tumour progression or may even hamper infiltration of surrounding tissue or of lymphatics. 相似文献
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CHRISTINE GILLES MYRIAM POLETTE JACQUES PIETTE ANNE-CATHERINE DELVIGNE ERIK W. THOMPSON JEAN-MICHEL FOIDART PHILIPPE BIREMBAUT 《The Journal of pathology》1996,180(2):175-180
Vimentin is an intermediate filament protein normally expressed in mesenchymal cells, but evidence is accumulating in the literature which suggests that the aberrant expression of vimentin in epithelial cancer cells might be related to local invasiveness and metastatic potential. Vimentin expression has previously been associated with invasive properties in an in vitro model consisting of a set of HPV-33-transformed cervical keratinocyte cell lines.1,2 In the present study, in order to emphasize those in vitro findings, the expression of vimentin has been investigated in cervical neoplasms of different grades, using immunohistochemistry. A clear association is reported between vimentin expression and metastatic progression, since vimentin was detected in all invasive carcinomas and lymph node metastases, but not in CIN III lesions. These in vivo results are compared with present and previous data obtained in vitro on cervical keratinocyte cell lines, where vimentin expression also correlated with in vitro invasiveness. 相似文献
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人外周血树突状细胞与人肺鳞癌细胞的融合研究 总被引:2,自引:1,他引:2
目的 探讨用杂交瘤技术制备树突状细胞疫苗的方法。研究树突状细胞,肿瘤细胞之融合细胞的形态及表型特征。方法 用PEC法将免疫磁珠法分离获得的人外周血树突状细胞与人肺鳞癌细胞株LTEP78融合,瑞氏一姬姆萨染色观察树突状细胞、LTEP78细胞及其融合细胞的形态结构,SP免疫细胞化学染色法检测融合细胞的分子表达。结果 分离的树突状细胞能高表达HIJA—DR分子,而LTEP78细胞则不表达;将树突状细胞与ITEP78细胞按10:1比例融合后,两者的细胞膜、细胞质依次融合,获得的融合细胞仍能表达HLA-.DR分子。结论人树突状细胞与人肺鳞癌细胞的融合是一个动态过程,通过将两者融合,人肺癌细胞获得了人树突状细胞表达的HLA-DR分子,从而增强了其免疫原性,为人树突状细胞疫苗的研制奠定了基础。 相似文献
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口腔鳞癌中c-erbB2和p53基因的扩增和表达 总被引:1,自引:0,他引:1
目的探讨c-erbB2和p53基因扩增和表达在口腔鳞癌(OSCC)发生发展中的作用. 方法用原位杂交和免疫组织化学技术与图像分析相结合的方法,对30例口腔鳞癌和5例正常口腔粘膜中c-erbB2和p53两种基因的扩增、mRNA表达和蛋白表达状况进行了定性、定位、定量研究. 结果 OSCC中c-erbB2和p53的扩增阳性率分别为46.67%和40%;而c-erbB2和p53在OSCC中的mRNA表达率分别是70%和80%,蛋白表达率分别是46.67%和63.33%.这两种基因的扩增、mRNA表达和p53蛋白表达在各种性状差异之间均没有显著性差异(P>0.05);erbB2蛋白表达在不同部位的口腔鳞癌中具有显著性差异(P<0.02). 结论 c-erbB2和p53两种基因的扩增和过表达在OSCC发生发展中都具有重要作用,c-erbB2蛋白表达增高在腭癌和口底癌的形成过程中具有更重要的意义. 相似文献
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KAORU KUSAMA SEIJIRO OKUTSU AKIO TAKEDA TAKAO HIMIYA AKIRA KOJIMA YOSHIHIRO KIDOKORO LING CHU SHINKICHI IWANARI ITSURO KUDO ITARU MORO 《The Journal of pathology》1996,178(4):415-421
To examine the expression of p53 protein and gene alterations in oral epithelial lesions including epithelial dysplasias and primary squamous cell carcinomas, immunohistochemical and temperature gradient gel electrophoresis (TGGE) methods were applied to formalin-fixed and paraffin-embedded tissues. Morphologically normal mucosal epithelium stained negatively for p53 protein. Three out of 11 (27·3 per cent) epithelial dysplasias and 19 out of 57 (33·3 per cent) primary squamous cell carcinomas stained positively for p53 protein. Although more than half of the cases were positive for p53 protein in stage I, the positive cancer cases were found at other stages with variable frequency. Immunoreactive products were localized in the nucleus, especially in the basal and suprabasal layers. The analysis by TGGE revealed gene alterations in exons 5–8 in 3 out of 3 epithelial dysplasias and 17 out of 19 (89·5 per cent) primary squamous cell carcinomas which were immunohistochemically positive for p53 protein. These results suggest that p53 gene mutation may be involved in carcinogenesis in the oral squamous epithelium even in the early stage of the dysplasia–carcinoma sequence. 相似文献
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目的 旨在研究人胚胎肝干细胞移植入四氯化碳(CCl4)诱导性肝硬化大鼠的肝组织后,大鼠血清及肝组织血清白蛋白(ALB)表达的变化情况.方法 将健康雄性Wistar大鼠随机分为对照组和肝硬化组.对照组背部皮下注射橄榄油,CCl4组背部皮下注射CCl4橄榄油.连续注射6周后,肝硬化组再随机分为肝干细胞假移植组和肝干细胞移植组.对照组和假移植组在大鼠肝中叶注射细胞培养液,而移植组则在相应部位注射人胚胎肝干细胞2×106,移植4周后处死所有大鼠.右心室取血离心观察ALB的含量;肝中叶取材,免疫组织化学染色观察人表皮生长因子受体(EGFR)的表达,免疫荧光染色和免疫印迹法(Western blotting)法观察和测定肝组织ALB的表达变化.结果 对照组和肝干细胞假移植组未见人EGFR阳性表达的细胞,肝干细胞移植组则可见大量排列成环状的EGFR阳性表达的细胞.在肝干细胞移植组的血清ALB含量、免疫荧光染色及Western blotting法检测肝组织ALB表达,均明显高于假移植组,而与对照组无明显著异.结论 将人胚胎肝干细胞移植入肝硬化大鼠肝内可以长期存活,并可改善大鼠ALB的表达和分泌. 相似文献