消肿止痛膏的抗炎镇痛作用

目的 研究消肿7止痛膏外涂给药的抗炎镇痛作用。方法 采用小鼠巴豆油性耳肿、大鼠角叉菜胶足肿、大鼠佐剂性关节炎等模型观察了消肿止痛膏的抗炎作用,并采用小鼠温热刺激、化学刺激、电刺激和大鼠温热刺激等方法观察消肿止痛膏的镇痛作用。结果 在0．25－4．00g生药／kg剂量对上述3种实验性炎症均有非常显著的抑制作用,在1．0－10．0g生药／kg剂量对上述3种刺激也非常显著的镇痛作用。结论 消肿止痛膏外涂给药具有非常显著的抗炎镇痛作用。     

新癀片中药组分对吲哚美辛的减毒作用

目的 探讨新癀片中的中药组分对化学药成分吲哚美辛的减毒作用。方法 采用急性毒性实验。给ICR小鼠分别ig不同剂量的新癀片、化学药成分吲哚美辛,记录各组小鼠的死亡数、毒性症状,用Bliss法分别计算LD₅₀。同时另设新癀片中药组分组,确定其最大给药量,并观察毒性反应。结果 新癀片中药组分对小鼠的1日最大给药量为18.2 g/kg。吲哚美辛的LD₅₀为18.31 mg/kg,新癀片的LD₅₀为1 217.83 mg/kg,由于吲哚美辛在新癀片中的质量分数为2.478%,因此折算成新癀片中吲哚美辛的LD₅₀为30.16 mg/kg。结论 新癀片中中药组分对吲哚美辛具有减毒作用。     

翁沥通胶囊中铜绿的拆方研究

目的 研究铜绿在翁沥通胶囊镇痛、抗炎及治疗前列腺增生功效中的作用。方法 采用醋酸扭体模型比较不含铜绿的翁沥通胶囊 (A 药) 和含铜绿的翁沥通胶囊 (B 药) 的镇痛作用;采用小鼠二甲苯炎症模型比较 A 药和 B 药的抗炎作用;采用小鼠 sc 丙酸睾酮诱导前列腺增生模型,比较 A 药和 B 药的抗前列腺增生作用。结果 连续 ig 7 d 翁沥通 A 药和 B 药 0.6、1.2、2.4、4.8 g/kg (按生药量计算),小鼠 15 min 内的扭体次数均显著少于对照组 (P＜0.05、0.01)。翁沥通 B 药 1.2、2.4、4.8 g/kg 及翁沥通 A 药 2.4 g/kg 连续 ig 7 d,明显减轻小鼠耳片肿胀度 (P＜0.05、0.01);翁沥通 A 药的其他4个剂量组的小鼠耳片肿胀度与对照组相比均无明显差异。翁沥通 B 药 0.3、1.2 g/kg 连续 ig 14 d,明显减轻前列腺增生模型小鼠的前列腺湿质量及湿质量系数、前列腺干质量及干质量系数 (P＜0.05、0.01);翁沥通 A 药仅有 1.2 g/kg 明显减轻模型小鼠的前列腺湿质量、湿质量系数及前列腺干质量 (P＜0.05、0.01),对前列腺干质量系数无明显影响 (P＞0.05)。结论 铜绿在翁沥通胶囊的组方中,对于该方剂的抗炎和抗前列腺增生功效发挥重要作用。     

白桂木抗炎镇痛作用有效部位筛选

目的 分离并筛选白桂木抗类风湿性关节炎的有效部位。方法 通过连续 ig 给药白桂木根总提取物、石油醚部位、醋酸乙酯部位、氯仿部位提取物,分别采用小鼠二甲苯致耳肿胀、大鼠棉球所致肉芽肿、小鼠醋酸扭体法、小鼠热板法和小鼠腹腔毛细血管通透性评价活性。结果 白桂木醋酸乙酯部位抗炎镇痛作用较明显。结论 白桂木醋酸乙酯部位可能是其治疗类风湿性关节炎的有效部位。     

痹祺胶囊全方及拆方抗炎镇痛作用研究

目的 研究痹祺胶囊全方及拆方的镇痛、抗炎作用,探讨君、臣、佐、使间的配伍规律。方法 采用小鼠热板法和扭体法观察痹祺胶囊及拆方的镇痛作用;采用大鼠佐剂关节炎 (AA) 模型观察痹祺胶囊及拆方的抗炎作用。结果 君药马钱子 ig 给予小鼠 50 mg/kg (相当于处方用量),大鼠 2.5 mg/kg (相当于处方用量),其镇痛、消肿作用并不明显,只有高于处方2倍以上的用量,方可起到一定镇痛作用;动物单独 ig 臣、佐、使药后其镇痛、消肿作用均不明显;君+臣、君+佐、君+臣+使、君+佐+使药配伍后镇痛作用不明显,对大鼠 AA 原发性和继发性肿胀有明显消肿作用;君+使药配伍的镇痛作用和对大鼠 AA 原发性肿胀的消肿作用明显,而对 AA 大鼠足趾继发性肿胀的消肿作用不明显;君+臣+佐药配伍既有明显镇痛作用,又有明显的消肿作用,痹祺胶囊全方 (君+臣+佐+使) 无论对小鼠的镇痛作用还是对 AA 大鼠足趾肿胀的消肿作用均非常明显。结论 痹祺胶囊具有明显的抗炎、镇痛作用,臣、佐、使药对君药马钱子有减低用量,增强疗效的作用。     

镰形棘豆提取物经皮给药的抗炎镇痛活性

目的 研究镰形棘豆总黄酮与挥发油组合物（FEO）经皮给药的抗炎镇痛活性。方法 用二甲苯诱导的小鼠耳肿胀模型和大鼠佐剂性关节炎模型研究FEO的抗炎活性,采用热板法和醋酸扭体法研究FEO对小鼠的镇痛活性。FEO对小鼠的给药剂量分别为生药3.120、0.390、0.195 g/kg;对大鼠的给药剂量分别为生药2.160、0.270、0.135 g/kg。以扶他林为阳性对照药。结果 FEO高剂量经皮给药对小鼠耳肿胀的抑制率达66.03%;使大鼠佐剂性关节炎的早期肿胀和继发性肿胀明显减轻至35.79%、3.27%。在热板法实验中,FEO高剂量给药30、60、90 min后对热板致小鼠疼痛的抑制率分别为42.37%、47.49%、72.00%;对醋酸扭体法引起的疼痛也有一定镇痛作用,抑制率为56.67%。结论 FEO高剂量经皮给药方式有良好的抗炎镇痛活性。     

舒督丹抗炎镇痛作用的研究

按照处方的功能主治，采用4种炎症模型和2种镇痛模型，研究了舒督丹的抗炎镇痛作用。结果发现10、20、40g生药／kgig给药，对小鼠腹腔毛细血管通透性和巴豆油性耳肿的抑制作用非常显著，对大鼠角叉菜胶足肿和佐剂关节炎的抗炎作用也非常显著。既能提高大鼠和小鼠对温热刺激的痛阈值，又能减少小鼠对化学刺激的扭体次数，其镇痛作用具有起效快和作用时间长的特点。为该药在临床上治疗强直性脊柱炎、类风湿关节炎等提供了实验依据。     

1-甲氧基钩吻碱抗慢性疼痛作用

目的 1-甲氧基钩吻碱对福尔马林足底注射所致炎性痛及小鼠坐骨神经慢性压榨性损伤 (CCI) 所致神经病理性疼痛的作用。方法 建立小鼠福尔马林炎性疼痛模型,以注射福尔马林后 1 h 内小鼠舔足时间为指标,观察 1-甲氧基钩吻碱对炎性痛第二相痛反应的影响;建立小鼠 CCI 模型,于术后第3天至术后第9天 sc 1-甲氧基钩吻碱,每天2次,连续 7 d,以双足热痛缩足潜伏期比值为指标,观察 1-甲氧基钩吻碱对小鼠足底热痛缩足潜伏期的影响。结果 1-甲氧基钩吻碱具有显著对抗小鼠福尔马林致炎性疼痛第二相痛反应作用,作用呈剂量依赖性,ED₅₀ 为 4.59 mg/kg。1-甲氧基钩吻碱具有显著的抗 CCI 小鼠热辐射痛敏的作用,镇痛效应呈剂量依赖性;高剂量组小鼠给药后 2 d 显效,镇痛疗效持续至停药后 36 h;术后第9天,1-甲氧基钩吻碱镇痛效应 ED₅₀ 为 3.84 mg/kg。结论 1-甲氧基钩吻碱具有抗炎性疼痛作用与抗神经病理性疼痛作用。     

小儿抗病毒颗粒药效学研究

目的 观察小儿抗病毒颗粒的解热祛痰、镇咳平喘作用。方法 采用干酵母致热造模法观察小儿抗病毒颗粒对大鼠的解热作用,给药剂量分别为4.18、2.09、1.05 g/kg,每天ig给药1次,连续3 d,末次给药后sc干酵母混合溶液,4～8 h后测大鼠体温。采用酚红比色法观察其对小鼠的祛痰作用,给药剂量分别为5.22、2.61、1.31 g /kg,每天ig给药2次,连续7 d,末次给药1 h后进行检测。采用氨水吸入引咳法观察其对小鼠的镇咳作用,给药剂量同祛痰实验,每天ig给药2次,连续3 d,观察潜伏期和咳嗽次数。采用雾化乙酰胆碱吸入引喘法进行豚鼠平喘试验,给药剂量分别为3.65、1.83、0.914 g /kg,每天给药2次,连续3 d,测定引喘潜伏期。结果 小儿抗病毒颗粒能使小鼠气管酚红的分泌量增加（P＜0.05）;显著抑制由干酵母引发的大鼠发热反应（P＜0.05）及小鼠的咳嗽潜伏期延长（P＜0.05）;使豚鼠哮喘潜伏期显著延长（P＜0.05）。结论 小儿抗病毒颗粒具有明显的解热祛痰、镇咳平喘作用。     

仙鹤草乙醇提取物抗炎镇痛作用的实验研究

目的　观察仙鹤草乙醇提取物的抗炎镇痛作用。方法　采用二甲苯致炎法、酒石酸锑钾扭体法和热板法观察仙鹤草乙醇提取物连续给药 (相当于生药 8 0、16 0 g/kg ,ig ,qd× 5 )后对小鼠的抗炎镇痛作用。结果　仙鹤草乙醇提取物能显著抑制二甲苯引起的小鼠耳廓炎性肿胀 ,对热板所致的小鼠疼痛和酒石酸锑钾所致的小鼠扭体反应均有显著抑制作用。结论　仙鹤草乙醇提取物具有显著的抗炎镇痛作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.