上海正常人群谷胱甘肽S—转移酶T1纯合缺损

人类谷胱甘肽Ｓ－转移酶是一类重要的Ⅱ相反应代谢酶，它不仅与环境有害物质的减毒代谢有时也与活性代谢过程有关，胞浆谷胱甘肽Ｓ－转移酶ＧＳＴＴ１表现的遗传多态性主要是由相关编码基因的缺失造成的。本文通过由人β－珠蛋白基因片段作内标控制对人血有核细胞ＤＮＡ进行ＧＳＴＴ１等位基因专一（Ａｌｌｅｌｅ－ｓｐｅｃｉｆｉｃ）ＰＣＲ扩增的方法，对２２６名上海市健康常住居民进行ＧＳＴＴ１基因型检测。结果表明５０．３％（     

上海地区白血病谷胱甘肽S-转移酶T1和M1基因多态性分析

目的 研究谷胱甘肽S-转移酶(GSTs)T1、M1基因多态形态在上海地区白血病患者中的频率分布特征,探讨不同基因多态形态与白血病易感性之间的联系。方法 采用等位基因特异聚合酶链反应(AS-PCR)方法对上海地区61名白血病患者进行GSTF1、M1基因型分析,比较不同基因型在白血病组和同一地区正常人群组之间的分布频率差异,并就疾病类型、年龄和性别进行分层比较。结果 白血病组和正常对照组之间,GSTT10／0基因型以及GSTT10／0-GSTM10／0联合基因型的分布频率差异有显著性,缺损基因型在白血病组显著升高。进行疾病类型、性别以及年龄分层后,这种趋势在男性和低年龄(≤30岁)急性淋巴细胞白血病患者中依然存在。结论 GSTF10／0基因型和GSTT10／0-GSTM10／0联合基因型可能是罹患白血病的风险因子。     

微粒体谷胱甘肽S-转移酶1在恶性肿瘤中的研究进展

微粒体谷胱甘肽S-转移酶1(microsomal glutathione S-transferase 1,MGST1)是谷胱甘肽S-转移酶(glutathione S-transferase, GST)超家族和花生四烯酸与谷胱甘肽代谢中的膜相关蛋白(membrane-associated proteins in eicosanoid and glutathione metabolism, MAPEG)超家族的共同成员,它通过催化外源性物质的II相解毒过程,从而保护细胞膜免受氧化应激的损伤。众多研究发现MGST1与恶性肿瘤的发生发展密切相关,有望成为癌症治疗的新型分子靶点。本文就MGST1在恶性肿瘤中的研究进展予以综述。     

谷胱甘肽S-转移酶A1在肝癌中的异常表达

目的探讨GSTA1基因在肝癌形成中的作用。方法应用RT-PCR方法检测35例AFB1诱发的树鼩肝癌、癌旁和癌前组织,以及35例人肝癌和癌组织中的GSTA1mRNA表达情况;应用免疫组化方法检测以上组织的GSTA1蛋白表达情况。结果树鼩肝癌组织的GSTA1mRNA和蛋白表达水平均低于癌旁及癌前组织;人肝癌组织的GSTA1mRNA表达水平、蛋白阳性表达率和表达综合得分均显著低于癌旁组织(分别为P<0.001、P<0.05和P<0.001)。结论GSTA1可能是肝癌发生发展的重要相关基因之一;在mRNA水平和蛋白质水平动态观察相关基因在肝癌形成过程中的表达变化有助于阐释肝癌发生的分子机制。     

云南籍几个正常人群间GSTM1基因多态性的比较研究

目的: 针对云南曲靖地区宣威县、富源县及非曲靖地区3组正常人群进行GSTM1基因型多态性的比较研究.方法:采用聚合酶链反应(Polymerase chain reaction,PCR)技术对相关人群进行基因型鉴定.结果:GSTM1纯合缺失基因型(0/0)的频率在宣威人群为63.6%(n＝33),富源人群为64.5%(n＝31),非曲靖人群为68.5%(n＝35).结论:GSTM1基因型多态性在3个正常人群中的分布情况均一.该研究为了解特定地区肺癌与外源物代谢解毒基因之间的可能联系提供了一些基础数据.     

谷胱甘肽S-转移酶P1对小鼠肺癌细胞放射敏感性调控作用

目的:探讨谷胱甘肽S-转移酶P1(GSTP1)对小鼠路易斯肺癌细胞放射敏感性的调控作用。方法:使用shRNA干扰表达慢病毒和阴性对照病毒分别感染路易斯肺癌(LLC)细胞,并筛选出稳转株;应用RT-PCR和蛋白免疫印迹(WB)法检测LLC细胞GSTP1 mRNA和蛋白质表达水平,验证敲低效果;使用细胞增殖检测(CCK-8...     

谷胱甘肽硫转移酶M1与T1基因缺失增加鼻咽癌易感性(英文)

目的在中国西南的广西壮族自治区的鼻咽癌高发区内研究谷胱甘肽硫转移酶 M1与 T1遗传多态性与鼻咽癌易感的相关性。方法病例与对照研究这些酶的遗传多态性(GSTM1和 GSTT1零基因型),鼻咽癌总数为127例,对照207例。结果 GSTM1和 GSqT1零基因型的频数在 NPC 患者中较高,差异达统计学意义(P<0.001)。结论鼻咽癌是广西最常见癌症,GST 酶与多种环境致癌物的解毒相关,同合子缺失 GSTM1和 GSTT1与数种癌相关,生鼻咽癌危险性已知与环境因素如吸烟和 EB病毒感染相关联,我们的结果提示 GSTM1和 GSTF1缺失多态性与增加鼻咽癌易感性相关,若两种解毒酶基因同时缺失对鼻咽癌易感受性意义更重要。     

谷胱甘肽硫转移酶M1与Tl基因缺失增加鼻咽癌易感性

目的在中国西南的广西壮族自治区的鼻咽癌高发区内研究谷胱甘肽硫转移酶M1与T1遗传多态性与鼻咽癌易感的相关性。方法病例与对照研究这些酶的遗传多态性(GSTM1和GSTT1零基因型),鼻咽癌总数为127例,对照207例。结果GSTM1和GSTT1零基因型的频数在NPC患者中较高,差异达统计学意义(P<0.001)。结论鼻咽癌是广西最常见癌症,GST酶与多种环境致癌物的解毒相关,同合子缺失GSTM1和GSTT1与数种癌相关,生鼻咽癌危险性已知与环境因素如吸烟和EB病毒感染相关联,我们的结果提示GSTM1和GSTT1缺失多态性与增加鼻咽癌易感性相关,若两种解毒酶基因同时缺失对鼻咽癌易感受性意义更重要。     

肝细胞癌患者的谷胱甘肽转移酶M1基因型分析

目的 研究广西黄曲霉毒素（AFB1）高危区肝细胞癌（HCC）患者谷胱甘肽转移酶（GST）M1的基因型。方法 用GSTM1特异引物和PCR方法检测末稍血白细胞中的GSTM1基因,研究对象为：病理确诊为HCC患者162例（HCC组）、当地无癌成年人177例（对照I组）和HCC低发区北京、河北青年40例（对照Ⅱ组）。结果 GSTM1基因缺失（GSTM1－null)型在HCC组为102例,占63％;对照I组为92例,占52％;对照Ⅱ组为13例,占33％。各组相比,差异有显著性。结论 GSTM1为AFB1Ⅱ期代谢解毒酶,遗传性缺失这种酶的个体对HCC的遗传易感性增高。广西HCC高发的因素,既有遗传易感性,又有AFB1高污染的环境因素,还有致癌病毒HBV和HCV等的协同致癌作用。     

STUDY OF THE DELETION MUTATION OF GLUTATHIONE S TRANSFERASE M1 GENE AND ITS ROLE IN SUSCEPTIBILITY TO HEPATOCELLULAR CARCINOMA

The severe aflatoxin B1 contamination as the main cause of hepatocellular carcinoma in Guangxi had been proposed by many researches. The 8, 9-epoxide aflatoxin is the metabolized product of aflatoxin B1 in liver and can covalently bind to guanine forming AFB1-N7-Gua adduct which was believed to induce G-T transversion mutation in experimental animals, as well as to activate protooncogene ras and result in p53 gene aberration. In general thinking, the detoxification of carcinogens with epox…     

Glutathione S-Transferase T1 and M1 Polymorphisms and Risk of Uterine Cervical Lesions in Women from Central Serbia

The aim of this study was to investigate the frequencies of GSTT1 and GSTM1 deletion polymorphismsin newly-diagnosed patients with uterine cervical lesions from central Serbia. Polymorphisms of GST geneswere genotyped in 97 patients with cervical lesions and 50 healthy women using a multiplex polymerase chainreaction (PCR). The GSTM1 null genotype was significantly more prominent among the patients than in controls(74.2% vs 56.0%), the risk associated with lesions being almost 2.3-fold increased (OR=2.26, 95%CI=1.10-4.65,p=0.03) and 3.17-fold higher in patients above >45 years old (95%CI=1.02-9.79, p=0.04). The analysis of the twogenotypes demonstrated that GSTM1 null genotype significantly increased risk only for low grade squamousintraepithelial lesion-LSIL (OR=2.81, 95%CI=1.03-7.68, p=0.04). GSTT1 null genotype or different genotypecombinations were not found to be risk factors, irrespective to lesion stages, age or smoking. We found that therisk of cervical lesions might be significantly related to the GSTM1 null genotype, especially in women agedabove 45 years. Furthermore, the GSTM1 polymorphism might have greater role in development of early stagelesions.     

AMPLIFICATION OF THE hst-1 GENE IN HUMAN ESOPHAGEAL CARCINOMAS

The hst -1 gene, previously designated as the hst gene, and seven other oncogenes were examined for possible structural changes in esophageal, gastric and colorectal carcinomas by Southern blot hybridization. The hst -1 gene was amplified in eight (42.1%) of the nineteen esophageal squamous cell carcinomas and in all four metastatic tumors of lymph nodes. The degree of amplification ranged from two to eight times. Coamplification of the hst -1 and c- erbB -1 gene was found in one case of esophageal carcinoma. However, no amplification of the hst -1 gene was detected in gastric and colorectal carcinomas.     

LEVELS OF GLUTATHIONES TRANSFERASE π mRNA IN HUMAN LUNG CANCER CELL LINES CORRELATE WITH THE RESISTANCE TO CISPLATIN AND CARBOPLATIN

The amounts of mRNA for glutathione S transferase π (GST π) were significantly lower in 3 human small cell lung cancer (SCLC) cell lines than in 3 non small cell lung cancer (NSCLC) cell lines. The sensitivities of the 3 SCLC cell lines to cisplatin and carboplatin were much higher than those of the 3 NSCLC cell lines. These results indicate that low levels of GST π mRNA expression in SCLC cell lines inversely correlate to high sensitivity to cisplatin and carboplatin, and further suggest that GST π may play an important role in intracellular inactivation of these drugs.     

遗传性乳腺癌易感基因BRCA1在女性患者表达的研究

目的 探讨中国人群中乳腺癌与乳腺癌基因ＢＲＣＡ１的关系。方法 应用聚合酶链反应－单链构象多态性分析技术（ＰＣＲ－ＳＳＣＰ）和ＤＮＡ序列测定技术对上海地区５０例良笥乳腺肿瘤和２５例恶性乳腺肿瘤患者和香港地区１３０例乳腺癌患者进行了ＢＲＣＡ１基因表达的研究,并结合免疫组织化学分析其雌激素受体（ＥＲ）,孕激素受体（ＰＲ０水平。     

Colorectal cancer and genetic polymorphisms of CYP1A1, GSTM1 and GSTT1: a case-control study in the Grampian region of Scotland

Cytochrome P-450 CYP1A1 is involved in the metabolism of polycyclic aromatic hydrocarbons (PAHs) that are derived from meat intake and tobacco smoking. Expression of the CYP1A1 gene is induced by compounds present in cruciferous vegetables. The glutathione S-transferases play a central role in the detoxification of carcinogens, including PAHs. We investigated the association between colorectal cancer and three variants (CYP1A1*2A, CYP1A1*2C, CYP1A1*4) of the CYP1A1 gene, and homozygosity for the null deletion of the GSTM1 and GSTT1 genes, and the joint effects of these genotypes and smoking, meat intake and intake of green leafy vegetables in a population-based study of 264 cases and 408 controls in Northeast Scotland. There was an inverse association with the CYP1A1*4 (m4) variant (OR 0.3, 95% CI 0.13-0.70). The OR for the CYP1A1*2C (m2) variant was 1.3 (95% CI 0.59-2.91), which is similar to a combined estimate for previous studies (OR 1.2, 95% CI 0.95-1.41). We observed no association with the CYP1A1*2A (m1) variant, or the GSTM1 and GSTT1 polymorphisms. Significant interactions between all 3 CYP1A1 variants and meat intake, and between the m1 and m2 variants and intake of green leafy vegetables, were observed. There was no evidence of interaction between CYP1A1 and smoking, and no evidence of interaction between the GSTM1 or GSTT1 polymorphisms and smoking, meat intake, green leafy vegetable intake, CYP1A1 variants or each other.     

阿霉素诱导细胞凋亡时的基因调控

目的 :探讨阿霉素诱导细胞凋亡的分子机制。方法 :通过光镜和电镜观察阿霉素作用不同时间后的形态学改变 ,DNA琼脂糖凝胶电泳、流式细胞仪分析 DNA含量和细胞周期 ,并检测细胞 p5 3基因蛋白表达 ;免疫组织化学法检测 bcl- 2基因蛋白的表达。结果 :阿霉素作用于 MEC- 1细胞后出现了细胞凋亡的特征 ,p5 3蛋白表达的荧光指数 (FI)值分别为 0 .2 0± 0 .0 4、0 .42± 0 .11、1.6 7± 0 .17,bcl- 2基因蛋白表达逐步降低。结论 :p5 3基因和bcl- 2基因参与凋亡过程可能是阿霉素诱导 m Ec- 1细胞凋亡的主要机制     

THE ROLE OF VCAM-1/VLA-4 IN THE ACTIVATION OF ALLOGENIC T CELLS BY MURINE MACROPHAGES

ＴＨＥＲＯＬＥＯＦＶＣＡＭ１／ＶＬＡ４ＩＮＴＨＥＡＣＴＩＶＡＴＩＯＮＯＦＡＬＬＯＧＥＮＩＣＴＣＥＬＬＳＢＹＭＵＲＩＮＥＭＡＣＲＯＰＨＡＧＥＳＨｅＬｏｎｇ何龙ＣａｏＸｕｅｔａｏ曹雪涛ＺｈａｎｇＷｅｉｐｉｎｇ章卫平ＣｈｅｎＧｕｏｙｏｕ陈国友Ｚｈ...