Shimadzu CL8000生化分析仪日常保养维护

目的:分析并整理常见的故障及维修方式。方法:总结多年来所见所闻仪器知识、维修经验,研究并分析常见的故障及维修方式。结果:介绍日常保养方法,以及吸样针、试剂针异常,冲洗针滴水,水空白异常,冰箱温度异常、定量泵损坏的原因,并提出解决方案。结论:在平时多掌握一些电学、设备学知识,使用设备的过程中做到每日清洁仪器表面,并定期保养仪器,对延长机器使用寿命,提高工作效率很有必要。     

瓦里安CL600C/D加速器磁控管灯丝及“MFIL”联锁故障的检修

本文简要介绍了磁控管的工作原理及磁控管灯丝的供电和联锁电路,以瓦里安CL600C/D加速器的磁控管灯丝供电及联锁电路为例,描述了几种常见灯丝联锁故障的检修方法。     

3例瓦里安CL23EX加速器故障联锁检修

本文介绍了瓦里安CL23EX加速器的系统结构和工作原理,以联锁故障为例,分析了实际工作中3个典型的瓦里安加速器故障,并总结了检修经验。     

CL战略在医院持续发展中的作用

我国医院在顾客导向方面先后走过了CI和CS阶段,目前正在进入第3个阶段即CL阶段.CL战略有助于医院保持持续发展的健康势头.其应用策略包括培育忠诚员工,借助虚拟人才打造品牌,建立差异化优势,实施感动服务和提高抱怨处理满意度等.     

从CI到CL看医院顾客导向的沿革

医院要在激烈的医疗市场竞争中站稳脚跟,必须实施顾客导向战略,即在医疗质量、品牌、服务等方面迎合顾客需求,满足顾客需要,这就是“顾客导向”。我国医院在走向市场化的过程     

瓦里安CL23EX医用直线加速器FLOW联锁故障维修1例

本文介绍了医用直线加速器加速管冷却管道堵塞的检查及处理方法。     

Single CX3CL1-Ig DNA administration enhances T cell priming in vivo

Upon antigenic stimulation, establishment of adaptive immune responses that determines vaccine efficacy is dependent on efficient T cell priming. Here, single CX3CL1-Ig DNA administration, a unique ligand of CX3CR1, together with viral or tumor antigens induced a strong in vivo antigen-specific T cell proliferation and effector function that was enough efficient to protect against a tumor challenge. We also showed that early expression of CX3CL1-Ig and antigens in muscle and lymphoid organs induces an increased in vivo migration of myeloid CD14+CD11c+ DC but not lymphoid CD8alpha+CD11c+ DC at these sites. Thus, by effectively directing DC toward lymphoid organs to encounter T cells, CX3CL1-Ig become a new candidate that augments T cell priming and increases efficiency of vaccination.     

日立7180与岛津CL8000全自动生化分析仪清洗效果对比

目的：将日立7180与岛津CL8000全自动生化分析仪进行对比研究,探讨两种分析仪的清洗效果,为临床获得准确的检验结果提供可靠依据。方法：抽取10名健康体检者静脉血各8 ml,平均分为4等份,分别在日立7180与岛津CL8000全自动生化分析仪上进行TG、TBA检测（A-D组）,从而判断两款全自动生化分析仪检测结果是否携带前次试剂,以及检测结束后的清洗效果。结果：A组TBA测量结果显著高于B组、C组及D组,对比结果差异具有统计学意义（x2=4.091,P＜0.05）;B组、C组及D组TBA测量结果对比差异无统计学意义。结论：岛津CL8000全自动生化分析仪清洗效果优于日立7180全自动生化分析仪,其性能稳定,清洗效果优越,可为临床医师提供准确诊断与治疗依据,值得推广使用。     

Spatial learning deficits induced by muscimol and CL218,872: lack of effect of prenatal malnutrition

The sensitivity of prenatal protein malnourished rats to the amnestic properties of the direct GABAA receptor agonist muscimol and the selective benzodiazepine (BZ) receptor agonist, CL218,872, was studied in the male offspring of rats provided with a protein deficient diet (6% casein) for 5 weeks prior to mating and throughout pregnancy. At postnatal day 90, rats were tested during acquisition of the submerged platform version of the Morris water maze task using four systemic doses of muscimol (0.1, 0.3, 1.0 and 1.8 mg/kg i.p.) or three systemic doses of CL218,872 (1.0, 3.2, and 5.6 mg/kg i.p.). In a dose dependent manner both drugs impaired acquisition of the task and impaired accuracy of the search pattern on the probe trial (platform removed). However, neither drug dissociated the performance of the two nutritional groups. These data are important in light of previous findings of differential behavioral effects of the non-specific BZ agonist, chlordiazepoxide (CDP), on spatial learning and on drug discrimination in prenatally malnourished rats and in the context of previous findings of reduced sensitivity to the anxiolytic effects of non-specific BZ receptor agonists across a wide variety of models of malnutrition. The present findings also support the concept that prenatal malnutrition does not affect the global functioning of the GABAA receptor, but fundamentally alters the way in which a subset of GABAA receptors (i.e. those containing the alpha2, alpha3 and/or the alpha5 but not the alpha1 subunit) is modulated by BZs.     

Spatial Learning Deficits Induced by Muscimol and CL218,872: Lack of Effect of Prenatal Malnutrition

Abstract

The sensitivity of prenatal protein malnourished rats to the amnestic properties of the direct GABA<PRE>A</PRE> receptor agonist muscimol and the selective benzodiazepine (BZ) receptor agonist, CL218,872, was studied in the male offspring of rats provided with a protein deficient diet (6% casein) for 5 weeks prior to mating and throughout pregnancy. At postnatal day 90, rats were tested during acquisition of the submerged platform version of the Morris water maze task using four systemic doses of muscimol (0.1, 0.3, 1.0 and 1.8 mg/kg i.p.) or three systemic doses of CL218,872 (1.0, 3.2, and 5.6 mg/kg i.p.). In a dose dependent manner both drugs impaired acquisition of the task and impaired accuracy of the search pattern on the probe trial (platform removed). However, neither drug dissociated the performance of the two nutritional groups. These data are important in light of previous findings of differential behavioral effects of the non-specific BZ agonist, chlordiazepoxide (CDP), on spatial learning and on drug discrimination in prenatally malnourished rats and in the context of previous findings of reduced sensitivity to the anxiolytic effects of non-specific BZ receptor agonists across a wide variety of models of malnutrition. The present findings also support the concept that prenatal malnutrition does not affect the global functioning of the GABA<PRE>A</PRE> receptor, but fundamentally alters the way in which a subset of GABA<PRE>A</PRE> receptors (i.e. those containing the 2, 3 and/or the 5 but not the 1 subunit) is modulated by BZs.     

瓦里安CL2300C／D型直线加速器低剂量率故障应急检修一例

本文根据瓦里安CL2300C/D型直线加速器低剂量率的故障现象结合随机所带的电原理图,详细地阐述了具体的检修过程和维修体会。     

Cytoprotective effect of polysaccharide isolated from different mushrooms against 7-ketocholesterol induced damage in mouse liver cell line (BNL CL. 2)

Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. Cytoprotective ability was evaluated by measuring the viable cells of the normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol. At 80 µg/mL of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% of viable cells after 20 h of incubation. With the addition of 200 µg/mL of each polysaccharide isolate to the cell line containing 80 µg/mL of 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus could significantly inhibit the 7-ketochoelsterol-induced cytotoxicity in the cells. But other polysaccharide isolates were not effective in inhibiting cell damage caused by the oxLDL-induced cytotoxicity.     

血红素氧合酶-1对牙髓炎模型妊娠期雌鼠干预效果及对趋化因子、前列环素表达的影响

目的研究血红素氧合酶-1 (HO-1)对牙髓炎模型妊娠期雌鼠干预效果及对趋化因子(CX3CL1)、前列环素(PGI2)表达的影响。方法 30只SD健康妊娠期雌鼠,20只建立牙髓炎模型,成功18只,分为模型组和HO-1组,各9只,另10只设为正常组。病理学观察采用酶联免疫吸附试验检测白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、血管内皮生长因子(VEGF)、CX3CL1、PGI2及HO-1水平。Western blot法检测Shh、Smo、Ptc及Gli1蛋白。结果与正常组相比,模型组和HO-1组IL-1β、TNF-α及VEGF水平升高(P<0. 05)。与模型组相比,血红素氧合酶组IL-1β、TNF-α及VEGF水平降低(P<0. 05)。与正常组相比,模型组和HO-1组CX3CL1、HO-1水平升高,PGI2水平降低(P<0. 05)。与模型组相比,血红素氧合酶组CX3CL1、HO-1水平降低,PGI2水平升高(P<0. 05)。与正常组相比,模型组和HO-1组Shh、Smo、Ptc及Gli1蛋白表达升高(P<0. 05)。与模型组相比,HO-1组Shh、Smo、Ptc及Gli1蛋白表达降低(P<0. 05)。结论 HO-1对牙髓炎模型妊娠期鼠起到抗炎效果,通过激活Sonic Hedgehog信号通路,改善CX3CL1、PGI2水平。     

不规则趋化因子在缺氧缺血性脑损伤新生大鼠脑组织中的表达及参附注射液干预作用的研究

目的:探讨新生大鼠缺氧缺血性脑损伤(HIBD)后大脑皮质不规则趋化因子(CX3CL1)活性的变化及参附注射液对其干预作用.方法:参照Rice法制备新生大鼠HIBD模型,7日龄SD新生大鼠随机分为假手术组(S组),生理盐水对照组(C组)和参附治疗组(SF组),每一组按照观察时间点的不同,进一步分为6h、12 h、1天、3天、5天、7天六个亚组,每个亚组8只.C组于造模成功后腹腔注射生理盐水10 ml/kg,1次/天,连用3天,SF组于造模成功后即刻腹腔注射等量参附注射液,S组仅分离右侧颈总动脉后腹腔注射等量生理盐水.免疫组化法和Western-Blot法检测大脑皮质CX3CL1的表达变化.结果:免疫组化法和Western-Blot法显示CX3CL1在S组各时间点表达量均较少,差异无统计学意义(P＞0.05);C组和SF组CX3CL1在各时间点的表达水平较S组明显升高,差异有统计学意义(P＜0.05),且在3天达到高峰,随后其表达下降;SF组CX3CL1在各个时间的表达水平均低于C组,差异有统计学意义(P＜0.05),但高于S组,差异有统计学意义(P＜0.05).结论:HIBD时CX3CL1表达增多,参与脑损伤炎症免疫反应,参附注射液能抑制CX3CL1大量表达从而抑制炎症免疫反应,对HIBD后的新生大鼠可能起到脑保护作用.