乌蕨对砷和铵的解毒作用

观察了乌蕨的4种提取物对小鼠砷和氯化铵急性中毒的解毒作用。结果表明:乌蕨提取物B及C能明显降低小鼠的死亡率,提取物C尚可使小鼠对砷耐受量提高,使其LD₅₀从31.1±4.3mg/kg提高到38.2±5.9mg/kg。4种提取物对小鼠NH₄Cl中毒虽可降低其死亡率,但与对照组相比,差异无显著意义(P>0.05)。     

The protective effect of Allium sativum L. clove aqueous and methanolic extracts against hypoxia-induced lethality in mice

The antihypoxic activity of Allium sativum clove (garlic) aqueous and methanolic extracts was studied in mice.The extracts of garlic showed that the antihypoxic effect was dose-dependent. The minimum effective doses of aqueous and methanolic extracts were 0.2 g/kg and 5.12 g/kg, respectively. Phenytoin, 50 mg/kg, and R-phenylisopropyladenosine (R-PIA), 1.6 mg/kg (R-PIA) as positive controls increased survival time up to 52.5 +/- 2.9 min and 120.5 +/- 6 min, respectively, compared to normal saline (34.73 +/- 0.71 min). The high doses of aqueous (16.9 g/kg) and methanolic (12.8 g/kg) extracts increased survival time up to 73.17 +/- 4.9 and 68.41 +/- 3.7, respectively.These results indicated that the extracts of A. sativum cloves have a protective effect against hypoxia-induced lethality in mice.     

Hypoglycemic effect and chlorogenic acid content in two Cecropia species

The hypoglycemic effect of methanol leaf extracts from Cecropia obtusifolia and C. peltata was evaluated in healthy mice. A significant decrease (p < 0.05) in plasma glucose levels was recorded 2 and 4 h after a single oral administration of methanol extracts (1 g/kg). This effect was correlated with the chlorogenic acid contents in both species; C. peltata, containing 19.84 +/- 1.64 mg of chlorogenic acid/g of dried leaves produced the highest decrease (D(alpha 2,60) = 20.18, p < 0.05) of plasma glucose levels (52.8%). The extracts of C. obtusifolia from Tabasco and Veracruz, showed similar hypoglycemic effects (33.3% and 35.7%, respectively) and chlorogenic acid contents (Tukey(0.05) = 1.8859) (13.3 +/- 3.2 mg/g and 13.1 +/- 1.6 mg/g, respectively). The hypoglycemic effect produced by different doses (0.1, 0.25, 0.50, 0.75 and 1 g/kg body wt, p.o.) of C. peltata showed a lineal relationship with chlorogenic acid content, reaching an ED(50) = 0.540 g/kg body wt for extract, and an ED(50) = 10.8 mg/kg body wt for chlorogenic acid. These results suggest that C. peltata is a better hypoglycemic agent than C. obtusifolia, and it could be considered for developing a phytomedicinal product to carry out clinical trials.     

In vivo antimalarial activities of Quassia amara and Quassia undulata plant extracts in mice

Extracts obtained from two Nigerian Simaroubaceae plants, Quassia amara L. and Quassia undulata (Giull and Perr) D. Dietr were screened for antimalarial properties using a total of six extracts. The plant extracts showed significant antimalarial activities in the 4 day suppressive in vivo antimalarial assay in mice inoculated with red blood cells parasitized with Plasmodium berghei berghei. Plant extracts were studied at 100 mg and 200 mg per kg body weight mouse per day, respectively. At a concentration of 100 mg/kg of mouse, Q. amara leaf hexane extract had the highest suppressive activity with a parasite density of 0.16 +/- 0.001%. Q. amara leaf methanol extract had an outstanding activity; of 0.05 +/- 0.03% at 200 mg/kg. Chloroquine (10 mg/kg, positive control) had a suppressive activity of 0.34 +/- 0.02 in the same assay on day 4.     

Cardiovascular effects of the South American medicinal plant Cecropia pachystachya (ambay) on rats

Cecropia pachystachya is used in South America for relieving cough and asthma. In Argentina it is known as "ambay" and grows in the neotropical forests (Ntr C.p.) and in temperate hilly regions (Tp C.p.). To evaluate their cardiovascular profile, the effect of extracts obtained from plants growing in the neotropical region as well as in temperate areas were compared by i.v. administration in normotensive rats. The following parameters were measured: blood pressure (BP) and heart rate (HR). The hypotensive effect was stronger for Ntr C.p., which aqueous extract decreased BP at doses between 90 and 300 mg lyophilised/kg until 46.2 +/- 12% of basal. The extract of Tp C.p. reduced BP to 86.1 +/- 11% of basal (p < 0.05 respect to Ntr C.p.) at 180 mg/kg, but increased HR at 90 and 180 mg/kg (until 133.6 +/- 10.8% of basal, p < 0.05) and produced death by respiratory paralysis at 320 mg/kg (about 3g dry leaves/kg). The hypotensive effects, but not the chronotropic ones, were attenuated by pretreatment with reserpine (5 mg/kg). The plant extracts had not diuretic activity by oral administration in conscious rats, nor produced vasodilation of perfused hindquarters arterial bed precontracted with high-[K] or 100 microM phenylephrine. The results suggest that neotropical ambay is more hypotensive than the one from the temperate hilly region. When it reaches plasma, it could produce hypotension (by central blockade of sympathic innervation of vessels) and tachycardia (by central cholinergic inhibition of heart), although it happens at doses higher than the oral ethnotherapeutic (about 340 mg dried leaves/kg).     

番石榴叶提取物对db/db小鼠骨骼肌AMPK-PGC-1α-FNDC5信号通路的影响

目的:探讨番石榴叶提取物对糖尿病 db/db 小鼠骨骼肌 AMPK-PGC-1α-FNDC5信号通路的影响。方法:实验共纳入24只6周龄雄性db/db小鼠及6只同龄雄性C57BL/6J小鼠,适应性喂养两周后,6只C57BL/6J雄性小鼠作为正常组,db/db小鼠检测空腹血糖≥7.0 mmol/l者视为造模成功,并随机分为模型组和番石榴叶提取物高、中、低剂量组,每组各6只。番石榴叶提取物按高剂量14 mg/kg、中剂量7 mg/kg、低剂量3.5 mg/kg每日进行灌胃,正常组小鼠灌服等量蒸馏水,干预4周。每周监测小鼠进食量、体重和空腹血糖。 实验结束后,将小鼠空腹脱臼处死,并从眼眶取血,测定血浆胰岛素水平。Western-blot检测AMPK-α、p-AMPK-α、PGC-1α、FNDC5蛋白在骨骼肌中的表达,以及PGC-1α、UCP-1在脂肪组织中的表达。结果:番石榴叶提取物高剂量组小鼠相比模型组小鼠体重、血糖均显著降低,中剂量组小鼠在第2周血糖也出现明显下降,高、中剂量组小鼠的胰岛素水平均降低。检测蛋白表达结果显示,番石榴叶提取物干预的小鼠骨骼肌P-AMPK-α、PGC-1α、FNDC5蛋白和脂肪组织PGC-1α、UCP-1蛋白的表达均明显高于模型组。结论:番石榴叶提取物可通过增强骨骼肌的AMPK-PGC-1α-FNDC5信号通路传导,诱导白色脂肪组织棕色化,以实现降低血糖、减轻体重。     

Flavonoid of Drynaria fortunei protects against acute renal failure

The flavonoid fraction (FF) from Drynaria fortunei was investigated to determine its biological activity expression in three acute renal failure animal models.Guinea pigs received 100 mg/kg of gentamicin (GM group), 100 mg/kg of GM plus 10 mg/kg of FF (GMFF group), 10 mg/kg of FF (FF group) or saline (saline group) intramuscularly for 14 days. The blood urea nitrogen (BUN) and creatinine levels were found to be significantly higher in the GM group (22.70+/-3.84 mg/dL, 0.68+/-0.05 mg/dL) than in the GMFF group (17.10+/-1.04 mg/dL, 0.58+/-0.09 mg/dL), the FF group (17.40+/-1.01 mg/dL, 0.49+/-0.20 mg/dL) and the saline group (17.50+/-1.22 mg/dL, 0.50+/-0.02 mg/dL).Mice were treated once with 6 mg/kg of mercuric chloride, followed by 10 mg/kg of FF or saline. On days 3, 4 and 5, BUN and creatinine levels were found to be significantly higher in the HgCl2-saline group (74.00+/-39.20 mg/dL, 59.30+/-31.20 mg/dL, 74.00+/-37.30 mg/dL and 0.53+/-0.17 mg/dL, 0.48+/-0.15 mg/dL 0.33+/-0.15 mg/dL) than in the HgCl2-FF group (19.50+/-4.90 mg/dL, 43.00+/-26.30 mg/dL, 38.50+/-13.80 mg/dL and 0.23+/-0.05 mg/dL, 0.30+/-0.12 mg/dL, 0.15+/-0.06 mg/dL).After surgery for 5/6-nephrectomy, ten mice received FF at a dose of 10 mg/kg/day and eight received saline for 42 days. The saline group survived for 12-62 days and the FF group survived for 20-320 days. The FF group had a significantly longer survival time than the saline group (p<0.05). Regeneration of kidney tubular cells and significantly enlarged convoluted tubules were noted in the pathology study of the FF group.In conclusion, the present study suggests that FF prevents nephrotoxicity, improves kidney function and promotes kidney primary epithelial tubular cell regeneration.     

Antiulcerogenic effects of Tylophora conspicua in male rats

Studies were undertaken on the effect of a crude (TC) and an alkaloid fraction (TA) of the leaf extracts of Tylophora conspicua on indomethacin-induced gastric ulceration and gastric acid secretion in male albino rats. Both the TC and TA produced a dose-dependent inhibition of gastric ulceration. At a dose level of 40 mg/kg TC and TA were more effective (TA being more potent) than propranolol in inhibiting gastric ulceration. The highest dose of the extracts used (80 mg/kg) completely inhibited gastric ulceration. Intravenous administration of the TC and TA significantly decreased acid output at low dose (20 mg/kg), medium dose (40 mg/kg) and high dose (80 mg/kg) from the peak basal of 0.54 +/- 0.02 mEq/L/min to 0. 49 +/- 0.02 mEq/L/min, 0.35 +/- 0.01 mEq/L/min and 0.21 +/- 0.02 Meq/L/min respectively. 80 mg/kg of TC and TA significantly reduced the histamine (1 mg/kg) induced gastric acid secretion. The results suggest that the antiulcer activity of Tylophora conspicua might be produced by gastric acid inhibition.     

Effects of Salvadora persica extracts on mice exploratory locomotion activities

The effects of Salvadora persica extracts on mice exploratory locomotor activities and stereotype movements have been determined. Mice injected with Salvadora persica extracts showed significantly low exploratory locomotor activity. The total distance travelled by naive mice injected with saline, during the first 5 min of introduction into an activity cage (Session 1) was 1007 +/- 105 cm/5 min, in contrast to 800 +/- 75, 540 +/- 100 and 330 +/- 55 cm/5 min for naive mice injected i.p. with 5.7, 14.3 and 28.6 ml/kg Salvadora persica extract. The exploratory locomotion of Salvadora persica treated mice declined faster than that of the controls. Mice injected with Salvadora persica extract also showed a significantly lower number of stereotype movements (P less than 0.05). The stereotype movements of the control was 175 +/- 5 movements/5 min and 90 +/- 10, 118 +/- 15 and 35 +/- 11 for mice injected with 5.7, 14.3 and 28.6 ml/kg, Salvadora persica extract, respectively.     

Hepatoprotective properties of Crepis rueppellii and Anisotes trisulcus: two traditional medicinal plants of Yemen

Pharmacological investigations were carried out to evaluate the hepatoprotective effects of Crepis rueppellii and Anisotes trisulcus. Ethanolic extracts of these plants were investigated for their ability to reduce mortality of mice after ethanol intoxication and to lower the activities of plasma glutamic-pyruvic transaminase (GPT) after carbon tetrachloride-induced hepatitis in rats. Crepis and Anisotes extracts and a 50:50 mixture of both at 200 mg/kg presented significant hepatoprotective effects in both experimental situations. The traditional therapeutic indications of these plants have been largely confirmed.     

Anticonvulsant effect of Hypericum perforatum: role of nitric oxide

Hypericum perforatum L. is used in traditional medicine for its anticonvulsant property. We studied the anticonvulsant activity of the aqueous and ethanolic extracts of Hypericum perforatum aerial parts in mice in order to evaluate the traditional use of this plant. The pentylenetetrazole (PTZ) and the maximal electroshock seizure (MES) tests were used for assessing the anticonvulsive effects of this plant. In the PTZ test, the extracts (0.1-1g/kg, i.p.) delayed the onset of tonic convulsions and protected mice against mortality. In the MES test, both extracts did not showed an antiseizure activity. L-NAME (1-10 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, reduced the anticonvulsant activity of the extracts. The results of this study indicate that the extracts of Hypericum perforatum aerial parts could contribute to the control of petit mal seizure and this effect may be partially mediated by nitric oxide pathway.     

大黄对正常和烧伤小鼠肠道免疫分泌的影响

目的观察小鼠烧伤前后肠道免疫分泌的变化和大黄汤治疗对正常小鼠和烧伤小鼠肠道免疫分泌的促进作用;探讨大黄保护肠粘膜屏障的作用机理.方法实验用BALB/c小鼠.分为4组1组为对照组(n＝8);2组为正常小鼠饲喂10%大黄液12ml/kg,每6h1次(n＝11);3组为30%的全厚背部皮肤烧伤,不治疗(n＝7);4组为全厚皮肤烧伤,同3组,大黄治疗同2组(n＝9).24h后处死动物,灌洗肠道,收集肠液透析离心后作IgA检测和总蛋白,补体C3,高密度脂蛋白(HDL)测定.结果1、2、3和4组小鼠肠液中的IgA(mg/L)分别为93.5±13.7、150.8±44.6、43.4±21.5及59.8±19.3,说明在烧伤后肠液中IgA明显减少(P＜0.05).而应用大黄汤治疗后,无论正常小鼠和烧伤小鼠的肠道IgA均增加(P＜0.05),其他免疫相关物质如总蛋白、补体C3和HDL大黄治疗后也有显著增加.结论烧伤后小鼠肠液中的IgA明显减少.而大黄无论对正常小鼠和烧伤小鼠均具有明显的促进肠道免疫分泌的效果.这一作用是大黄的粘膜屏障保护作用的重要机制之一.     

Anti-inflammatory, analgesic and anti-lymphocytic activities of the aqueous extract of Crinum giganteum

The anti-inflammatory, anti-lymphocytic and analgesic properties of Crinum giganteum, a popular herb used for the management of asthma and other respiratory disorders was investigated in rats and mice. The extract dose-dependently produced significant (P<0.05) inhibition of formalin induced pain in rats. It also demonstrated significant (P<0.01) inhibition of abdominal constriction induced with 0.75% v/v acetic acid in mice. On the cotton pellet induced granulomatous tissue formation in rats, the extract significantly (P<0.05) decreased the weight. However, no significant inhibition was observed in the egg albumin-induced inflammation in rats. Oral administration of this extract in rats for 14 days significantly affected (P<0.05) the total leukocyte count and the overall percentage lymphocytes. The intraperitoneal and per oral LD(50) were 627+/-5.8mg/kg and 1486+/-18.9 mg/kg in mice and 520+/-10.2mg/kg and 1023+/-4.3 mg/kg in rats, respectively. Preliminary phytochemical analysis of the extract indicates the presence of tannins. These results therefore indicate that C. giganteum bulb contains biologically active principles, which have potentials for the treatment of inflammatory processes.     

Xanthine oxidase inhibitory activity of some Indian medical plants

Xanthine oxidase inhibitory activity was assayed from six species belonging to different families traditionally used for the treatment of gout and related symptoms by indigenous people of India. The aqueous, methanol-water mixture and methanolic extract of these plants were used for the experiment. Of the 18 extracts assayed, 14 extracts demonstrated xanthine oxidase inhibitory activity at 100 microg/ml, among which 10 extracts showed an inhibition greater than 50% and IC(50) values below 100 microg/ml. The methanolic extracts of Coccinia grandis, Datura metel, Strychnos nux-vomica and Vitex negundo showed more than 50% inhibition, hence, they were screened for their in vivo hypouricaemic activity against potassium oxonate-induced hyperuricaemia in mice. Methanolic extracts of Coccinia grandis and Vitex negundo showed a significant decrease in the serum urate level (3.90+/-0.07 mg/dl, P<0.001) and (6.26+/-0.06 mg/dl, P<0.01), respectively, when compared to hyperuricaemic control (11.42+/-0.14 mg/dl). This effect is almost similar to the serum urate level of allopurinol (3.89+/-0.07 mg/dl).     

Flavonoids from Tilia americana with anxiolytic activity in plus-maze test

The aerial parts of Tilia americana var. mexicana (Schltdl) Hardin (Tiliaceae) have been widely used in Mexican traditional medicine to relieve sleeplessness, headache, and nervous excitement. The anxiolytic effect of four extracts and several flavonoid fractions from the bracts of Tilia americana subsp.mexicana, var. mexicana (Schltdl) Hardin or Tilia mexicana (Tiliaceae) was studied. Administration of 100mg/kg of n-hexane, ethyl acetate, and aqueous extracts to elevated plus-maze (EPM)-exposed mice displayed no anxiolytic effect; however, identical doses of methanol extract was able to increase the time percentage that mice spent in the EPM's open arms, as well as the percentage of crossings in the EPM's arms. The dose-response curve produced by methanol extract showed anxiolytic activity since 25mg/kg; animals showed no motor activity alteration in the open field test (OFT). Methanol extract was subjected to a bioassay-guided fractionation to obtain four ascendant polarity fractions (F1-F4) which were administrated at 100mg/kg. Data results indicate that F1 displayed the main anxiolytic effect. The purification of F1 produced a rich flavonoid anxiolytic mixture (F1C). This fraction was purified by RP-18 open chromatographic column to obtain four polar descent fractions: F1C(1), F1C(2), F1C(3), and F1C(4), respectively. Tiliroside was the major ingredient from the active fraction. High performance liquid chromatography analysis indicated that F1C was constituted principally of tiliroside, quercetin, quercitrin, kaempherol, and their glycosides. These results supported the use of Tilia americana in Mexican traditional medicine as well as the anxiolytic effect of a rich flavonoid fraction without affect motor activity.     

暗紫贝母及浙贝母醇水提取物镇咳、祛痰及平喘作用比较研究

目的:比较暗紫贝母粉末、暗紫贝母醇提取物、暗紫贝母水提取物、暗紫贝母醇提及水提混合物、浙贝母醇提及水提混合物的镇咳、祛痰及平喘作用及作用的差异程度。方法:小鼠氨水引咳及豚鼠枸橼酸引咳法比较其镇咳作用;小鼠酚红排痰及大鼠毛细管排痰法比较其祛痰作用;整体动物引喘实验法比较其平喘作用。每个实验均分为7组,分别为阴性对照组,阳性对照组,暗紫贝母粉末组,暗紫贝母醇提物组,暗紫贝母水提取物组,暗紫贝母醇提及水提混合物,浙贝母醇提及水提混合物组,阴性组采用蒸馏水或生理盐水ig 5 d,镇咳实验中5个贝母组的剂量分别为:5.0 g.kg-1(小鼠)、1.5 g.kg-1(豚鼠),阳性对照药为磷酸可待因,剂量分别为:25 mg.kg-1(小鼠)、20 mg.kg-1(豚鼠),祛痰实验中5个贝母组的剂量分别为:1 g.kg-1(大鼠),2 g.kg-1(小鼠),阳性对照药为氯化铵,剂量为1 g.kg-1,平喘实验中5个贝母组的剂量为1.5 g.kg-1,阳性对照药为硫酸沙丁胺醇,剂量为4.5 mg.kg-1,均为ig 5 d。结果:暗紫贝母粉末镇咳、祛痰及平喘作用明显,与阴性组比较差异均有统计学意义,相同剂量下,浙贝母醇提水提混合物组与阴性组比较差异均无统计学意义,未表现出镇咳、祛痰及平喘作用,氨水引咳模型中暗紫贝母醇提物组R为143.21%,枸橼酸引咳模型中5 min内咳嗽次数与阴性组比较,P<0.05;暗紫贝母水提物祛痰作用与阴性组比较,P<0.05;暗紫贝母醇提及水提混合物祛痰作用与阴性组比较,P<0.05,其余作用与浙贝母醇提及水提混合物相当,与阴性组比较,差异无统计学意义。结论:暗紫贝母全粉给药能充分发挥其镇咳、祛痰及平喘作用;暗紫贝母镇咳有效成分可能主要集中在醇提物中,暗紫贝母祛痰有效成分可能主要集中在其水提物中;50%乙醇提取,药渣水煎可能会造成暗紫贝母部分有效成分损失;现有剂量条件下,浙贝母不能发挥其镇咳、祛痰及平喘作用。     

Evaluation of medicinal plants from Mali for their in vitro and in vivo trypanocidal activity

Water, methanol and dichloromethane extracts prepared from various parts of 40 medicinal plant species from Mali were investigated for their trypanocidal activity against Trypanosoma brucei brucei. Of a total of 165 extracts tested in vitro in the Low Inoculation Long Incubation Test (LILIT), 24 extracts showed a high trypanocidal activity. Using the Long-Term Viability Assay (LtVA) for corroboration of the results of the 24 extracts, it was found that 15 samples had minimum inhibitory concentration (MIC) values > 10 microg/ml, eight MIC values of 100 microg/ml and one MIC values of 50-100 microg/ml. So far, four extracts with MIC values < or = 100 microg/ml were tested for antitrypanosomal activity in mice, experimentally infected with Trypanosoma brucei brucei. Only, the aqueous extracts of the leaves of Terminalia avicennioides Guill. and Perr. (Combretaceae) and the stem bark of Ceiba pentandra (L.) Gaertn. (Bombacaceae) were able to reduce the parasitaemia in animals treated at the dose of 100 mg/kg b.w. (intraperitoneally, two times daily for 3 days) and of 150 mg/kg b.w. (per os, two times daily for 3 days), respectively. The reduction of parasitaemia was, however, statistically significant (p=0.002) only in case of treatment with Terminalia avicennioides.     

Antidepressant activity of aqueous extracts of Curcuma longa in mice

Curcuma longa (turmeric) is a well-known indigenous herbal medicine. The aqueous extracts, when administered orally to the mice from 140 to 560 mg/kg for 14 days, were able to elicit dose-dependent relation of immobility reduction in the tail suspension test and the forced swimming test in mice. The effects of the extracts at the dose of 560 mg/kg were more potent than that of reference antidepressant fluoxetine. The extracts, at the dose of 140 mg/kg or above for 14 days, significantly inhibited the monoamine oxidize A (MAO) activity in mouse whole brain at a dose-dependent manner, however, oral administration of the extract only at a dose of 560 mg/kg produced observable MAO B inhibitory activity in animal brain. Fluoxetine showed only a tendency to inhibit MAO A and B activity in animal brain in the study. Neither the extracts of C. longa nor fluoxetine, at the doses tested, produced significant effects on locomotor activity. These results demonstrated that C. longa had specifically antidepressant effects in vivo. The activity of C. longa in antidepression may mediated in part through MAO A inhibition in mouse brain.     

银杏叶提取物的紫外谱线组图谱的研究

采用指纹谱线组合技术测绘银杏叶提取物(EGB761)的紫外谱线组图谱,具有特征性强、重现性好的优点,可应用于中药原药材、提取物以及制剂的真伪鉴别与质量控制。初步确定EGB761的标准紫外谱线组为:λmax(nm):水溶液(C=0.02 mg/ml)265.00±1.00,201.00±1.00;无水乙醇溶液(C=0.02 mg/ml)267.00±1.00,210.00±1.00;氯仿溶液(C=0.4 mg/ml)241.00±1.00;石油醚溶液(C=0.4 mg/ml)211.00±1.00。     

大黄素对大鼠肝移植后肝细胞凋亡的影响

目的:探讨大黄素对大鼠肝移植后肝细胞凋亡的影响。方法:建立LEW→BN大鼠肝移植动物模型,随机分为A,B,C,D4组,每组6只动物,术后每天分别腹腔注射生理盐水0.5mL.d^-1、环孢素A(CsA)10.0mg.kg^-1.d^-1、大黄素50.0mg.kg^-1.d^-1、大黄素50.0mg.kg^-1.d^-1+CsA10.0mg.kg^-1.d^-1,连续用药8d,停药7d后处死动物取肝脏组织观察排斥活动指数(RAI)和肝细胞凋亡指数(AI)。结果:A,B,C,D组RAI分别是(7.67±0.98),(5.17±0.40),(5.83±0.75),(3.83±0.75),AI分别是(35.83±2.32),(15.83±1.33),(16.50±2.35),(11.50±1.05)。B,C,D组的RAI,AI较A组均明显降低(P<0.01),而D组较B,C组也均有明显降低(P<0.05),B,C组间差别均无意义。结论:大黄素能有效减少大鼠移植肝术后肝细胞凋亡,抑制排斥反应,且和CsA有协调作用。