白细胞介素-4-590C/T基因多态性与牙周炎易感性的Meta分析

目的 评价白细胞介素-4-590C/T(IL-4-590C/T)基因多态性与牙周炎易感性之间的关系.方法 计算机检索PubMed、Embase、ScienceDirect和Web of Science、CBM、CNKI、WanFang、VIP数据库,搜集国内外有关基因多态性与牙周炎相关性的病例对照研究,经文献筛选、资料提取和质量评价后,采用R 3.3.1软件进行Meta分析,按牙周病分类及人群种族进行亚组分析.结果 Meta分析结果 显示,总体:IL-4-590C/T等位基因频率及各基因型频率与牙周炎间无显著关联:(T vs C:OR=1.19,95%CI=0.811.76,P=0.37).亚组:高加索人种对于携带CC基因型而言,携带TT基因更具牙周炎易感性(TT vs CC:OR=1.75,95%CI=1.10~2.78,P=0.02),差异具有统计学意义,而该基因型在亚洲人种未见显著差异(P=0.63);在慢性牙周炎与侵袭性牙周炎中,均未发现IL-4-590C/T等位基因频率及基因型频率与二者存在易感性(以T vs C.为例,慢性牙周炎:OR=1.20,95%CI=0.75~1.90,P=0.45;侵袭性牙周炎:OR=1.17,95%CI=0.77~1.78,P=0.45).结论 IL-4-590C/T基因多态性与高加索人牙周炎易感性间有一定相关性,与亚洲人牙周炎易感性无关.     

IL-1β基因3954C＞T位点多态性与侵袭性牙周病易感性的Meta分析

目的:通过Meta分析进一步探究IL-1β基因3954C＞T位点多态性改变与侵袭性牙周炎的相关性.方法:检索PubMed,Embase,CNKI和万方数据库中有关IL-1β基因3954C＞T位点多态性与侵袭性牙周炎易感性相关文献,以OR值和95％的可信区间为效应指标,应用STATA 11.0软件进行Meta分析,并对发表偏倚及敏感性分析进行检验.结果:纳入22个病例对照研究,共计965例侵袭性牙周炎患者和1234例对照,Meta分析结果显示,总人群中,IL-1β基因3954C＞T位点多态性与牙周病风险之间没有显着关联(T vs.C:OR=0.966,95 ％CI=0.696～1.341:CT vs.CC:OR=0.936,95％CI=0.761～1.151;TT vs.CC:OR=0.892,95％CI=0.464～1.715;CT+TT vs.CC:OR=1.026,95％CI=0.795-1.323;TT vs.CC+CT:OR=0.864,95％CI=0.436、1.713).相应的亚组分析未发现IL-1β基因3954C＞T位点多态性和侵袭性牙周炎易感性无显著相关性.结论:IL-1β基因3954C＞T位点多态性多态性可能与侵袭性牙周炎的发生无关.     

白细胞介素-4基因-590C／T多态性与慢性牙周炎的相关性研究

目的研究白细胞介素-4（interleukin-4,IL-4）基因-590C／T多态性与慢性牙周炎易感性的关系。方法采用病例对照试验设计,聚合酶链反应一限制性内切酶片段长度多态性基因分析方法,比较104例慢性牙周炎患者（慢性牙周炎组）和106例牙周健康者（健康对照组）IL-4基因-590位点基因型和等位基因分布特点。结果IL-4基因-590位点C、T等位基因频率（X2=0．771,P=0．380）及基因型频率（X2=1．904,P=0．386）在两组间分布差异无统计学意义。结论IL-4基因-590位点的多态性与汉族人群慢性牙周炎的易感性无明显相关性。     

转化生长因子-β1和白细胞介素-10单核苷酸多态性与复发性口腔溃疡易感性的研究

目的 研究转化生长因子-β1（TGF-β1）-509T/C位点与白细胞介素-10（IL-10）-1082A/G位点的单核苷酸多态性与复发性口腔溃疡（RAU）易感性的相关性。方法 采用限制性片段长度多态性-聚合酶链式反应（RFLPPCR）RFLPPCR）法和序列特异性引物-聚合酶链式反应（SSP-PCR）法对138例RAU患者和124例健康对照者进行TGF-β1-509位点与IL-10-1082位点的单核苷酸多态性的检测分析,用比值比（OR）和95%可信区间（95%CI）估计相对危险度。结果 TGF-β1-509位点与IL-10-1082位点在基因型频率与等位基因频率的分布上,病例组与正常对照组之间均存在明显差异（P<0.05）。TGF-β1-509位点基因型CT（OR=1.231,95%CI=0.702~2.160）与TT（OR=2.482,95%CI= 1.250~4.927）为高风险基因型,T等位基因为高风险等位基因（OR=1.465,95%CI=1.036~2.074）。IL-10-1082位点基因型AG（OR=1.391,95%CI=0.808~2.396）与GG（OR=4.165,95%CI=1.944~8.924）为高风险基因型,G等位基因为高风险等位基因（OR=2.134,95%CI=1.474~3.089）。结论 TGF-β1-509位点与IL-10-1082位点是RAU患者的易感基因位点。TGF-β1-509位点携带T等位基因患RAU的风险性是携带C等位基因者的1.465倍。IL-10-1082位点携带G等位基因患RAU的风险性是携带A等位基因者的2.134倍。     

IL-6-572基因多态性与侵袭性牙周炎易感性的相关性研究

目的 研究白细胞介素-6(interleukin-6,IL-6)-572位点基因多态性与侵袭性牙周炎易感性的关系.方法 采用病例对照试验设计,从广东汉族人群中选择83例侵袭性牙周炎(aggressive periodontitis,AgP)患者(AgP组)及79例牙周健康者(对照组),采用聚合酶链反应—限制性内切酶片段长度多态性分析方法对IL-6-572位点基因多态性进行检测,分析组间基因型频率及等位基因分布的差异.结果 IL-6基因启动子区-572位点G/C基因型在AgP组、对照组中的分布频率差异有统计学意义(x2=13.710,P=0.001).AgP组与对照组相比,G、C等位基因频率分布差异有统计学意义(x2=13.213,P<0.001),G等位基因相对于C等位基因:OR值为2.988,95%CI:1.634~5.465.结论 IL-6-572 G/C位点的基因多态性同中国广东汉族人群侵袭性牙周炎患病易感性可能存在相关关系,IL-6-572 G等位基因可能是广东汉族人群AgP遗传易感性的高风险因素.     

MTHFR基因C677T位点多态性与口腔癌易感性的Meta分析

[摘要]目的：运用Meta分析方法研究MTHFR基因C677T位点多态性与口腔癌易感性的发生风险。方法：检索VIP,CNKI和PubMed数据库中有关MTHFR基因C677T位点多态性与口腔癌易感性关联研究的文献,以OR值和95％的可信区间为效应指标,应用STATA11．0软件进行Meta分析,并对发表偏倚及敏感性分析进行检验。结果：纳入7个病例对照研究,共计1318例口腔癌患者和1817例正常对照,Meta分析结果显示,总人群中,MTHFR基因C677T多态性与口腔癌风险之间没有显着关联（TVS．C：95％CI=0．688—1．272,P=0．672,Pheterogeneity=0．001;TI＂VS．CC：95％CI=0．638-1．763,P=0．821,Pheterogeneity=0．059：CTVS．CC：95％CI=0．581—1．282,P=0．465,Pheterogeneity=0．002;（CT＋TY）VS．CC：95％CI=0．666-1．345,P=0．761,Pheterogeneity〈0．001;TI＇VS．（CC＋CT）：95％CI=0．709~1．247,P=0．669,Pheterogeneity=0．126）。在针对种族和对照人群来源设计的亚组分析中,来源于医院对照的亚组分析模型显示基因突变可能会降低发生口腔癌的风险。结论：MTHFR基因C677T位点多态性突变可能降低口腔癌的发病风险。     

白细胞介素13基因-1112C/T多态性与慢性牙周炎的相关性研究

目的 探讨白细胞介素13(interleukin-13,IL-13)基因启动子区-1112C/T多态性与慢性牙周炎易感性的相关性.方法 采用病例对照试验设计,慢性牙周炎患者110例(慢性牙周炎组)和牙周健康者106例(健康对照组),聚合酶链反应-限制性片段长度多态性方法检测两组患者IL-13基因-1112位点基因型和等位基因分布特点.结果 IL-13基因-1112位点C、T等位基因频率(x2=0.886,P=0.347)及基因型频率(x2=1.982,P=0.371)在两组间分布差异无统计学意义.结论 IL-13基因-1112位点的多态性与汉族人群慢性牙周炎的易感性无明显相关性.     

IFN-γ+874 A/T位点和IL-2-330 T/G位点的单核苷酸多态性与复发性口腔溃疡易感性的关系

目的：：研究IFN-γ+874A/T位点与IL-2-330T/G位点的单核苷酸多态性( SNP)与复发性口腔溃疡( RAU)易感性的关系。方法：采用ELISA方法检测138例RAU组患者和124例正常对照组中IFN-γ及IL-2的血清含量,并用SSP-PCR和RFLP-PCR对全血进行IFN-γ+874位点与IL-2-330位点的单核苷酸多态性的检测分析。结果：RAU组患者血清中IFN-γ及IL-2水平均显著低于正常对照组(P<0.05)。 IFN-γ+874位点在基因型频率与等位基因频率的分布2组间差异有显著性(P<0.05)。 IFN-γ+874位点基因型AA的OR值=9.964为易感基因型,A等位基因为易感等位基因, OR值=3.801。 IL-2-330位点在基因型频率与等位基因频率的分布2组间差异无显著性(P>0.05)。 IFN-γ+874位点中,携带A等位基因患者患复发性口腔溃疡的风险是携带T等位基因者的3.801倍。结论：IFN-γ+874位点中携带A的等位基因与RAU易感性相关,IL-2-330位点与RAU的易感性无关。     

白细胞介素-6基因-572C/G多态性与慢性牙周炎的相关性研究

目的研究白细胞介素-6（interleuk in-6,IL-6）基因启动子区域-572C/G位点基因多态性与慢性牙周炎易感性的关系。方法采用病例对照试验设计,轻中度牙周炎组87例,重度牙周炎组72例,健康对照组90例,收集颊粘膜拭子,使用聚合酶链反应—限制性内切酶片段长度多态性基因分析的方法,检测各组IL-6-572位点基因型和等位基因分布。结果 IL-6基因-572C/G位点G等位基因的检出率在健康组是14.4%,轻中度牙周炎组是14.4%,重度牙周炎组是20.1%。等位基因频率在患者和健康者之间无统计学差异（P=0.287）。CC基因型在各组的分布分别是健康组73.3%,轻中度牙周炎组71.3%,重度牙周炎组63.9%,各组之间差异无统计学意义（P=0.308）。结论 IL-6基因-572位点多态性与汉族人群慢性牙周炎的易感性无明显相关。     

IL-1B＋3954C/T和IL-1B-511C/T基因多态性和种植体周围炎的相关性研究

目的：探讨研究种白介素1（IL-1）基因＋3954和-511位点单核酸多态性和种植体周围炎的关系以及临床意义。方法：采用病例对照实验设计,选取种植体炎患者以及对照组各50名,应用聚合酶链反应—限制性内切酶片段长度多态性基因分析方法研究两组IL-1B基因＋3954位点和-511位点基因型和等位基因分布特点,并探讨其与种植体周围炎的相关性。结果：IL-1B基因＋3954位点CC、CT基因型检出率为93%和7%,无TT基因型,两种基因型和C、T等位基因频率在组间分布差异无统计学意义（P〉0.05）;IL-1B基因-511位点主要以CT为主,检出率为58%,CC、CT基因型检出率都为21%。结论：排除吸烟,牙周炎病史等影响因素后,IL-1B基因＋3954位点和-511位点的多态性与种植体周围炎炎的易感性无关。     

Association Between Interleukin‐4 Gene −590 C/T, −33 C/T,and 70‐Base‐Pair Polymorphisms and Periodontitis Susceptibility: A Meta‐Analysis

Background: Several studies have investigated the association between interleukin (IL)‐4 gene ?590 C/T, ?33 C/T, or 70–base pair (70‐bp) polymorphisms and periodontitis susceptibility but with conflicting results. Hence, a meta‐analysis was conducted to explore whether these polymorphisms are associated with periodontitis susceptibility. Methods: A comprehensive literature search was conducted of PubMed, Embase, Scopus, ScienceDirect, and Web of Science up to April 5, 2014. After the eligible studies were identified, data were extracted and quality‐assessed before performing the meta‐analysis. Results: The meta‐analysis included 23 eligible case‐control studies from 11 articles involving 12 studies of the ?590 C/T polymorphism (1,220 cases and 2,039 controls), five of the ?33 C/T polymorphism (715 cases and 967 controls), and four of the 70‐bp polymorphism (426 cases and 506 controls). The meta‐analysis showed that none of these IL‐4 gene polymorphisms were significantly associated with periodontitis susceptibility in all study participants. However, subgroup analysis showed that the IL‐4 ?590 T allele (odds ratio [OR] = 1.2, 95% confidence interval [CI] = 1.02 to 1.42, P = 0.03) and TT genotype (OR = 1.68, 95% CI = 1.05 to 2.67, P = 0.03) were associated with periodontitis in whites. Conclusions: Based on current evidence, the IL‐4 ?33 C/T and 70‐bp polymorphisms were not associated with an increased risk of periodontitis. However, the IL‐4 ?590 T allele and TT genotype were associated with increased risk of periodontitis in whites.     

Single nucleotide polymorphisms of pattern recognition receptors and chronic periodontitis

Sahingur SE, Xia X‐J, Gunsolley J, Schenkein HA, Genco RJ, De Nardin E. Single nucleotide polymorphisms of pattern recognition receptors and chronic periodontitis. J Periodont Res 2011; 46: 184–192. © 2010 John Wiley & Sons A/S Background and Objective: Periodontitis is a multifactorial disease influenced partly by genetics. Activation of pattern recognition receptors (PRRs) can lead to the up‐regulation of inflammatory pathways, resulting in periodontal tissue destruction. Hence, functional polymorphisms located in PRRs can explain differences in host susceptibility to periodontitis. This study investigated single nucleotide polymorphisms of PRRs including toll‐like receptor (TLR)2 (G2408A), TLR4 (A896G), TLR9 (T1486C), TLR9 (T1237C) and CD14 (C260T) in patients with chronic periodontitis and in periodontally healthy subjects. Methods: One‐hundred and fourteen patients with chronic periodontitis and 77 periodontally healthy subjects were genotyped using TaqMan® allelic discrimination assays. Fisher’s exact test and chi‐square analyses were performed to compare genotype and allele frequencies. Results: The frequency of subjects with the CC genotype of CD14 (C260T) (24.6% in the chronic periodontitis group vs. 13% in the periodontally healthy group) and those expressing the T allele of CD14 (C260T) (CT and TT) (75.4% in the chronic periodontitis group vs. 87% in the periodontally healthy group) was statistically different among groups (p = 0.04). Homozygocity for the C allele of the CD14 (C260T) polymorphism (CC) was associated with a two‐‐fold increased susceptibility to periodontitis (p = 0.04; odds ratio, 2.49; 95% confidence interval, 1.06–6.26). Individuals with the CC genotype of TLR9 (T1486C) (14.9% in the chronic periodontitis group vs. 28.6% in the periodontally healthy group) and those expressing the T allele of TLR9 (T1486C) (CT and TT) (85.1% in the chronic periodontitis group vs. 71.4% in the periodontally healthy group) were also significantly differently distributed between groups without adjustment (p = 0.03). Further analysis of nonsmokers revealed a significant difference in the distribution of genotypes between groups for TLR9 (T1486C; p = 0.017) and CD14 (C260T; p = 0.03), polymorphisms again without adjustment. Conclusion: The CC genotype of CD14 (C260T) is related to susceptibility to chronic periodontitis in Caucasians. In addition, differences observed in the distribution of TLR9 (T1486C) genotypes between groups warrant further investigation.     

The association between interleukin polymorphism and recurrent aphthous stomatitis: A meta-analysis

ObjectiveTo assess the association between interleukin gene polymorphism and recurrent aphthous stomatitis (RAS).DesignsTwo electronic databases, PubMed and Embase, were utilized to assemble potentially relevant studies meeting the inclusion criteria. A meta-analysis was conducted using Revman 5.3 software (London, UK), and the pooled odds ratio (OR) and 95% confidence interval (CI) were then used to evaluate the strength of the relationship between the gene polymorphisms of IL-1beta(−511C/T), IL-1beta(+3954C/T), IL-6(−174G/C) and IL-10(−1082G/A) and the risk of RAS.ResultsTen studies were included in the final meta-analysis, with 884 cases and 1104 controls participating. The results demonstrated that the polymorphism of IL-1beta(−511C/T) significantly increased the probability of the development of RAS in Europeans. (T vs. C: OR = 1.35, 95%CI = 1.09–1.67; CC vs. CT + TT: OR = 1.77, 95%CI = 1.24–2.53; CC vs. TT: OR = 1.86, 95%CI = 1.18–2.95). Furthermore, the C allele in IL-1beta(+3954C/T) was determined to be related to the risk of RAS in Americans (C vs. T: OR = 1.52, 95%CI = 1.07–2.17) and the presence of the C gene was considered a risk variant (CC + CT vs. TT: OR = 1.46, 95%CI = 1.01–2.11), but no relationship was found between the polymorphism of IL-10(−1082G/A) and the risk of RAS.ConclusionsThe meta-analysis suggested that the mutation of IL-1beta(−511C/T) in Europe and IL-1beta(+3954C/T) in America tend to increase the risk of RAS, but the polymorphism of IL-10(−1082G/A) appears to have no association with RAS risk in America. Further study is required to confirm the above conclusions.     

转化生长因子β1基因-509位点多态性与重度慢性牙周炎易感性的关系

目的 探讨转化生长因子β1(transforming growth factor beta-1,TGF-β1)基因-509位点多态性与重度慢性牙周炎易感性的关系,以期从基因水平探讨牙周炎发病的遗传学机制.方法 用聚合酶链反应-限制性片段长度多态性方法检测102例重度慢性牙周炎患者(牙周炎组)和102名健康对照者(健康对照组)的TGF-β1基因-509位点,比较两组间此位点基因型分布和等位基因频率的差异.结果 TGF-β1基因-509位点CC、CT、TT基因型在牙周炎组和健康对照组的分布频率分别为44.1%(45/102)、47.1%(48/102)、8.8%(9/102)和29.4%(30/102)、51.0%(52/102)、19.6%(20/102),两组人群基因型分布频率差异有统计学意义(P＜0.05);等位基因C、T在牙周炎组和健康对照组分布频率分别为67.6%(138/204)、32.4%(66/204)和54.9%(112/204)、45.1%(92/204),两组人群的等位基因分布频率差异亦有统计学意义(P＜0.05),C等位基因携带者患重度慢性牙周炎的风险是T等位基因的1.718倍(OR=1.718,95%CI:1.148～2.569).结论 TGF-β1基因-509位点多态性与重度慢性牙周炎的发病具有相关性,C等位基因可能是重度慢性牙周炎的遗传易感基因.     

Quantitative Assessment of the Associations Between Interleukin‐8 Polymorphisms and Periodontitis Susceptibility

Background: This review assesses the associations of interleukin‐8 gene (IL‐8) ?251A/T (rs4073) and ?845T/C (rs2227532) polymorphisms with susceptibility to periodontitis. Methods: Several electronic databases were searched for eligible articles. Twelve studies involving 2,233 cases and 2,655 controls were retrieved and analyzed. Odds ratios (ORs) along with 95% confidence intervals (CIs) were calculated to assess the strength of relationship between the IL‐8 polymorphisms and periodontitis risk. Results: No significant association was found for IL‐8 ?251A/T polymorphism with periodontitis in the overall analysis and stratification by periodontitis type and smoking status. Subgroup analysis by ethnicity revealed that ?251A/T T allele and TT genotype were associated with decreased risk of periodontitis in a Brazilian mixed population (T allele versus A allele: OR 0.80, 95% CI 0.68 to 0.94, P_{heterogeneity} = 0.30; TT versus AA: OR 0.65, 95% CI 0.46 to 0.93, P_{heterogeneity} = 0.39; TT versus AA/AT: OR 0.58, 95% CI 0.35 to 0.98, P_{heterogeneity} = 0.01). In addition, ?251A/T T allele was associated with increased periodontitis risk in Asians. Pooled estimates showed that the ?845T/C polymorphism was associated with periodontitis susceptibility in overall analysis and the chronic periodontitis subgroup. In addition, marginal associations were observed between ?845T/C polymorphism and periodontitis in a Brazilian mixed population. Moreover, this association was also confirmed to be significant in Brazilian non‐smokers. Conclusion: This meta‐analysis indicated that both IL‐8 ?251A/T and ?845T/C polymorphisms may be involved in the development of periodontitis in a Brazilian mixed population, whereas the ?251A/T allele T appeared to be a risk factor for periodontitis in Asians.     

The association of interleukin-4 haplotypes with chronic periodontitis in a Czech population

BACKGROUND: Cytokine gene polymorphisms are known to influence the susceptibility and disease course of many chronic disorders. Recently, interleukin (IL)-4 gene polymorphisms were associated with aggressive periodontitis. The aim of this study was to test differences in the distribution of the IL-4 alleles, genotypes, and haplotypes between patients with chronic periodontitis (CP) and healthy controls in a Czech population. METHODS: The association study was conducted using an age- and smoking status-matched case-control design in patients with CP (n = 194) and healthy controls (n = 158) using the polymerase chain reaction-restriction fragment length polymorphism methods for the -590C/T, -33C/T, and intron 3 variable number tandem repeat (VNTR) variants of the IL-4 gene. RESULTS: No significant differences between patients and controls were found in allele and genotype frequencies of all three polymorphisms. Nevertheless, complex analysis revealed significant differences in haplotype frequencies between the groups (P = 0.005). The haplotype T(-590)/T(-33)/allele 2 VNTR (70 base pairs)(2) of the IL-4 gene was significantly more frequent in patients with CP than in controls (17.0% versus 11.0%; odds ratio = 1.85; 95% confidence interval: 1.19 to 2.87). CONCLUSION: The three polymorphisms in the IL-4 gene act in a cooperative fashion and suggest that the high-production IL-4 haplotype was associated with an increased risk for CP in the Czech population.