自制镍钛记忆合金网状内支架气管内生物相容性实验研究

目的：研究气管内镍钛金属网状内支架生物相容性。方法：在实验兔气管内放置３０枚网状内支架，通过观察其１周～６个月局部组织和全身反应，初步评价其生物相容性。结果：自制镍钛记忆内支架具有局部组织反应较轻，局部管腔通畅，全身重要脏器无改变，植入后记忆合金无腐蚀生锈。结论：自制镍钛记忆合金具有良好的力学性能和生物相容性，是一种理想的生物医用材料。     

TGF-β1在羟基磷灰石多孔层修饰镍钛合金修复骨缺损中的表达及意义

目的：研究TGF-β1在羟基磷灰石多孔层修饰镍钛合金修复骨缺损过程中的表达和意义,为羟基磷灰石多孔层修饰镍钛合金作为生物医学材料在临床上广泛应用提供理论依据。方法：选取健康成年兔24只,随机分为实验组和对照组,选取左侧股骨下1／3处为种植区,其中实验组植入羟基磷灰石多孔层修饰镍钛合金,对照组植入镍钛合金,通过大体观察、HE染色观察术后1,2,4,8wk骨组织局部组织反应、骨缺损的修复情况以及免疫组织化学染色测定TGF-β1的表达。结果：大体观察、常规HE染色显示实验组1,2,4,8wk骨痂生长、骨缺损修复情况明显优于对照组;免疫组织化学染色显示实验组于1,2,4,8wk TGF-β1表达明显高于对照组,且具有统计学差异（P〈0.05）。结论：羟基磷灰石多孔层修饰镍钛合金能够刺激TGF-β1的表达,促进骨缺损修复;羟基磷灰石多孔层修饰镍钛合金与骨组织具有很好的相容性。     

自制镍钛记忆合金网状内支架气管内生物相容性实验研究

目的 :研究气管内镍钛金属网状内支架生物相容性。方法 :在实验兔气管内放置 30枚网状内支架 ,通过观察其 1周～ 6个月局部组织和全身反应 ,初步评价其生物相容性。结果 :自制镍钛记忆内支架具有局部组织反应较轻 ,局部管腔通肠 ,全身重要脏器无改变 ,植入后记忆合金无腐蚀生锈。结论 :自制镍钛记忆合金具有良好的力学性能和生物相容性 ,是一种理想的生物医用材料     

镍钛形状记忆合金骨内植入生物安全度的深入研究

目的：观察表面处理和时间因素对镍钛形状记忆合金骨内植入后犬重要器官及组织中镍含量的影响。方法：将镍钛记忆合金牙科种植体植入犬股骨内,以表面是否复合氧化铝生物陶瓷分为复合组织和镍钛组,另设对照组,分别测定犬肝,肾,脾及鼻咽粘膜中镍的含量,并作组织病理学观察。结果;镍钛组镍含量有随时间增高的趋势,但各器官组织有所不同,复合组镍含量明显低于镍钛组６个月时,但仍高于对照组;两组组织病理学观察均未见明显异常     

钛镍记忆合金皮内缝合的临床研究

目的 观察用钛镍记忆合金组织吻合器进行手术切口皮内缝合的临床疗效.方法 总结我院2010年剖宫产术中的120例产妇,60例采用钛镍记忆合金组织吻合器皮内缝合切口皮肤(实验组),60例采用普通丝线间断缝合切口皮肤(对照组),比较两组切口拆线时间和皮肤愈合情况.结果 实验组优于对照组.结论 我们认为钛镍记忆合金组织吻合器是一种良好、实用的新型缝合器材,值得临床推广使用.     

镍钛记忆合金材料在骨科的应用

目的探究镍钛记忆合金材料在骨科的应用现状。方法通过对镍钛记忆合金在骨科的具体使用方法和类型进行研究,评价镍钛记忆合金材料骨科应用的价值。结果在骨科镍钛记忆合金材料可应用于四肢、脊柱及股骨头的骨折中,临床应用效果较为满意。结论镍钛记忆合金材料拥有较好的生物相容性,其较好的弹性及不易磨损、抗腐蚀的优点,在骨科骨折的治疗上得到了广泛的应用。     

3种支架对喉气管狭窄疗效的比较分析

目的:比较3种支架在喉气管狭窄成形术的疗效。方法:对35例接受3种支架植入术在喉气管狭窄成形手术治疗中的疗效进行回顾性分析。结果:镍钛记忆合金支架(NT-SMA)组织相容性好,拔管率明显高于自凝塑料和医用硅胶T型支架(P<0.01)。结论:喉气管狭窄成形术中植入镍钛记忆合金支架可明显提高术后的拔管率。     

镍钛记忆合金气管内植入的基础研究

镍钛记忆合金是一种优良的生物材料，但在腔道内应用的基础研究较少。本文报告了该合金植入气管内６个月的基础研究，结果表明：对局部组织无损害，炎症反应轻，合金丝逐渐被新生的纤维包膜包绕和新生柱状上皮覆盖。未见变色，腐蚀及细胞毒性现象。具有优良的生物相容性和耐腐蚀性能，是一种较理想的腔道内植入材料。     

镍钛记忆合金支架治疗气管狭窄4例报告

目的：探讨镍钛记忆合金腔内支架在气管狭窄中的应用。方法：为1例气管下段断裂并狭窄和3例晚期肿瘤所致之重度气管狭窄的病人植入记忆合金支架，观察治疗前后病人的呼吸情况及远期疗效。结果：支架长期放在肉芽、瘢痕化的气管狭窄部位，未见引起严重的炎症反应或刺激症状，无分泌物及肉芽组织增生造成阻塞的情况发生，支架表面光洁，无移位及变形。结论：采用镍钛记忆合金腔内支架治疗气管狭窄能明显改善病人生活质量，疗效显著。     

镍钛记忆合金环抱器治疗多发性肋骨骨折的临床研究

目的：探讨镍钛记忆合金环抱器治疗多发性肋骨骨折的疗效。方法100例多发性肋骨骨折患者均采用镍钛记忆合金环抱器行手术治疗。结果患者均无感染,骨折不愈合及内固定松动、滑脱等并发症发生;术后5个月~12个月取出内固定,骨折部位无炎症反应,心肺功能恢复较好。结论镍钛记忆合金环抱器呈现出良好的组织相容性,手术操作简便、创伤小、并发症少,是一种较为理想的治疗多发性肋骨骨折的方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.