中医推拿疗法治疗小儿腹泻的临床研究

目的观察中医推拿疗法治疗小儿腹泻的临床疗效及其对免疫功能的影响。方法采用随机数字表法将80例腹泻患儿分为观察组和对照组,每组40例。对照组采用常规西医疗法,观察组在对照组基础上采用中医推拿疗法。比较2组患儿临床症状积分、免疫功能指标及总有效率。结果治疗后,2组患儿各项临床症状积分均较治疗前明显降低(P0.05),且观察组明显低于对照组(P0.05);观察组患儿体液免疫及细胞免疫功能指标(IgA、IgG、IgM、CD4~+、CD8~+、CD4~+/CD8~+)较治疗前及对照组明显改善(P0.05)。观察组总有效率明显高于对照组(P0.05)。结论采用中医推拿疗法治疗小儿腹泻临床疗效颇佳,能够改善患儿临床症状,调节机体免疫功能。     

仁术脐贴联合西医常规治疗小儿轮状病毒腹泻的临床观察

目的观察仁术脐贴联合西医常规治疗小儿轮状病毒腹泻的临床疗效。方法将162例腹泻患儿随机按照单盲法分为治疗组108例和对照组54例,两组患者均接受标准的西医常规治疗,治疗组在常规治疗上加用仁术脐贴。结果治疗组总有效率(93.3%)明显高于对照组总有效率(86.7%),两组差异有统计学意义(P0.05);治疗组平均治愈时间(4.5±1.2)d明显低于对照组平均治愈时间(6.0±1.8)d,两组差异亦有统计学意义(P0.05)。结论在西医常规治疗基础上联合仁术脐贴治疗小儿轮状病毒腹泻,可进一步提高疗效。     

思密达保留灌肠治疗小儿腹泻的临床分析

目的:观察思密达保留灌肠治疗小儿腹泻的疗效.方法:选择120例腹泻患儿随机分成两组.均给予常规治疗,治疗组在此基础上加用思密达保留灌肠.结果:治疗组总有效率为92.85%,对照组总有效率为72.85%.两组比较治疗组总有效率明显高于对照组(P＜0.05).结论:思密达保留灌肠治疗小儿腹泻疗效确切.     

推拿疗法治疗小儿腹泻疗效分析

目的探讨推拿疗法治疗小儿腹泻的效果。方法选取本院于2009年10月至2013年5月收治的420例小儿腹泻患者,随机分为对照组和观察组,各210例。对照组采用西医治疗,观察组在对照组的基础上给予推拿疗法,比较两组患儿疾病治疗效果情况。结果观察组患儿的治疗总有效率显著高于对照组患儿,两组结果对比,有显著的统计学意义(P0.01)。结论推拿治疗小儿腹泻效果确切,疗效显著。     

手穴推拿加脐穴艾灸、中药外敷治疗小儿迁延性、慢性腹泻临床观察

目的 通过临床观察,验证手穴推拿加脐穴艾灸、中药外敷治疗小儿迁延性、慢性腹泻的临床疗效,并对其作用机理进行深入探讨.方法 将本院迁廷性、慢性腹泻患儿共计193人,采用随机单盲法分组,观察组115例,对照组78例;观察组应用手穴推拿加脐穴(神阙)艾灸、中药外敷治疗;对照组口服枯草杆菌二联活菌颗粒(妈味爱),蒙脱石散(必奇)治疗.结果 治疗一个周期3天,治愈率:观察组75.04%,对照组43.65%;总有效率:观察组97.39%,对照组83.33%,两组治愈率、总有效率有极显著差异(P＜0.01).结论 应用手穴推拿加脐穴(神阙)艾灸、中药外敷治疗小儿迁延性、慢性腹泻,融多种中医外治法于一体,操作简单,安全可靠,疗效满意,患儿及家长易于接受.     

蒙托石散保留灌肠治疗小儿腹泻疗效观察

目的:观察蒙托石散保留灌肠治疗小儿腹泻的疗效。方法:随机将78例腹泻患儿分成对照组和治疗组,治疗组在常规治疗的基础上加用蒙托石散保留灌肠。结果:治疗组总有效率100%,对照组总有效率84.2%,两组比较治疗组总有效率明显高于对照组(P<0.05)。结论:蒙托石散保留灌肠疗效可靠,方法简便。     

喜炎平注射液联合葡萄糖酸锌治疗小儿迁延性腹泻的临床疗效

目的探讨喜炎平注射液联合葡萄糖酸锌治疗小儿迁延性腹泻的临床疗效及价值。方法选取我院2016年4月至2017年4月收治的64例小儿迁延性腹泻患儿,分为观察组与对照组。对照组患儿均采用常规对症治疗,观察组患儿在对照组基础上加用喜炎平注射液联合葡萄糖酸锌治疗。分析并对比两组临床疗效与复发情况。结果经不同药物治疗后,观察组治疗总有效率明显高于对照组,组间差异有统计学意义(P0.05);观察组患儿大便情况与复发次数明显优于对照组,组间差异有统计学意义(P0.05)。结论在对小儿迁延性腹泻进行临床治疗时,在常规对症治疗的基础上加用喜炎平注射液与葡萄糖酸锌联合治疗可有效改善患儿复发情况,治疗效果显著。     

推拿加拔罐治疗小儿外感咳嗽疗效观察

目的 探讨小儿外感咳嗽实施推拿加拔罐治疗的临床效果。方法收集144例外感咳嗽小儿患者,资料收集起止时间为2013年11月~2015年11月。按照入院顺序随机分组,分为对照组(71例)和观察组(73例),分别给予常规治疗和常规治疗联合推拿加拔罐治疗。治疗前后,分别对两组进行主要症状积分、次要症状积分评定,并进行治疗效果比较。结果两组患者治疗后的主要症状积分、次要症状积分与本组治疗前比较差异显著,均显著下降,且治疗后组间比较也存在明显差异,观察组积分均显著低于对照组(P0.05)。经疗效评估,观察组的总有效率为93.15%,总有效率显著高于对照组(P0.05)。结论小儿外感咳嗽在常规治疗的基础上联合实施推拿加拔罐治疗效果确切,可有效改善患儿的临床症状,提高有效率。     

中西医结合治疗小儿反复呼吸道感染临床观察

目的观察中西医结合治疗小儿反复呼吸道感染的临床疗效。方法将2006年10月—2010年10月收治的小儿反复呼吸道感染患儿116例,随机分为治疗组60例和对照组56例。对照组予西医常规治疗,治疗组在对照组治疗的基础上加服中药(自拟)扶正防感合剂。结果治疗组总有效率明显高于对照组,差异有统计学意义(P<0.01)。结论中西医结合治疗小儿反复呼吸道感染疗效优于单纯西医常规治疗。     

痰热清注射液联合西医常规治疗慢性阻塞性肺疾病急性加重期临床观察

目的观察痰热清注射液联合西医常规治疗慢性阻塞性肺疾病急性加重期(AECOPD)的临床疗效。方法将70例AECOPD患者随机分为两组,对照组采用西医常规治疗,治疗组在对照组治疗基础上加用痰热清注射液。结果治疗组总有效率明显高于对照组(P0.05)。治疗组治疗后氧分压(Pa O2)明显提高,血气二氧化碳分压(Pa CO2)明显下降(P0.05)。结论痰热清注射液联合西医常规治疗疗效显著,可以有效改善患者血气指标,提高临床治疗效率和术后生活质量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.