孕妇乙肝免疫球蛋白被动免疫阻断HBV母婴垂直传播作用机理的研究

目的 :研究HBV阳性孕妇孕期应用乙肝免疫球蛋白 (HBIG)预防HBV宫内感染的作用机理。方法 :将 78例乙肝表面抗原 (HBsAg)阳性孕妇分为两组 :预防组 30例 ,于孕 2 8、32、36周肌肉注射HBIG 3次 ,每次 2 0 0IU ;对照组 4 8例 ,只随访查体不用药。检测母儿血清乙肝标志物 (HBVM)和细胞因子IFN γ ,IL 12 ,IL 6水平用双抗夹心酶联免疫吸附法 (DAS ELISA) ,测定HBVDNA含量用荧光定量PCR(FQ PCR)技术。结果 :78例HB sAg阳性孕妇分娩的新生儿宫内感染 10例 ,宫内感染率为 12 .82 % .HBIG预防组孕妇的胎儿HBV感染率显著低于对照组 (P <0 .0 5 ) ;预防组新生儿脐血清抗 HBs检出率显著高于对照组 (P <0 .0 0 1) ;预防组孕妇血清中IFN γ ,IL 12水平显著高于对照组 (P <0 .0 5 ) ,IL 6水平、HBVDNA含量则显著低于对照组 (P <0 .0 5 )。结论 :孕妇HBIG被动免疫可有效阻断HBV母婴垂直传播。     

乙型肝炎病毒感染孕妇IL-18对Th1/Th2细胞免疫平衡的作用与胎儿宫内感染相关性研究

目的:探讨乙型肝炎病毒(HBV)感染孕妇外周血白细胞介素-18(IL-18)水平对Th1/Th2细胞免疫的平衡作用与胎儿宫内感染的相关性。方法:选择2006年1月至2006年10月在本院分娩孕产妇101例,合并HBV感染、肝功能正常,单胎、足月妊娠。检测新生儿脐血HBV-DNA及HBsAg,诊断胎儿宫内感染21例为研究组,无宫内感染80例为对照组。用双抗夹心酶联免疫吸附法(DAS-ELISA)检测孕妇外周血清IL-18、IFN-γ(γ-干扰素)、IL-4的水平。结果:(1)研究组孕妇外周血IL-18及IFN-γ明显低于对照组,而IL-4水平明显高于对照组,两组差异有统计学意义(P<0.05,P<0.01,P<0.05);研究组IFN-γ/IL-4比值较对照组明显降低,差异有统计学意义(P<0.01)。(2)两组孕妇外周血IL-18与IFN-γ均呈显著正相关、与IL-4水平均呈显著负相关、与IFN-γ/IL-4比值均呈显著正相关。结论:HBV宫内感染与孕妇Thl型特异性反应降低以及Th2型特异性反应增强有关,Th1/Th2免疫平衡失调可能是胎儿HBV宫内感染的机制之一;HBV宫内感染与孕妇外周血IL-18分泌下降有关,IL-18水平下调可能是HBV宫内感染的危险因素之一。     

细菌性阴道病和早产者宫颈分泌物IL-1βIL-6含量变化的相关性

目的　探讨宫颈分泌物细胞因子 (IL - 1β和IL - 6 )含量与细菌性阴道病 (BV)、早产的关系。 方法　选择孕 32～ 34周无宫缩而胎膜完整的有BV孕妇 2 7例和无BV孕妇 30例 ,以及胎膜完整的未足月临产孕妇 2 5例和足月临产孕妇 2 6例 ,进行宫颈分泌物取样 ,分别采用酶联免疫吸附法和放射免疫法测定宫颈分泌物IL -1β、IL - 6的含量。 结果　宫颈分泌物IL - 1β的含量在BV组和未足月临产组均高于无BV组 (P <0 0 5 ) ,而未足月临产组与足月临产组间无差异 (P >0 0 5 ) ;宫颈分泌物IL - 6的含量各组相比均无显著性差异 (P >0 0 5 )。结论　宫颈分泌物IL - 1β含量的升高与BV及早产有关 ,可能在BV引起的早产中起一定的作用     

阴道分泌物白细胞介素8与孕妇下生殖道感染关系的研究

目的　了解正常和下生殖道感染孕妇阴道分泌物白细胞介素 8(interleukin 8,IL 8)的水平变化特点与妊娠结局的关系。　方法　对下生殖道有霉菌、滴虫、沙眼衣原体、解脲支原体、人型支原体、细菌性阴道病感染或其中一种以上微生物感染的 2 5 5例观察组孕妇阴道分泌物进行IL 8定量检测及妊娠结局的随访观察 ,观察组中发生不良妊娠结局的孕妇取胚胎组织和胎盘组织进行病原体及病理检查 ,下生殖道无上述微生物感染的 12 0例正常孕妇作为对照组。　结果　 (1)观察A组在妊娠早、中、晚期IL 8浓度的平均值分别为 (2 2 2 .9± 193.6 )ng/L、(32 0 .0± 16 3.9)ng/L、(42 3.1± 115 .9)ng/L ,对照组IL 8浓度的平均值分别为 (12 5 .0± 4 5 .3)ng/L、(2 6 9.0± 5 9.5 )ng/L、(35 9.0± 10 7.1)ng/L ,两组比较差异有极显著性 (P <0 .0 1)。 (2 )IL 8>80 0ng/L的观察B组中用抗生素治疗的孕妇有 5 .2 6 %(2 / 38)发生异常妊娠结局 ,而未用抗生素治疗的孕妇有 78.13%(2 5 / 32 )发生异常妊娠结局 ,两组比较差异有极显著性 (P <0 .0 0 1)。 (3)妊娠结局异常孕妇的胚胎和胎盘组织中检出与宫颈分泌物相同的病原微生物 ,病理检测有绒毛膜炎性表现。　结论　IL 8水平的监测对早期预测下生殖道感染孕妇发生宫内感染和指导适     

乙型肝炎病毒感染孕妇IL-18对Th1/Th2细胞免疫平衡的作用与胎儿宫内感染相关性研究

目的：探讨乙型肝炎病毒（HBV）感染孕妇外周血白细胞介素-18（IL-18）水平对11hl／Th2细胞免疫的平衡作用与胎儿宫内感染的相关性。方法：选择2006年1月至2006年10月在本院分娩孕产妇101例，合并HBV感染、肝功能正常，单胎、足月妊娠。检测新生儿脐血HBV-DNA及HBsAg，诊断胎儿宫内感染2l例为研究组，无宫内感染80例为对照组。用双抗夹心酶联免疫吸附法（DAS-ELISA）检测孕妇外周血清IL-18、IFN-1（γ-干扰素）、IL4的水平。结果：（1）研究组孕妇外周血IL-18及IFN-1明显低于对照组，而IL4水平明显高于对照组，两组差异有统计学意义（P〈0．05，P〈0．0l，P〈0．05）；研究组IFN-γ／IL-4比值较对照组明显降低，差异有统计学意义（P〈0．01）。（2）两组孕妇外周血IL-18与IFN-γ均呈显著正相关、与IL-4水平均呈显著负相关、与IFN-γ／IL-4比值均呈显著正相关。结论：HBV宫内感染与孕妇Thl型特异性反应降低以及Th2型特异性反应增强有关，Thl／Th2免疫平衡失调可能是胎儿HBV宫内感染的机制之一；HBV宫内感染与孕妇外周血IL-18分泌下降有关，IL-18水平下调可能是HBV宫内感染的危险因素之一。     

细胞因子IL-6mRNA与IL-10mRNA在早产合并绒毛膜羊膜炎胎盘组织中的表达水平及意义

目的:研究白细胞介素-6 mRNA(IL-6 mRNA)和白细胞介素-10 mRNA(IL-10 mRNA)在早产合并绒毛膜羊膜炎孕妇胎盘组织中的表达水平,探讨IL-6及IL-10在妊娠中的作用,以及其在早产合并绒毛膜羊膜炎中的变化及意义。方法:取足月分娩及早产孕妇部分胎盘胎膜组织做病理检查,根据病检结果分为3组:早产感染组20例、早产非感染组20例和足月组20例。采用二步法RT-PCR法测定3组胎盘组织中IL-6 mRNA和IL-10 mRNA的表达。结果:早产感染组胎盘组织中IL-6 mRNA表达水平明显高于早产非感染组和足月组,差异有显著性(P<0.05);而IL-10 mRNA表达水平明显低于另外两组,差异有显著性(P<0.05)。结论:感染可引起细胞因子IL-6 mRNA、IL-10 mRNA在胎盘组织中的表达发生改变,推测IL-6可促进分娩发动,使妊娠提前终止,而IL-10在维持正常妊娠中可能起重要作用。     

孕妇产前进行免疫阻断与新生儿乙型肝炎基因疫苗免疫效果关系的研究

目的探讨采用乙型肝炎免疫球蛋白(HBIG)阻断孕妇乙型肝炎病毒(HBV)感染对新生儿乙型肝炎(简称乙肝)基因疫苗免疫效果的影响。方法对55例HBV标志物阳性孕妇于产前28周、32周和36周分别给予HBIG 200IU免疫阻断作为阻断组；31例HBV标志物阳性孕妇未给予HBIG免疫阻断作为未阻断组；同期选择HBV标志物阴性孕妇42例作为对照组。对三组新生儿分别给予乙肝基因疫苗的免疫接种，并分别于1个月、2个月、7个月和12个月龄采集外周血检测HBV标志物及丙氨酸转氨酶(ALT)。结果阻断组、未阻断组和对照组新生儿免疫保护率分别为87.3%(48/55)、77.4%(24/31)和97.6%(41/42)；未阻断组与对照组间比较具有统计学意义(P<0.01)；对“大三阳”孕妇的阻断效果最好，新生儿抗HBs阳转率从33.3%上升到71.4%。结论对HBV感染孕妇采用HBIG免疫阻断，可以降低宫内感染及母婴传播的发生率；分娩时孕妇HBV感染状态对新生儿抗HBs阳转率可能产生一定程度的影响。     

乙肝表面抗原阳性孕妇肌注乙肝免疫球蛋白预防母婴传播的临床研究

目的　探讨经母亲对胎儿行被动免疫在预防乙型肝炎病毒(HBV) 宫内感染中的作用。方法　对自孕20 周起多次肌注乙肝免疫球蛋白(HBIG) 的HBsAg( ) 孕妇34 例(A 组) 及未注射的14 例HBsAg( ) 孕妇(B 组) ,用固相放免法和套式PCR 检测母血HBsAg 、HBV DNA 及其新生儿血HBsAg 、抗HBS、HBV DNA。结果　A 组35例新生儿中32 例血清抗HBS( ) ,与B 组相比具有显著差异( P < 0-05) 。A 组新生儿血HBsAg 、HBV DNA 检出率均明显低于B 组。A 组孕妇用药后血HBsAg 滴度及HBVDNA 水平较用药前明显下降。结论　经母亲对胎儿行被动免疫可有效预防HBV 宫内感染。     

免疫阻断乙型肝炎病毒母婴传播多中心研究

目的 探讨孕产妇乙型肝炎表面抗原（HBsAg）阳性率及乙型肝炎病毒（HBV）母婴传播阻断的效果。方法 2008－2012年,通过多中心队列研究,对湖北省、山西省、广东省、新疆维吾尔自治区等地的孕产妇进行HBsAg筛查;对上述地区部分医院入院分娩的HBsAg阳性母亲及8～12个月龄婴儿进行随访观察,所有标本检测乙型肝炎血清标志物(HBsAg,HBsAb,HBeAg,HBeAb,HBcAb),部分标本检测HBV DNA。结果　筛查孕妇82214例,HBsAg阳性4924例,阳性率6.0%。随访HBsAg阳性母亲及8～12个月龄婴儿1371对,婴儿免疫阻断失败率3.1%（42/1371）,HBsAg及HBeAg双阳性母亲婴儿的免疫阻断失败率为8.2%。免疫阻断失败的婴儿其母亲均为HBeAg阳性且HBV DNA≥6 log10 copies/mL。HBeAg阳性母亲孕期注射乙型肝炎免疫球蛋白(hepatitis B immune globulin, HBIG)及未注射HBIG组,其婴儿免疫阻断失败率差异无统计学意义(8.8% vs. 8.1%, P=0.807)。结论　多中心调查显示目前孕产妇HBsAg阳性率6.0%,HBV母婴阻断失败率3.1%。HBsAg及HBeAg双阳性且HBV DNA≥6 log10 copies/mL 的孕妇应为母婴阻断的重点人群。孕妇孕期注射HBIG不能提高HBV母婴阻断效果。     

血清IL-6检测与胎膜早破相关性研究

目的探讨孕妇血清IL6与胎膜早破及绒毛膜羊膜炎的关系。方法2004年1～10月中山大学附属第三医院对46例胎膜早破孕妇和50例正常孕妇,采用ELISA法测定血清IL6质量浓度,分娩时胎盘胎膜送病理检查。结果胎膜早破孕妇血清IL6质量浓度明显高于对照组,并随破膜时间的延长其含量升高(P<0.05),而且有绒毛膜羊膜炎者血清IL6高于无绒毛膜羊膜炎者及对照组(P<0.01);38例自然临产的胎膜早破孕妇中,血清IL6≥30ng/L者,其取样距临产时间短于血清<30ng/L者(P<0.05)。结论血清IL6含量升高对早期诊断胎膜早破合并绒毛膜羊膜炎及预测分娩具有重要意义。     

子宫内膜异位症患者腹腔液中IL-6、IL-8、IL-10含量升高及其临床意义

目的：检测子宫内膜异位症（endometriosis,EMS）患者腹腔液中IL-6、IL-8、IL-10含量及其临床意义。方法：剖腹探查手术中采集EMS组及对照组的腹腔液,用酶联免疫吸附法（ELISA）测细胞因子水平。EMS组中,13份测IL-6,16份测IL-8,22份测IL-10,共51份,对照组检测57份。结果：EMS组和对照组IL-6的含量分别为155．88±114．92ng／L和28．74±25．88ng／L（P＜0．01）。IL-8的含量分别为630．97±404．72ng／L和63．05±62．3ng／L（P＜0．01）。IL-10也明显高于对照组［（38．88±38．75ng／L比10．45±4．33ng／L（P＜0．01）〕。结论：EMS患者腹腔液中IL-6、IL-8及IL-10均升高,提示EMS患者的巨噬细胞活性增强,细胞因子含量增加,可干扰患者免疫调节功能。     

IL-4和IL-12在宫颈癌的表达及临床意义

目的:探讨白细胞介素-4(IL-4)和白细胞介素-12(IL-12)在宫颈癌的表达及临床意义。方法:应用免疫组化PV6000通用二步法,检测IL-4和IL-12在20例正常宫颈上皮组织和72例宫颈癌组织蜡块中的表达,分析其与宫颈癌临床病理参数之间的关系。结果:IL-4在正常对照组和宫颈癌组的阳性表达率分别是20%和63.9%,两者差异有统计学意义(P<0.01);IL-12在两组的阳性表达率分别是55%和48.6%,两者差异无统计学意义(P>0.05)。IL-4在宫颈癌的表达与FIGO分期及有无淋巴结转移有关(P<0.05),IL-12表达与宫颈癌分化程度及淋巴结转移有关(P<0.05)。结论:IL-4高表达和IL-12低表达可能参与了宫颈肿瘤细胞的免疫逃逸机制,其程度高低提示肿瘤生长速度及浸润程度。     

Expression imbalance of IL-17/IL-35 in peripheral blood and placental tissue of pregnant women in preeclampsia

ObjectivesThe possible mechanism of preeclampsia is investigated in this study to facilitate the exploration of the future remediation of this disease by analysing the changes of IL-17 and IL-35 in peripheral blood and placental tissue of pregnant women with preeclampsia (PE).Materials and methodsThe study was conducted using 45 healthy pregnant women as the control group and 90 pregnant women in the preeclampsia group, including 45 cases with severe preeclampsia and 45 cases with mild preeclampsia. All of 135 pregnant women underwent caesarean delivery. IL-17 and IL-35 concentrations in the serum were measured by ELISA, and IL-17 and IL-35 expression in placental specimens was detected by immunohistochemistry.ResultsThere were no statistically significant differences in age among the three study groups. Serum IL-17 levels were significantly higher in PE patients than in healthy pregnant women (P < 0.01). The ratio of positive staining for IL-17 was markedly higher in mild PE tissues (84.44%; 38/45) and severe PE tissues (86.67%; 39/45) than in healthy pregnant tissues (35.56%; 16/45) (P < 0.01). The strong positive rates for IL-17 were markedly higher in mild PE tissues (48.89%; 22/45) and severe PE tissues (68.89%; 31/45) than in healthy pregnant tissues (13.33%; 6/45) (P < 0.01). No differences between mild PE tissues and severe PE tissues were noted in both positive case rates and strong positive rates. Consistent with this finding, the ratio of strong positive staining for IL-35 was higher in healthy pregnant tissues (66.67%; 30/45) than in mild PE tissues (33.11%; 14/45) and severe PE tissues (26.67%; 12/45) (P < 0.01).ConclusionsThe abnormal increase in serum and placental of IL-17 has an association with the formation and development of PE. IL-35 expression is significantly lower in severe PE placenta tissue and serum compared with normal pregnant women. These results suggested that IL-17/IL-35 imbalance may play a role in the pathophysiology of PE.     

母血羊水中IL—6和IL—8水平与绒毛膜羊膜炎关系的研究

目的 探讨白细胞介素－６（ＩＬ－６）和白细胞介素－８（ＩＬ－８）在监测胎早破中的作用。方法 采用酶联免疫吸附实验对４６例胎盘早破孕妇母血清,羊水中ＩＬ－６和ＩＬ－８水平进行监测,并以正常足月妊娠孕妇２０例做对照组。结果 胎膜早破孕妇母血清ＩＬ－６,ＩＬ－８和羊水中ＩＬ－６和ＩＬ－８水平均较正常足月妊娠组高。差异差异（Ｐ〈０．０１,Ｐ〈０．０５）;随着破膜时间延长母血ＩＬ－６、ＩＬ－８和羊水中ＩＬ－     

Alterations of IL-6, IL-6R and gp130 in early and late onset severe preeclampsia

Objective: Interleukin-6 (IL-6) and its receptor complex participate in a number of critical biological activities through several signaling pathways. Placental dysfunction and increased inflammation are believed to underlie the pathogenesis of severe preeclampsia (PE), which may involve IL-6-induced signaling. We investigate whether the changes in the expression of IL-6 and its cognate receptors, IL-6 receptor (IL-6R) and glycoprotein (gp)130, occur between early and late onset severe PE. Methods: A total of 18 healthy gravidas and 41 severe preeclamptic women were recruited, including 20 pregnancies with early onset and 21 pregnancies with late onset. The IL-6, soluble IL-6R (sIL-6R) and soluble gp130 (sgp130) levels in maternal and umbilical cord sera were tested by enzyme-linked immunosorbent assay; the immunoactivities of IL-6, IL-6R and gp130 in the placentas were determined by immunohistochemistry and immunoblotting. Results: We found that (1) maternal serum IL-6 and sIL-6R levels were increased, whereas sgp130 was decreased in women with early onset severe PE compared with those with late onset severe PE and normal controls. In contrast to normal controls, IL-6 and sIL-6R expression was up-regulated in late onset severe PE, and similar alterations of IL-6 and its soluble receptors were detected in early and late onset severe PE umbilical sera and (2) compared with late onset severe PE, reduced IL-6 and IL-6R but not gp130 expression was observed in early onset severe PE placentas. Conclusion: These results suggest a probable contributing role of alterations in IL-6 and its corresponding receptors to the pathophysiology of early and late onset severe PE.     

输精管结扎家兔外周血淋巴细胞丝裂原反应性、IL－2及巨噬细胞IL－1活性的动态研究

体重２．５～３．０ｋｇ的日本大耳白雄兔２８只，分为输精管结扎组（ＶＧ，１８只）和假手术对照组（ＳＯＧ，１０只）。ＶＧ行双侧输精管结扎手术，ＳＯＧ只行双侧输精管暴露手术，术后第４、８、１２、１８、２２月检测外周血淋巴细胞对丝裂原反应性、ＩＬ—２和巨噬细胞ＩＬ－１活性。结果表明：１．ＣｏｎＡ和ＰＨＡ诱导的淋巴细胞丝裂原反应性，术后第８月ＶＧ的３Ｈ—ＴｄＲ掺入量均明显低于明Ｇ（Ｐ＜０．０１），而术后第４、１２、１８、２２月的测定结果与ＳＯＧ比较均无差异（Ｐ＞０．０５）。２．ＩＬ－２活性也以术后第８月为最低，与ＳＯＧ比较差异显著（Ｐ＜０．０１），１２月后恢复正常。３．ＣｏｎＡ诱导的淋巴细胞丝裂原反应性和ＩＬ－２活性间呈有意义的正相关关系（ｒ＝０．６６１，Ｐ＜０．００１）。４．ＩＬ－１活性，ＶＧ第８月显著地高于ＳＯＧ第８月（Ｐ＜０．０５），其它各月检测结果两组比较无明显差异。     

A disproportionate increase in IL-1beta over IL-1ra in the cervicovaginal secretions of pregnant women with altered vaginal microflora correlates with preterm birth

OBJECTIVE: This purpose of this study was to investigate the association between vaginal microflora, concentrations of interleukin-1beta (IL-1beta), and its natural receptor antagonist (IL-1ra) in the cervicovaginal discharge, and spontaneous preterm birth.Study design Vaginal samples collected at 18 to 22 weeks' gestation from 207 women were analyzed to study qualitative and quantitative microbiologic aspects of vaginal microflora and IL-1beta and IL-1ra concentrations. RESULTS: Among women colonized with anaerobic Gram-negative rods and/or Gardnerella vaginalis, an elevated IL-1beta concentration, or a diminished IL-1ra:IL-1beta ratio were associated with preterm delivery. A cut-off IL-1ra:IL-1beta ratio of <8632:1 optimally discriminated the subjects with subsequent spontaneous preterm deliveries from subjects who delivered at term, with a sensitivity of 78%, specificity of 51%, positive predictive value of 21%, and negative predictive value of 95%. CONCLUSION: A disproportionate increase in IL-1beta over IL-1ra in response to vaginal colonization with anaerobic Gram-negative rods and/or G. vaginalis at 18 to 22 weeks' gestation is associated with spontaneous preterm delivery.     

Association between polymorphisms in IL-27 gene and pre-eclampsia

Pre-eclampsia is a complex pregnancy-specific hypertensive syndrome, and it is a leading cause of maternal and neonatal death worldwide. We aimed to evaluate the associations between polymorphisms of IL-27 gene and pre-eclampsia susceptibility in Han Chinese women.Methods663 pregnant women were enrolled in a case-control study (212 cases and 451 normal pregnancies). The rs153109 and rs17855750 variants were discriminated using Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR–RFLP) methods.ResultsA significantly reduced risk of pre-eclampsia was observed in the rs153109 GG genotype compared with the AA or AA/AG genotypes (GG versus AA: OR = 0.51, 95%CI = 0.30–0.86; GG versus AA/AG: OR = 0.60, 95%CI = 0.37–0.98). Significantly reduced pre-eclampsia susceptibility was also associated with the AG/GG genotypes of rs153109 (OR = 0.68, 95%CI = 0.49–0.94) in dominant model. After stratification analysis, the different distribution of AG/GG genotypes was particular significant in the severe pre-eclampsia subgroup (OR = 0.65, 95%CI = 0.45–0.92) and the early-onset severe pre-eclampsia subgroup (OR = 0.51, 95%CI = 0.30–0.87). Additionally, significantly increased mild pre-eclampsia risk was observed associated with rs17855750 GT/GG and GT genotypes when compared with TT and TT/GG genotypes (GT/GG versus TT: OR = 2.27, 95%CI = 1.12–4.55; GT versus TT/GG: OR = 2.56, 95%CI = 1.28–5.26).ConclusionIt is biologically plausible that SNPs in IL-27 may have effect on individual susceptibility to pre-eclampsia. The results suggest the presence IL-27 rs153109, rs17855750 variants may be able to be used as markers for the genetic susceptibility to pre-eclampsia.     

子宫内膜异位症患者子宫内膜IL-18表达的研究

目的 :研究子宫内膜异位症患者子宫在位内膜和异位内膜IL 18的表达 ,探讨IL 18在内异症发病机制中的意义。方法 :用免疫组化和半定量逆转录聚合酶链反应法检测对照组子宫内膜、子宫腺肌症患者子宫内膜和内异症患者的子宫在位内膜与异位内膜IL 18蛋白的表达位置及其mRNA表达水平。结果 :各组标本IL 18蛋白和mRNA的表达均阳性 ,内异症患者子宫在位内膜与异位内膜的IL 18mRNA表达水平分别为 1.0 5±0 .4 0、0 .77± 0 .39,均低于对照组子宫内膜 (1.6 5± 0 .6 4) ,差异有显著性 (P <0 .0 5 ) ;子宫腺肌症组IL 18mRNA表达水平为 1.6 3± 0 .6 0 ,与对照组子宫内膜IL 18mRNA表达水平差异无显著性。结论 :IL 18可能参与了子宫内膜异位症的发病 ,IL 18mRNA在内异症患者在位和异位内膜的低水平表达 ,可能是影响内异症形成和发展的重要因素之一。     

经阴道注入重组白细胞介素17对正常妊娠CBA/J孕鼠的影响

目的：观察经阴道注入重组白细胞介素17（r IL-17）对正常妊娠CBA/J孕鼠（CBA/J雌鼠×BALB/c雄鼠）妊娠结局的影响,探讨其在诱导母胎免疫排斥中的作用。方法：将6-8周龄清洁级CBA/J（雌）和BALB/c（雄）近交系小鼠分为3组。对照组：正常妊娠模型CBA/J孕鼠未作任何干扰。磷酸盐缓冲液（PBS）组：正常妊娠模型CBA/J孕鼠,于受孕第1天麻醉状态下经阴道注射50μL PBS。r IL-17组：正常妊娠模型CBA/J孕鼠,于受孕第1天麻醉状态下经阴道注射50μL r IL-17。受孕后第14天观察胚胎丢失率。通过实时荧光定量聚合酶链反应（q RT-PCR）方法、蛋白质印迹（Western blotting）方法检测鼠蜕膜转化生长因子β（TGF-β）和IL-10的表达。结果：给予正常妊娠模型孕鼠外源性r IL-17,能引起其胚胎丢失率明显增加,且蜕膜TGF-β和IL-10 m RNA及蛋白的表达均明显降低（P〈0.05）。而对照组与PBS组相比,胚胎丢失率及TGF-β和IL-10 m RNA及蛋白的表达差异均无统计学意义（均P〉0.05）。结论：经阴道注入r IL-17能够引起正常妊娠模型CBA/J孕鼠胚胎丢失率增加,并引起母胎界面免疫耐受细胞因子分泌异常。