吸烟对高原成年男性红细胞免疫功能的影响

本文观察了194人吸烟者和66人不吸烟者的红细胞C_3b受体花环,红细胞免疫复合物花环及协同肿瘤红细胞花环。结果显示,吸烟者红细胞C_3b受体花环及协同肿瘤红细胞花环低于不吸烟者(P<0.01和P>0.05),红细胞免疫复合物花环则高于不吸烟者(P<0.01)。随着吸烟量的增加和吸烟时间的增长,红细胞C_3b受体花环和协同肿瘤红细胞花环呈降低趋势,而红细胞免疫复合物花环呈升高趋势。提示长时间过量吸烟将影响红细胞清除免疫复合物,影响红细胞粘附围攻肿瘤细胞的能力。     

西宁地区恶性肿瘤患者的红细胞免疫功能研究

目的：研究西宁地区（海拔2260m）恶性肿瘤的红细胞免疫功能的相关内容。方法：按文献^[2]介绍采用郭峰建立的最常用的三种检测指标和方法。结果：肿瘤病人红细胞C3b受体花环R-C3bR、红细胞免疫粘附肿瘤细胞花环R-TRR低下，与健康人之间有显著性差别，红细胞免疫复合物IC花环R-ICR无统计学差别，肿瘤患者的R-TRR男女之间有显著性差别。各种肿瘤之间三种花环率无统计学差别。化疗前后三种花环率无统计学差别。肿瘤无转移与有转移三种花环率无统计学差别。手术前后三种花环率无统计学差别。结论：西宁地区恶性肿瘤病人的红细胞免疫功能R-C3bR、R-TRR低下，分别为13．4762±3．597，12．0543±5．6095，R-ICR为4.6131±3．4010，与健康人无统计学差别。     

高压氧对鼠血细胞免疫功能的影响（论著）

目的：了解高压氧对免疫功能的影响。方法：高压氧作用后，摘眼球取血，对正常和荷瘤Ｂａｌｂ／Ｃ小鼠的ＲＢＣ－Ｃ３ｂ受体花环率，ＲＢＣ免疫复合物花环率及肿瘤细胞。淋巴细胞花环率进行了观察。结果：（１）高压氧处理组的ＲＢＣ－Ｃ３ｂ受体花环率明显高于对照组（Ｐ＝０．００７）；（２）肿瘤高压氧处理组的ＲＢＣ－Ｃ３６受体花环率明显高于单纯肿瘤接种组（Ｐ＝０．０２１）。（３）肿瘤高压氧处理组的肿瘤细胞－淋巴细胞花环率明显高于对照组（Ｐ＝０．００５）；（４）肿瘤高压氧处理组的肿瘤细胞：淋巴细胞花环率明显高于单纯高压氧处理组（Ｐ＝０．０３６）。（５）各组的ＲＢＣ－ＩＣ花环率无明显变化。结论：（１）压力为０．２ＭＰａ（２ＡＴＡ），氧浓度为８７％的高压氧不仅可以提高正常小鼠的免疫功能，而且可以提高荷瘤小鼠的免疫功能；（２）该条件的高压氧可使移植瘤引起的免疫功能低下程度得到减轻。     

114例消化系统恶性肿瘤患者红细胞免疫功能测定的探讨

本文对114例各类消化系统恶性肿瘤及48例健康成人红细胞免疫功能调节因子进行了观察比较,经统计学处理与正常人相比,消化系统各肿瘤红细胞C_(3b)受体花环促进率(RFER)降低,而红细胞C_(3b)受体花环抑制率(RFIR)明显升高(P<0.01)。这不仅与肿瘤细胞产生的某些抑制因子有关,而且与机体红细胞免疫功能调节紊乱和红细胞粘附IC增多及SOD活性下降有关。     

高原地区肝功能异常者红细胞免疫功能的观察

目的：观察则察地区２７例和甘德地区３０例肝功能异常的患者红细胞免疫功能测定；方法：采用红细胞Ｃ３ｂ受体花环率和复合花环经试验；结果：两个地区肝功能异常患者的红细胞Ｃ３ｂ受体花环率均低于正常人对照组，而红细胞免疫复合物花环经高于正常人对照组；结论：在高原地区肝功能异常患者在治疗的同时，应纠正和改善红细胞免疫调节功能。     

高原地区进展期胃癌患者红细胞免疫功能和T淋巴细胞亚群的研究

目的：研究高原地区进展期胃癌（AGC）患者的红细胞免疫功能和淋巴细胞免疫功能;方法：对我科住院手术治疗的32例进展期胃癌患者作了红细胞C3b受体花环（RBC－C3bRR)、红细胞免疫复合物花环（RBC－ICR）及淋巴细胞亚群CD3、CD4、CD8、CD4／CD8的检测,并与20例正常人结果进行比较;结果：高原地区进展期胃癌患者RBC－C3bRR,CD3,CD4,CD8、CD4／CD8比值均低于正常人（P＜0．01）,RBCICR高于正常人（P＜0．01）;结论：高原地区进展期胃癌患者红细胞免疫系统与淋巴细胞免疫系统均处于抑制状态,2个免疫系统的改变有着密切的关系,对高原地区进展期癌患者应给予免疫支持。     

高原地区胃癌患者红细胞免疫功能变化的临床研究

目的：探讨高原地区胃癌患者红细胞免疫功能情况;方法：分别测定胃癌病人在手术前后红细胞Ｃ３ｂ花环形成试验受体（Ｃ３ｂ ＲＲＴ）以及红细胞免疫复合物花环试验（ＩＣＲＴ）,其结果与正常人做对比;结果：胃癌病人红细胞Ｃ３ｂ花环促进率术前下降,明显低于正常对照组（Ｐ〈０．０１）,术后回升。红细胞免疫复合物花环率在手术前高于正常对照组,术后明显下降,与术前相比有明显差异（Ｐ〈０．０１）;结论：高原地区居民患胃     

高原肺水肿患者红细胞免疫粘附功能变化及其意义

本文观察了29例肺水肿患者的红细胞免疫粘附功能及调节因子水平，与34例初入高原健康人及25例急性高原反应患者进行了比较，他们分别于治疗前、恢复期、痊愈期抽血通过红细胞C3b受体花环率试验（RCR）、红细胞免疫复合物花环率试验（RICR）、红细胞C3b受体花环促进率试验（RFER）、红细胞C3b受体花环抑制率试验（RFIR）和PEG沉淀法检测，发现高原肺水肿患者RCR、RICR及RFER均降低、RFIR升高，血清中CIC则升高，急性高原反应患者RCR和血清中CIC接近对照组.RICR明显升高，RFER和RFIR均与正常对照组接近，表明高原肺水…     

IL-2对辐射损伤小鼠红细胞免疫粘附功能的影响

机体的免疫系统对辐射很敏感,经照射后淋巴细胞的多种功能受到损伤,对红细胞免疫粘附功能也有明显的抑制作用。ＩＬ-2可使免疫缺陷患者免疫功能重建,能使某些放疗、化疗造成的免疫功能降低得到逆转。红细胞Ｃ3ｂ受体花环与红细胞免疫复合物花环是反映红细胞免疫功能的重要指标。作者将小鼠一次性60Ｃｏγ射线照射后,给予ＩＬ-2治疗,观察其对红细胞Ｃ3ｂ受体花环及免疫复合物花环的逆转作用。一、材料和方法1白细胞介素-2(ＩＬ-2):105Ｕ/ｍｌ,军事医学科学院出品。2动物:昆明种雄性小鼠,体重20～22ｇ,60只,山东鲁抗医药股份有限公司实验动物…     

中度高原地区体育锻炼对老年人红细胞免疫和CIC的影响

观察中度高原（２２６０ｍ）地区，体育锻炼对老年人红细胞克疫粘附（ＲＣＩＡ）功能和循环免疫复合物（ＣＩＣ）水平的影响，方法：ＲＣＩＡ功能，采用红细胞Ｃ２ｂ受体花环率和复合物花环率试验，ＣＩＣ采用ＰＥＧ半定量法。结果：运动组老年人红细胞Ｃ３ｂ受体花环率明显升高（Ｐ＜０．０１），而且ＣＩＣ明显下降（Ｐ＜０．０２）．结论：中度高原地区老年人体育锻炼可增强红细胞免疫粘附功能，使红细胞Ｃ３ｂ全体花环率升高，减少由ＣＩＣ所致的疾病，有利于抗衰老。     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.     

Prevalence and severity of hip and groin pain in sub‐elite male football: a cross‐sectional cohort study of 695 players

The purpose of this study was twofold: (a) to investigate the prevalence of hip and groin pain in sub‐elite male adult football in Denmark and (b) to explore the association between prevalence and duration of hip and groin pain in the previous season with the Copenhagen Hip and Groin Outcome Score (HAGOS) in the beginning of the new season. In total 695 respondents from 40 teams (Division 1–4) were included. Players completed in the beginning of the new season (July–Sept 2011) a self‐reported paper questionnaire on hip and/or groin pain during the previous season and HAGOS. In total 49% (95% CI: 45–52%) reported hip and/or groin pain during the previous season. Of these, 31% (95% CI: 26–36%) reported pain for >6 weeks. Players with the longest duration of pain during the previous season had the lowest HAGOS scores, when assessed at the beginning of the new season, P < 0.001. This study documents that half of sub‐elite male adult football players report pain in the hip and/or groin during a football season. The football players with the longest duration of pain in previous season displayed the lowest HAGOS scores in the beginning of the new season.     

Bronchial stump fistula :treatment with covered retrievable hinged metallic stents-preliminary clinical experience

Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula （BSF）. Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types （A and B） of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7 （87.5%） of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple, safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. （J Intervent Radiol, 2007, 16： 253-257）