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1.
Cultured cells from Micropolyspora faeni-sensitized donors can adoptively transfer murine experimental hypersensitivity pneumonitis (EHP). We sought to determine
the location of transferred cells in recipient animals, the influence of the origin of the cultured cells, and the effect
of specific intratracheal challenge. We labeled cultured sensitized spleen or lung-associated lymph node (LALN) cells with
CFDA-SE, a cytoplasmic stain, before transfer to naive recipients, which were sacrificed 1 h, 1 day, or 4 days thereafter.
We also transferred labeled cultured spleen cells to recipients that were challenged with intratracheal M. faeni and sacrificed 4 days later (MF). Controls were recipients of M. faeni-sensitized and cultured cells challenged with intratracheal normal saline (NS) and recipients of ovalbumin (OVA)-sensitized
cells cultured with M. faeni and challenged with intratracheal M. faeni (OVA). The number and proportion of cells that were stained were determined in dispersed spleen, peripheral and lung-associated
lymph nodes, and lung parenchyma. The extent of the pulmonary inflammatory response was measured by determining the proportion
of microscopic fields that were abnormal and the total number of dispersed pulmonary cells. CFDA-SE stained cells uniformly,
and stained cells could be detected in recipients for up to 7 days after transfer. CFDA-SE treatment (0.5 μM) did not affect
the ability of cells to transfer EHP adoptively. Transferred cells could be detected easily in lung, lung-associated and peripheral
lymph nodes, blood, and spleen. Transferred cells localized to the lung at 1 h but then rapidly decreased with no difference
between labeled cells from spleen and LALN. After intratracheal M. faeni challenge, there was no difference in the proportion of labeled cells in the lung among any of the groups (MF, NS, or OVA).
There was an increase in the number of lung cells in the MF group compared with the control (NS and OVA) groups. We conclude
that cells capable of transfer are transiently (1 h) trapped in the lung but are much decreased in the lung by four days after
transfer. After intratracheal antigen challenge of recipients, there is a substantial increase in the number of pulmonary
cells in animals exhibiting adoptive EHP but not in the control groups. Transferred cells responsible for EHP are increased
in the lungs of animals with adoptive EHP.
Accepted for publication: 30 June 1997 相似文献
2.
Detection of Interstitial Pneumonitis in Patients with Rheumatoid Arthritis by Measuring Circulating Levels of KL-6, a Human MUC1 Mucin 总被引:1,自引:0,他引:1
T. Oyama N. Kohno A. Yokoyama Y. Hirasawa K. Hiwada H. Oyama Y. Okuda K. Takasugi 《Lung》1997,175(6):379-385
In rheumatoid arthritis (RA), interstitial pneumonitis is one of the major extraarticular complications that worsens a patient's
prognosis. KL-6, a human MUC1 mucin, has been reported to be a sensitive serum marker for activity of interstitial pneumonitis.
We investigated the clinical significance of serum KL-6 level in patients with RA. Serum levels of KL-6 and RA-associated
inflammatory markers were evaluated in 177 RA patients. The diagnosis of active interstitial pneumonitis was made by clinical
symptoms, pulmonary function tests, chest X-ray film, and high resolution CT. Serum KL-6 was increased in 8 of 9 (88.9%) RA
patients with active interstitial pneumonitis but in only 1 of 168 (0.6%) RA patients without active interstitial pneumonitis.
No significant correlation was found between KL-6 level and conventional clinical parameters. In RA, abnormal elevation of
serum KL-6 strongly indicates the complication of active interstitial pneumonitis.
Accepted for publication: 11 February 1997 相似文献
3.
4.
5.
Shimizudani N Murata H Kojo S Adachi Y Keino H Tsuchida F Sumida M Kawamata M Sumida T Matsuoka T 《Lung》2001,179(1):31-41
Chronic eosinophilic pneumonitis (CEP) is characterized by longstanding respiratory symptoms accompanied by a massive pulmonary
eosinophil infiltration. T lymphocytes in bronchoalveolar lavage (BAL) from patients with chronic eosinophilic pneumonitis
are considered to recognize unknown antigens. To analyze the pathogenesis of CEP, we examined the T cell receptor (TCR) repertoire
and T cell clonotype of BAL lymphocytes and peripheral blood lymphocytes (PBLs) in a 66-year-old woman patient with CEP. The
expression of TCR BV gene was analyzed by the family PCR method using specific primers for 20 TCR BV genes and BC gene. The
clonotype of BAL and peripheral T cells was examined by the PCR-single-strand conformation polymorphism (SSCP) method. Functional
sequences of some T cell clones were also carried out. A TCR repertoire of BAL T cells was heterogeneous as well as PBLs.
However, SSCP analysis showed that distinct T cell clonotypes were detected in BAL T cells, TCR BV3, BV4, BV6, BV8, BV9, BV14,
and BV18-positive T cell clones especially, expanded clonally in BAL from the patient. Sequencing analysis showed that GVD,
LGG, RDXS, and SSG amino acid sequence motif were found in the CDR3 in lung-specific T cells. BAL-specific T cell clones accumulated
in the patient with CEP. Thus, we can conclude that BAL T cells are induced by the antigen-driven stimulation and these cells
might play a crucial role in the generation of CEP.
Accepted for publication: 27 February 2001 相似文献
6.
HDL and Vitamin E in Plasma and the Expression of SR-BI on Lung Cells during Rat Perinatal Development 总被引:1,自引:0,他引:1
Vitamin E is the most important lipophilic antioxidant, and beneficial effects on oxidant-caused injuries have been reported.
Neonates are at high risk of oxidative injury in the lung and other organs because of a low vitamin E concentration, but the
optimal timing of the application, a safe application form, and the optimal dosage of vitamin E are not known at present.
We recently showed that alveolar type II cells take up vitamin E preferentially from high-density lipoprotein (HDL), probably
by means of the candidate HDL receptor, scavenger receptor class B type I (SR-BI; Kolleck et al. Free Rad Biol Med 27;882-890,
1999). Therefore, both the HDL-bound vitamin E in plasma and the expression of SR-BI on alveolar type II cells may determine
the supply of the cells with vitamin E.
We show here that the plasma level of vitamin E, total and HDL cholesterol, and the ratio of vitamin E to polyunsaturated
fatty acids and to total fatty acids decrease during fetal rat development, reaching the minimum at the postconceptual day
21 (day −1). These parameters increase thereafter to about the same levels as in adult rats. SR-BI is not detectable until
day −1 on fetal lung cells, but the expression during the postnatal phase follows the same pattern as the plasma lipid constituents.
We conclude that the ability of alveolar type II cells to take up vitamin E develops perinatally in mature neonates. This
aspect also has to be considered when the optimal timing of supplementation for the protection of preterm neonates with vitamin
E against oxidative lung injury is established.
Accepted for publication: 6 April 2000 相似文献
7.
Serum levels of surfactant protein A (SP-A) were studied in 237 healthy subjects in relation to sex, age, and smoking habits.
SP-A values in male smokers were significantly higher than those in male nonsmokers (p < 0.001). The amount of cigarette smoking did not correlate significantly with SP-A values, however. SP-A values in young
nonsmoking males and females were somewhat lower than those in older, but without significant difference. No significant difference
in values was found between the sexes. We conclude that (1) smoking increases serum levels of SP-A, and (2) SP-A serum levels
are not affected by age and sex.
Accepted for publication: 6 March 1998 相似文献
8.
Antioxidant Function of Ambroxol in Mononuclear and Polymorphonuclear Cells in Vitro 总被引:34,自引:0,他引:34
This study quantifies the antioxidant function of ambroxol (2-amino-3,5-dibromo-N-[trans-4-hydroxycyclohexyl]benzylamine) in vitro. Polymorphonuclear cells (PMN) and mononuclear cells were isolated from the blood
of healthy volunteers (n= 46) to determine reactive oxygen species (ROS) by luminol-enhanced chemiluminescence. Ambroxol or the controls N-acetylcysteine (NAC), nacystelyn (NAL), glutathione (GSH), superoxide dismutase (SOD), catalase, and the combination of SOD/catalase
were incubated for 1 or 2 h with zymosan-activated cells in vitro using concentrations ranging from 10−6 to 10−3 mol/liter. Reduction of ROS-mediated luminescence was similar within the cell types. Ambroxol (10−4 mol/liter) reduced ROS about 75% (1-h incubation) and 98% (2-h incubation), respectively (p < 0.001). SOD and SOD/catalase, but not the H2O2-catalyzing substances (NAC, NAL, GSH, and catalase), reduced cellular ROS. This indicates that inflammatory cells predominantly
generate O−
2, which can be scavenged by ambroxol. The antioxidant function of ambroxol with increasing incubation time suggests additional
cellular antiinflammatory properties of this substance. Our results indicate that good antioxidant function of ambroxol is
related mainly to direct scavenger function of reactive oxygen metabolites such as O−
2. However, an antioxidative effect of ambroxol may also be associated with the reduction of prooxidative metabolism in inflammatory
cells. Concluding from this observation, and because of the well known high affinity of ambroxol for lung tissue, ambroxol
may be an alternative in antioxidant augmentation therapy, particularly in pulmonary diseases characterized by an overburden
of toxic oxygen metabolites.
Accepted for publication: 5 December 1996 相似文献
9.
Fibroblasts play a crucial role in progressive lung fibrosis, acting not only as target cells but also as effector cells.
To clarify these functions in sarcoidosis, lung fibroblasts from Japanese sarcoid patients were studied for their proliferative
capacity and cytokine productivity. Fibroblasts were cultured from transbronchial lung biopsy specimens from seven patients
with sarcoidosis. As a comparison, fibroblasts from open lung biopsy specimens of four patients with idiopathic pulmonary
fibrosis (IPF) were studied. For controls, fibroblasts were cultured from specimens of normal resected lung tissue of five
patients with localized lung cancer. The proliferative activity of cultured fibroblasts from patients with sarcoidosis was
highest among the three groups (p < 0.05). However, the proliferative capacity in all groups was suppressed when fibroblasts were cultured with interleukin-1β
(IL-1β). No significant differences were noted in the degree of inhibition among the three groups. Addition of interferon-γ
(IFN-γ) also resulted in inhibition of fibroblast growth in all groups, but the degree of inhibition was significantly greater
in both the sarcoid and IPF groups than in controls (p < 0.05). The amount of interleukin-6 (IL-6) in the culture supernatants from sarcoid fibroblasts cocultured with IL-1β was
significantly higher than in controls. Sarcoid fibroblasts are not only proliferatively active but also possess effector cell
function to produce cytokines. IL-6 may enhance the immunologic reaction to sarcoidosis and cause the disease to become chronic.
IFN-γ suppresses proliferation of sarcoid fibroblasts and may prevent fibrotic changes of the lungs in the Japanese sarcoid
patients.
Accepted for publication: 4 June 1997 相似文献
10.
J. Marks-Konczalik A. Gillissen M. Jaworska S. Löseke B. Voss A. Fisseler-Eckhoff I. Schmitz G. Schultze-Werninghaus 《Lung》1998,176(3):165-180
Superoxide dismutases play an important protective role in the lung defense against the pro-oxidative effect of fibrous dusts
(e.g. crocidolite fibers). Particularly crocidolite, but also other asbestos fibers, are known to induce cellular antioxidant
defense. Although rockwool, a man-made fiber made from rock, is used widely for insulation purposes, its effects on the superoxide
dismutases in bronchoepithelial cells have not been investigated. Thus, the purpose of this study was to determine whether
human bronchoepithelial cells (BEAS 2B) respond to rockwool fibers (115-4 experimental rockwool fiber) by induction of MnSOD
mRNA and an increase of MnSOD activity levels. The results were compared with BEAS 2B cells exposed to silica (α-quartz: DQ12;
SiO2) and UICC (Union Internationale Contre le Cancer) crocidolite (concentrations of all dusts: 0, 2, 5, 10, 25, 50 μg/cm2= 0, 2.4, 6, 12, 30, 60 μg/ml; 24-h exposure) as control fibers. Scanning electron microscopy confirmed close dust cell contact
under all experimental settings. Very low MnSOD mRNA baseline levels rose significantly (p < 0.001) in BEAS 2B cells exposed to all three dusts at 2 μg/cm2. However, at >25 μg/cm2 MnSOD mRNA levels in silica- and crocidolite- but not in rockwool-exposed cells decreased. Slight (no significance) increases
of MnSOD activity were observed which decreased at higher dust (>5 μg/cm2) concentrations. These results suggest that: (1) like crocidolite and silica, rockwool accelerates MnSOD gene expression
in bronchoepithelial cells; (2) an increase of MnSOD mRNA levels is not accompanied by MnSOD activity elevation; (3) in contrast
to rockwool, high concentrations (≥25 μg/cm2) of crocidolite and silica reduced MnSOD activity and MnSOD mRNA levels. Because oxidants (H2O2) and crocidolite fibers were shown to reduce SOD activity, lack of active MnSOD protein may be caused by inactivation on
a post-translational level. Furthermore, the decline of MnSOD mRNA and MnSOD activity levels coincides with increasing cytotoxicity.
In conclusion, rockwool was demonstrated to induce MnSOD gene expression, perhaps because of its pro-oxidative effect in bronchoepithelial
cells. In contrast to crocidolite and silica, rockwool fibers are not cytotoxic in this experimental setting.
Accepted for publication: 21 August 1997 相似文献
11.
N. Shijubo Y. Itoh T. Yamaguchi F. Sugaya M. Hirasawa T. Yamada T. Kawai S. Abe 《Lung》1999,177(1):45-52
Clara cell 10-kDa protein (CC10), the predominant product from nonciliated cells in the epithelial lining of bronchioles
(Clara cells), has been shown to have immunomodulatory and antiinflammatory activity and may play a role in controlling airway
inflammation. This study was designed to measure serum CC10 concentrations in healthy and asthmatic nonsmokers. Serum CC10
concentrations in asthmatic nonsmokers were significantly lower than in healthy nonsmokers. Asthmatic patients with a long
duration of the disease (≥10 years) had significantly lower serum CC10 levels than those with a short duration of the disease
(<10 years). There was no significant difference in serum CC10 levels in asthmatic patients between the time of the asthmatic
attack and the stable condition. Serum CC10 levels may reflect decreased production of CC10 caused by remodeling of the small
airways in asthma.
Accepted for publication: 30 July 1998 相似文献
12.
Because some authors have reported high rates of failure in performing the single breath N2 (SBN2) test in rural areas, the present study aimed at evaluating its acceptability in a female population, unfamiliar with lung
function testing, in a rural area of northeastern France. Two hundred ninety-eight women from a rural area volunteered for
a preventive medicine examination (91.6% of those invited); four of them were excluded for clinical reasons, and six (2%)
were unable to perform spirometry. The protocol included completion of a questionnaire, spirometry with a bronchial reactivity
test, skin prick test, and the SBN2 test utilizing a computerized assembly. Although failures caused by the apparatus were few (n= 7, 2.4%) 96 of 281 women (34.1%) were unable to produce two valid SBN2 tests in a series of six attempts. Compared with the group who succeeded in the test (n= 185), women who failed were older and had a higher prevalence of bronchial hyperresponsiveness. Logistic regression confirmed
the independent association of these two variables with an inability to perform. We conclude that in a female population completely
unfamiliar with lung function testing the SBN2 test has a high rate of failure associated with higher age and the presence of bronchial hyperresponsiveness.
Accepted for publication: 17 November 1998 相似文献
13.
The investigation of peripheral blood lymphocyte (PBL) subpopulations is of interest in a wide variety of inflammatory diseases.
Since the number of circulating lymphocytes has been shown to be affected by smoking habits, it seems useful to know how PBL
subpopulations are influenced. We therefore determined percentages and absolute numbers of a wide range of PBL subpopulations
in smokers (n= 14) and nonsmokers (n= 14). PBLs were obtained from healthy volunteers and analyzed by flow cytometry using antibodies for the detection of CD3,
CD4, CD8, CD19, CD56, CD57, CD45RO, CD45RA, α/β and γ/δ T cell receptor epitopes. With the exception of CD3+ cells, no differences between smokers and nonsmokers were found regarding percentages of PBL subpopulations. Smokers were
found to have higher absolute numbers of PBLs in the following subpopulations compared with nonsmokers: CD3+, CD4+, CD3+α/β+, CD45RO+/CD4+, and CD45RA+/CD4+. Cytotoxic lymphocytes, natural killer cells, and B cells did not differ in number between smokers and nonsmokers. There
was likewise no difference in the number of the CD8+α/β+ and all cells bearing the γ/δ T cell receptor. Smoking increased the number of T cells and mainly CD4+ PBLs. The smoking habits of healthy control groups should therefore be taken into account when comparing lymphocyte subpopulations
in different diseases.
Accepted for publication: 13 February 1997 相似文献
14.
Surfactant protein A (SP-A) increases the resistance of surfactant to inhibition by plasma and other proteins. In a previous
study we found that a monoclonal anti-SP-A antibody (R 5) increased the sensitivity of surfactant to inhibition by fibrinogen
in vivo and in vitro. SP-A has been shown to stimulate microbial phagocytosis and killing by alveolar macrophages. We hypothesized
that using R 5 to inactivate SP-A in an animal model mimicking congenital group B streptococcal (GBS) pneumonia might result
in increased bacterial proliferation and a deterioration in lung function. Newborn near term rabbits were delivered by Cesarean
section, anesthetized, tracheotomized, and ventilated for 5 h in a plethysmograph system allowing measurement of dynamic lung-thorax
compliance. Postnatally the animals received one intratracheal injection (5 ml/kg) of R 5, nonspecific IgG, or normal saline.
At 30 min all animals received a standard dose of an encapsulated GBS strain by intratracheal injection. The number of bacteria
(mean log10 CFU/g lung ± S.D.; CFU = colony forming unit) was evaluated in lung homogenates. Histologic lung sections were judged by
light microscopy. Bacterial proliferation was similar in rabbits treated with the monoclonal antibody (9.33 ± 0.39; n= 14) and in control animals receiving saline (9.16 ± 0.35; n= 14) or nonspecific IgG (9.26 ± 0.31; n= 11). No significant differences were noted on the histologic analysis or in measurements of lung function. We conclude that
intratracheal instillation of a monoclonal anti-SP-A antibody did not increase bacterial proliferation in GBS-infected newborn
rabbits. These findings suggest that SP-A does not play an important role in protection against encapsulated GBS strains in
the neonatal period.
Accepted for publication: 20 June 1997 相似文献
15.
Synthesis and release of 1,25-dihydroxycholecalciferol (1,25-(OH)2D2) by alveolar macrophages (AM) have been shown to be increased in granulomatous lung disease. ICAM-1 plays a major part in
leukocyte homing to sites of chronic inflammation, which is a crucial step during the inflammatory response. Whether 1,25-(OH)2D2 alters the ICAM-1 expression of AM in humans has not been studied. Bronchoalveolar lavage (BAL) was performed in 12 healthy
volunteers, in 13 patients with sarcoidosis (active disease n= 8, inactive disease n= 5), and in 9 patients with chronic bronchitis. AM were incubated with different concentrations of 1,25-(OH)2D2 (10−11 to 10−6 M) with and without priming with interferon-γ (IFN-γ) and with and without preincubation with 10−8 M dexamethasone. In addition, the metabolites of vitamin D, 24,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol,
were used. The AM expression of ICAM-1 (cELISA) and the release of tumor necrosis factor-α (TNF-α) (bioassay) by AM were determined.
In healthy volunteers the ICAM-1 expression on AM was significantly and dose-dependently increased by 1,25-(OH)2D2, but not by 24,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol. Priming with IFN-γ resulted in an additive effect.
Preincubation with dexamethasone inhibited ICAM-1 expression. Addition of 1,25-(OH)2D2 after inhibition by dexamethasone increased ICAM-1 expression significantly. TNF-α secretion of AM from healthy volunteers
was significantly reduced by 1,25-(OH)2D2. In sarcoidosis patients ICAM-1 expression was significantly higher compared with healthy volunteers. Incubation with 1,25-(OH)2D2 resulted in a further significant increase of ICAM-1 expression. TNF-α secretion of AM was increased compared with healthy
volunteers. 1,25-(OH)2D2 reduced TNF-α secretion; however, this difference was not significant. 1,25-(OH)2D2 has an immunomodulating effect on human AM both in healthy volunteers and in sarcoidosis patients with enhanced expression
of ICAM-1. It may serve as an autocrine mediator in inflammatory lung disease.
Accepted for publication: 5 November 1998 相似文献
16.
Angiotensin-converting Enzyme Activity by Canine Pulmonary Microvascular and Central Pulmonary Artery Endothelial Cells Exposed to Hypoxia 总被引:1,自引:0,他引:1
To compare the amount of angiotensin-converting enzyme (ACE) activity in pulmonary artery endothelial cells from different
sites and to examine the effect of severe hypoxia (less than 1% of O2 in 5% CO2 and 95% N2) on the ACE activity expressed by these cells, endothelial cells were harvested and cultured from canine main pulmonary artery
by scraping the luminal surface of the artery and from canine pulmonary artery microvessels by infusing chilled buffer with
microcarrier beads and 0.02% ethylenediamine tetraacetic acid (EDTA). ACE activity in cell lysates and culture medium was
evaluated by fluorometric assay with hippuryl-L-histidyl-L-leucine as a substrate.
ACE activity in cell lysates and postculture medium of pulmonary microvascular endothelial cells (PMVEC) was higher than in
cell lysates and culture medium of central pulmonary artery endothelial cells (PAEC). However, hypoxia suppressed cellular
ACE activity in both PAEC and PMVEC. The degree of suppression of ACE activity by hypoxia, which was determined as (ACE activity
in normoxia − ACE activity in hypoxia)/ACE activity in normoxia × 100(%), was larger in PMVEC than in PAEC.
The pulmonary microvasculature may be a greater source of ACE than central pulmonary artery, and the ACE activity of pulmonary
microvascular endothelial cells seem to be sensitive to hypoxia, although the small diameter of the vessels improves conditions
for interaction of blood-borne substance with endothelial enzymes.
Accepted for publication 10 July 2000 相似文献
17.
Spirometry prediction equations obtained from middle-age adults, when extrapolated for the elderly, may lead to inaccurate
interpretations. The purpose of this study was to determine prediction equations for forced vital capacity (FVC) and forced
expiratory volume (FEV1) in the Greek elderly population. Spirometry prediction equations for normal FVC and FEV1 have been derived from tests on 71 healthy persons (38 men, 33 women) aged older than 60 years (range, 65–85 years), nonsmokers,
white race, urban population using techniques and equipment that meet American Thoracic Society recommendations. Regression
analysis using age, height, and weight as independent variables was used to provide prediction equations and values for both
sexes. The FVC age coefficient in this healthy group was about 47.19 mL/y for elderly men and 34.27 mL/y for elderly women,
and the FEV1 age coefficient was about 52.8 mL/y for elderly men and 46.4 mL/y for elderly women . Values from this study predicted equations
were compared with those from some of the most commonly used sources of spirometry predicted equations. The FVC and FEV1 predicted values were found to be of less mean square error than that of other compared studies. Higher correlation is between
FVC and FEV1 predicted values by the present model and FVC and FEV1 observed values in both sexes. The higher correlation between FVC and FEV1 predicted and observed from this study allows the use of our model for predicting in a rather reliable way the FVC and FEV1 for elderly Greek individuals.
Accepted for publication: 25 April 2000 相似文献
18.
The effect of administering prostaglandin E1 (PGE1) on the extent of monocrotaline (MCT)-induced pulmonary hypertension and cytokine production [interleukins (IL) 1 and 6 and
tumor necrosis factor (TNF)] by macrophages during MCT induction of pulmonary hypertension was studied. Right ventricle/left
ventricle plus septum weight ratios (RV/LV + S) were used as an index of the development of pulmonary hypertension. Administering
PGE1 at a dose of 0.2 mg/kg/day for 4 weeks reduced significantly the RV/LV + S ratio from 0.428 ± 0.070 to 0.243 ± 0.059 (p < 0.01) and decreased the production of these cytokines: IL-1, from 4.675 ± 3.558 to 1.800 ± 0.722 units; IL-6, from 0.322
± 0.121 to 0.060 ± 0.039 units; and TNF, from 0.578 ± 0.369 to 0.004 ± 0.004 units. In another series of experiments, a significant
reduction of the RV/LV + S ratio was noted for only 1 week when we administered PGE1 immediately after the injection of MCT. We confirmed that histopathologic improvements of lungs were noted by administering
0.2 mg/kg PGE1 for 4 weeks. In another experiment, PGE1 at a concentration of 2 μg/ml suppressed a rise in the cytosolic Ca2+ concentration of lipopolysaccharide-stimulated peritoneal macrophages of rats in vitro, suggesting that PGE1 suppressed cytokine production by macrophages through the suppression of the Ca2+ influx. These results suggest that administering PGE1 may be effective in the treatment of some forms of pulmonary hypertension in humans.
Accepted for publication: 20 August 1998 相似文献
19.
M. Hosp A. M. Elliott J. G. Raynes A. G. Mwinga N. Luo R. Zangerle J. O. M. Pobee H. Wachter M. P. Dierich K. P. W. J. McAdam D. Fuchs 《Lung》1997,175(4):265-275
Neopterin is a biochemical marker for the activation of the cell-mediated immune system. We measured neopterin, β2-microglobulin, and acute phase proteins in 31 HIV-seropositive and -seronegative Zambian patients with tuberculosis, using
stored sera that had been obtained at the beginning and at end of antituberculosis treatment. In both HIV-seropositive and
-seronegative patients neopterin and acute phase proteins were elevated when tuberculosis was initially diagnosed and fell
during treatment. In contrast, the mean β2-microglobulin level increased during antituberculous therapy in the HIV-seropositive group. Serum neopterin levels at diagnosis
were correlated with other parameters of disease activity (fever, anemia, and weight loss). In both groups, patients with
persistently elevated neopterin levels at the end of treatment were more likely to suffer relapse of tuberculosis or other
adverse health events in the subsequent follow-up period. Neopterin can be used to monitor the response to antituberculous
therapy in both HIV-seropositive and -seronegative patients and may have a prognostic value for the patients' wellbeing in
the follow-up period.
Accepted for publication: 13 December 1996 相似文献
20.
Evaluation of Cyfra 21-1: a potential tumor marker for non-small cell lung carcinomas 总被引:16,自引:0,他引:16
Cyfra 21-1 is a tumor marker based on the determination of water-soluble cytokeratin 19 which is secreted by normal or malignantly
transformed epithelial cells. It is suggested to be a valuable marker in patients with non-small cell lung carcinoma (NSCLC).
A prospective clinical study was conducted to investigate the value of Cyfra 21-1 for diagnosis, determination of subtypes,
staging, and evaluation of therapy response in patients with lung carcinoma (Ca). Sixty-nine patients (mean age: 60.9 ± 9.2
years, M/F:12.8) treated between 1994 and 1998 inclusive, and 13 healthy smokers (mean age:50.9 ± 4.8 years, M/F:1.6) constituted
the study group and control group, respectively. Venous blood samples (10 ml) were obtained from all subjects. Posttreatment
blood samples were also obtained from 14 NSCLC patients. Cyfra 21-1 levels (cutoff value 3.3 ng/ml) were determined by ELSA-Cyfra
21-1 kit (CIS bio international, France) through immunoradiometric assay (IRMA). Cyfra 21-1 levels did not differ between
smoking and non-smoking subjects within each group (p > 0.05). Cyfra 21-1 was significantly elevated in lung Ca cases irrespective of the cell type (p < 0.05). It was significantly elevated in squamous cell and adenocarcinoma varieties with the most prominent elevation in
squamous cell type (p < 0.05). In lung Ca, the specificity and sensitivity of Cyfra 21-1 was 92.3% and 52.2%, respectively. Sensitivity was 65.5%
for NSCLC, 70.5% for squamous cell, and 45.5% for adenocarcinoma varieties, with highest sensitivity rates in Stage IIIA +
IIIB (87.5%) and Stage IV (75%) of squamous cell lung Ca. Cyfra 21-1 level was significantly decreased after treatment in
NSCLC patients (n 14) (p < 0.01). Cyfra 21-1 is a tumor marker that helps to establish the diagnosis and differentiation of cell type and evaluation
of response to therapy in patients with NSCLC.
Accepted for publication: 3 April 2001 相似文献