Cytochemical studies of rosette-forming cells.

Two types of rosette-forming cells were identified: rosette-forming cells with membrane-bound receptors for sheep erythrocytes (E-RFC) and rosette-forming cells with Fc receptors (EA-RFC). In both types of lymphocytes the activity of acid phosphatase, beta-glucuronidase, non-specific esterase and the content of glycogen were studied. A significant difference was established between E-RFC and EA-RFC lymphocytes as concerns acid phosphatase activity; corresponding mean values being 72.4 +/- 7.5 respectively 44.4 +/- 4.5 (p less than 0.01). As oppose to the acid phosphatase activity the glycogen was significantly higher in EA-RFC lymphocytes (mean 67.2 +/- 6.9), as compared with E-RFC (mean 41.6 +/- 9.2). It seems to us that estimations of acid phosphatase activity may be of value in differentiating 2 populations of lymphocytes.     

Marijuana and T lymphocyte rosettes.

The effect of marijuana smoking on sheep cell rosetting properties of both early (active) and total T lymphocytes was studied in vitro. Significantly fewer active rosettes were formed by T cells from a population of 35 who appeared to be chronic marijuana smokers than the 34 controls. The late, or cold-enhanced rosettes formed by smokers and nonsmokers were similar, suggesting that similar numbers of rosette-forming T cells were present in the peripheral blood of smokers. These data suggest that marijuana smoking may affect the function of a subpopulation of T lymphocytes, identified by their capacity to form early rosettes, possibly by affecting these cell membranes.     

Autologous rosette-forming T cells and their relationship to OKT4+ and OKT8+ cells in chronic HBV infection

A percentage of human T lymphocytes forms rosettes with autologous erythrocytes and this property has been considered as a marker for post-thymic precursor suppressor cells capable of providing suppression under the influence of inducer cells. We quantitated autologous rosette-forming T cells (ARFC) in the peripheral blood of 37 patients with chronic HBV infection: 8 healthy carriers, 9 chronic persistent hepatitis (CPH-B) and 20 chronic active hepatitis (CAH-B). Two control groups were studied, one consisting of 26 healthy individuals and the other of 8 individuals with non-HBV-associated CAH. Patients with non-HBV-associated CAH had a significant reduction in the proportion of ARFC, whereas CAH-B patients fell into 2 distinct patterns, one with increased and the other with decreased proportions of ARFC. This was unrelated to the degree of biochemical activity of the disease or to degree of viral replication as defined by HBeAg status and HBV-DNA in the serum. Healthy carriers and CPH-B had no changes in ARFC. Simultaneous quantitation of OKT4 and OKT8+ cells was done and a positive correlation was found between the proportions of ARFC and the proportions of OKT8+ cells. The possible significance of this correlation and the relevance of the bimodal distribution of autologous rosette-forming cells in CAH-B are discussed.     

LYMPHOID CELLS MEDIATING TUMOR-SPECIFIC CYTOTOXICITY TO CARCINOMA OF THE URINARY BLADDER : SEPARATION OF THE EFFECTOR POPULATION USING A SURFACE MARKER

Peripheral lymphocytes from patients with urinary bladder carcinoma and controls have been separated on the basis of rosette formation with sheep erythrocytes. The fractions were tested for tumor-specific cytotoxicity. The E rosette-forming cells of purity 90% respond well in PHA-induced cytotoxicity but are totally inactive in the tumor assay. The non-E rosette-forming cells (purity 91%) give enhanced activity in the tumor-specific cytotoxicity as well as in antibody-mediated target cell lysis in a model system. These data support the notion that the effector cells in cell-mediated immunity to carcinoma of the urinary bladder are members of the nonthymus-derived population of peripheral lymphocytes.     

BAFF及其受体在多发性骨髓瘤中的表达

目的通过测定B淋巴细胞刺激因子（BLyS）及其受体在单克隆丙球蛋白血症中的表达水平,探讨BLyS及其受体与多发性骨髓瘤（MM）的关联性。方法流式细胞术对9例不同型别的MM患者及11例良性单克隆丙球蛋白血症（MGUS）患者,以及7名正常人外周B淋巴细胞表面BLyS及其3种受体BLyS受体（BAFFR）、穿膜蛋白活化物（TACI）、B细胞成熟抗原（BCMA）进行分析。并运用酶联免疫吸附试验（ELISA）对20例MM患者、23例MGUS患者、20名健康者血清BAFF水平进行检测。结果 MM、MGUS及正常对照组外周B淋巴细胞膜表面均表达BAFFR,但各组间差异无统计学意义（P〉0.05）。膜表面受体结合BAFF情况比较,各组间差异有统计学意义（P〈0.05）。MM组结合能力最强。TACI在MM及MGUS组低表达,其中MM组与正常对照组比较差异有统计学意义（P〈0.05）。胞浆BCMA高表达,MM组与正常对照组或与MGUS组比较差异均有统计学意义（P〈0.05）。血清BLyS水平MM组高于MGUS组（P〈0.05）,MGUS组高于正常对照组（P〈0.05）。结论 BLyS及其受体BAFFR、TACI、BCMA可能与MM的发病机制有关。血清BLyS可作为MM的实验室辅助诊断指标之一。     

Formation of total and stable E-rosettes by lymphocytes from patients with pulmonary tuberculosis stimulated by Con A or PPD

The percentage of total and stable E-rosette forming lymphocytes (tE-RFC and sE-RFC, respectively) were determined in the peripheral blood of patients with either active non-chronic, active chronic or inactive pulmonary tuberculosis as well as in healthy controls, both skin test positive and negative. Patients had low percentages of tE-RFC but increased values of sE-RFC when compared with controls. Lymphocytes cultured for 48 hr with or without 10 micrograms/ml of Con A exhibited decreased capacity to form total E-rosettes. Mitogen stimulation resulted in a very significant increase in the percentage of sE-RFC from control individuals. Although lymphocytes from tuberculous patients were able to respond to Con A stimulation, this response was significantly diminished compared to the controls. Incubation of the cells in the presence of various concentrations of PPD did not affect the formation of total E-rosettes by normal cells nor by lymphocytes obtained from tuberculous patients. Nevertheless, PPD induced a significant increase in the percentage of sE-RFC from normal tuberculin positive controls whereas lymphocytes from the same group incubated without antigen or cells from tuberculin negative controls cultured in the presence of 50 micrograms/ml of PPD did not form sE-RFC, indicating that the response might be antigen specific. Even though lymphocytes from patients with active tuberculosis formed higher numbers of stable E-rosettes than controls, they did not respond to antigen with significant increases of sE-RFC. In all the experiments it was not possible to demonstrate differences between the 3 groups of patients with various stages of the infection.(ABSTRACT TRUNCATED AT 250 WORDS)     

树突细胞介导的独特型瘤苗的体外抗骨髓瘤作用

目的研究树突细胞（DC）介导的独特型瘤苗诱导骨髓瘤抗原特异性细胞毒性T淋巴细胞（CTL）的抗肿瘤免疫反应。方法从多发性骨髓瘤（MM）患者外周血中分离获取DC前体细胞，使用GM-CSF、IL-4与TNF-α诱导分化促成熟。加入MM患者的M蛋白F（ab′），片段（Id），诱导MM肿瘤特异性CTL。光学显微镜下观察其培养过程中形态特征变化，电镜观察其超微结构，间接免疫荧光观察其表型特征，采用MTT法检测致敏DC促自体T淋巴细胞增殖的能力以及患者CTL对自体骨髓瘤细胞的特异性细胞毒杀伤作用。结果 GM-CSF、IL-4和TNF-α配伍可有效地从MM患者外周血单陔细胞中诱导出大量成熟的功能性DC。MM患者自体血清Id冲击致敏的成熟DC能够显著地提高T细胞的增殖能力，并且使幼稚T细胞活化成为肿瘤独特型CTL，各个剂量的CTLs均能够产牛针对自体MM细胞的抑制性杀伤反应。结论 在MM患者外周血中可获得典型的DC，使用负载了Id的DC疫苗能够诱导出有效的抗肿瘤免疫应答反应，以DC为基础的疫苗可能在MM免疫治疗中发挥重要的作用。     

Early activation of gammadelta T lymphocytes in patients with severe systemic inflammatory response syndrome

Innate immunity plays an important role in host defense after severe insult. gammadelta T lymphocytes are recognized as the first line of defense against microbial invasion. In this study, we evaluated gammadelta T lymphocytes in the peripheral blood of patients with severe systemic inflammatory response syndrome (SIRS), and examined on role of these cells. Thirty-seven patients with severe SIRS (SIRS criteria and serum C-reactive protein > or = 10 mg/dL) and 27 healthy volunteers were studied. Severe SIRS was caused by trauma in 14 patients (Injury Severity Score of 30.1 +/- 10.8) and by sepsis in 23 patients. The counts of gammadelta and alphabeta T lymphocytes were determined by flow cytometry of cells stained with monoclonal antibodies to gammadelta and alphabeta T lymphocyte receptors. The activation of these cells was evaluated by flow cytometry of cells stained with monoclonal antibodies to CD69 and HLA-DR. Serial counts and activation of gammadelta and alphabeta T lymphocytes were also determined in eight trauma patients (Injury Severity Score of 31.0 +/- 13.5) during a 2-week observation period. The count of gammadelta T lymphocytes in the peripheral blood of SIRS patients (30.1 +/- 6.0/microL) was significantly lower (P < 0.05) than that of the healthy volunteers (104.3 +/- 10.9/microL). The expression of CD69, an index of early activation of T lymphocytes, was significantly greater on gammadelta T lymphocytes from SIRS patients (patients 23.9% +/- 3.4%, healthy controls 4.8% +/- 0.6%, P < 0.05). In trauma patients, the expression of CD69 on gammadelta T lymphocytes increased rapidly within 48 h after injuries. In conclusion, gammadelta T lymphocytes are activated and decreased in the peripheral blood of severe SIRS patients. In trauma patients, the activation of gammadelta T lymphocytes occurs in the fairly acute phase after injuries. These results suggest a significant role for gammadelta T lymphocytes as early responders after severe insult.     

Rosette-forming ability of thymus-derived lymphocytes in humoral and cell-mediated immunity. II. Helper cell activity

The technique of velocity sedimentation at unit gravity was used to determine the size and rosette-forming ability of helper cells in nonimmune and immune C57BL/6 mice. Helper T cells were assayed by the ability to cooperate with bone barrow (B) cells in the antibody response to sheep erythrocytes (SRBC) in vivo. 19S helper cells in nonimmune animals were nonrosette-forming small lymphocytes; after immunization with SRBC in complete Freund's adjuvant, 19S helper cells were nonrosette-forming medium lymphocytes. 7S helper cells in immune animals were small lymphocytes which did not form rosettes. No SRBC binding by helper cells was observed under the conditions of rosette formation used.     

Characteristics of human large granular lymphocytes and relationship to natural killer and K cells

Recent evidence, has demonstrated an association between a subpopulation of peripheral blood mononuclear cells, morphologically identified as large granular lymphocytes (LGL), and natural killer (NK) activity. We have now evaluated more directly the role of LGL in both NK activity and antibody- dependent cellular cytotoxicity (ADCC), by using highly enriched populations of LGL, obtained by centrifugation of peripheral blood mononuclear cells on Percoll discontinuous density gradients. Both spontaneous and interferon- augmented NK and ADCC activities were exclusively associated with the LGL- enriched, low density fractions. The majority of LGL formed conjugates with NK-susceptible and antibody-coated target cells. Approximately 20 percent of small conventional lymphocytes also formed conjugates with the target cells for NK, but this was not associated with cytotoxic activity. Virtually all LGL were found to have receptors for the Fc portion of IgG (FcγR). The frequency of LGL among blood leukocytes was 2-6 percent. LGL could be enriched to an average purity of 95 percent by combining discontinuous density gradient centrifugation with subsequent adsorptions of the low density fractions on monolayers of immobilized immune complexes. About 50 percent of LGL were found to be FcγR-bearing T cells (T(G)), forming low affinity rosettes with sheep erythrocytes at 4 degrees C. Only 10-20 percent of LGL formed high affinity rosettes with sheep erythrocytes at 29 degrees C. LGL could be enriched to a purity of more than 90 percent by depleting high affinity rosette-forming cells from low density Percoll fractions. LGL were only a subpopulation of T(G) cells, because some lymphocytes with conventional morphology also adhered to the immobilized immune complex monolayers and formed high affinity rosettes with sheep erythrocytes. Separation of these cells from LGL by discontinuous density gradient centrifugation indicated that they are not cytotoxic, suggesting a morphological and functional subdivision of T(G) cells. The verification in this study that virtually all human NK and K cells have a characteristic morphology adds a useful parameter to the monitoring of human lymphocytes, and the ability to purify these cells by simple physical procedures should be invaluable in their further characterization.     

Antigen heterogeneity of human B and T lymphocytes.

Rhesus monkeys were immunized with normal human lymphoid cells, cultured lymphoid cells, and chronic leukemic lymphocytes. Antisera were analyzed by cytotoxicity and immunofluorescence techniques to study the antigenic characteristics of human lymphocytes. In an attempt to obtain a reagent specifically reactive with T (thymus-derived) lymphocytes, an antispleen antiserum was absorbed with cellf from five B- (bone marrow-derived) cell lines. After absorption, the antiserum killed 60-75% of peripheral blood lymphocytes and 40-50% of tonsil cells, so that there was a relationship between the percentage of killed cells and the proportion of T lymphocytes. However, when cells after cytotoxic treatment were assayed for rosette formation with sheep erythrocytes (a T-cell marker) 5-20% of viable rosette-forming lymphocytes were found. Therefore, this antiserum was cytotoxic for only 75-90% of T cells. From studies performed with antisera prepared against spleen and B-cell lines, we conclude that lymphoblastoid cells are antigenically different and deficient in comparison to normal B lymphocytes. In addition, cultured B-cell lines appear to be antigenically heterogenous, as shown by the cytotoxic activity remaining in antispleen and anti-B-cell lines sera after absorption with various numbers and types of lymphoid cell lines. After absorption with normal lymphocytes, an antiserum produced against chronic lymphatic leukemia cells had specific activity associated with 12 chronic lymphatic leukemia cells tested. Absorption of the same antiserum with leukemic cells from two patients showed that a certain degree of antigenic heterogeneity also exists among chronic leukemic lymphocytes.     

初诊多发性骨髓瘤患者外周血淋巴细胞亚群的检测及意义

目的:通过检测多发性骨髓瘤(multiple myeloma,MM)患者外周血淋巴细胞亚群评价MM患者机体的免疫功能状态并分析其与患者预后的相关性。方法:采用流式细胞术检测32例初诊MM患者和24例健康献血者的外周血T淋巴细胞、B淋巴细胞、NK细胞及CD4~+CD25~+调节性T细胞(CD4~+CD25~+Treg);分别采用溴甲酚绿法、透射免疫比浊法检测患者血清白蛋白(albumin,Alb)、β2-微球蛋白(β2 microglobulin,β2-MG)。结果:与健康献血者比较,MM患者外周血淋巴细胞中CD3~+、CD4~+T淋巴细胞比例无显著改变(P0.05),CD8~+T淋巴细胞、NK细胞比例升高(P0.05),CD4~+/CD8~+T淋巴细胞比值、CD19~+B淋巴细胞比例降低(P0.05),CD4~+CD25~+Treg细胞占CD4~+T淋巴细胞的比例明显升高(P0.01)。CD4~+CD25~+Treg细胞占CD4~+T淋巴细胞的比例与MM的疾病分期呈正相关,与MM患者血清中的β2-MG浓度亦呈正相关(P0.05)。结论:MM患者体内在淋巴细胞亚群的异常表达可能与其肿瘤负荷、病情进展及预后有关。CD4~+CD25~+Treg细胞的表达异常可能是MM免疫逃逸的一个重要机制。     

系统性红斑狼疮患者CD4+T淋巴细胞CD45RO/CD45RA的表达

目的：检测系统性红斑狼疮患者CD4^ T辅助淋巴细胞CD45RO和CD45RA亚群的变化及临床意义。方法：用流式细胞术检测了8例活动期SLE患者CD4^ T辅助细胞CD45RO、CD45RA的表达率，并与对照组进行了比较。结果：SLE患者CD4^ T辅助淋巴细胞CD45RO的表达率与对照组无显著性差异（59．30％vs.59.74%),CD45RA下降（21．40％vs.31.64%),CD45RD、CD45RA双阳性T辅助淋巴细胞显著增高（18．57％vs.7.92%)。结论：SLE患者体内CD45RA^ T辅助淋巴细胞被激活，向CD45RO^ 细胞转化。CD45RA^ T辅助细胞的减少是导致机体免疫功能失去平衡的重要原因。     

Lymphocyte antigens in systemic lupus erythematosus: studies with heterologous antisera.

Rabbit antisera were produced against pooled living lymphocytes from 25 patients with active systemic lupus erythematosus (SLE). Lymphocytes collected at plasmapheresis or venipuncture were frozen in liquid nitrogen and later coated with rabbit antibody to normal human tonsils and normal thymocytes immediately before intravenous immunization of rabbits. Antisera were subsequently extensively absorbed with normal human tonsillar cells, thymocytes, peripheral blood lymphocytes, erythrocytes, and leukocytes from patients with myelogeneous and lymphatic leukemia until residual base-line immunofluorescent staining of normal human lymphocytes using F(ab)2' of whole antisera averaged less than 5%. Absorbed pepsin-digested antisera detected membrane antigens which were markedly increased (mean 32%) on lymphocytes from patients with active SLE (P less than 0.05). Membrane antigens reacting with absorbed, pepsin-digested antisera were present on both T and B cells but, in most instances, predominated on T cells. Control observations using absorbed pepsin-digested antisera to normal human lymphocytes or peripheral blood lymphocytes from patients with rheumatoid arthritis showed no similar specificity. SLE patients treated with moderate or high dose corticosteroids or immunosuppressive agents (cytoxan or azathioprine) appeared to lose lymphocyte antigens detected by these reagents. Control studies with other connective tissue disease patients, miscellaneous hospitalized subjects, or normal controls showed low levels of reactivity (2-5%). SLE lymphocyte membrane antigens uniquely increased during active disease; this may represent neoantigens or alterations associated with the disease itself.     

T lymphocyte subpopulations in the bone marrow and peripheral blood of untreated patients with myeloma

Summary The quantitative distribution of the two main T lymphocyte subsets, recognizable by the ‘high’ and ‘low-affinity’ E rosette-forming cell technique of West, was studied in both the bone marrow and peripheral blood from ten untreated multiple myeloma (MM) patients. A reduced total T lymphocyte count, with a relative predominance of ‘low-affinity’ T lymphocytes (putative suppressor T cells), was found in the peripheral blood. Within a normal total T lymphoid cell count, a predominance of the T lymphocyte subset with ‘low-affinity’ characteristics was also observed in the bone marrow. An inverse correlation, that was statistically significant, was seen between the monoclonal malignant cellular B component and the ‘low-affinity’ T cell percentage in all cases. It is concluded, therefore, that such an imbalance between the ‘high’ and ‘low-affinity’ T subsets, with the latter predominating, could be of importance in the regulation of the growth rate of the monoclonal cellular B component.     

急性下呼吸道合胞病毒感染190例的淋巴细胞亚群分析

目的分析急性下呼吸道合胞病毒感染患儿的外周血淋巴细胞亚群的变化,为临床免疫调节治疗提供依据。方法对小于6个月急性下呼吸道感染住院患儿行痰病原学检测,明确为呼吸道合胞病毒为感染组。选择门诊体检儿童为对照组。同时两组病例抽外周血采用流式细胞仪检测淋巴细胞亚群值。结果感染组CD3＋、CD3＋CD8＋低于对照组,差异有统计学意义（P〈0．05）;CD3．CD19＋、CD19＋CD23＋、CD4＋／CD8＋、CD3＋CD25＋高于对照组,差异有统计学意义（P〈O．05）。感染组与对照组CD3＋CD4＋、CD16＋CD56＋差异无统计学意义（P〉0．05）。结论急性下呼吸道合胞病毒感染患儿的细胞免疫功能紊乱：T淋巴细胞受到全面抑制,B淋巴细胞激活参与病毒的清除,NK细胞比例变化不显著。     

急性髓系白血病患者T淋巴细胞内细胞因子表达特性

T淋巴细胞在抗肿瘤的免疫反应中起着重要作用，而大部分肿瘤患者往往存在免疫功能低下。本研究通过对急性髓系白血病（AML）患者T淋巴细胞胞内细胞因子特性的研究，以了解AML患者在不同状态下T淋巴细胞的功能。18例不同状态下初诊AML患者和10例健康成人外周血中的T淋巴细胞在莫能霉素存在的情况下，体外经PMA和离子霉素（ionomycin）刺激后，分别进行CD4-FITC、CD8-FITC荧光单克隆抗体染色和IFNγ-PE、IL4-PE荧光单克隆体胞内染色，最后进行流式细胞仪分析。结果表明：初诊AML患者中CD4^＋和CD8^＋T淋巴细胞胞内IFNγ分泌水平均明显低于健康成人外周血T淋巴细胞，CD4^＋和CD8^＋T细胞胞内IL-4分泌水平与成人外周血细胞无显著差异。处于临床缓解状态的AML患者，CD8^＋T淋巴细胞刺激后胞内产生IFNγ的量明显高于初诊AML患者（P〈0、05）．但与健康成人无显著差异（P〉0、05）。复发的AML患者外周血中CD4^＋T细胞和CD8^＋T细胞刺激后胞内产生IFNγ量明显低于健康成人外周血T淋巴细胞以及处于完全缓解状态的AML患者CD8^＋T淋巴细胞（P〈0、05）．而IL-4的量明显高于健康成人和初诊AML患者CD4^＋T细胞和CD8^＋T细胞（p〈0．05）。结论：处于不同状态下的AML患者T细胞亚群分泌的细胞因子发生了改变，与之相应的是，初次诊断的AML患者外周血中CD4‘和CD8’T淋巴细胞刺激后Th1／Tcl细胞反应低下，Th2／Tc2细胞反应与健康成人T淋巴细胞无差异；完全缓解状态的AML患者T细胞Thl反应虽然仍低下，但Tcl反应明显增强，与健康成人无差异；复发的AML患者CD4^＋和CD8^＋T细胞Th2／Tc2样反应较Thl／Tcl样反应明显增强。     

Flow cytometric analysis of IL-6 receptors on peripheral lymphocytes in patients with primary biliary cirrhosis

Interleukin-6 receptors (IL-6R) and interleukin-1 receptors (IL-1R) on lymphocyte surfaces were analyzed, using flow cytometry and dye-labeled IL-6 and IL-1β, to examine the clinical and immunological significance of these receptors. Incubation of peripheral blood mononuclear cells in the presence of mitogen resulted in a remarkable increase of lymphocytes expressing the IL-6 and IL-1β receptors on the cell surface. The increase in lymphocytes bearing these cytokine receptors may reflect an increase in stimulated lymphocytes. When peripheral blood from patients with primary biliary cirrhosis (PBC) was examined for these receptors, the percentage of IL-6R positive cells was significantly higher in the patients than in healthy controls (P<0.01). The increase in IL-6R positive cells was only significant for the T lymphocyte fraction (P<0.01). No significant change in IL-1R was observed. There was a significant positive correlation between the percentage of IL-6R positive T lymphocytes and the titer of antimitochondrial antibody in patients with PBC. These findings concerning IL-6R may be noteworthy elucidating autoimmune etiological features of PBC. J. Clin. Lab. Anal. 12:83–87,1998. © 1998 Wiley-Liss, Inc.     

212例活动性结核病患者外周血淋巴细胞亚群特点:单中心描述性研究

  目的  基于相对较大的样本数据,探究活动性结核病(active tuberculosis, ATB)患者外周血淋巴细胞亚群特点及其意义。  方法  回顾性收集北京协和医院住院ATB患者(2012年6月—2022年1月)及年龄、性别与其相匹配的健康人群(2019年3月—2022年5月)的临床资料。以健康人群为对照,分析ATB患者外周血淋巴细胞亚群变化特点,并对不同确诊方式及受累部位ATB患者进行组间比较。  结果  共入选符合纳入与排除标准的ATB患者212例、健康人群200例。ATB患者中,临床确诊82例(38.7%)、病原学确诊130例(61.3%, 包括单纯肺结核77例、单纯肺外结核21例、肺结核并肺外结核32例)。相较于健康人群,ATB患者多种外周血淋巴细胞亚群指标计数及其百分比降低(P均＜0.05),T淋巴细胞百分比、CD8⁺T淋巴细胞百分比、记忆CD4⁺T淋巴细胞/CD4⁺T淋巴细胞比值、CD38⁺CD8⁺T淋巴细胞/CD8⁺T淋巴细胞比值等均升高(P均＜0.05)。相较于临床确诊ATB患者,病原学确诊ATB患者淋巴细胞、B细胞、自然杀伤(natural killer,NK)细胞、T淋巴细胞、CD4⁺T淋巴细胞、CD8⁺T淋巴细胞计数等均降低(P均＜0.05)。不同结核感染部位的患者记忆CD4⁺T淋巴细胞、纯真CD4⁺T淋巴细胞、CD28⁺T淋巴细胞及活化CD8⁺T淋巴细胞亚群计数及其百分比无显著差异(P均＞0.05)。相较于单纯肺结核患者,肺结核并肺外结核患者B细胞计数[23(10,69)个/μL比73(25,133)个/μL, P=0.003]降低,单纯肺外结核患者NK细胞百分比[16.7%(10.8%,23.6%)比10.6%(5.3%,17.4%), P=0.042]升高。  结论  ATB患者外周血淋巴细胞亚群指标整体呈减低状态,存在明显的免疫功能紊乱。     

Functions of T cells and recruitment of cellular response afterin vitro mitogenic stimulation of lymphocytes in chronic lymphocytic leukaemia

Summary In vitro stimulation of peripheral blood lymphocytes from ten patients with chronic lymphocytic leukaemia (CLL) was investigated under various culture conditions. It was confirmed that the mitogenic reactivity of whole cell populations (PBL) was delayed and depressed. When CLL rosette-forming cells (RFC) were stimulated, their³H-thymidine uptake was increased, but the pattern of the response was similar to that of whole PBL, thus suggesting some impairment of these cells. Furthermore, in order to evaluate the possible recruitment of B cells, generally thought to be unresponsive, some co-culture experiments were performed in which 10⁴ normal lymphocytes and 10⁵ CLL whole PBL or RFC-depleted cell populations were stimulated with mitogens. An amplified response of the CLL lymphocytes was obtained in all co-cultures, and this effect was more evident when specific T cell stimulants were used; autologous CLL T lymphocytes, on the contrary, failed to display such a ‘synergic’ effect. These results indicate that normal lymphocytes are able to recruit a large number of CLL lymphocytes in the mitogenic response; furthermore, the fact that in co-cultures of CLL T-depleted fractions a better response was obtained with T cell mitogens suggests that the definition of CLL as a clonal expansion of unresponsive ‘B’ lymphocytes may be inadequate.