舌大小与开He相关性的研究

目的 研究舌大小与开He之间的关系。方法 采用B型超声成像方法分别测量正常He与开He患者的舌大小，通过牙He与正常He舌大小的比较，研究舌大小与开He的相关性。结果 开He患者的舌大小测量结果均值大于正常He者，而且这种差别有显著的统计学意义。结论 与正常He者比较，开He患者存在舌过大的特性。     

舌侧二维矫治器的临床应用初步研究

目的 总结9例用舌侧二维矫治器（德国非凡公司）完成正畸治疗的不拔牙病例,为正畸医生应用这一技术提供治疗参考.方法 9名不拔牙矫治患者,采用舌侧二维托槽进行矫治,分析临床使用情况,总结操作流程及应用体会,并对治疗前后的头颅侧位片进行回顾性分析,评价其对牙（牙合）和面型的影响.结果 9例完成病例在矫治期间获得最大美观的同时,在3～4个月时间内实现排齐整平,下颌牙列排齐时间及效果优于上颌,托槽脱落率15.6%,下前牙转矩增加2.1±0.8°.结论 舌侧二维矫治器可以满足临床美观矫治的需要,应用时上颌需要弯制较多补偿曲,下切牙有唇倾倾向.     

舌侧二维矫治器的临床应用初步研究

Objective To summarize the application of lingual 2-D brackets in 9 nonextraction patients (Forestadent, German), and provide some helpful information for the development of a new technique. Methods The clinical application of lingual 2-D brackets was analyzed, and operational processes and experience were summarized. The patients' cephalograms were collected to evaluate the dental and profile changes resulting from the treatment. Results 9 finished cases achieved dental alignment and leveling in 3～4 months, with high esthetic acceptance. Additionally, lower arch was more easily controlled than upper arch. The failure rate of 2-D brackets were 15.6%. The torque of lower anterior teeth had increased 2.1±0.8°. Conclusions The use of lingual 2-D brackets can meet orthodontic clinical needs. Compensation bends were necessary in upper arch and the lower anterior teeth presented with proclined tendency.     

舌侧二维矫治器的临床应用初步研究

Objective To summarize the application of lingual 2-D brackets in 9 nonextraction patients (Forestadent, German), and provide some helpful information for the development of a new technique. Methods The clinical application of lingual 2-D brackets was analyzed, and operational processes and experience were summarized. The patients' cephalograms were collected to evaluate the dental and profile changes resulting from the treatment. Results 9 finished cases achieved dental alignment and leveling in 3～4 months, with high esthetic acceptance. Additionally, lower arch was more easily controlled than upper arch. The failure rate of 2-D brackets were 15.6%. The torque of lower anterior teeth had increased 2.1±0.8°. Conclusions The use of lingual 2-D brackets can meet orthodontic clinical needs. Compensation bends were necessary in upper arch and the lower anterior teeth presented with proclined tendency.     

牙(牙合)模型视频立体测量系统的建立与应用

目的 建立计算机辅助的牙颌模型立体分析测量系统并应用于临床。方法 将视频测量技术应用于口腔医学领域，系统由坐标控制场，两台CCD彩色摄像机，真彩色图象卡，高清晰度彩色监视器及计算机等设备组成，通过特定的测量软件完成模型测量分析。结果 系统经检测精度合乎临床要求，并应用本系统分别对正常He，安氏Ⅱ类1分类错He及安氏Ⅲ类错He的模型进行了研究。结论 模型视频立体测量系统是一套具有临床实用价值及推广意义的计算机诊断系统。     

正常和异常舌组织MR弥散加权成像的评价

目的:评价正常和异常舌组织的磁共振-弥散加权成像(MR-DWI)表现,比较正常和病变舌在不同b值条件下的表观弥散系数(ADC值)差异.方法:分别对15例正常(志愿者)舌组织和79例经手术病理证实的舌部病变(良性19例,恶性60例)行术前MR-DWI检查,在b值分别取500s/mm<'2>和1000s/mm<'2>的情况下计算各自的ADC均值.回顾性比较正常舌组织与舌部病变之间在不同b值下的ADC均值异同.采用SPSS10.0软件包对实验数据进行t检验.结果:①正常舌组织与舌病变组织的ADC均值之间存在显著差异(P<0.01);②舌良性病变ADC均值高于舌恶性病变(P<0.05);③舌良性病变ADC均值高于正常舌及病变周围舌组织(P<0.05);④舌恶性病变ADC均值高于正常舌(P<0.05),但与病变周围舌组织之间无显著性差异(P>0.05).结论:MR-DWI能显示正常和异常舌组织之间的差异,具有一定的临床价值.     

骨性Ⅱ,Ⅲ类错舌大小位置及姿势位的比较研究

目的：比较骨性Ⅱ类与Ⅲ类错（牙合）畸形舌的大小及姿势位置,探讨舌与矢状向骨性错（牙合）畸形的关系.方法：选用Ⅱ类错（牙合）畸形病例20例,其中男为9例,女为11例,平均年龄在13岁.Ⅲ类错（牙合）畸形病例20例,其中男为11例,女为9例,平均年龄在15岁.头影测量进行舌总面积、舌体面积、舌根面积、（牙合）平面以上舌面积、舌体的位置（S0、UO）以及舌体高度及长度及舌体的姿势位（ET、HET、Ltg1-7）的比较分析.结果：1）舌体的前后位置在两种畸形之间未见显著差异.2）舌体的上部形态（Ltg1-7）及（牙合）平面以上的面积二者之间没有显著差异.3）Ⅲ类错（牙合）畸形病例的舌总面积及舌体面积大于Ⅱ类错（牙合）畸形病例,舌根面积两类错（牙合）畸形之间无显著不同.4）下颌骨长度（Go-Gn）与舌总面积、舌体面积、舌根面积及舌体长度具有显著相关性.结论：Ⅲ类错（牙合）畸形与Ⅱ类错（牙合）畸形之间舌体矢状向大小不同,其与下颌骨长度存在密切相关性.     

骨性Ⅱ,Ⅲ类错舌大小位置及姿势位的比较研究

目的:比较骨性Ⅱ类与Ⅲ类错牙合畸形舌的大小及姿势位置,探讨舌与矢状向骨性错牙合畸形的关系。方法:选用Ⅱ类错牙合畸形病例20例,其中男为9例,女为11例,平均年龄在13岁。Ⅲ类错牙合畸形病例20例,其中男为11例,女为9例,平均年龄在15岁。头影测量进行舌总面积、舌体面积、舌根面积、牙合平面以上舌面积、舌体的位置(SO、UO)以及舌体高度及长度及舌体的姿势位(ET、HET、Ltg1-7)的比较分析。结果:1)舌体的前后位置在两种畸形之间未见显著差异。2)舌体的上部形态(Ltg1-7)及牙合平面以上的面积二者之间没有显著差异。3)Ⅲ类错牙合畸形病例的舌总面积及舌体面积大于Ⅱ类错牙合畸形病例,舌根面积两类错牙合畸形之间无显著不同。4)下颌骨长度(Go-Gn)与舌总面积、舌体面积、舌根面积及舌体长度具有显著相关性。结论:Ⅲ类错牙合畸形与Ⅱ类错牙合畸形之间舌体矢状向大小不同,其与下颌骨长度存在密切相关性。     

安氏Ⅲ类错He三维x线头影测量颅面结构的结构—微机辅助

本研究利用现有正，侧位ｘ线片和电子计算机辅助的ｘ线头影测量技术重构颅颌骨三维形态，并对４２例１１－１６岁前牙反Ｈｅ的患者进行研究，用ｔ检验，对正常ｈｅ组与反Ｈｅ组进行比较，分析此类错Ｈｅ长，宽、高方向上的异常。再用直线相关分析，对紫类畸形三维方向之间的相关性进行比较研究。找出它们之间的相互联系，探讨此类畸形成机制。     

女性前牙反He颅颌面软组织侧貌生长发育的二维有限元法研究—— …

目的：用限元法分析女性前牙反Ｈｅ患者从替牙期到恒牙期软组织侧貌生长发育特点。方法：用专门建立的有限元分析软件,由作者一人完成节点的输入。结果,大多数单元面积均有不同程度的增加,形态变化大于大小变化,鼻体部继续生长,以鼻尖部尤为明显,上唇唇珠变小,下唇基部和颏上部软组织长度和厚度增加,可能会进一步促使颏上部变长,颏唇沟点上移,反Ｈｅ面形加重。结论：用有限元法进行软组织侧貌分析是可行的,获得了女性前牙     

Usefulness of fat-suppressed Gd-enhanced MR imaging of tongue cancer

Purpose To evaluate the usefulness of the fat suppression technique for magnetic resonance imaging of oral tongue cancer. Methods One hundred and fourteen patients underwent both magnetic resonance imaging (MRI) and computed tomography (CT). All patients were clinically diagnosed as having oral tongue cancer shown to be squamous cell carcinoma histopathologically. We used two types of CT and six types of MRI scanning: plain CT, contrast enhanced CT, conventional T1w, conventional PDw, conventional T2w, fat-suppressed (FATS) T1w, Gd-enhanced conventional T1w, and Gd-enhanced FATS T1w images. The focus of our study was Gd-enhanced FATS T1w imaging. Results Tumor detection rates were as follows: Gd-enhanced FATS T1w MRI, 86.8%; conventional T2w MRI, 71.9%; conventional PDw MRI, 65.8%; Gd-enhanced conventional T1w MRI, 47.4%; contrast enhanced CT, 36.8%; T1w MRI, 20.2%; CT, 10.5%. There were 59 cases in which tumors were detected by Gd-enhanced FATS T1w MRI but not detected by contrast enhanced CT. Conclusions Gd-enhanced FATS T1w MRI was the best for the tumor detection and Gd-enhanced conventional T1w MRI was not useful in the diagnosis of the tongue cancer. CT imaging must not be the first choice for tumor detection in tongue cancer patients.     

两种粒径量子点在人舌鳞癌细胞中双色荧光成像的应用研究

目的 研究粒径655 nm和525nm的两种量子点(quantum dots,QDs)对人舌癌Tca8113细胞内2种热休克蛋白( heat shock protein,HSP)进行特异性荧光标记的成像效果和稳定性,为今后的连续动态观察提供实验依据.方法利用QD655nm和QD525nm,对人舌癌Tca8113细胞内HSP90蛋白和HSP70蛋白进行特异性双重荧光标记,激光连续照射2420391 ms,在共聚焦显微镜下同时观测人舌癌Tca8113细胞内的HSP90蛋白和HSP70蛋白表达及分布,用软件Leica Confocal Software测量量子点QD655nm和QD525nm的荧光信号强度变化.结果 激光共聚焦显微镜下可见人舌癌Tca8113细胞内HSP90蛋白和HSP70蛋白均有明显表达,分别表现为红色和绿色荧光,两种蛋白重叠处呈黄色荧光,在激光连续照射中,两种荧光有所衰减,其中QD655nm的荧光强度值下降相对较快、幅度相对较大.结论 量子点荧光标记技术能同时对人舌鳞癌细胞中HSP90蛋白和HSP70蛋白进行双重标记,而且QD655nm与QD525nm都具有较强的光稳定性,均可用于蛋白的长时间动态监测,其中QD525nm的稳定性更好.     

tankyrase 1反义逆转录病毒抑制人舌癌Tcca-8113细胞增殖的实验

目的研究反义tankyrase1逆转录病毒对舌癌细胞系TCCA-8113的端粒酶活性调节及对细胞生长的抑制作用,探讨以tankyrase1为靶点的舌癌基因治疗的可能性。方法构建含反义tankyrase1的逆转录病毒并转染包装细胞PT67后获得反义重组病毒,感染舌癌TCCA-8113细胞。采用RT-PCR检测tankyrase1表达,端粒酶重复扩增法(telomerase repeat amplification protocol,TRAP)检测端粒酶活性,绘制生长曲线了解细胞生长状态,倒置显微镜观察和流式细胞仪检测细胞凋亡。结果反义tankyrase1逆转录病毒作用后的舌癌细胞tankyrase1表达下降,端粒酶活性及细胞生长受到明显抑制,细胞出现凋亡。结论反义tankyrase1对舌癌TCCA-8113细胞的生长和端粒酶活性具有明显的抑制作用,有可能以tankyrase1为靶点对舌癌进行基因治疗。     

舌体组织瓣后置修复舌根癌切除后缺损

目的：探讨舌体组织瓣后置修复舌根癌切除后缺损的临床应用价值。方法：对6例舌根癌患者在原发灶切除后利用舌体组织瓣后置修复舌根缺损，术后随访评价功能恢复情况，结果：后置舌体组织瓣无明显萎缩，舌体运动基本正常，患者的语言及吞咽功能恢复比较满意，结论：舌体组织瓣后置修复舌根癌切除后缺损手术设计符合舌的解剖生理特点，手术相对简单，安全，术后功能恢复较好。     

全反式维甲酸对舌癌细胞株抗增殖作用的研究

目的：研究全反式维甲酸（ＡＴＲＡ）对舌癌细胞株的体外抗增殖作用。方法：经不同浓度全反式维甲酸（１０＾－７￣１０＾－５ｍｏｌ／Ｌ）处理舌癌细胞株后,应用流式细胞仪检测舌癌细胞细胞周期变化。结果：舌癌细胞株经１０＾－７￣１０＾－５ｍｏｌ／Ｌ全反式维甲酸处理１￣６ｄ后,Ｇ１／Ｇ０期比率呈上升趋势,Ｓ期和Ｇ２＋Ｍ期比率呈下降趋势,与对照组相比,经全反式到处理３ｄ和６ｄ的细胞,其Ｇ１／Ｇ０期比率明显上升（Ｐ     

Preoperative MRI for oral tongue squamous cell carcinoma: timing and correlation to histopathology

Magnetic resonance imaging (MRI) is an integral part of the evaluation of local and regional disease in tongue squamous cell carcinoma prior to surgery. The aim of this study was to evaluate the accuracy of MRI in assessing tumour dimensions, as well as the impact of the time-lag from diagnostic biopsy on the accuracy of MRI. The medical records of 64 patients with tongue carcinoma were reviewed retrospectively. Tumour maximum diameter and tumour depth of invasion were compared between pathology and MRI (T1- and T2-weighted). MRI-derived maximum tumour diameter and depth of invasion correlated strongly with histopathology: T1-weighted (r = 0.700 and r = 0.813, respectively) and T2-weighted (r = 0.734 and r = 0.834, respectively). A significant correlation was found between measurements on T1 and T2 MRI for both parameters (P = 0.955 and P = 0.984, respectively). The accuracy rate of MRI for T-staging of early tumours was low: 10% for T1 tumours; 39.3% for T2 tumours. A time-lag of less than 2 weeks between the diagnostic biopsy and MRI adversely affected the correlation of tumour dimensions. MRI is a reliable tool for evaluating tongue carcinoma; however, it overestimates early tumours. A 2-week delay after diagnostic biopsy is desired before completing an MRI. Alternatively, if logistics allow, a pre-biopsy MRI is preferred, especially for T1–T2 tumours.     

Biofilms and the tongue: therapeutical approaches for the control of halitosis

Due to its location and functions, the tongue is one of the most important anatomic structures in the oral cavity. However, knowledge in regards to its role and implications in oral health and disease is scarce. Moreover, although the dorsum of the tongue seems to harbour one of the most complex microbiological niches in human ecology, the knowledge of the role of tongue flora in health and disease is also very limited. Similarly, the nature of the tongue coating and the factors that influence its development and composition are almost unknown. The interest in the study of the tongue niche has increased in recent years due to its association with oral halitosis and to its role as a suitable reservoir for periodontal pathogens. The structure of the tongue favours a unique and complex bacterial biofilm, in which periodontal pathogens are frequently found. However, little is known about how to control this bacterial niche, and factors affecting tongue coating composition and aspect are not fully understood. Studies available on the influence of mechanical or antimicrobial approaches against tongue biofilm are very limited. Mechanical treatments showed a transient reduction in halitosis-related variables but were limited in time. Different antimicrobials agents have been evaluated: chlorhexidine, chlorine dioxide, metal ions, triclosan, formulations containing essential oils , and hydrogen peroxide. However, most studies were designed as short-term models. Some of these studies demonstrated that the reduction in halitosis-related variables was associated with significant changes in the tongue microflora.     

舌癌、舌乳头状瘤中细胞凋亡和细胞增殖及其与Bc1-2蛋白表达关系

目的 :探讨细胞凋亡和细胞增殖与舌鳞状细胞癌发生发展的关系。方法 :对 6 9例标本 ,其中正常舌粘膜 7例、舌乳头状瘤 2 0例 (维汉各 10 )、舌癌 42例 (高分化鳞癌 30例 ,中分化鳞癌 12例维汉各 2 1例 ) ,采用原位末端标记法和免疫组织化学染色技术并进行光镜下观察。结果 :不同民族和不同性别之间 ,凋亡指数 (TI)及增殖指数 (PI)无显著性差异 (P >0 .0 5 ) ;舌乳头状瘤组较正常组凋亡指数和增殖指数均增高 (P <0 .0 5 ) ;舌癌组中 ,随恶性程度的增加 ,增殖指数显著增高 ,凋亡指数降低 (P <0 .0 5 ) ;Bcl 2蛋白表达 ,自乳头状瘤→高分化鳞癌→中分化鳞癌而逐渐降低 (P <0 .0 5 ) ;乳头状瘤组 ,PI与TI呈正相关 (r =0 .6 13) ;舌癌组 ,PI与TI呈负相关 (r =-0 .6 0 1) ,Bcl 2蛋白表达与TI呈负相关 (r =-0 .484)。结论 :细胞凋亡和细胞增殖在肿瘤的发生发展中具有重要作用 ,bcl 2基因通过抑制细胞凋亡而使肿瘤细胞迅速聚积     

人舌鳞状细胞癌顺铂耐药细胞发生上皮-间质转化的研究

目的初步探讨人舌鳞状细胞癌（TSCC）化疗耐药与上皮．间质转化（EMT）的关系。方法以人舌鳞状细胞癌细胞、SCCl5及其顺铂耐药细胞株CAL27／CDDP、SCCl5／CDDP为研究对象。MTY检测两组亲本细胞及耐药细胞的半抑制率（IC50）,显微镜下观察两组亲本细胞及耐药细胞的形态．免疫荧光和Westonblot检测EMT相关分子标记蛋白E-cadherin和Vimentin的表达,Westonblot检测EMT转录因子Twistl和Slug的表达,Transwell及划痕实验检测细胞的侵袭迁移能力,结果采用单因素方差分析。结果CAL27／CDDP较CAL27IC。提高7．5倍（X^2=13．8043。P〈0．01）,SCCl5／CDDP较SCCl5IC50提高8．3倍（X^2=10．464,P〈0．01）。CAL27和SCCl5均为上皮细胞形态,CAL27／CDDP和SCCl5／CDDP呈间质细胞形态．且两组耐药细胞上皮标记蛋白E-cadherin表达下调．间质标记蛋白Vimentin表达上调,EMT转录因子Twistl、Slug表达上调,细胞的侵袭迁移能力明显增强。结论顺铂能成功诱导人舌鳞癌细胞发生EMT。     

HSV-TK/GCV联合IL-2对人舌癌移植瘤的抑制作用

目的观察HSV-TK联合IL-2对人舌癌细胞生长的抑制作用,并初步探讨其作用机理。方法建立舌癌Tca8113移植瘤动物模型,采用HSV-TK基因联合IL-2基因对人舌癌移植瘤进行裸鼠体内实验。实验分A、B、C、D四个实验组,每组5只裸鼠。A组为对照组,每瘤注射生理盐水;B组为空病毒组,每瘤注射空病毒5×108PFU;C组为单纯TK治疗组,每瘤注射TK5×108PFU;D组为TK联合IL-2治疗组,每瘤注射TK(5×108PFU) IL-2(5×108PFU)。48小时以后,对B、C、D组裸鼠每日腹腔注射GCV,100mg/kg/日,2次/日,连续10天。最后一次给药后第二天处死裸鼠,测量肿瘤重量并计算抑瘤率;光镜观察移植瘤组织学变化,透射电镜观察细胞的超微结构。结果A、B、C、D组裸鼠瘤重量均数分别为:1.52±0.49g,1.44±0.42g,0.91±0.24g,0.36±0.24g。C组的抑瘤率为40%;D组抑瘤率为76%。经统计学检验发现TK组和TK联合IL-2组的肿瘤生长均受到抑制,A组和C组、A组和D组之间有显著性差异(P<0.01)。与单用TK组比较,TK联合IL-2组抗瘤作用明显增强。光镜观察发现,TK组肿瘤组织呈灶状凋亡,可见核固缩的细胞。TK联合IL-2组凋亡细胞增加,肿瘤细胞减少。电镜观察发现,TK组和TK联合IL-2组出现大量的细胞凋亡:凋亡细胞皱缩,胞核体积缩小,染色质浓缩,电子密度增加,呈新月状边集于核膜下。结论TK联合IL-2治疗可显著抑制Tca8113移植瘤的生长,其治疗效果优于TK单独应用。