口腔癌患者唾液中透明质酸含量的检测及意义

目的:检测口腔癌患者唾液中透明质酸(HA)的含量,探讨HA对口腔癌辅助诊断和治疗效果监测的价值。方法:选择住院治疗的口腔癌患者36例,健康人20例,采用酶联免疫吸附试验法(ELISA)测定所有标本唾液中的HA浓度。结果:治疗前口腔癌患者唾液中HA含量显著高于健康人(P<0.05),治疗后口腔癌患者唾液中HA含量低于治疗前,但差异不显著(P>0.05)。结论:唾液中HA含量测定对口腔癌可能有辅助诊断价值。唾液中HA的含量变化可作为判断肿瘤预后的参考,但不能用于治疗效果的监测。     

口腔鳞状细胞癌患者唾液中透明质酸酶的活性分析

目的 研究口腔鳞状细胞癌(OSCC)患者唾液中透明质酸酶(HAase)活性的变化.方法 采用类似酶联免疫吸附原理(ELISA-like)方法,研究HAase在正常人、OSCC患者、口腔良性肿瘤及瘤样病变患者唾液中的活性变化.结果 OSCC患者唾液中HAase活性为良性肿瘤患者和正常人唾液中HAase活性的2～3倍,而正常个体与良性肿瘤及瘤样病变患者唾液中HAase活性之间差异无统计学意义(P>0.05).以5.0 U/L为界值,根据HAase的活性检测OSCC的灵敏度为79.17%,特异度为65.00%;HAase活性与OSCC临床分期和颈淋巴转移正相关(P<0.01),而与病理分级无关(P>0.05).结论 HAase与OSCC的生长和侵袭密切相关,以唾液中HAase的活性为标记物检测口腔鳞状细胞癌,目前尚不能作为OSCC诊断的独立指标.     

正常人泪液与唾液成份的比较

目的：比较正常人泪液、颌下腺及腮腺分泌液各种成份之间的差异，探讨用自身唾液代替泪液的可行性、可能发生的问题及解决方法。方法：常规生化检查方法测定30例正常人颌下腺分泌液、腮腺分泌液及泪液成份并进行比较。结果：正常人唾液和泪液成份比较显示，泪液中各项成份，唾液中均可检出，虽然颌下腺分泌液、腮腺分泌液及泪液某些成份上存在差异。但除钠含量及渗透压的值差异较大外，其余各项值的均值偏离不大。正常人泪液中也含有很高浓度的淀粉酶。结论：可以用自身颌下腺分泌的唾液代替泪液。     

唾液中一氧化氮含量与牙周炎的相关性研究

目的:研究牙周炎患者唾液中一氧化氮(NO)含量与牙周炎各种临床指标的相关性,探讨一氧化氮在牙周炎发展过程中可能发挥的作用.方法:选择牙周健康者28人(对照组),慢性牙周炎32人(实验组),用Griess反应测定牙周基础治疗前后唾液中亚硝酸盐含量,间接反映NO水平,分析牙周临床指标与NO含量的关系.结果:实验组唾液中NO含量较对照组显著增加,牙周炎患者唾液NO含量与牙周探诊深度,附着水平之间有显著正相关.结论:NO的产生与牙周炎症过程有关.牙周基础治疗后,随着局部炎症逐渐减轻,唾液NO含量呈下降趋势.     

口腔颌面部恶性肿瘤患者血清中透明质酸含量的检测

目的　检测口腔颌面部恶性肿瘤患者血清中透明质酸(HA)的含量,探讨HA对口腔颌面部恶性肿瘤辅助诊断和治疗效果监测的价值。方法　选择住院治疗的口腔颌面部恶性肿瘤患者44例,健康人24例。肿瘤患者于手术前或化疗开始前采血,其中20例患者于手术后或化疗结束后再次采血;24例健康人于常规体检时采血。用放射免疫试验测定所有标本血清中的HA浓度。结果　治疗前口腔颌面部恶性肿瘤患者血清中HA含量显著高于健康人(P<0·05),口腔颌面部鳞癌患者血清中HA含量显著高于健康人(P<0·05),涎腺腺样囊性癌患者血清中HA 含量与健康人无显著差异(P>0·05)。口腔颌面部恶性肿瘤Ⅲ、Ⅳ期患者血清中HA含量显著高于Ⅰ、Ⅱ期患者和健康人(P<0·05)。口腔颌面部恶性肿瘤患者治疗后血清中HA含量低于治疗前,但差异不显著(P>0·05)。结论　HA含量测定对口腔颌面部恶性肿瘤可能有辅助诊断价值。血清中HA的含量变化可作为肿瘤分期和判断肿瘤预后的参考,但不能用于治疗效果的监测。     

透明质酸及透明质酸酶在肿瘤生长和转移中作用的研究进展

摘要透明质酸是细胞外基质中一种非硫酸化糖胺聚糖。研究发现,透明质酸及透明质酸酶的表达水平在许多肿瘤组织中显著升高,二者在肿瘤的发展和转移中起重要作用。本文对透明质酸及透明质酸酶对肿瘤的作用及其在头颈肿瘤和其他肿瘤中的研究现状作一综述。     

感染齿龈内阿米巴的牙周炎患者唾液中酶和MDA的含量与作用

目的 探讨酶类和氧自由基在齿龈内阿米巴（Ｅｎｔａｍｏｅｂａ ｇｉｎｇｉｖａｌｉｓ,Ｅｇ）致牙周炎中的作用。方法 以自然流滴法分别收集感染Ｅｇ的牙周炎患者,无感染Ｅｇ的牙周炎患者及健康对照者三组的唾液,用生化方法检测唾液中的超氧化物歧化酶（ＳＯＤ）,酸性磷酸酶（ＡＣＰ０,过氧化氢酶（ＣＡＴ）活性和过氧化脂质产物丙二醛（ＭＤＡ）的含量。结果 有,无感染Ｅｇ的牙周炎两组的ＳＯＤ活性均低于正常组;感染Ｅｇ     

唾液肌酐和尿酸的酶法测定及其应用

目的　探讨唾液肌酐和尿酸的酶法测定及其与血清肌酐和尿酸的关系。方法　用肌氨酸氧化酶测定唾液肌酐 ,尿酸酶 -过氧化物酶法测尿酸 ,与血清肌酐和尿酸作对照分析。结果　健康人唾液肌酐和尿酸分别为 13.2 7± 5 .15 μmol/L和 10 8.2± 2 5 .4 μmol/L;唾液肌酐与血清肌酐浓度显著相关 (r =0 .88,P <0 .0 1) ,唾液尿酸与血清尿酸亦显著相关 (r=0 .5 3,P <0 .0 1)。结论　唾液肌酐浓度测定可用于慢性肾脏疾病经常性监测手段。     

Antioxidant capacity of human saliva and periodontal screening assessment in healthy adults

ObjectiveSaliva plays a pivotal role as an antioxidant system, and saliva antioxidant levels are reduced in patients with periodontal disease. Recently, a biochemical test able to determine saliva antioxidant levels was proposed as predictive for oral cavity diseases, but it was not clinically tested. In this preliminary study, we evaluated the relationships between Periodontal Screening and Recordings characteristics of patients and saliva antioxidant levels measures.DesignThirty-nine patients (12 men, 27 women; mean age, 46 years, SD 17) attending the dental hygiene unit of a Private Clinic underwent a Periodontal Screening and Recordings examination and a saliva antioxidant levels measurement using a biochemical commercial test. The results of the clinical periodontal examination were compared to those obtained by the saliva test.ResultsApproximately 70% of patients showed a low saliva antioxidant levels value, while the other patients had Optimal/Normal values. Thirteen patients (33%) resulted positive to Periodontal Screening and Recordings test. Using Periodontal Screening and Recordings values as gold standard, the saliva antioxidant levels test correctly classified 52.6% of patients; sensitivity was 84.6%, specificity was 36%.ConclusionsThe saliva antioxidant levels test had a good sensitivity when compared to the gold standard; this finding corroborates the hypothesis that alterations of the oral antioxidant levels are related to periodontal disease. The reduced specificity shows that saliva antioxidant levels test could detect alterations predisposing to periodontal disease before clinically evident aspects.     

透明质酸改性聚乳酸支架组织工程软骨的构建

目的 利用透明质酸( hyaluronic acid,HA)改性聚乳酸(polylactic acid,PLA)支架来构建同种异体组织工程软骨,分析探讨HA改性PLA支架构建组织工程软骨的可行性.方法 采用盐析法制备出高孔隙率的PLA支架,采用低浓度NaOH、碳化二亚胺和HA进行支架改性.支架预湿后接种第3代兔髁突软骨细胞悬液,体外培养1周后用于动物实验.软骨细胞-HA改性PLA支架复合体为实验组,软骨细胞-PLA支架复合体为对照组.分别于4、6、8周按照标记取出植入物,进行组织学检测.利用ImageJ软件进行免疫组化染色阳性强度的分析.结果 细胞-支架复合体植入后,未产生排斥反应.HE染色结果示软骨样组织形成.Masson染色及甲苯胺蓝染色结果显示染色逐渐加深.Ⅱ型胶原免疫组化染色随时间增加阳性强度增加,实验组4、6、8周时的免疫组化染色强度差异有统计学意义(F=26.68,P=0.000),对照组4、6、8周时的免疫组化染色强度差异有统计学意义(F=20.59,P=0.000).相同时间点实验组Ⅱ型胶原分泌强度高于对照组.结论 利用HA改性PLA支架可以构建同种异体软骨,且其质量高于未改性PLA.     

Rhinofilling with hyaluronic acid thought as a cartilage graft

IntroductionThere is great demand for nonsurgical aesthetic procedures. In this case series, the authors describe their stepwise technique of injecting hyaluronic acid (HA) into the nose to yield pleasing, stable results.Case seriesA total of 148 patients underwent minimally invasive rhinoplasty by injection of HA filler. Anatomic contraindications for the procedure were a large dorsal hump, extreme tip projection, or excessive nasal deviation. HA injections were made in a specific order to reshape and stabilize the nose; the surgical plan was conceptually similar to placement of cartilage grafts. Patients indicated their satisfaction with the surgical results on a visual analog scale. Immediately after the procedure, all patients had transient redness and slight swelling at the injection site; this resolved spontaneously by 24 h posttreatment. Vascular impairment developed in 1 patient and was managed with hyaluronidase. In general, patients expressed a high level of satisfaction. Thirty-two patients returned for a “touch-up” filler injection 1 year posttreatment.DiscussionNasal reshaping by HA injection can be carried out quickly, safely, and effectively with minimal patient discomfort or downtime. Care must be given to select patients who are indicated for this procedure, and meticulous pretreatment planning is necessary.     

透明质酸补充疗法对兔颅下颌关节盘前移位的作用

目的 了解透明质酸（HA）补充疗法对兔颅下颌关节盘前4移位不同时期的作用。方法 家兔18只,右侧颅下颌关节盘前移位动物模后,分为HA组（12只）和对照组（6只）。HA组平分为A、B、C三组,分别在术后0周、2周、7周作双侧关节腔内HA注射,再次注射与首次间隔1周。此后每组在离首次HA注射后2周和4周各处死2只。对照组相应按期处死。取标本作切片,行HE染色。结果 对照组出现了典型的关节内紊乱症状,晚期表现为骨关节病。HA组中,A组表现与正常关节组相似。B组髁状突全层增碍,进入适应性改建阶段,但未出现典型的结构破坏。C组经过修复阶段后,仍出现了与对照组相似的骨关节病病理表现。结论 HA粘弹补充疗法对早期的颅下凳关节紊乱病有明显的疗效,对刚开始发生退行性改变的关节软骨在一定程度上具有促进其修复作用。而已有严重病变尤其进入骨关节病阶段,HA仅能暂时缓解疾病的病理进程。     

Diclofenac in hyaluronic acid gel: an alternative treatment for actinic cheilitis

Objective

Actinic cheilitis (AC) is a precancerous lesion of the lip vermillion caused by prolonged exposure to ultraviolet light. The aim of this study was to evaluate the effect of 3% diclofenac in 2.5% hyaluronic acid gel in the treatment of AC.

Methods

Thirty-four patients with chronic AC were treated twice a day with topical diclofenac during a period of 30 to 180 days. The individuals were followed up every 15 days by means of clinical examination and digital photographic documentation.

Results

Of the 27 patients that completed the study, 12 (44%) showed complete remission of the whitish plaques and exfoliative areas, and 15 (56%) had partial remission of the clinical picture of cheilitis. The latter group was submitted to excision of the leukoplakic areas which diagnosis varied from mild to moderate epithelial dysplasia.

Conclusion

The results suggest a promising role for diclofenac in hyaluronic acid gel in the treatment of AC. This treatment has the advantages of not being invasive and showing few side effects.     

Efficacy of viscosupplementation with hyaluronic acid in temporomandibular disorders: A systematic review

Objective

To perform a systematic review of the viscosupplementation effectiveness with hyaluronic acid (HA) in the articular Temporomandibular Dysfunctions (TMDs) clinical management.

Effect of root conditioning agents hyaluronic acid,EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

BackgroundThe gold standard treatment of periodontal diseases is scaling and root planing (SRP). Different adjunctive root conditioning agents such as hyaluronic acid (HA), ethylenediaminetetraacetic acid (EDTA), and chlorhexidine (CHX) have been used with SRP to improve the smear layer removal and the healing of periodontal tissues. The aim of this study was to compare the effect of manual scaling with or without HA, EDTA, or CHX root conditioning on the attachment and viability of human gingival fibroblasts (GF).MethodsFifteen healthy single rooted teeth were co llected and divided randomly into a scaled (n = 12) and non-scaled control group (n = 3). The scaled roots were subdivided equally into four groups; the first group did not receive any chemical treatment, while the remaining groups were treated with the conditioning agents HA or 17% EDTA or 0.2% CHX gel. Gingival fibroblasts were seeded on the top of each root and incubated for 48 h to allow attachment to the roots. The viability of fibroblasts attached to the root surface was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay.ResultsThe cell viability was the highest in the scaled only group (p = 0.0001) while the lowest was in the scaled with EDTA group (p > 0.05). The scaled group was the highest followed by the HA and CHX groups, while the EDTA group showed the lowest mean value.ConclusionSRP remains the superior method for regaining cell attachment to the root surface, leading to better periodontal health, and adjunctive therapies did not enhance the GF attachment to the root surface beyond the effect of SRP. Further studies are needed to investigate the effect of root conditioning agents on periodontally diseased teeth in vitro and compare them in vivo.     

Effect of hyaluronic acid on the osseointegration of dental implants

The mechanism of osseointegration is related to many factors, including the quality of the bone, the biocompatibility and surface characteristics of the implant material, the surgical technique, and functional loading. The purpose of this study was to investigate the effects of hyaluronic acid gel on the osseointegration of implants placed in defined areas of the mandible in rabbits. Hyaluronic acid is known to have an osteoinductive effect during regeneration of bony defects, and we thought that it might also have a favourable effect on osseointegration, a specialised mechanism to heal bone. Ten New Zealand rabbits aged 10 weeks and weighing 2.5–3.0 kg were used, and sites for implants that were far enough from the apices of the teeth in the mandibular molar area were chosen. Two cavities were prepared in each rabbit, one (anterior) for the control implant, and one (posterior) for the implant with hyaluronic acid gel (Medical Instinct GmbH, Bovenden). New bone and the osteoid matrix content around the dental implants were evaluated histologically and histomorphometrically two months after the operation, and no significant difference was found between the two groups.