食管鳞癌中有活性的激酶C受体表达与临床病理参数的关系

目的 研究有活性的激酶C受体(RACK1)在食管鳞癌组织中的表达水平及其临床意义.方法 Western印迹检测食管鳞癌细胞中RACKI的表达.免疫组织化学方法检测组织芯片中113例食管鳞癌组织及其配对的癌旁正常食管黏膜中RACKl和Ki67蛋白的表达水平.将病例按年龄、性别、肿瘤部位、吸烟与否、分化程度和TNM分期进行分组,比较不同组别中RACKl表达水平的差异.结果 RACKl在食管鳞癌组织中的表达低于癌旁正常食管黏膜,差异具有统计学意义(x2=63.363,P<0.01).不吸烟组的肿瘤组织中RACKl高表达率为72.5%(29/40),高于吸烟组的46.6%(34/73)(X2=7.040,P=0.008).TNM l期和Ⅱ期患者的肿瘤组织中RACKl高表达率为63.8%(44/69),高于TNMⅢ期的43.2%(19/44)(0=4.616,P=0.032).Ki67评分越高的肿瘤组织中RACKl的表达越低(x2=8.261,P=0.016).结论 RACKl在食管鳞癌中表达发生下调.食管鳞癌中RACKI的表达与吸烟、TNM分期及Ki67评分具有相关性.

Abstract：

Objective To explore the expression level and clinical significance of receptor for activated C kinase 1(RACKl)in esophageal squamous cell carcinoma(ESCC).Methods Western Blotting was performed to detect the RACKl expression in ESCC cell lines.Immunohistochemistry was conducted to assay the expression of RACKl and Ki67 in tumor tissues and adjacent normal epithelium from 113 ESCC patients in tissue microarray.The relationship between the RACKI level and such clinieopathologic profiles as age,gender,location,smoking,differentiation degree and TNM(tumor,node,metastasis)stage were analyzed.Results The expression of RACKl protein was significantly downregulated in ESCC tissues as compared with the normal adjacent epithelium(X2=63.363,P<0.01).An upregulated expression of RACKl was observed in 72.5%(29/40)ESCC tissues of patients without a smoking history.And it was significantly higher than that in 46.6%(34/73)of patients with a smoking history(x2=7.040,P=0.008).In addition,the rate of up-regulated of RACKl was significantly higher in stage I andⅡgroup(63.8%,44/69)than that in stage Ⅲ group(43.2%,19/44)(x2=4.616,P=0.032).Moreover.the ESCC tissues with a higher Ki67 score showed a lower level of RACKl than that with a lower Ki67 score(x2=8.261,P=0.016).Conclusions The expression of RACKl is down-regulated in ESCC tissues and it is associated with smoking,The expression of RACKl was associated with smoking,TNM staging and Ki67 score of ESCC.     

Notch1在食管鳞癌中的表达及其与毛细血管新生的关系

目的 研究Notch1在食管鳞癌中的表达与临床病理特征的关系及其与食管鳞癌新生毛细血管的关系.方法 通过免疫组化方法检测40例食管鳞癌和8例正常食管组织的Notch1和血管内皮生长因子(VEGF)、毛细血管密度(MVD)的表达情况并行相关性分析.结果 Notch1在食管鳞癌组织中表达明显低于正常对照组(P<0.05),在不同的分化程度中有差异(P<0.05),但在肿瘤浸润深度和淋巴结转移与否中无明显差异(P>0.05).VEGF和MVD在食管鳞癌组织中表达明显高于正常对照组(P<0.05),在不同分化程度、浸润深度和淋巴结转移与否中各组间均有显著性差异(P<0.05).相关性分析显示Notch1与VEGF为负相关关系.结论 Notch1在食管鳞癌中可能为抑癌基因;在早中期食管鳞癌中可能是主要影响肿瘤细胞的分化;其的异常低表达可能是引起VEGF和MVD异常高表达的原因之一.Notch信号通路在食管鳞癌新生毛细血管中可能有关键作用.

Abstract：

Objective To observe Notch1 expression in esophageal squamous cell carcinoma (ESCC) and investigate its relation with microvascular angiogenesis in the tumor. Methods Tissue slices of 40 cases ESCC (cancer group) and 8 cases normal esophagus tissues (normal group) were obtained to analyze the expression of Notch 1 and vascular endothelial growth factor (VEGF) using immunohistochemistry and estimate the micro vessel density (MVD) in the tumor. Results Notch 1 expression was significantly lower in the cancer group than in the normal group (P<0.05). In the cancer group, Notch 1 expression was higher in highly differentiated than in poorly differentiated tumors (P<0.05) regardless of tumor infiltration or lymph nodes metastasis (P>0.05). VEGF expression and MVD were significantly higher in cancer group than in normal group, and showed significant differences between tumors with different differentiation degrees, infiltration and lymph node metastasis (P<0.05). Correlation analysis showed that Notchl expression was inversely correlated to VEGF expression. Conclusion Notch1 may be an anti-oncogene in ESCC and affects cell differentiation in early stage of the malignancy. Abnormally low expression of Notchl in ESCC may be one of the upstream factors to induce high expression of VEGF and increased MVD. The Notchl pathway might play a key role in microvascular angiogenesis in ESCC.     

食管鳞癌组织中缺氧诱导因子-1α和6-氧-甲基鸟嘌呤-DNA甲基转移酶蛋白的表达与预后的关系

目的 研究食管鳞状细胞癌(ESCC)中缺氧诱导因子-1α(HIF-1α)、6-氧-甲基鸟嘌呤-DNA甲基转移酶(MGMT)蛋白的表达及其与食管癌病理特征的关系.方法 采用免疫组织化学(SP)法检测58例食管鳞癌组织中HIF-1α、MGMT蛋白的表达,并分析其与临床病理各因素之间的关系.结果 58例患者的ESCC组织中HIF-1α、MGMT蛋白均有表达,阳性表达率分别为51.4%、39.6%,MGMT 蛋白的表达与食管鳞癌的分化程度密切相关(P<0.05),而与性别、年龄、分期及淋巴结转移无关.HIF-1α蛋白的表达与性别、年龄、分期、分化程度以及淋巴结转移均无关(P均>0.05).HIF-1α与MGMT 蛋白表达无明显相关关系(P>0.05).MGMT阳性表达患者生存时间(OS)较阴性组明显延长(P<0.05),而HIF-1α蛋白表达与OS无明显相关(P>0.05).结论 HIF-1α蛋白在食管鳞癌组织中呈高表达,MGMT与其分化程度相关,两者检测对判断预后有重要意义. Abstract： Objective To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and O~6-methylguanine DNA methytransferase(MGMT) in esophageal squamous cell carcinoma(ESCC) tissue and its relation with the clinicopathalogy features. Methods The expression of HIF-1α, MGMT in 58 cases of ESCC were detected by immnohistochemical staining. Results The positive rates of HIF-1α,MGMT in ESCC tissue were 51.4%,39.6%, respectively. The positive expression rates of HIF-1α and MGMT were not correlated with the degree of sex,age,clinical stage and differentiation(P>0.05), but MGMT expression was correlated with differentiation and overall survival time (P<0.05). The expression of HIF-1α was not correlated with that of MGMT (P>0.05), and HIF-1α was not correlated with overall survival time. Conclusions HIF-1α, MGMT play important roles in esophageal carcinoma,MGMT was correlated with the differentiation of esophageal carcinoma, respectively, which may be used as important indexes to evaluate the prognosis of esophageal carcinoma.     

抑癌基因WWOX在食管鳞状细胞癌中的表达及意义

目的 探讨食管鳞状细胞癌(ESCC)组织中WWOX mRNA和蛋白的表达及其意义.方法 应用RT-PCR和免疫组化SP法检测50例ESCC组织、19例癌旁不典型增生组织及50例正常食管黏膜组织中WWOXmRNA和蛋白的表达.结果 WWOX在癌组织、癌旁不典型增生组织及正常黏膜组织中mRNA的相对表达量分别为0.81±0.08、0.84±0.05和0.90±0.05,组间比较差异有统计学意义(F=45.736,P<0.05);不同分化程度、TNM分期、浸润深度及有无淋巴结转移的ESCC组织之间WWOX mRNA相对表达量差异均有统计学意义(P均<0.05).ESCC组织及癌旁不典型增生组织中WWOX蛋白表达均低于正常黏膜组织,蛋白阳性表达率分别为42.0%(21/50)、64.8%(13/19)和84.0%(42/50),组间比较差异有统计学意义(x2=20.002,P<0.01);ESCC组织中WWOX蛋白表达与癌组织的分化程度、TNM分期、浸润深度及有无淋巴结转移密切相关(P<0.05).结论 ESCC组织中WWOX mRNA和蛋白表达均降低,其低表达可能与ESCC发生发展有关.检测WWOX mRNA及蛋白的表达可望成为食管鳞癌早期诊断和预后的分子指标之一. Abstract： Objective To explore the expression of WWOX mRNA and protein inesophageal squamous cell carcinoma (ESCC) tisuss. Methods The expression of WWOX was detected by RTPCR and SP immunohistochemical methods in 50 cases of ESCC tissue, 19 cases of mucosa adjacent to cancer, and 50 cases of normal esophageal mucosa. Results The relative content of WWOX mRNA depressed by turns in cancer tissue, mucosa adjacent to cancer and normal mucosa tissue which were 0.81 ± 0.08,0.84 ± 0.05,0.90 ± 0.05, respectively. And there was significant difference in group comparison (F = 45. 736, P < 0.05). The expression of WWOX mRNA was closely correlated with the tumor grade, TNM grade, infiltrative depth and lymphatic metastasis in ESCC tissue (P < 0.05). The positive rate of WWOX protein expression in cancer tissue and mucosa adjacent to cancer were lower than that of normal mucosa tissue which were 42.0% (21/50), 64.8 % (13/19), 84.0% (42/50) respectively. And there was a significant difference in group comparison(P < 0.05). The expression of WWOX protein was closely correlated with the tumor grade, TNM grade,infiltrative depth and lymphatic metastasis in ESCC tiissue (P <0.05). Conclusions The expression of WWOX mRNA and protein decreased in ESCC tissue, which suggest the WWOX gene may be related to oncogenesis of ESCC tissue. Combined detection of the expression of WWOX mRNA and protein may be used in early diagnosis and prognosis judgement of ESCC tissue.     

食管鳞癌组织中14-3-3ε表达与临床病理因素及预后的相关性

目的 研究14-3-3ε在食管鳞癌及正常食管黏膜中的表达及其临床意义.方法 免疫组化检测72例食管鳞癌及其配对癌旁正常食管黏膜的14-3-3ε表达水平.运用X2检验分析肿瘤组织14-3-3ε表达与临床病理因素的关系.运用Kaplan-Meier法分析肿瘤组织14-3-3ε与患者术后总生存时间的关系.结果 14-3-3ε在食管鳞癌中阳性率和强阳性率分别为77.8%和27.8%;在正常食管黏膜中表达阳性率和强阳性率分别为43.1%和2.8%,其肿瘤高表达差异有统计学意义(P＜0.01).在56例14-3-3ε阳性的肿瘤中,37例(66.1%)为细胞质表达,4(7.1%)例为核表达,15例(26.8%)为质核共表达;在31例14-3-3ε阳性的正常食管黏膜中,无细胞质表达,13例(41.9%)为细胞核表达,18例(58.1%)为质核共表达.该蛋白在肿瘤细胞与正常细胞的定位差异有统计学意义(P＜0.01).通过与临床病理因素的进一步分析,发现肿瘤细胞14-3-3ε表达强阳性与淋巴结转移(P=0.028)和术后5年生存率相关(P=0.018).生存曲线提示肿瘤14-3-3ε强阳性患者预后不良(Log-rank,P=0.031).14-3-3e定位差异与各临床病理因素及术后生存无显著相关性.结论 14-3-3ε在食管癌中表达上调;该蛋白在肿瘤中多表达于细胞质,在正常食管黏膜中多表达于细胞核;14-3-3ε表达水平与淋巴结转移和预后不良呈正相关.

Abstract：

.Objective To investigate the expression of 14-3-3ε in esophageal squamous cell carcinoma (ESCC) and relationship of 14-3-3ε and clinicopathological factors of ECSS patients. Methods The 14-3-3ε expression in tumors and adjacent normal epithelia from 72 ESCC patients was detected by immunohistochemical staining. Correlations of 14-3-3ε expression or their subcellular localization and clinicopathological factors were analyzed using Chi-squared test. Survival curves were generated according to the Kaplan-Meier method, and the statistical analysis was performed by Log-rank test. Results The expression of 14-3-3ε protein was significantly up-regulated in tumors with 77. 8% positive and 27. 8%strong positive staining, compared with paired adjacent normal epithelia with 43. 1% positive and only 2. 8%strong positive staining (P ＜ 0. 01 ). In a total of 56 positive staining tumors, 14-3-3ε was detected in cytoplasm of 37 (66. 1% ), in nucleus of 4 (7. 1% ) ,in both cytoplasm and nucleus of 15 (26. 8% ) cases.Whereas, in a total of 31 positive staining normal epithelia, 14-3-3ε was detected in cytoplasm of 0, in nucleus of 13 (41.9%), in both cytoplasm and nucleus of 18 ( 58. 1% ) cases ( P ＜ 0. 01 ). Statistical analysis revealed that strong 14-3-3ε expression was correlated with lymph node metastasis (P = 0. 028) and lower 5-year survival (P = 0. 018). The survival curves calculated by Kaplan-Meier method further showed that the patients with strong 14-3-3ε expression had a shorter survival than patients with negative or weak 14-3-3ε expression (Log-rank,P =0. 031 ). No correlation was found between subcellular localization of 14-3-3ε in tumor cells and clinicopathologic factors and prognosis of ESCC patients. Conclusion The 14-3-3εexpression is significantly up-regulated in ESCCs. It was usually located in cytoplasm of tumor cells, but nucleus of normal epithelia. Strong expression of 14-3-3ε in tumors is associated with lymph node metastasis and considered as an poor prognostic factor for ESCC.     

Expression of p57kip2 and cyclinE proteins in human pancreatic cancer

Objective To investigate the effects of p57kip2 and cyclinE proteins on the genesis and progression of human pancreatic cancer. Methods The expression of p57kip2 and cyclinE proteins in tumor tissues and adjacent tissues of pancreatic cancer in 32 patients was detected by SP immunohistochemical technique. Results The p57kip2 protein positive-expression rate in tumor tissues of pancreatic cancer was 46.9%, which was lower than that in adjacent pancreatic tissue (P&lt;0.05). The p57kip2 protein positive-expression correlated significantly with tumor cell differentiation (P&lt;0.05) and did not correlate significantly with lymph node metastasis (P&gt;0.05). The cyclinE positive-expression rate in tumor tissues was 68.8%, which was higher than that in adjacent pancreatic tissues (P&lt;0.05). The cyclinE positive-expression also correlated significantly with tumor cell differentiation and lymph node metastasis (P&lt;0.05). The cyclinE protein positive-expression rate in the tumor tissues of the p57kip2 protein positive-expression group was lower than that in the p57kip2 protein negative-expression group, and there were no significant correlation between the two groups (r= -0.112, P＞0.05).Conclusion Decreased expression of the p57kip2 protein and/or over-expression of the cyclinE protein may play an important role in the genesis and progression of human pancreatic cancer.     

Vasculogenic mimicry and aberrant expression of HIF-lα/E-cad are associated with worse prognosis of esophageal squamous cell carcinoma

This study aims to find good markers for predicting the prognosis of patients with esophageal squamous cell carcinoma (ESCC). Vasculogenic mimicry (VM) and the expression of hypoxia inducible factor-lα (HIF-lα)/E-cad protein in ESCC were investigated by immunostaining. The association between VM, HIF-lα/E-cad and clinicopathologic characteristics and 5-year-survival rate of patients with ESCC was analyzed. A total of 160 ESCC specimens were involved in this study and 28 specimens of normal esophageal mucosa served as controls. VM channels were identified in 78 (48.75%) of the 160 ESCC specimens and none of the normal esophageal mucosa was found to have VM. The rates of high-expression of HIF-lα and E-cad in ESCC were 43.75% and 38.75%, while the rates in control were 17.86% and 71.43%, respectively (P<0.05 for all). VM and the expression levels of HIF-lα and E-cad were significantly related to lymph node metastasis, serosa infiltration, PTNM staging and 5-year-survival rates of patients with ESCC (P<0.05 for all). VM was positively correlated with HIF-lα but negatively with E-cad, and HIF-lα was negatively correlated with E-cad (P<0.001 for all). The 5-year-survival rate of patients with ESCC was 6.41% (5/78) in VM group and 65% (52/82) in non-VM group, 7.14% (5/70) in high HIF-lα expression group and 57.78% (52/90) in low HIF-lα expression group. Oppositely, the 5-year-survival rate in high E-cad expression group was 80.65% (50/62) and that in low E-cad expression group was 7.37% (7/98) (P<0.05 for all). Cox multifactor regression analysis indicated that lymph node metastasis, PTNM stage, VM and expression levels of HIF-lα and E-cad were independent risk factors of patients with ESCC (P<0.05 for all). Combined detection of VM, HIF-lα and E-cad plays an important role in predicting the invasion, metastasis and prognosis of patients with ESCC.     

PIN1 Gene Overexpression and β-catenin Gene Mutation/Expression in Hepatocellular Carcinoma and Their Significance

The evolution of hepatocellular carcinoma (HCC) is a compound process which involves many kinds of genes and transductional pathways. The expression of the peptidyl-proplyl- isomerase PIN1 gene, the mutation in exon 3 of β-catenin and its correspondent abnormal expression and their roles in the hepatocellular carcinogeneisis were investigated. Among 29 pair cases of HCC and non-carcinoma tissues, the expression of PIN1 gene was detected by immunochemical staining. Mu-tations in exon 3 of β-catenin gene and differential expression of β-catenin gene were investigated by the methods of PCR-SSCP, direct sequencing and immunohistochemical technique as well. The re-sults indicated: (1) 44.8% (13/29) cases of HCC presented higher level of PIN1 gene expression than non-cancerous tissues (χ2 =32.63, P<0.05), especially in cytoplasm and nucleus, while there was lower level of PIN1 expression in non-cancerous tissues; (2) 58.6% (17/29) HCC tissues showed β-catenin protein accumulation in cytoplasm and nucleus. 46.2% (6/13) HCC tissues indicated β-catenin protein accumulation with higher level of PIN1 expression, while 53.8% (7/13) HCC tis-sues indicated β-catenin protein accumulation with lower level or trace of PIN1 expression (χ2 =0.00, P>0.05); (3) 24.1% (7/29) of primary tumor lesions carried gene mutations in exon 3 of β-catenin, and accompanied by β-catenin protein accumulation. There was no mutation in non-cancerous tissues. All the mutation presented in tissues with low level of PIN1 expression. There was no mutation of β-catenin gene in tissues with high PIN1 expression level (χ2=58.12, P<0.05). So it was postulated that the increase of PIN1 gene expression could promote hepatocellular carcinogenesis via a way dif-ferent from β- catenin gene mutation.     

应用组织芯片技术研究人类胰腺癌中前列腺干细胞抗原的表达

目的 检测PSCA在胰腺癌中的表达情况,探讨PSCA在胰腺癌发病中所起的作用.方法 用组织芯片技术构建包含78例导管腺癌,12例慢性胰腺炎患者,10例正常胰腺组织的100点阵的石蜡组织芯片.用免疫组化SP法检测该芯片中PSCA的表达,分析其与胰腺癌I临床病理因素的关系.结果 78例胰腺癌患者中,PSCA阳性表达率为79.5%(62/78),与正常组比较PSCA表达与胰腺癌显著相关(X~2=15.81,P<0.005),与慢性胰腺炎比较PSCA亦与胰腺癌显著相关(X~2=11.33,P<0.005);PSCA表达与年龄、性别、组织分化程度及TNM分期无明显相关性.结论 PSCA阳性表达与胰腺癌相关,可能与胰腺癌的发生发展有着密切关系,但与胰腺癌的临床病理特型无关.

Abstract：

Objective To investigate the expression of prostate stem cell antigen (PSCA) in human pancreatic carcinoma and explore its role in the oncogenesis of pancreatic cancer. Methods A pancreatic carcinoma tissue microarray was constructed, which contained 10 normal adult pancreas tissues, 12 chronic pancreatitis tissues and 78 pancreatic carcinomas. Immunohistochemistry was employed to detect the expression of PSCA, and the relation between PSCA expression and the clinicopathological factors of pancreatic carcinoma was analyzed. Results The positivity rate of PSCA in pancreatic carcinoma was 79.5 % (62/78), and PSCA staining was more intense in the malignant cells than in the benign cells (x~2=15.81, P<0.005) and chronic pancreatitis tissues (x~2=11.33, P<0.005). No obvious association was found between PSCA expression and the other variables of pancreatic carcinoma (including gender, age at surgery, tumor grade, and TNM stages). Conclusion The expression of PSCA can be related to the development of pancreatic cancer, but not to the clinicopathological factors of the tumor.     

FAM135B与赖氨酸乙酰转移酶在维吾尔族食管鳞状细胞癌中的表达

目的探讨序列相似家族135成员B（FAM135B）与赖氨酸乙酰转移酶（KAT5）在维吾尔族食管鳞状细胞癌（ESCC）中的表 达规律。方法使用罗氏全自动免疫组化仪检测40对维吾尔族ESCC及其癌旁组织中FAM135B与KAT5的表达情况,分析两 种蛋白间表达的相关性及与临床特征的相关性。结果维吾尔族ESCC标本中FAM135B、KAT5 表达分别占92.50%（37/40）、 15.00%（6/40）;癌组织中FAM135B 表达强阳性者所占比例高于癌旁组织［45.00%（18/40）vs 22.50%（9/40）,χ 2=4.528,P= 0.033］;癌组织中KAT5 表达阴性者所占比例与癌旁组织差异无统计学意义［85.00%（34/40）vs 87.50%（35/40）,χ 2=0.105,P= 0.745］;ESCC 与其配对癌旁组织FAM135B 强阳性表达具有良好正相关性（Kendall 相关系数=0.707,P<0.001）;癌组织的 FAM135B强阳性表达与其KAT5表达具有显著负相关性（Kendall相关系数=-0.946,P<0.001）;FAM135B与KAT5表达与ESCC 患者性别、年龄、肿瘤部位、分化程度、浸润深度、淋巴结转移及临床分期均无明显相关性（P>0.05）。结论FAM135B强阳性表 达可能是维吾尔族ESCC发生的重要分子基础,且该分子可能通过KAT5的负性表达发挥作用。     

食管鳞状细胞癌组织中LRIG3和EGFR蛋白的表达

目的:探讨LRIG3和EGFR蛋白在食管鳞状细胞癌中的表达及其意义。方法:采用免疫组织化学SP法检测60例食管鳞状细胞癌、60例食管正常组织中LRIG3及EGFR的表达水平。结果:LRIG3蛋白在食管鳞状细胞癌组织中的阳性表达率低于食管正常组织(χ2=60.480,P<0.001),EGFR蛋白在食管鳞状细胞癌组织中的阳性表达率高于食管正常组织(χ2=32.976,P<0.05)。LRIG3和EGFR的表达与食管鳞状细胞癌的分化程度、淋巴结转移、肿瘤分期有关(χ2=3.939和4.318、4.374和5.910、5.293和5.884,P<0.05),与性别、年龄无关。LRIG3和EGFR蛋白在食管鳞状细胞癌组织中的表达呈负关联(rP=0.264,P=0.034)。结论:LRIG3表达下降和EGFR表达升高可能在食管鳞状细胞癌的发生发展中起重要作用。     

食管鳞癌中Survivin和Smac/Diablo的表达及意义

目的:探讨Survivin和Smac/Diablo在食管鳞癌中的表达及其临床意义。方法:应用免疫组化SP法对80例经病理确诊为食管鳞癌的病例和相对应的30例病理确定为正常组织及30例癌旁组织进行Survivin蛋白和Smac/Diablo蛋白半定量免疫组化分析。结果:Survivin在食管鳞癌组织,癌旁组织,正常组织的阳性表达率分别为81.25%,10.0%,3.33%,有显著性差异(χ2=72.983,P<0.01)。Smac/Diablo在食管鳞癌组织,癌旁组织,正常组织的阳性表达率分别为61.25%,76.67%,93.33%,有显著性差异(χ2=11.518,P<0.01)。Survivin和Smac/Diablo表达与患者的年龄、性别、分化程度、浸润深度等均无明显关系(P>0.05)。但与TNM分期、淋巴结转移相关(P<0.05)。在Smac阳性组中的表达率为23.21%(13/56),在Smac/Diablo阴性组中的表达率为16.67%(9/54),两者间有统计学差异(P<0.05),两者关联性较强,呈负相关。Survivin阳性率与淋巴结转移呈低度正相关(r=0.329,P<0.05),Smac/Diablo阳性率与淋巴结转移呈负相关(r=-0.733,P<0.05)。结论:Survivin蛋白和Smac/Diablo蛋白表达与食管癌的病理分期,淋巴结转移都有着密切的关系,Smac/Diablo在食管鳞癌组织中低表达,其表达和Survivin呈明显的负相关,两者可能在食管鳞癌细胞凋亡信号传导网络中发挥重要作用。     

食管鳞状细胞癌DNA结合抑制蛋白-1的表达及临床意义

目的 探讨食管鳞状细胞癌（ESCC）中DNA结合抑制蛋白-1（ID-1）的表达与ESCC细胞分化程度、淋巴结转移及患者的年龄、性别的关系。方法 免疫组织化学方法检测118例ESCC患者手术切除标本及其20例癌灶周围正常组织ID-1的表达。结果 ID-1表达于肿瘤间质和肿瘤细胞的细胞质中。102例患者不同程度表达ID-1（86．44％），而13例癌旁正常组织低表达（65％），两者比较差别有统计学意义（P〈0．01）。高分化鳞癌表达明显高于低分化鳞癌（P〈0．01）。38例伴有淋巴结转移的肿瘤原发灶者30例（78．95％）表达ID-1，80例无淋巴结转移者72例表达ID-1（90％），两者比较无统计学意义（P〉0．05）。ESCC中ID-1的表达与患者的性别及年龄没有相关性（P〉0．05）。结论ID-1在ESCC中的表达明显高于正常组织，其表达强度与食管癌分化程度呈正相关。与年龄、性别及淋巴结转移无关。     

Shh与MMP2在人食管鳞癌组织中的表达及意义

目的探讨音猬因子（Shh）与基质金属蛋白酶2（MMP2）在人食管鳞癌组织中的表达以及与临床病理参数之间的关系。方
法采用免疫组化SP法检测Shh与MMP2在48例人食管鳞癌组织以及44例癌旁组织中的表达情况。结果Shh的阳性表达主
要定位在细胞质和胞核,在食管鳞癌组织中的阳性表达率为75%明显高于癌旁组织（20.45%）,且与患者的TNM分期有关;而
MMP2的阳性表达主要分布在胞质,其在食管鳞癌组织中的阳性表达率为68.75%,明显高于癌旁组织中的22.72%,与淋巴结转
移与否,TNM分期有关,且均具有统计学意义（P<0.01）。48例人食管鳞癌组织中Shh蛋白与MMP2蛋白的表达呈正相关（R=
0.037,P=0.019）。结论Shh蛋白的表达可能参与到食管鳞癌的侵袭与转移。
     

胰岛素样生长因子-1受体在食管鳞状细胞癌的表达及意义

目的 探讨胰岛素样生长因子-1受体(IGF-1R)在食管鳞状细胞癌(ESCC)中的表达及意义. 方法 免疫组织化学方法检测118例ESCC手术切除标本及20例癌旁正常组织中IGF-1R、Ki-67及Bcl-2的表达情况. 结果 ESCC中IGF-1R、Ki-67及Bcl-2呈高表达,阳性率分别为80.51%、81.36%和59.32%;IGF-1R及Ki-67表达程度与肿瘤组织分化程度负相关;Bcl-2表达程度与肿瘤组织分化程度正相关;IGF-1R表达与是否伴淋巴结转移有关;且IGF-1R表达与Ki-67及Bcl-2表达呈正相关. 结论 调控细胞增殖及凋亡可能是IGF-1R参与ESCC发生发展的主要机制,并可用于评价ESCC是否伴有淋巴结转移.     

32例食道鳞癌MDR1及MRP基因表达结果分析

目的探讨多药耐药(MDR1)基因、多药耐药相关蛋白(MRP)基因在食道鳞癌中的表达情况及其与肿瘤分化程度的关系.方法用逆转录多聚酶链反应(RT-PCR)方法检测新鲜食道癌组织标本.结果32例食道鳞癌标本MDR1、MRP基因阳性表达率分别为78.1%、75.0%,明显高于癌旁组织(均P＜0.01).MDR1、MRP基因表达与肿瘤分化程度无关(均P＞0.05).结论MDR1、MRP基因在食道鳞癌组织中有较高的阳性表达率,但与食道鳞癌的组织分化程度无关.     

食管鳞癌组织中抑癌基因Crnn的表达及其临床意义

目的:研究Crnn蛋白在食管鳞癌组织(ESCC)中的表达及其在ESCC发生、发展及预后中的作用。方法:采用免疫组织化学方法检测组织阵列仪取样的100例ESCC组织(观察组)和相应正常食管组织(对照组)中Crnn蛋白的表达情况,并探讨其与ESCC患者年龄、性别、肿瘤细胞分化程度、淋巴结转移、TNM分期、大体分型和5年生存率的关系。结果:ESCC组织中Crnn蛋白表达阳性率(45.6%)明显低于正常组织中的表达阳性率(93.7%)(P<0.05);肿瘤组织Crnn表达下调率为53.2%;Crnn基因表达与淋巴结转移、TNM分期有关,而与年龄、性别、肿瘤细胞分化程度、和大体分型无关;Crnn蛋白表达下调的食管鳞癌患者的5年生存率明显低于Crnn蛋白正常表达的食管鳞癌患者。结论:Crnn基因表达下调可能会促进食管鳞癌的发生、发展,是食管鳞癌组织中候选的抑癌基因。