日本血吸虫感染可诱导共刺激分子配体基因敲除小鼠CD154和CD40动态变化及对Th1/Th2偏移的影响

目的:探讨可诱导共刺激分子( inducible costimulator,ICOS)-ICOS配体( ICOS ligand,ICOSL)信号通路对感染日本血吸虫小鼠的CD154、CD40表达及Th1/Th2偏移的影响. 方法:建立ICOSL基因敲除( ICOSL knockout, ICOSL-KO)小鼠及野生型C57BL/6J小鼠感染日本血吸虫疾病模型,于感染前和感染后4、7、12、16、20周,应用流式细胞术、免疫组织化学法分别检测小鼠脾细胞与肝虫卵肉芽肿周围炎性浸润细胞CD154、CD40的水平;应用酶联免疫吸附测定法( enzyme-linked immunosorbent assay,ELISA)检测小鼠脾细胞经可溶性虫卵抗原( soluble egg anti-gen,SEA)诱导72 h后培养的上清液中γ-干扰素(interferon gamma,IFN-γ)、白细胞介素-4(interleukin-4,IL-4)水平及小鼠血清中SEA特异性抗体IgG、IgG1、IgG2a的水平;应用HE染色观察小鼠肝虫卵肉芽肿病变动态变化. 结果:感染后4、7、12、16、20周,ICOSL-KO小鼠脾细胞CD154、CD40 水平与野生型小鼠相比,显著降低 [ CD154 为(18. 62 ± 4. 76)% vs. (27. 91 ± 3. 94)%、(22. 44 ± 4. 67)% vs. (40. 86 ± 5. 21)%、(25. 50 ± 6. 81)% vs. (43. 81 ± 8. 41)%、(20. 22 ± 5. 28)% vs. (40. 95 ± 7. 34)%、(17. 87 ± 4. 59)% vs. (33. 16 ± 6. 31)%,皆P<0. 01;CD40为(19. 43 ± 3. 26)% vs. (24. 37 ± 3. 59)%、(23. 00 ± 4. 47)% vs. (31. 80 ± 5. 86)%、(24. 46 ± 5. 01)% vs. (35. 85 ± 5. 32)%、(23. 42 ± 4. 69)% vs. (33. 30 ± 6. 14)%、(22. 85 ± 3. 78)% vs. (30. 88 ± 5. 94)%,皆P<0. 05 ]. 感染后7、12、16、20周,ICOSL-KO小鼠肝虫卵肉芽肿炎性浸润细胞 CD154、CD40 水平与野生型小鼠相比,亦显著降低[CD154为(0. 319 ± 0. 066) vs. (0. 488 ± 0. 086)、(0. 389 ± 0. 067) vs. (0. 596 ± 0. 082)、(0. 378 ± 0. 064) vs. (0.543 ±0.072)、(0.348 ± 0. 069) vs. (0. 523 ± 0. 076),皆 P <0. 01;CD40 为(0. 398 ± 0. 066) vs. (0. 546 ± 0. 079)、(0. 461 ± 0. 085) vs. (0. 618 ± 0. 076)、(0. 453 ± 0. 087) vs. (0. 587 ± 0. 074)、(0. 449 ± 0. 065) vs. (0. 565 ± 0.082),皆P<0.05 ]. 感染后,ICOSL-KO小鼠IFN-γ表达显著高于野生型小鼠(P<0.05),而IL-4表达水平则显著低于野生型小鼠(P<0. 05),其Th2分化指数亦显著低于野生型小鼠(P<0. 01). ICOSL-KO小鼠血清SEA特异性抗体IgG、IgG1、IgG2a的水平与IgG1/IgG2a的比值亦显著低于野生型小鼠(P<0. 01),且ICOSL-KO小鼠的肝虫卵肉芽肿体积显著小于野生型小鼠(P<0. 01). 结论:ICOS-ICOSL信号通路在血吸虫病免疫病理中起重要调控作用,其对Th1/Th2偏移的影响可能是通过调控CD154-CD40信号通路来实现的.     

Yu Peilan(虞佩兰)A Famous Pediatrician of Integrative Medicine

Dr.YuPeilan,female,wasborninFeb.1921,Beijing.SheisProf.ofPediatrics,andtutorofDoctorate(1987-1998).AftergraduatingfromXiangyaMedicalCollege(6years)in1946shegotherDoctor'sdegreeofmedicine.SheworkedintheDept.ofmedicine(1946-1951)andDept.ofPediatrics(1951-1998)ofXiangyaHospital,HunanMedicalUniversity.ShewasappointedtoattendaspecialtrainingcoursefortraditionalChinesemedicine(TCM)inBeijingTraditionalChineseMedicalCollegefor3years(1958-1961).In1956and1978,shewaspromotedassociateProf.andt…     

心血管常见病的高危发病因素社区性预防的实验研究

目的探讨心血管系心身疾病社区性预防的途径和方法。方法将本市两单位的 40 0名健康职工分两组 ,对其心血管系如冠心病等的心身疾病的预防知识的学习、改变不良生活方式和调整不良情绪的指导等预防干预进行前瞻性对照研究。结果经上述干预 1年后 :①两组心血管常见心身疾病的发病高危因素的临床生化指标 :血胆固醇 (TC)实验组 ( 4 .3 3± 0 .75 ) ,对照组 ( 4 .75± 0 .87)、血甘油三脂 (TG)实验组 ( 1.66± 0 .74) ,对照组 ( 1.93± 0 .85 ) ;血高密度脂蛋白 (HDL C)实验组 ( 1.13± 0 .19) ,对照组 ( 1.0 2± 0 .2 2 )、全血粘度 (mPa .s :)实验组 ( 1.5 8± 0 .14 ) ,对照组 ( 1.64± 0 .17) ,两组比较差异有显著性 (P <0 .0 5 ) ,实验组低于对照组 ;②生活方式上食盐的摄入干预前 ( 1.5 3± 0 .5 5 ) ,干预后 ( 1.78± 0 .69)、进食新鲜蔬菜干预前 ( 1.76± 0 .5 8) ,干预后 ( 1.98± 0 .71)、进食肥肉及动物内脏干预前 ( 1.69± 0 .78) ,干预后 ( 2 .0 4± 0 .84)、定时适量进食干预前 ( 2 .0 4± 0 .69) ,干预后 ( 2 .3 3± 0 .67)、体育锻炼时间干预前 ( 1.5 4± 0 .67) ,干预后 ( 1.83±0 .5 6)、锻炼项目干预前 ( 3 .17± 1.0 2 ) ,干预后 ( 3 .5 5± 1.15 )、心情平静的时间干预前 ( 1.73± 0     

大鼠慢性压迫性脊髓损伤及减压后神经营养素的表达

目的　观察神经营养素在慢性压迫性脊髓损伤后及减压后的表达变化 .方法　同龄 Wistar大鼠 6 0只 ,设 A组正常对照组、B组行后路渐进式慢性压迫、C组减压组、D组手术组 ,应用免疫组化方法观察各组脑源性神经营养因子、(BD-NF ) ,胶质源性神经营养因子 (GDNF) ,神经营养素 - 3(NT-3) ,神经营养因子 (NGF)及其受体 Trk A,Trk B,Trk C表达的变化 .结果　行慢性压迫后及减压后神经营养素及其受体表达变化明显 ,各组差异有显著性意义 .与正常组与假伤组比较 ,慢性压迫后 1d的标记指数 BDNF(32 .4± 1.6 ) vs(4.8± 1.0 ) ,GDNF(2 1.2± 3.8) vs(3.7± 1.1) ,NT- 3(2 6 .2±2 .8) vs (5 .5± 0 .6 ) ,NGF (9.2± 0 .9) vs(1.7± 0 .4 ) ,Trk A(2 3.7± 1.7) vs (0 .6± 0 .5 ) ,Trk B (34.3± 3.3) vs(3.2± 0 .5 ) ,Trk C(30 .1± 3.9) vs(1.2± 0 .4 ) (P<0 .0 5 ) .与正常组与压迫 30 d组比较 ,减压后 1d的标记指数 BDNF(30 .2± 1.6 ) vs(5 .8± 1.8) ,GDNF (2 0 .0± 3.6 ) vs(3.6± 1.1) ,NT- 3(2 5 .8± 2 .3) vs(5 .6± 0 .5 ) ,NGF(8.7± 0 .7) vs(1.7± 0 .4 ) ,Trk A(2 1.4± 4 .2 ) vs(0 .7± 0 .4 ) ,Trk B(32 .7± 3.3) vs(3.5± 0 .5 ) ,Trk C (2 8.7± 3.8) vs (1.2± 0 .6 ) (P<0 .0 5 ) .结     

Treatment of 30 Cases of Vertebrobasilar Transient Ischemic Vertigo with Buyang Huanwu Decoction (补阳还五汤)

Vertebrobasilartransientischemicvertigo(VBTIV)oftenoccurredinmiddleandoldage.FromJanuary1990toApril1995,theauthorstreated30casesofthediseasebyBuyangHuanwuDecoction(BYHWD)andobservedtheclinicaleffectandhemorrheologicindexes.Itwasreportedasfollows.METHODSClinicalMaterialsFiftythreepatientsofVBTIVdiagnosedbyclinicalandradiologicexamination,CT,transcranialDoppler(TeD)sonographicdetermination,brainstemevokedpotentialandrheoencephalogramwereobserved.Thediag.nosticstandard.e.e(1'2):(1)P…     

中药干预肺肾阴虚型癌因性疲乏

目的:观察滋肾养肺汤治疗肺肾阴虚型癌因性疲乏的临床疗效及安全性。方法:60例肺肾阴虚型癌因性疲乏患者随机平均分为观察组和对照组。对照组给予常规治疗,观察组在对照组治疗基础上给予滋肾养肺汤,两组均治疗14 d。比较两组治疗前后Piper疲乏量表评分、中医证候积分、体质量。结果:观察组有效率为86. 66%,对照组有效率为25. 00%,两组比较,差异有统计学意义(P 0. 01)。观察组治疗前后疲乏量表总评分分别为(7. 34±0. 44)分、(6. 24±0. 53)分,对照组分别为(7. 28±0. 33)分、(7. 20±0. 47);观察组行为疲乏评分分别为(6. 54±0. 50)分、(5. 80±0. 56)分,对照组分别为(6. 62±0. 39)分、(6. 51±0. 25)分;观察组情感疲乏评分分别为(8. 02±0. 58)分、(6. 76±0. 62)分,对照组分别为(7. 99±0. 61)分、(7. 76±0. 23)分;观察组感知疲乏评分分别为(7. 89±0. 53)分、(6. 55±0. 60)分,对照组分别为(7. 87±0. 49)分、(7. 70±0. 45)分;观察组认知疲乏评分分别为(7. 68±0. 60)分、(6. 57±0. 68)分,对照组分别为(7. 71±0. 54)分、(7. 49±0. 54)分;两组患者治疗前后以上各项评分比较,差异均有统计学意义(P 0. 05);观察组治疗后评分均低于对照组治疗后,差异均有统计学意义(P 0. 05)。观察组治疗前后体质量分别为(58. 13±4. 32) kg、(58. 93±4. 37) kg,差异有统计学意义(P 0. 05)。结论:滋肾养肺汤可安全、有效改善肺肾阴虚型癌因性疲乏患者的疲乏症状,可降低Piper疲乏量表评分,提高肿瘤患者生存质量。     

应用血栓弹力图比较健康成人与大鼠凝血功能的试验研究

目的应用血栓弹力图(Thromboelastography,TEG)对比健康成人与SD(Sprague Dawley)大鼠的凝血功能。方法收集20名健康成人和20只SD大鼠的全血,在2 h内完成TEG检测,并对凝血反应时间(reaction time,R)、血块形成速率(kinetics time,K)、血块形成动力学(Angle,α)、血块最大强度(maximum amplitude,MA)、30 min纤维蛋白溶解度(lysis percent at 30 min,LY30)和凝血综合指数(coagulation index,CI)参数进行统计学分析。结果健康大鼠的R时间[1. 7 min(1. 2～1. 9 min)]和K时间[0. 9 min(0. 8～1. 0 min)]比成人R时间[7. 8 min(IQR:6. 6～9. 6 min)]和K时间[2. 2 min(1. 8～3. 1 min)]显著缩短(P 0. 001);与健康成人α角[56. 4°(48. 0°～64. 2°)]比较,大鼠的α角[76. 9°(76. 3°～82. 0°)]显著升高(P 0. 0 001);与健康成人[60. 9 mm(57. 2～64. 6 mm)]相比,大鼠的MA值[75. 0 mm(70. 6～79. 4 mm)]显著升高(P 0. 001);与健康成人[0. 4%(0. 0%～0. 9%)]相比,大鼠的LY30[0. 0%(0. 0%～0. 0%]显著降低(P=0. 003);与健康成人[-2. 2(-4. 1～0. 0)]相比,大鼠的CI值[5. 8(5. 2～6. 9)]显著升高(P 0. 001)。结论与健康成人相比,大鼠的凝血功能呈显著高凝状态,主要表现在凝血因子活性、纤维蛋白原和血小板功能相对增强,纤溶相对抑制。     

青少年特发性脊柱侧凸48例患者的静态平衡能力研究

目的研究青少年特发性脊柱侧凸(AIS)患者的静态平衡能力,为其进行有效的康复治疗提供理论数据支持。方法选取2018年1月至2019年1月在天津医院康复科就诊的48例AIS患者作为AIS组,另外选取30名同期体检健康的正常青少年作为对照组,采用平板式足底压力测试系统对两组受试者双足睁眼站立、双足闭眼站立、左足睁眼站立、右足睁眼站立、左足闭眼站立、右足闭眼站立6种姿势进行测试。结果双足睁眼站立时,AIS组水平距离大于对照组[8. 07 (5. 86,13. 43) mm比5. 78 (3. 85,10. 87) mm,P <0. 05]。左足睁眼站立时,AIS组重心移动的轨迹长度、包络面积、平均速度、水平距离和垂直距离大于对照组[386(289,506) mm比299(250,354) mm、800(479,1 945) mm~2比330(172,693) mm~2、30. 35(21. 03,43. 07) mm/s比21. 94(17. 09,27. 02) mm/s、26. 93(18. 63,41. 64) mm比16. 92 (13. 62,24. 29) mm、38. 71 (26. 27,66. 48) mm比20. 84 (17. 03,39. 79) mm,P <0. 05],AIS组单位面积的轨迹长度小于对照组[0. 45(0. 29,0. 73)比0. 83 (0. 55,1. 38),P <0. 05]。右足睁眼站立时,AIS组重心移动的轨迹长度、包络面积、平均速度、水平距离和垂直距离大于对照组[385(342,511) mm比346(322,395) mm、610 (319,1 215) mm~2比350(252,688) mm~2、26. 73(20. 36,36. 97) mm/s比19. 18(15. 89,25. 93) mm/s、22. 89(17. 06,31. 55) mm比18. 94(15. 14,24. 12) mm、35. 82 (20. 12,52. 25) mm比22. 89 (16. 44,34. 13) mm,P <0. 05],AIS组单位面积的轨迹长度小于对照组[0. 65(0. 43,1. 08)比1. 01(0. 62,1. 50),P <0. 05]。结论青少年特发性脊柱侧凸患者的静态平衡能力低于正常健康青少年,姿势调节能力低于正常青少年。     

逐瘀通脉胶囊联合法舒地尔对2型糖尿病下肢动脉硬化闭塞症患者疗效及应激状态的影响

目的:探讨逐瘀通脉胶囊联合法舒地尔对2型糖尿病(T2DM)下肢动脉硬化闭塞症(ASO)患者疗效及应激状态的影响。方法:将100例T2DM合并ASO患者分为研究组(50例)和对照组(50例),对照组采用法舒地尔治疗,研究组采用逐瘀通脉胶囊联合法舒地尔治疗,对比两组治疗前后动脉硬化指标[踝肱指数(ABI)、足背动脉血流量]、血流变指标[血浆黏度、全血黏度(低切)、全血黏度(高切)]、凝血功能指标[血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血浆纤维蛋白原(FIB)]、氧化应激指标[丙二醛(MDA)、超氧化物歧化酶(SOD)、硫氧还蛋白(TRX)、髓过氧化物酶(MPO)活性]、跛行距离,观察两组疗效和安全性。结果:研究组治疗后ABI、足背动脉血流量、跛行距离高于对照组[(0. 99±0. 16)vs(0. 81±0. 12),(0. 79±0. 27)vs(0. 57±0. 09)m/(s·cm~2),(569. 93±135. 34)vs(415. 72±112. 28)m/10 min],差异具有统计学意义(P<0. 05)。研究组治疗后血浆黏度、全血黏度(低切)、全血黏度(高切)、FIB低于对照组[(2. 00±0. 26)vs(2. 31±0. 32)mPa/s,(13. 79±1. 27)vs(20. 57±2. 05)mPa/s,(8. 93±0. 34)vs(11. 72±1. 28)mPa/s,(2. 73±0. 35)vs(3. 49±0. 43)g/L,P<0. 05],PT、APTT高于对照组[(12. 85±2. 63)vs(11. 93±1. 74)s,(42. 95±5. 36)vs(36. 95±4. 38)s,P<0. 05]。研究组治疗后MDA、TRX、MPO低于对照组[(2. 10±0. 96)vs(4. 31±1. 32)mmol/L,(48. 93±10. 34)vs(78. 72±12. 28)ng/mL,(72. 85±15. 63)vs(96. 03±12. 14)U/L,P<0. 05],SOD高于对照组[(163. 79±21. 27)vs(110. 57±12. 05)nU/mL,P<0. 05]。研究组总有效率高于对照组(94. 00%vs 80. 00%,χ~2=13. 025,P<0. 05),两组不良反应率(6. 00%vs 10. 00%,χ~2=1. 035,P>0. 05)对比无差异。结论:逐瘀通脉胶囊联合法舒地尔可显著改善T2DM合并ASO患者临床症状,改善下肢局部血液循环,抑制应激反应,疗效确切,安全性高。     

锥形束CT对切牙管及其相对位置关系的测量分析

目的:应用锥形束CT( cone-beam computed tomography,CBCT)测量切牙管的相关参数,及切牙管与嵴顶、中切牙的相对位置关系,为临床前牙种植提供指导,希望能在前牙种植中规避损伤的风险,制定完善的种植计划.方法:选取129例种植患者的CBCT资料进行测量,分别截取切牙管矢状面、经切牙管长轴的冠状面、横断面的图片测量以下指标:低位矢径(Sda)、中位矢径(Sdb)、高位矢径(Sdc),并求得平均矢径(Sd),低位横径(Hda)、中位横径( Hdb)、高位横径( Hdc) ,并求得平均横径( Hd) ,长度( H) ,切牙管最低点唇侧骨厚度( Bt) ,与嵴顶距离( At) ,与中切牙的距离(Id). 根据患者性别、年龄进行分组,采用SPSS 17. 0统计学软件进行统计学分析,以P<0. 05为差异有统计学意义. 结果:总体Sd为(3. 41 ± 0. 87) mm,Hd为(5. 16 ± 0. 93) mm,两者间差异有统计学意义. H为(14. 29 ± 3. 27) mm,Bt为(7. 49 ± 1. 05) mm,At为(8. 25 ± 1. 71) mm,Id为(2. 71 ± 0. 89) mm. 男、女平均Sd分别为(3. 64 ± 0. 90) mm、(3. 28 ± 0. 82) mm,P=0. 017;Hd分别为(4. 98 ± 0. 89) mm、(5. 27 ± 0. 94) mm,P=0. 081;平均H分别为(15. 47 ± 2. 75) mm、(13. 59 ± 3. 32) mm,P=0. 001;平均Bt分别为(7. 90 ± 0. 96) mm、(7. 25 ± 1. 03) mm,P=0. 001;平均At分别为(7. 61 ± 1. 45) mm、(8. 64 ± 1. 74) mm,P=0. 001;平均Id分别为(2. 71 ± 0. 87) mm、(2. 72 ± 0. 91) mm,P=0. 983. 按照年龄分组,Sd各组间P=0. 325,Hd各组间P=0. 636,H各组间P=0. 292,Bt各组间P=0. 116,At各组间P=0. 010,Id各组间P<0. 001. 结论:性别对切牙管的长度、唇侧骨厚度以及与嵴顶的位置关系是有影响的,年龄对于切牙管本身的参数没有影响,但是对于切牙管与嵴顶和中切牙的相对位置有一定影响;CBCT测量在种植设计中是非常有价值的.     

Anticancer Effect of Curcumin on B Cell non- Hodgkin''s Lymphoma

To explore the anticancer effect of curcumin on human B cell non-Hodgkin's lymphoma and compare its effects on human B cell non-Hodgkin's lymphoma cells and normal peripheral blood mononuclear cells (NPBMNCs). MTT assay was used to study the effect of curcumin on the growth of Raji cells and NPBMNCs. The effect of curcumin on the apoptosis of Raji cells and NPBMNC were studied by flow cytometry and TDT-mediated dUTP nick and labeling (TUNEL). The effect of curcumin on the cell cycle of Raji cells were examined by propidium iodide staining flow cytometry. The results showed that curcumin strongly inhibited ±1.82 μmol/L and curcumin induced Raji cell apoptosis in a time- and dose-dependent manner. Raji cells treated with curcumin showed curcumin did not demonstrate apparent proliferation inhibition and apoptosis induction in NPBMNCs. It was concluded that curcumin is able to inhibit the proliferation of Raji cells by regulating the cell cycle and inducing the cell apoptosis. Morever, curcumin has low toxicity on NPBMNCs but can selectively induce apoptosis in Raji cells.     

Anticancer Activities of Trichostatin A on Maligant Lymphoid Cells

The anticancer activity of trichostain A (TSA) on human B cell non-Hodgkin's lymphoma and its mechanism were explored. The effect of TSA on the growth of Raji cells and normal peripheral blood mononuclear cells (NPBMNC) was studied by MTT assay. The effect of TSA on the apoptosis of Raji cells and NPBMNC was studied by flow cytometry and TDT-mediated dUTP nick end labeling (TUNEL). The effect of TSA on the cell cycle of Raji cells was studied by propidium iodide method. The results showed that TSA potently inhibited proliferation of Raji cells at microgram concentrations and induced apoptosis of Raji cells in a time- and concentration-dependent manner. Treatment with TSA induced accumulation of cells in G0/G1 or G2/M and a concomitant decrease of cell population in S phase. However, NPBMNC was less sensitive to the cytotoxic effect of TSA than Raji cells. It was concluded that TSA may inhibit the proliferation of Raji cells by regulating the cell cycle and inducing the cell apoptosis. Moreover, TSA demonstrates low toxicity in NPBMNC but selectively induces apoptosis of Raji cells.     

HDAC1 expression and effect of curcumin on proliferation of Raji cells

Summary Histone deacetylase (HDAC₁) has a high expression in many cancer cells and curcumin can inhibit the growth of cancer cells. This paper was designed to investigate the expression of HDAC₁ of Raji cells and the effect of curcumin on their proliferation and apoptosis. Raji cells were treated with 3. 125–50 μmol/L curcumin for 8–48 h and the growth inhibition rates of Raji cells were measured by MTT. The expression of HDAC₁ on Raji cells were examined by mRNA, Western blot at 24 h various concertrations (1.6–50 μmol/L). Curcumin could selectively inhibit the proliferation of Raji cells in a dose and time dependent manner, with the inhibition rate being 52.47%–82.18% (P<0.01). The up-regulation of HDAC₁ expression was observed within 24 h after the treatment with curcumin as shown by RT-PCR and Western blot. With the increase of concentration, the expression was down-regulated in a dose dependent manner. It is concluded that the expression of HDAC₁ plays an important role in the proliferation and apoptosis of Raji cells and curcumin can inhibit the growth of Raji cells at various concentrations and promote the apoptosis of Raji cells. WU Qing, female, born in 1970, M. D., Ph. D. This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30271672).     

Curcumin down-regulates PCNA, cyclin D1 and Bcl-XL expression in human keratinocyte cell lines

Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte lines（HaCaT cells） were treated with different doses of curcumin.The effects of curcumin on cell viability were measured by MTT assay,and the cell cycle distribution and apoptosis determined by flow cytometry.The mRNA expression changes of proliferating cell nuclear antigen（PCNA）,cyclin D1 and Bcl-xL were from real-time PCR analysis and the protein levels were detected by Western blotting.Results：Data obtained in the study showed that curcumin could cause significantly inhibitory effect on proliferation in HaCaT cells in a time- and dose-dependent manner.Cell arrest at G1/S phase and significant apoptosis were observed after being treated with curcumin for 24 h.In association with these,the expression of PCNA,cyclin D1 and Bcl-xL were decreased both at mRNA and protein levels for the same treatment.Conclusion：Curcumin can inhibit proliferation,induce cell arrest at G1/S phase and cause apoptosis in HaCaT cells.The decreased expression of PCNA,cyclin D1 and Bcl-xL induced by curcumin contributes to the above effects in vitro.     

汉防己甲素对人神经母细胞瘤SH-SY5Y增殖和凋亡的影响

目的 探讨汉防己甲素对神经母细胞瘤SH-SY5Y细胞株的增殖抑制和诱导凋亡的作用。方法 通过CCK-8实验检测汉防己甲素对于细胞生长的抑制率,流式细胞术检测Tet作用于细胞后,对细胞活性氧、线粒体膜电位、凋亡和周期的影响。结果 汉防己甲素能显著抑制SH-SY5Y细胞生长,基本呈时间－剂量依赖性。流式细胞术检测显示不同浓度汉防己甲素处理后,活性氧明显升高,线粒体膜电位明显下降,细胞凋亡比例明显升高,G₀/G₁期细胞占比明显增高。结论 汉防己甲素能够抑制SH-SY5Y细胞增殖并且诱导其发生凋亡。     

虫草素对人骨肉瘤MG-63细胞凋亡的影响及其机制研究

目的 研究虫草素对人骨肉瘤MG-63细胞凋亡的影响及其机制。方法 采用CCK-8实验检测不同浓度虫草素作用于MG-63骨肉瘤细胞24h和48h后对癌细胞增殖的抑制作用;采用流式细胞术分析虫草素对MG-63细胞周期分布及凋亡的影响;采用Western blot法检测虫草素对MG-63细胞凋亡相关蛋白Bax、Bcl-2、cleaved caspase-9、cleaved caspase-3及NICD1、Hes1蛋白表达的影响。结果 虫草素对人骨肉瘤MG-63细胞有明显的增殖抑制作用,导致MG-63细胞周期阻滞于G₀/G₁期并诱导细胞凋亡;虫草素可上调Bax、cleaved caspase-9和cleaved caspase-3蛋白的表达并下调Bcl-2,NICD1和Hes1蛋白的表达。结论 虫草素通过抑制骨肉瘤细胞增殖,诱导细胞周期阻滞于G₀/G₁期并通过线粒体凋亡途径诱导MG-63细胞凋亡从而发挥抗骨肉瘤作用,可能骨肉瘤细胞中Notch信号通路活性下调有关。     

黄芪注射液对乳腺癌MCF-7细胞增殖周期以及细胞凋亡、迁移影响的实验研究

目的 探讨黄芪注射液对乳腺癌MCF-7细胞增殖、周期、凋亡及迁移的影响。方法 通过MTT法观察药物处理后对乳腺癌MCF-7细胞的生长增殖情况的影响;通过流式细胞术检测黄芪注射液对乳腺癌MCF-7细胞增殖周期、凋亡的影响;利用划痕实验检测黄芪注射液对乳腺癌MCF-7细胞的迁移的影响。结果 MTT结果发现,不同浓度的黄芪注射液（100、200、400、600、800mg/ml）对乳腺癌MCF-7细胞的增殖具有不同程度的抑制作用;流式细胞术结果显示,与空白对照组相比,200和400mg/ml黄芪注射液均能导致G₁期增加,且两个浓度的凋亡率都大于空白对照组;划痕实验结果显示200和400mg/ml黄芪注射液组较空白对照组划痕愈合率低。结论 不同浓度的黄芪注射液在体外对乳腺癌MCF-7细胞增殖具有一定的抑制作用;同时可将细胞增殖周期阻滞在G₁期,促进MCF-7细胞凋亡;一定浓度的黄芪注射液对MCF-7细胞的迁移具有抑制作用,为乳腺癌的临床应用提供实验基础。     

Effects of concurrent use of rh-IFN-γ and curcumin on the antiproliferative capacity of HL-60 cells

Summary To understand the effect of rh-IFN-γ on the ability of curcumin to kill HL-60 cellsin vitro, the myeloid leukemic cell line HL-60 was studied by using cell culture. BrdU incorporation rate was examined by SABC, DNA content was determined by flow cytometry and apoptotic cell percentage was determined by TUNEL method. The results showed that curcumin inhibited proliferation of leukemic cells in a dose-dependent manner. When HL-60 cells were treated with 25 μmol curcumin for 24 h, the proliferative inhibitory rate was 43. 75±2. 00 %. This effect could be enhanced obviously by IFN-γ, the combined proliferative inhibitory rate increased to over 80 %. The 5-BrdU incorportion rate and the distribution of DNA content indicated that curcumin could arrest cells in the G₁,/G₀ and G₂/M phase of cell cycle. At the same time, the sub-G₁ peak (apoptotic peak) appeared. After IFN-γ combined with curcumin DNA synthesis rate decreased further. It showed a significant difference when compared with single drug group (P < 0. 05). Meanwhile, sub-Gi peak also increased. The percentage of TUNEL positive cells was aslo increased. It is concluded that IFN-γ can enhance the antiproliferative ability of curcumin against HL-60 cells.     

The influence of curcumin on the cell cycle of Hl-60 cells- and contrast study

Over the past years, the new research findingshave shown that cancer development depends onwhether normal cell cycle is permanently disturbed.Abnormal cell cycle may contribute to uncontrolledcell growth, ace urn "lating. mutat ion and fin al c arc ino-genesis instead of progammed cell death (PCD)[1]. Itis now widely accepted that curcumin possesses definite anticancer effects by inhibition of the cancer cellproliferation. In order 'to understand further themechanism of curcumin against acute…     

Growth-inhibiting and apoptosis-inducing effects of Tanshinone II A on human gastric carcinoma cells

Summary To explore the effects of Tanshinone II A on the proliferation, apoptosis and gene expression of p53 and bcl-2 in human gastric carcinoma MKN-45 cells. Cell count and MTT assay were used to study the proliferation-inhibiting effect of Tanshinone II A on MKN-45 cells. The effect of Tanshinone II A on the cell cycle and apoptosis of MKN-45 cells were examined by propidium iodide (PI) staining and flow cytometry. Semi-quantitative RT-PCR was used to further verify the expression of p53 and bcl-2 gene after exposure to Tanshinone A in MKN-45 cells. The results showed that Tanshinone A significantly inhibited the growth and proliferation of MKN-45 cells in a dose-and time-dependent manner (P<0.05). Tanshinone A arrested MKN-45 cells in G₂/M phase which led to an obvious accumulation of G₂/M phase cells while decreased number of G₀/G₁ phase cells. This resulted in apoptosis of MKN-45 cells and the apoptosis rate was as high as 43.91% after treatment with 2.0 μg/mL Tanshinone II A for 96 h. It was also found that Tanshinone II A up-regulated expression of p53 gene and down-regulated expression of bcl-2 gene. The cytostatic and antiproliferative effect of Tanshinone II A makes it a promising anticancer agent for the treatment of gastric carcinoma.