慢性乙型肝炎外周血T淋巴细胞亚群变化及其临床意义

目的:比较慢性乙型肝炎(CHB)感染者外周血T细胞亚群的变化,探讨其与临床疾病状态的关系。方法:用流式细胞术(FCM)检测HBeAg阳性ALT异常者42例(A组);HBeAg阳性ALT持续正常者25例(B组);HBsAg阳性、HBeAg、HBV-DNA及ALT正常者20例(C组);健康体检(各型病毒学指标均为阴性)者25例外周血中T淋巴细胞亚群。结果:A、B组CD4+T细胞数量较C组及正常组明显降低;而CD8+T细胞数量较正常组明显升高(P0.01),CD4+/CD8+、CD4+CD25+T细胞与正常组比较明显降低(P0.05)。结论:慢性乙肝HBeAg阳性ALT正常患者Ts细胞数量增多,Th细胞和CTL细胞受到抑制,乙肝病毒复制与CD4+T细胞及CTL数量低下,抑制性T细胞增高高度相关。通过T淋巴细胞亚群的检测可以监控患者的免疫状态,从而为指导临床免疫治疗提供依据。     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

目的 探讨辅助性T淋巴细胞(Th细胞)和细胞毒性T淋巴细胞(Tc细胞)的极化情况在尖锐湿疣(CA)发病机理中的作用及其与疾病复发的关系.方法 采用三色荧光抗体染色流式细胞术检测细胞内细胞因子的方法 ,检测30例CA患者和20名健康对照者外周血CD3+CD8-/IFN-γ+(Th1)、CD3+CD8-/IL4+(Th2)、CD3+CD8+/IFN-γ+(Tc1)和CD3+CD8+,IL-4+(Tc2)细胞比例.结果 与健康对照组比较,CA患者外周血中Thl细胞含量显著减少(P<0.01),Tc1细胞含量、Th1/Th2比值、Tc1/Tc2比值均减少(P<0.05);其中15例复发CA患者的Th1/Th2比值较正常对照显著减少(P<0.01),而Te1/Te2比值与健康对照组比较差异无统计学意义.结论 CA患者外周血Th1和Tc1细胞数减少,而Th2和Tc2细胞相对占优势,复发的CA患者这种失衡更趋严重.CA患者机体免疫状态可能存在Th1→Th2、Tc1→Tc2方向的漂移,这种漂移可能是人乳头瘤病毒(HPV)不易被有效清除或CA反复发生的一种免疫机制.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

肺癌患者外周血T淋巴细胞亚群变化特点及临床意义

目的:探讨肺癌患者外周血T淋巴细胞变化及其临床意义。方法:采用流式细胞术检测法检测56例肺癌患者(其中20例早中期肺癌、36例晚期肺癌);18例健康人群外周血单个核细胞(Peripheral blood mononuclear cells,PBMC)中总T细胞(CD3+)、Th细胞(CD3+CD4+)、Tc细胞(CD3+CD8+)、NKT细胞(CD3+CD16+CD56+)、NK细胞(CD3-CD16+CD56+)、调节性T细胞(Treg,CD4+CD25+Foxp3+)占CD4+T细胞比例和CD3+γδT细胞比例。结果:56例肺癌患者总T细胞(CD3+)比例明显低于健康组(61.41%±7.88%vs71.63%±5.59%,P0.001),肺癌患者Tc细胞比例明显低于健康组(23.58%±7.18%vs28.44%±5.20%,P0.05),肺癌患者Treg比例明显高于健康组(6.20%±1.63%vs3.65%±2.00%,P0.001);肿瘤组外周血CD3+γδT细胞比例显著低于健康组(3.35%±1.41%vs5.53%±1.87%,P0.01)。但肺癌患者晚期组与肺癌早中期组比较外周血CD3+γδT细胞比例显著增高(3.70%±1.89%vs2.64%±1.41%,P0.05),肺癌患者晚期组与早中期组比较外周血Treg细胞比较显著增高(6.78%±2.64%vs5.06%±1.22%,P0.05)。肺癌患者外周血中NK细胞低于健康组(15.02%±7.61%vs18.74%±6.39%,P0.05),而Th细胞和NKT细胞都有所降低,但差异尚不具统计学意义(P0.05)。结论:肺癌患者总T细胞、Th细胞、Tc细胞和NK细胞都有所降低,Treg细胞有所上升,提示肺癌患者处于免疫抑制状态。Treg细胞和CD3+γδT细胞与肺癌的临床病程具有一定的关联性。     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

病毒性肝炎患者外周血T细胞亚群的变化

<正> 应用APAAP桥联酶标法,检测50例急、慢性病毒性肝炎患者在不同病期时,其外周血T淋巴细胞亚群的变化,结果如下。 材料和方法 一、病例:急性黄疸型肝炎30例,其中甲型和乙型肝炎各半。患者男性21例,女性9例,平均年龄28.7±8.8岁,总胆红质(TBil)71.74±41.31μmol/L,谷丙转氨酶(ALT)均在150u以上(正常<40u)。慢性无黄疸型乙型肝炎20例,其中慢性迁延性肝炎12例,慢性活动性肝炎8例,ALT均在150u以上,男16例,女4例,平均年龄30.1±6.4岁。同时检测10例健康人T细胞亚群,以资对比。     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.     

尖锐湿疣患者外周血Thl/Th2及Tc1/Tc2亚群的检测及意义

Objective To investigate the role of T-helper (Th) and cytotoxic T (Tc) lymphocyte polarization in the pathogenesis of condyloma acuminatum (CA) and its correlation with recurrence. Methods Three-colour immunofluorescent flow cytometry was used to detect the proportion of CD3+ CD8-/IFN-γ+ (Th1),CD3+CD8-/IL-4+ (Th2), CD3+ CD8+/IFN-γ+ (Tel) and CD3+ CD8+/IL-4+ (Tc2) cells in the peripheral blood of CA patients and health controls. Results Compared to health controls, CA patients showed a decreased number of Thl (P < 0.01) and Tcl cells (P < 0.05), as well as a decreased Th1/Th2 and Tc1/Tc2 ratio (P <0.05). Furthermore, in 15 recurrent CA patients the ratio of Th1/Th2 was remarkably decreased (P < 0.01),while the ratio of Tc1/Tc2 had no significant change in comparison with health controls. Conclusion The decrease of Th1 and Tc1 subsets results in relative Th2 and Tc2 predominance, and this tendency is more significant in recurrent CA patients. The Th1 to Th2 and Te1 to Tc2 shifts in CA patients could be responsible for the fact that human papilloma virus (HPV) is hard to be eliminated.