缺铁性贫血对大鼠甲状腺功能的影响

目的 研究缺铁性贫血对大鼠甲状腺功能的影响,以期为铁缺乏地区的碘缺乏病防治工作提供新的线索和思路.方法 选择健康SPF-VAF级初断乳SD雄性大鼠30只,按体质量随机分为对照组(饲料平均含铁量为93.3mg/kg)和缺铁性贫血组(饲料平均含铁量为9.6 mg/kg),每组15只.喂养6周后,测定大鼠体质量和甲状腺质量,...     

缺铁性贫血对大鼠生殖机能及仔鼠生长发育的影响

为研究缺铁性贫血 (IDA)对大鼠的生殖机能及对仔鼠生长发育影响 ,将 4周龄Wistar雌性大鼠随机分为二组 ,实验组喂低铁基础饲料 ,对照组喂在基础饲料中添加铁的对照饲料。 14周后与正常雄鼠合笼交配 ,观察仔鼠的生长发育情况。对母鼠及仔鼠血清微量元素Fe、Zn、Cu、Mn、Se进行测定。结果显示 ,IDA组母鼠窝仔均数、活产率、活胎数及幼仔 4日存活率均低于对照组 (P <0 0 1) ;IDA组仔鼠出生体重低、生长迟缓、毛发稀疏 ,具典型贫血临床特征。;IDA组仔鼠血液指标检测 :血红蛋白、红细胞压积均低于对照组 (P <0 0 0 0 1)。母鼠及仔鼠血清Fe、Cu低于对照组 (P <0 0 0 0 1)。结论认为缺铁性贫血可降低大鼠的生殖机能 .严重地影响了仔鼠的正常生长发育 ,并导致仔鼠体内其它微量元素代谢失调。     

当归阿胶口服液改善大鼠缺铁性贫血的效果评价

目的评价当归阿胶口服液改善缺铁性贫血的效果。方法选雌性断乳大鼠80只,以低铁饲料喂养造模后选血红蛋白(Hb)<100 g/L的个体,按Hb水平分层随机分为低铁对照组和3个试验组,每组12只大鼠。设试验剂量为5.00、2.50、1.25 ml/kg.bw,低铁对照组给予去离子水。每日1次经口灌胃给样,试验周期42 d。试验终末测定大鼠Hb和红细胞内游离原卟啉(FEP)含量。结果与低铁对照组相比较,各剂量组大鼠Hb含量显著上升,FEP含量显著降低。结论当归阿胶口服液有改善缺铁性贫血的作用。     

卵转铁蛋白铁制剂对缺铁性贫血小鼠的影响

目的研究卵转铁蛋白铁制剂对缺铁性贫血小鼠的影响。方法从鸡蛋蛋清中分离纯化得到卵转铁蛋白,与Fe3+结合,制得饱和卵转铁蛋白铁制剂。采用健康小鼠60只,随机分成正常对照组(n=12)和模型组(n=48)。模型组喂低铁饲料,4w后根据血红蛋白含量(Hb)、红细胞数(RBC)、红细胞压积(HCT)及体重水平,确定36只缺铁贫血小鼠,并随机分成3组:缺铁贫血模型组、试验Ⅰ组(卵转铁蛋白铁制剂组)、试验Ⅱ组(右旋糖酐铁组)。对照组喂常规饲料,缺铁贫血模型组、试验Ⅰ组、试验Ⅱ组喂低铁饲料。试验Ⅰ组、试验Ⅱ组灌胃受试物,对照组灌胃自来水,缺铁贫血模型组灌胃去离子水,连续3w。分别于小鼠试验前(0w)、灌胃受试物前(4w)和灌胃受试物3w后眼眶采血,测定Hb、RBC、HCT和体重,末次采血后取小鼠心、肝、脾、肺、肾标本,并计算脏器重量与体重的比值。结果试验Ⅰ组Hb、RBC和HCT明显高于缺铁贫血模型组,差异均极显著(P0.01),试验Ⅱ组Hb、RBC和HCT高于缺铁贫血模型组,Hb差别有极显著性(P0.01),RBC和HCT差异显著(P0.05),恢复效果试验Ⅰ组优于试验Ⅱ组。缺铁贫血模型组小鼠心脏系数和脾脏系数均高于其余的各组。结论卵转铁蛋白铁制剂用于恢复缺铁性贫血小鼠有显著效果。     

乙二胺四乙酸铁钠口服液治疗小儿缺铁性贫血及对血清微量元素的影响

目的:探讨乙二胺四乙酸铁钠(NaFeEDTA)口服液治疗婴幼儿缺铁性贫血的疗效及其对血清微量元素的影响。方法:对101例缺铁性贫血(IDA)患儿给予NaFeEDTA口服液0.5mg·kg-1·d-1,连服8周,治疗前、后分别测定血常规和微量元素,并与正常儿童进行对照。结果:用药8周后93.0%的IDA患儿血红蛋白(Hb)恢复至正常水平(P<0.01);治疗前IDA患儿血清铁、锌、铜均较正常儿童低(P<0.05),治疗8周后血清铁、锌、铜均较治疗前明显升高(P<0.05),血清钙、镁变化不明显。结论:NaFeEDTA口服液治疗婴幼儿IDA临床效果好,可显著升高血清铁、锌、铜水平。     

荣脉软胶囊改善缺铁性贫血的动物实验研究

目的 观察荣脉软胶囊对实验性大鼠改善缺铁性贫血的作用。方法 选用刚断乳雌性SD大鼠80只，用低铁饲料喂养，剪尾放血构建低铁性贫血大鼠模型，按血红蛋白（Hb）水平随机分为低铁对照组（色拉油）、阳性对照组（硫酸亚铁）和3个受试物组，受试物组（低、中、高剂量组，分别为0.2 g/kg、0.4 g/kg和1.2 g/kg），每组10只。每天经口灌胃1次，连续30 d。试验结束测定大鼠Hb、红细胞内游离原卟啉含量和红细胞压积。结果 试验后中、高剂量组大鼠Hb含量分别为(103.0±12.4) g/L、(111.0±6.3) g/L，与低铁对照组(86.6±9.6) g/L相比明显升高（P均<0.05）；红细胞压积分别为(37.9±3.8)%、(39.8±2.5)%，与低铁对照组(33.1±3.3)%相比明显升高（P<0.05）。高剂量组大鼠红细胞内游离原卟啉含量为(320.6±72.6)μg/L，与低铁对照组(437.0±93.2)μg/L相比明显降低（P<0.05）。结论 荣脉软胶囊对实验性大鼠的缺铁性贫血具有改善作用。     

雌性大鼠缺铁性贫血模型的建立及相关指标观察研究

目的:建立缺铁性贫血动物模型并观察动物的一般情况及各项贫血相关指标的变化,为分析孕期缺铁对胎儿健康的影响提供理论依据。方法:选取雌性Wistar大鼠32只,随机分为对照组和实验组,每组各16只,置于不锈钢鼠笼喂养。对照组以含铁元素216.0 mg/kg的普通饲料喂养;实验组以含铁元素10.0 mg/kg的低铁饲料喂养。两组均自由采食,饮用去离子水。每周定期采血1次,测定血红蛋白(Hb)浓度、红细胞比容(PCV)、红细胞平均容积(MCV)和红细胞平均血红蛋白含量(MCH);实验结束后检测血浆和肝脏组织中的铁蛋白(Fn)、胰岛素样生长因子-1(IGF-1)、促红细胞生成素(EPO)水平。结果:实验组大鼠的一般体态发生明显变化,表现为毛皮发黄、粗糙、趾腹发白、运动迟缓和体重增加缓慢。1个月后,实验组Hb、PCV、MCV、MCH水平均明显低于对照组,两组比较,差异有统计学意义(P<0.05);红细胞体积分布宽度(RDW)、血浆和肝脏组织匀浆EPO水平均显著增加,两组比较,差异有统计学意义(P<0.05);实验组大鼠血浆Fn和IGF-1水平均降低,两者比较,差异有统计学意义(P<0.05)。结论:雌性大鼠以低铁饲料喂养可以成功建立缺铁性贫血的动物模型,其相关指标都有明显变化,为分析孕期缺铁对胎儿健康的影响提供理论依据。     

全谷物稻米对正常大鼠糖脂相关代谢组学的影响

目的 探讨全谷物稻米与普通饲料及精制稻米相比,对正常大鼠血糖、血脂及相关代谢组学的影响.方法 36只SPF级雄性SD大鼠,按初始体重和空腹血糖分为3组,分别饲喂AIN-93 G饲料(对照组)及采用精制米粉或糙米粉配制的饲料(45％精米组、45％糙米组),共喂养18周.每周监测进食量、体重,实验末期取腹主动脉血测定血糖、...     

乙二胺四乙酸铁钠强化酱油改善贫血效果观察

本研究比较了NaFeEDTA强化酱油、硫酸亚铁 (FeSO4)强化酱油对IDA改善效果。将 30 0名IDA学生分为对照组、NaFeEDTA强化酱油组Fe 5mg (人·日 )、FeSO4强化酱油组Fe5mg (人·日 ) ,比较新型铁强化剂 (NaFeEDTA)与传统铁剂 (FeSO4)对缺铁性贫血的改善作用。结果表明 ,对照组各项检验指标干预前后没有显著差异。其它各试验组呈现出较为一致的变化 ,表现为血红蛋白、血清铁、血清铁蛋白含量的显著性增加和原卟啉、总铁结合力、转铁蛋白的显著性下降。试验结果提示 ,乙二胺四乙酸铁钠与传统铁剂 (硫酸盐铁 )强化酱油均有改善学生贫血的作用 ,并且NaFeEDTA组的贫血改善率和Hb恢复水平优于FeSO4组。     

铁缺乏对母鼠及仔鼠体内微量元素含量的影响

目的　对缺铁性贫血 (IDA)母鼠及仔鼠生长发育进行研究 ,观察IDA对母代和仔代大鼠血清及组织中微量元素含量的变化。方法　将 4周龄Wistar雌性大鼠喂低铁饲料建立IDA模型。母鼠 14周龄后合笼交配 ,对断乳母鼠、出生 2天和 4 0天仔鼠血清、肝、脑、脾组织进行微量元素Fe、Zn、Cu、Mn、Se含量测定。结果　 (1)血清微量元素的含量 :IDA母鼠血清Fe、Cu、Mn含量低于对照组 (P <0 0 1,P <0 0 5 )。IDA仔鼠血清Fe、Cu低于对照组 (P <0 0 1)。(2 )肝、脑、脾组织中微量元素含量 :①IDA母鼠肝、脑、脾组织中Fe含量均低于对照组 ;而Zn、Cu、Mn、Se含量无显著性差异。②IDA仔鼠肝、脑、脾组织Fe含量与对照组相比有显著性差异。出生 2天仔鼠肝组织中Cu、Mn含量低于对照组 ;而出生 4 0天仔鼠脑组织Cu、Mn含量高于对照组。结论　缺铁性贫血不仅可造成母代与仔代大鼠体内铁的缺乏 ,还可引起机体内其他微量元素代谢的改变。     

螺旋藻对缺铁性贫血的恢复实验

选纯种Ｗｉｓｔａｒ大鼠，用低铁饮食法复制缺铁性贫血（ＩＤＡ）模型，之后，用螺旋藻（ＳＰ）进行恢复试验。以硫酸亚铁为阳性对照组，以低铁饲料为阴性对照组，按ＳＰ含量由小到大分为３个实验组即实验１组（含ＳＰ１％）、实验２组（含ＳＰ８％）及实验３组（含ＳＰ１５％）。经过４周的恢复试验，结果：实验２组和实验３组贫血恢复速度最快，其次是实验１组。实验２组和实验３组体重高于阳性对照组，实验１组体重与阳性对照组接近，阳性对照组体重高于阴性对照组。提示：（１）单纯性缺铁可影响大鼠生长，致大鼠体重增长缓慢；（２）螺旋藻似具有加速大鼠体重增长的作用；（３）螺旋藻用于恢复大鼠ＩＤＡ效果显著，而且用量有一个最适宜的范围．     

乳母补铁对预防婴儿缺铁性贫血的观察

[目的 ]　探讨预防婴儿缺铁性贫血的有效方式。 [方法 ]　随机分预防组和对照组 ,血红蛋白正常的预防组乳母 ,产后 4个月口服血红素铁 (益气维血颗粒 )每日 1包 (10克 ) ,共 30天。两组婴儿生后 6个月做手指末梢血Hb、MCV、MCH、MCHC测定。 [结果 ]　预防组婴儿无 1例贫血 ,对照组婴儿 5 3 .13 %发生贫血。两组婴儿Hb差异非常显著(P <0 .0 0 1)。 [结论 ]　乳母补铁可有效预防婴儿缺铁性贫血的发生     

周日补铁治疗儿童缺铁性贫血疗效观察

〖目的〗 观察每周一次补铁治疗缺铁性贫血（ＩＤＡ）的疗效。〖方法〗 采用设未治疗的病例对照组和正常对照组的方法,对５０例ＩＤＡ患儿每周一次性补充元素铁２ｍｇ／ｋｇ,共１２周。在治疗前、中、后观察Ｈｂ,ＺＰＰ,ＳＦ三项指标的变化。〖结果〗 治疗组治疗１２周后Ｈｂ、ＳＦ均极显著性升高（Ｐ〈０．０１）,ＺＰＰ极显著性降低（Ｐ〈０．０１）,病例对照组各指标各有改善,不能达到正常水平。〖结论〗 ７日补铁法对     

Iron absorption from brown rice/brown rice-based meal and milled rice/milled rice-based meal

Background Milled rice is the staple food among Filipinos and is mostly consumed three times a day. Rice as a source of iron could therefore have an important role in the existing 37% prevalence of iron-deficiency anemia in the country. Previous iron absorption studies in Filipinos from rice and rice-based meals were carried out on milled rice but no research was done on brown rice of the same variety. This leads to the hypothesis that brown rice may be better than milled rice in terms of iron content.

Objective To determine iron absorption from brown rice and brown rice-based meal, and from milled rice and milled rice-based meal of the same variety.

Methods The rice variety used in the study was F₂ seeds of PSB Rc72H. Iron absorption from brown/milled rice and brown/milled rice-based meals was determined in 12 healthy human subjects from the incorporation of radioisotopes of iron into erythrocytes 14 days after administration of the labeled rice/rice-based meals. The above samples were also analyzed for nutrient content, including dietary fiber, and iron.

Results The iron content of brown rice was significantly higher (1.1 ± 0.1 mg/100 g) than that of milled rice (0.6 ± 0.1 mg/100 g). Brown rice has significantly greater amounts of total dietary fiber (5.4 ± 0.4%) than milled rice (1.7 ± 0.2%; P < 0.05). Both tannic acid and phytic acid contents in brown rice (56.9 ± 3.2 mg/100 g and 290.1 ± 18.0 mg/100 g, respectively) were significantly higher than those of milled rice (21.3 ± 2.3 mg/100 g and 84.0 ± 12.4 mg/100 g, respectively; P<0.05). The amount of iron absorbed from brown rice (0.13 ± 0.02 mg) did not differ significantly from that from milled rice (0.14 ± 0.02 mg). However, the amount from brown rice-based meal (0.36 ± 0.04 mg) differed significantly from that from brown rice (P<0.05) as well as that from milled rice-based meal (0.35 ± 0.03 mg) from that from milled rice (P<0.05). Moreover, brown rice-based meal did not differ significantly from milled rice-based meal (P<0.05).

Conclusion Iron absorbed from milled rice and brown rice did not differ significantly, as well as that from brown rice-based meal and milled rice-based meal. Differences in iron absorbed from brown/milled rice and brown/milled rice-based meals may be due to the iron content of the test foods and the presence of iron enhancers in the meal (e.g. fish, vegetables and citrus fruit).     

轻度缺铁性贫血对儿童身体作功能力的影响

本文研究了130名轻度缺铁性贫血和正常的7岁儿童的身体作功能力。分别在治疗前、后30天及60天时采手指血分析Hb、FEP、FEP/Hb、SF;同时测定身体作功能力指标(PWC_(170)、R·PWC_(170)、W·HR、Vo_2max)。发现轻度缺铁性贫血儿童的各测定指标均与正常儿童有显著差异,但经硫酸亚铁(300mg/d)治疗60天后,各测定指标均恢复到正常水平。本文研究结果证明了轻度缺铁性贫血可使儿童身体作功能力明显降低。     

Iron absorption in young Indian women: the interaction of iron status with the influence of tea and ascorbic acid

BACKGROUND: Ascorbic acid (AA) enhances and tea inhibits iron absorption. It is unclear whether iron status influences the magnitude of this effect. OBJECTIVE: We evaluated the influence of the iron status of young women on iron absorption from a rice meal with or without added tea or AA. DESIGN: Two stable-isotope iron absorption studies were made in 2 groups of 10 subjects with iron deficiency anemia (IDA) and 10 subjects who were iron replete (control subjects). In study 1, the reference rice meal was fed alone or with 1 or 2 cups of black tea. In study 2, the reference meal was fed alone or with AA (molar ratio to iron, 2:1 or 4:1). Iron absorption was measured by the erythrocyte incorporation of (57)Fe and (58)Fe labels at 14 d. RESULTS: Mean fractional iron absorption from the reference rice meal was approximately 2.5 times as great in the IDA group as in the control group (P < 0.05). The consumption of 1 or 2 cups of tea decreased iron absorption in the control subjects by 49% (P < 0.05) or 66% (P < 0.01), respectively, and in the IDA group by 59% or 67% (P < 0.001 for both), respectively. AA (molar ratio to iron, 2:1 or 4:1) increased iron absorption by 270% or 343%, respectively, in control subjects and by 291% or 350%, respectively, in subjects with IDA (P < 0.001). CONCLUSIONS: The inhibitory effect of tea and the enhancing effect of AA on iron absorption were similar in the 2 groups. Overall differences in iron absorption in the 2 groups, however, continued to be dictated by iron status.     

妊娠中期母亲铁营养与3~5月龄婴儿缺铁性贫血关系的研究

目的 研究孕中期母亲铁营养状况与3~5月龄婴儿缺铁性贫血(iron-deficiency anemia,IDA)的关系,为预防儿童IDA的发生提供科学依据。方法 测定妊娠中期母亲和脐带血血清铁蛋白(serum ferritin,SF)和血常规,随访至婴儿3~5月龄,测定婴儿血常规,对血红蛋白(hemoglobin,Hb)低于105 g/L的婴儿采集静脉血测定SF。比较妊娠中期母亲铁营养状况、脐带血SF和3～5月龄婴儿IDA的关系。结果 1) 3~5月婴儿IDA的发生率在孕中期铁缺乏组和正常组分别是12.9%和1.5%(χ²=4.62,P<0.05);2) 脐带血SF<75μg/L的发生率在孕中期铁缺乏组和正常组分别是25.8%和4.6%(χ²=9.6,P<0.05);3)3~5月龄婴儿IDA的发生率在脐带血SF<75 μg/L组是31.6%,脐带血SF≥75 μg/L组是2.8%(χ²=16.21,P<0.01)。结论 妊娠中期母亲轻度缺铁可减少胎儿期铁贮备,与早期婴儿IDA呈正相关。     

Anaemia and iron deficiency anaemia among aboriginal schoolchildren in rural Peninsular Malaysia: an update on a continuing problem

A cross-sectional study to determine the current prevalence of anaemia and iron deficiency anaemia (IDA) and to investigate the possible risk factors for IDA was carried out on 241 aboriginal schoolchildren (120 boys, 121 girls) aged 7-12 years and living in remote areas in Pos Betau, Pahang, Malaysia. Haemoglobin (Hb) level was measured and serum iron status was assessed by serum ferritin (SF), serum iron (SI) and total iron binding capacity measurements. Socioeconomic data were collected using pre-tested questionnaires. All children were screened for intestinal parasitic infections. Overall, 48.5% (95% CI 42.3-54.8) of children were anaemic (Hb<12g/dl). The prevalence of IDA was 34% (95% CI 28.3-40.2), which accounted for 70.1% of the anaemia cases. The prevalence of IDA was significantly higher in females than males. Low levels of mothers' education and low household income were identified as risk factors for IDA. Severe trichuriasis also found to be associated with low SF and SI. Logistic regression confirmed low levels of mothers' education and gender as significant risk factors for IDA. Improvement of socioeconomic status and health education together with periodic mass deworming should be included in public health strategies for the control and prevention of anaemia and IDA in this population.     

重组人乳铁蛋白对缺铁性贫血大鼠铁营养状况的改善作用

目的研究从转基因牛乳中分离纯化的重组人乳铁蛋白(rhLf)对缺铁性贫血(IDA)大鼠铁营养状况是否具有改善作用。方法雌性断乳Wistar大鼠喂饲低铁饲料建立IDA模型,按造模结束时Hb和体重随机分为6组,即模型对照组(A组),0.375、0.75和2.25g/kg BW rhLf组(B、C、D组),0.011g/kg BW乳酸亚铁组(E组),0.375g/kg BW rhLf与0.011g/kg BW乳酸亚铁联合补充组(F组),每组12只,每天一次性灌胃给予受试物,连续8周。检测指标包括动物一般情况、体重变化、Hb和RBC、游离原卟啉(FEP)、血清铁(SI)、总铁结合力(TIBC)、运铁蛋白饱和度(TS)、血清铁蛋白(SF)、血清Cu和血清Zn。结果与A组相比,E、F组大鼠一般情况明显改善,体重、RBC、Hb、SI、TS和SF显著增加,FEP、TIBC和血清Zn降低,机体铁营养状态基本恢复正常;D组上述指标较A组同样有显著性差异,但改善程度低于E、F组,动物仍处于缺铁状态;C组RBC较A组显著增加,FEP和血清Zn显著降低,其他指标无差异;B组仅血清Zn显著低于A组,其他指标无差异。E、F两组比较时,F组SI、TS和SF均显著高于E组,TIBC低于E组。结论单纯补充rhLf对IDA大鼠铁营养状况具有一定程度的改善作用,但较乳酸亚铁弱;乳酸亚铁与rhLf联合应用时改善机体铁营养状况的作用强于单纯补充乳酸亚铁或rhLf,同时可减轻亚铁离子导致的不良反应。