Editorial Board of Journal of Medical Colleges of PLA

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Editorial Board of Journal of Medical Colleges of PLA

EDYr0R IN CPll又F 研几、NG Zheng一gu。(王正国)YAO Kai一tai(姚开泰)zHoU shu一石a(周树夏)ZH-U cheng(朱诚) ASSOCIATE EDITOR】N CBIEF HuANG uang一tian(黄良田)sHEN zhi一chao(沈志超)W飞气NG zlleng一ai(王征爱)zHANG Da一chlin(张大春) C()入i爪4ITI王厄MAN BIAN xiu一wu(卞修武)JIANGJi皿城n(蒋建新)xuFu一而ng(许福明) cAI服n一中n(蔡文琴)JL气NG Yuan一厂ng(姜远英)Xu Gang(徐刚) CAO Tie一sheng(曹铁生)JINBo一quan(金伯泉)XULi一xian(徐礼鲜) cHE xiao一yan(车小燕)JU Gong(鞠躬)Xu…     

Treatment of A Case of Spontaneous Rupture of Achille''''s Tendon

Mr.Guo,51 years old,paid his first visit onNovember 7,2001.The patient felt sudden pain inthe right Achille's tendon in June 1999 with noapparent predisposing factors.Several months laterhe palpated a small pitting in the middle of the rightAchille's tendon,which expanded gradually in size.In June 2000,two thirds of the tendon was diagnosedto be ruptured in a local hospital.Ten days later thetendon was ruptured completely when he felt suddensevere pain,numbness and distention,and he wasunable to raise the heel.The lesion was healed three     

Fast estimation of Michaelis-Menten constant of arylesterase with a pair of medi-um concentrations of substrate

Objective: To investigate the reliability for fast estimation of Michaelis-Menten constant (Km) with calibra-ted specific activity at only two medium concentrations of substrate by both simulation and experimentation with aryles-terase (ArE)as model. Methods: Initial rates were simulated by randomly inserting uniform absolute error, and the experimental initial rates of ArE were determined by measuring the increaser of product absorbance. Calibrated specific activities at two substrate concentrations were obtained by regression analysis, and Km was calculated according to Michaelis-Menten equation. Results: By simulation with calibrated specific activities at two medium substrate concen-trations, Km could be calculated according to Michaelis-Menten equation with reasonable precision and accuracy. By experimentation with substrates of 2-naphthyl acetate, phenyl acetate, and p-nitrophenyl acetate, there were no differ-ences between the mean and SD of Km of ArE for either substrate by this linear kinetic method and the Lineweaver-Burk plot. Conclusion: This linear kinetic method was reliable for fast estimation of the Km of some specified enzyme on its substrate of lower solubility or lower sensitivity for quantification by common methods.     

Journal of Medical Colleges of PLA

EDITOR IN CHIEFWANG zheng一邵。(王正国)YAo Kai一tai(姚开泰)zHOU Shu一xia(周树夏)ZHU Cheng(朱诚)ASSOCIATE EDITOR IN CHIEFHUANG Liang一tian(黄良田)SHEN zhi一ehao(沈智超)WANG zheng一ai(王征爱)zHANG Da一ehun(张大春)EDITORLAL BOARDBIAN xiu一wu(卞修武)JIANG Jian一xin(蒋建新)XU Fu一ming(许福明)CAI Wen一qin(蔡文琴)JIANG Yuan一ying(姜远英)XU Gang(徐刚)CAO Tie一sheng(曹铁生)JIN Bo一quan(金伯泉)Xu Li一xian(徐礼鲜)CHE Xiao一yan(车小燕)JU Gong(鞠躬)XU Ru一x…     

Effects of Spinach Powder Fat-Soluble Extract on Proliferation of Human Gastric Adenocarcinoma Cells

INTRODUCTIONMoreandmoreattentionhasbeenfocusedontheanti-cancereffectofvegetablesinre-centyears.Inpreviousepidemiologcalstudies,ithasbeenconcludedthatahighlevelofconsumptionofvegetablesandfruitsisconsistentlyassociatedwithareducedriskofepithe-lialcancers,particulariythoseofthealimentaryandrespiratorytracts(SteinmetsandPot-ter,l99l).Meanwhile,manyresearchershavestudiedthepossiblemechanismsforthean-ti-cancereffectsofvegetablesandfruits(SteinmetsandPotter,l992).Ininvitrostudies,someofthefat-…     

Effect of Acanthopanax giraldii Harms Var.Hispidus Hoo polysaccharides on the human gastric cancer cell line SGC-7901 and its possible mechanism

Objective To study the inhibitory effect of Acanthopanax giraldii Harms Var.Hispidus Hoo polysaccharides (AGP) onSGC-7901 gastric cancer cells and its possible mechanism. Methods Cell doubling time analysis, colony forming assay and MTT assay were adopted to study the inhibitory effect and its characteristics. We also analyzed the amount of protein expressed by oncogenes, antioncogenes and cell factors using flow cytometric analysis.Results AGP inhibited the proliferation of SGC-7901 cells and cell colony forming ability. AGP did not inhibit the viability and function of lymphocytes of peripheral blood in healthy subjects and human embryonic tenocytes, except for the highest dosage of AGP ( P <0. 05), which slightly inhibited the viability and function of the two types of normal cells. AGP inhibited the viability and function of SGC-7901 cells, except for the lowest dosages of AGPⅠ and AGPⅢ. There was a dose-effect relationship between the dosage of the AGP andSGC-7901 cells.The effect of the AGP at the molecular level was associated with the low protein expression of the c-myc and bcl-2 genes and the high protein expression of the p53, bax, fas and fas-L genes, as well as the cell factor TGFβ(1).The inhibitory effect of AGP was weaker than that of CDDP, but was stronger than that ofVitamine C. Conclusions Acanthopanax giraldii Harms Var.Hispidus Hoopolysaccharides selectively inhibited the proliferation, the colony forming ability, and the viability and function of human gastric cancer cells through the low protein expression of c-myc, bcl-2 and the high protein expression of p53, fas, fas-L and the cell factor TGFβ(1). The different inhibitory characteristics on the normal cells and cancer cells are possibly caused by gene and the cell factor expressions.     

大蒜、芦笋对人胃癌细胞SGC-7901增殖的影响

观察大蒜汁、新鲜芦笋汁对体外培养的胃癌细胞SGC-7901的增殖抑制作用,探讨其可能的作用机制.传代培养胃癌细胞SGC-7901,采用MTT法检测大蒜汁、新鲜芦笋汁作用后细胞的增殖抑制率.结果:浓度从10-50 μl/ml的大蒜汁和新鲜芦笋汁对SGC-7901细胞都有抑制增生作用,并随着药物浓度的升高,作用时间的延长,对SGC-7901细胞的抑制率也升高.结论:大蒜汁、新鲜芦笋汁能明显抑制胃癌细胞SGC-7901的增殖.     

香菇多糖的体外抗肿瘤活性研究

目的:研究香菇多糖体外抗肿瘤活性.方法:MTY法观察香菇多糖对胃癌细胞株SGC-7901增殖的影响,流式细胞术分析其对SGC-7901细胞周期的影响,Western blot法检测其对ERK1/2蛋白激酶磷酸化的作用.结果:香菇多糖能够显著抑制SGC-7901胃癌细胞的生长.香菇多糖作用于SGC-7901胃癌细胞后能够...     

1,25-二羟维生素D3对胃癌细胞增殖和TNF-α表达的影响

目的研究1,25-二羟维生素D3[1,25(OH)2D3]对胃癌SGC-7901细胞增殖和细胞周期的影响,以及对细胞因子TNF-αmRNA及蛋白表达的影响。方法用不同浓度1,25(OH)2D3处理胃癌SGC-7901细胞,采用MTT法检测细胞增殖情况;流式细胞仪检测细胞生长周期;RT-PCR和Western blotting分别检测TNF-α在mRNA和蛋白水平的表达情况。结果 1,25(OH)2D3对胃癌SGC-7901细胞增殖有抑制作用(P<0.05),并呈时间剂量依赖性。经1,25(OH)2D3处理72 h后细胞周期出现向G0/G1期移行的动力学改变(F=9.81,P<0.05)。TNF-αmRNA及蛋白的表达水平均呈剂量依赖性减少(P<0.05)。结论 1,25(OH)2D3可抑制胃癌细胞增殖,且与其抑制TNF-α的表达可能有相关性。     

阿司匹林抑制胃癌细胞生长及其机制探讨

目的　研究阿司匹林对 SGC- 790 1胃癌细胞生长的影响 ,并初步探讨其作用的分子机制。方法　采用 3H- Td R法及流式细胞术检测不同浓度阿司匹林对胃癌细胞 DNA合成及细胞周期的影响 ;用免疫细胞化学法检测阿司匹林对胃癌细胞 COX- 2表达的影响 ;Western blotting法及 EMSA法检测阿司匹林对胃癌细胞 c- fos表达及 AP- 1活化的影响。结果　胃癌细胞经不同浓度的阿司匹林作用后 ,其 3H- Td R掺入值明显降低 ,且与阿司匹林浓度呈高度负相关 ( r=- 0 .9,P<0 .0 5 )。细胞周期分析显示 ,阿司匹林主要作用于胃癌细胞 S期。免疫细胞化学染色显示 ,阿司匹林能下调胃癌细胞 COX- 2表达 ,且具有良好的量效关系。Western blotting检测表明 ,阿司匹林能降低胃癌细胞 c- fos蛋白的表达。EMSA分析显示 ,阿司匹林能有效抑制血清刺激的 AP- 1的 DNA结合活性。结论　阿司匹林能有效抑制胃癌细胞的生长 ,这种作用可能是其通过抑制胃癌细胞 c- fos表达以及 AP- 1活化 ,进而抑制COX- 2表达所致     

Effects of Serum Containing Chinese Medicine Sanpi Pingwei (散癖平胃) Formula on Proliferation and Apoptosis of Human SGC-7901 Cells

Objective:To investigate the effects of serum containing Chinese medicine(CM) Sanpi Pingwei(散癖平胃,SPPW) formula on the proliferation and apoptosis of human SGC-7901 cells and the possible mechanism.Methods:Serum containing CM SPPW formula(SPPW serum) was prepared by a serum pharmacology method.Human SGC-7901 cells were incubated with SPPW serum at three different concentrations and with the anticancer drug 5-fluorouracil(5-FU),respectively.Cell proliferation was assessed by MTT assay,and cell apoptosis was detected by flow cytometry assay.Real-time quantitative polymerase chain reaction(RT-PCR) and Western blot assay were employed to confirm the expressions of Bcl-2,Bax and p53 in SGC-7901 cells at mRNA and protein levels,respectively.Results:SPPW serum suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner.The colony forming rate of negative control was 48.2%,while those in the three SPPW serum groups and the 5-FU group decreased significantly (P<0.01).The number of colony forming units in the SPPW high dosage group was significantly smaller than that in the 5-FU group(P<0.01).MTT assay showed that SPPW serum restrained the proliferation of SGC-7901 cells,and the inhibition rate increased significantly in a dose-dependent manner.Annexin V/PI Assay suggested that SPPW serum induced the apoptosis of SGC-7901 cells significantly.RT-PCR and western blot assay indicated that SPPW serum upregulated the protein and mRNA expression levels of Bax and p53 in SGC-7901 cells,but downregulated the protein and mRNA expressions of Bcl-2.Conclusions:SPPW formula inhibits the proliferation of SGC-7901 cells in vitro and induces the cell apoptosis.It plays an anticancer role by regulating the expressions of Bax,p53 and Bcl-2 in SGC-7901 cells.     

白杨素增强rhsTRAIL对人胃癌SGC-7901细胞毒性

目的:研究白杨素(ChR)是否具有增强rhsTRAIL对人胃癌SGC-7901细胞毒性作用。方法:体外培养SGC-7901细胞。MTT比色法测定细胞毒性。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞死亡率。结果:ChR、rhsTRAIL以及两者合用对SGC-7901细胞活性的半数抑制浓度(IC50值)分别为134μmol/L、402ng/mL和47ng/mL,合并用药效应的CI值是0.4676。ChR40μmol/L、rhsTRAIL100ng/mL以及两者合用的细胞死亡率分别为4.65%±0.58%、3.60%±0.16%和49.87%±4.27%。结论:亚细胞毒性浓度的白杨素具有增强rhsTRAIL对人胃癌SGC-7901细胞毒性作用。     

四碘甲腺乙酸对耐药性人胃癌细胞系体内和体外增殖的抑制作用

目的：研究四碘甲腺乙酸(Tetrac)对阿霉素(Dox)抵抗性人胃癌细胞系SGC-7901/R体内及体外扩增的抑制作用及作用机制。方法：体外实验分为SGC-7901与SGC-7901/R两组,分别与不同浓度的Tetrac和Dox共同培养4 d。MTT法检测其细胞活性,以不同药物浓度下的肿瘤细胞存活率来表示;Western blotting检测2组中相关耐药基因P-gp、SOD和GST的表达。分别将Dox、依拉泊苷(Etop)、顺铂(Cisp)3种药物单独或联合Tetrac作用于SGC-7901/R细胞系,用MTT法、衰老相关的β-半乳糖苷酶(SA-b-Gal）染色法与凋亡相关的烟酸已可碱（Hoechst）染色法检测肿瘤细胞活性、衰老及凋亡情况,检测结果以染色阳性细胞比例表示。体内实验中,腋部皮下注射SGC-7901/R细胞（10⁶ /100 μL）的裸鼠被分成4组（每组7只）：生理盐水对照组、Tetrac治疗组（30 mg/kg）、Dox治疗组（2 mg/kg）和Tetrac（30 mg/kg）+Dox（2 mg/kg）联合药物治疗组,检测各组小鼠肿瘤生长情况。结果：体外实验中,2组肿瘤细胞均随Tetrac浓度的升高而坏死明显加快,在100 mg/L时全部死亡;P-gp转运子仅在SGC-7901/R细胞中过表达;Dox、Etop、Cisp与 Tetrac联合用药时,SGC-7901/R细胞存活率较单独用药明显降低(P<0.001);Dox与Tetrac联合用药时,SGC-7901/R细胞衰老、死亡较单独用药明显增加(P<0.05)。体内实验中,Dox与Tetrac联合用药可以明显抑制肿瘤生长。结论：Tetrac可能通过降低药物转运子P-gp表达来促进SGC-7901/R细胞的衰老和凋亡,对于治疗Dox抵抗性肿瘤是一种有效的化疗药物。     

17肽胃泌素及其受体拮抗剂L-365260对人胃癌细胞株SGC-7901生长的影响

目的 旨在探讨17肽胃泌素(G-17)及L-365260对人胃癌细胞生长的影响及其作用机制。方法 采用G-17及L-365260作用于人胃癌细胞株SGC-7901,通过MTT活细胞染色法检测细胞增殖抑制率,流式细胞仪检测Bcl-2和Bax蛋白表达。结果 G-17在1×10-9～1×10-5mol/L浓度范围内对SGC-7901细胞有明显促进作用。L-365260在1×10-8～1×10-5mol/L浓度范围内对SGC-7901细胞有明显抑制作用。L-365260可明显抑制G-17对SGC-7901细胞的促生长作用。G-17可使SGC-7901细胞中的Bcl-2蛋白表达显著增加,对Bax蛋白无影响。L-365260不仅可使SGC-7901细胞中的Bcl-2蛋白表达显著减少,而且还可使Bax蛋白表达显著增加。结论 G-17对人胃癌细胞生长具有促进作用,而L-365260则对其具有抑制作用。上调Bcl-2蛋白表达,以抑制SGC-7901细胞凋亡可能是G-17促进胃癌细胞生长的机制之一。L-365260可能通过下调Bcl-2、上调Bax蛋白表达,诱导SGC-7901细胞凋亡,以抑制癌细胞生长。