角膜地形图仪与角膜曲率计测量结果的比较

目的　探讨角膜曲率计和角膜地形图检查系统测量角膜屈光力的差异。方法　随机选取我院屈光手术门诊LASIK术前 3 65例 ( 681眼 ) ,同时用两种方法测量角膜屈光力 ,比较二者间的差异。结果　两种方法测量的最大角膜屈光力 :角膜曲率计为 ( 4 3 774± 1 5 3 5 )D和角膜地形图仪为 ( 4 3 5 17± 1 5 46)D ,最小角膜屈光力分别为 ( 4 2 75 6± 1 43 0 )D和 ( 4 2 5 13±1 44 0 )D ;平均角膜屈光力分别为 ( 4 3 2 5 6± 1 45 9)D和 ( 4 2 769± 1 42 7)D。以上 3组数字两两比较 ,差异均有非常显著意义(P <0 0 0 1)。结论　角膜地形图仪所测角膜屈光力较角膜曲率计所测结果准确 ,LASIK术前应参考角膜地形图仪所测角膜屈光力。     

自动角膜曲率计与角膜地形图仪测量儿童角膜屈光力的比较研究

目的 比较KR-8100自动角膜曲率计与TMs-4角膜地形图仪测量屈光不正儿童角膜屈光力及散光轴向的差异,并对两种测量方法进行一致性评价.方法 采用KR-8100自动角膜曲率计及TMS-4角膜地形图仪对53例(106只眼)屈光不正儿童进行测量,应用配对t检验对两种方法测量的陡峭 子午线角膜屈光力(Ks)、平坦子午线角膜屈光力(Kf)、角膜散光Ks-Kf大小及轴向进行比较,并应用Bland-Altmann分析对两种测量方法进行一致性评价.结果 角膜地形图仪测量的Ks、Kf、Ks-Kf值均高于角膜曲率计的测量值(P<0.01);角膜地形图仪与角膜曲率计的散光轴向测量值差异有统计学意义(P<0.01).两种仪器测量Ks的差值的均数为1.72D,测量Kf的差值的均数为1.37D,这种测量差别临床上不能接受,说明两种仪器测量角膜屈光力的一致性较差.结论 KR-8100自动角膜曲率计与TMS-4角膜地形图两种测量方法在临床上不能替代使用.     

角膜后表面屈光力理论计算值与Orbscan测量值的比较

目的 比较角膜后表面屈光力理论计算值与角膜地形图(Orbscan)测量值之间的差异,评价Orbscan在角膜屈光手术领域的应用价值。 方法 应用球面折射公式计算64例64眼角膜后表面屈光力;Orbscan测量受试者角膜后表面屈光力。结果 角膜后表面屈光力Orbscan平均测量值为-6.59 D±0.22 D,平均理论计算值为-5.04 D±0.14 D,Orbscan平均测量值与平均理论计算值差异为-1.55 D±0.19 D(P<0.01)。 结论 角膜后表面屈光力Orbscan测量值大于理论计算值-1.55 D。Orbscan测量角膜后表面屈光力的准确性尚需进一步探讨。     

基于Pentacam对角膜屈光指数的评价

目的：应用Pentacam测量角膜中央3 mm前表面曲率（Ra ）、后表面曲率（Rp）及中央角膜厚度（CCT）， 根据测量结果，探讨角膜屈光力及角膜屈光指数的计算方法。方法：系列病例研究。选取2017年2─10月在湘潭市中心医院和温州医科大学附属眼视光医院接受角膜屈光手术或白内障手术术前检查的患者419例（419眼）。术前采用Pentacam对眼前节进行检查，采集角膜中央3 mm Ra、RP及CCT 的相关数据，计算Ra与Rp比值（AP ratio）、角膜前表面屈光力（Ka ）以及后表面屈光力（Kp），按照厚 透镜高斯光学公式计算角膜总屈光力（KGOF）及角膜屈光指数（Ncal）。采用配对样本t检验比较各种K 值之间的差异。结果：Pentacam测量所得Ra、Rp、Rsimk、CCT、SimK分别为（7.73±0.27）mm、（6.34± 0.24）mm、（7.73±0.27）mm、（537±33）μm、（43.65±1.52）D。AP ratio为1.220±0.026，角膜屈光指数 Ncal为1.328±0.001。结论：利用Pentacam测量角膜参数计算所得角膜屈光指数可以提高角膜总屈光力及角膜后表面屈光力预算的准确性。     

角膜塑形术前后角膜地形图与角膜曲率计一致性研究

目的比较角膜塑形术前后EyeSys角膜地形图系统与角膜曲率计测量角膜屈光力与散光轴向的差异。方法对进行角膜塑形术治疗的321例患者在术前及术后1d、术后1个月分别进行角膜地形图及角膜曲率计的检查,对两种方法所测得的水平子午线角膜屈光力(HK)、垂直子午线屈光力(VK)、角膜散光及轴向进行比较。结果角膜塑形术前、术后1d、术后1个月,角膜地形图仪测量的HK值分别为(43.25±1.16)D、(42.88±1.18)D、(42.79±1.87)D,较角膜曲率计测量的HK值分别高0.20D、0.39D、0.40D,差异均有显著统计学意义(均为P<0.01)。角膜地形图仪测量的VK值分别为(44.39±1.33)D、(43.96±1.33)D、(43.02±1.64)D,较角膜曲率计测量的VK值高0.13D、0.32D、0.73D,差异均有显著统计学意义(均为P<0.01)。角膜地形图仪测得角膜散光值均较角膜曲率计小,术前、术后1d、术后1个月分别小0.06D、0.10D、0.40D。术后两种方法测量HK、VK、角膜散光及散光轴向之间的差异逐渐增大(P<0.01),并且两种方法的轴向差异朝着逐渐变大的方向分布。但两种方法对顺规及非顺规散光的检出率差异均无统计学意义(均为P>0.05)。结论 EyeSys角膜地形图系统可以更全面及准确地了解角膜塑形术过程中的角膜情况,故临床上应以角膜地形图的结果作为主要依据,角膜曲率计的结果仅可以作为参考。     

后曲率实测法计算准分子激光角膜原位磨镶术后角膜屈光力的准确性

目的 探讨后曲率实测法计算准分子激光原位角膜磨镶术(LASIK)后角膜屈光力的准确性.方法 多种测量角膜屈光力方法的比较性研究.回顾性分析按后曲率实测法计算人工晶状体度数的LASIK术后人工晶状体植入眼8例(11只眼,10只为超声乳化白内障吸除及人工晶状体植入术,1只为人工晶状体置换术),计算术后稳定屈光状态与目标屈光度的差异,并据此推导实际角膜屈光力.分析其他角膜曲率法(自动曲率计、角膜地形图、球镜当量法、前曲率法、Pentacam提供的EKR曲率)计算人工晶状体度数可能造成的届光偏差.对LASIK术后6个月随访眼23例行详细屈光检查,根据术前角膜屈光力及手术前后眼屈光度改变推导术后理论角膜屈光力.分析后曲率实测法计算所得角膜屈光力与理论角膜屈光力的差异,并与其他角膜曲率法作比较.结果 采用后曲率实测法计算的人工晶状体植入眼术后平均裸眼视力0.8±0.2,与目标屈光度绝对偏差平均为(0.36±0.36)D(-0.63～+0.85 D),绝对偏差≤0.25 D、≤0.50 D、≤1.00 D的眼比例数分别为55%、73%和91%.其屈光偏差显著低于自动曲率计[(2.50±1.08)D]、角膜地形图[(1.90±0.88)D]、球镜当量法[(2.09±1.62)D](P<0.01)及前曲率法[(1.45±1.10)D](P<0.05)的预期结果;与EKR曲率法比较差异无统计学意义,但其偏差范围(-1.13～0.85 D)小于后者(-1.10～1.80 D).23例单纯LASIK术后眼的角膜屈光力测算同样显示后曲率实测法计算所得角膜屈光力与理论角膜屈光力偏离程度最小,绝对偏差为(0.67±0.45)D.结论 后曲率实测法计算LASIK术后角膜屈光力,可行性准确性俱佳.     

Pentacam Topolyzer与角膜曲率计测量角膜屈光力的比较

目的 比较Scheimpflug原理摄像系统Pentacam与Placido盘成像Topolyzer角膜地形图及Topcon自动式角膜曲率计测量角膜屈光力的差异,分析三者的一致性,为临床使用提供依据.方法 随机选取拟行角膜屈光手术角膜形态正常的患者39例(39只眼).术前分别采用Pentacam、Topolyzer及Topcon自动式角膜曲率计测量角膜屈光力,并对测量结果进行统计分析.结果 Topcon自动式角膜曲率计测量值为(43.35±1.36)D,Topolyzer测量值为(43.41±1.35)D;Pentacam测量所得simK、true net power(TNK)、4.0mm Holladay equivalent K-reading(EKR)、4.5mm EKR分别为(43.40±1.34)D,(42.14±1.25)D,(43.44±1.31)D,(43.50±1.32)D.Pentacam测量所得simK、4.0mm EKR与Topolyzer测量所得simK差异无统计学意义(P＞0.05);其余各组K值之间比较差异均有统计学意义(P＜0.01).三种测量方法所得K值两两之间均存在高度正相关(r＞0.90,P＜0.01).TNK比其余各种K值均明显小(-1.21～-1.36)D,一致性较差;其余各组K值之间一致性较好,95％一致性界限较窄.结论 对于角膜形态正常的患者,Pentacam测量所得simK及4.0 mm EKR与Topcon自动式角膜曲率计测量所得Km及Topolyzer测量所得simK具有良好的一致性,不过在临床中互相替代使用仍需要慎重.true net power比其余各种K值均明显小,不能用于人工晶状体屈光度计算.     

巩膜宽环扎术前后角膜光学形态变化

目的观察巩膜宽环扎手术前后角膜形态学变化,进一步探讨巩膜宽环扎术对角膜光学形态的影响。方法用博士伦Obscan-Ⅱ角膜地形图仪测量孔源性视网膜脱离15例15眼术前、术后1周的角膜前表面曲率、角膜厚度及角膜后表面曲率。结果术后1周,在直径3 mm和5 mm区,角膜总屈光力、角膜前表面屈光力、角膜后表面屈光力及中央角膜厚度与术前相比,差异无显著性,在角膜前表面直径5 mm区、角膜后表面直径3 mm和5 mm区散光增加,角膜形状系数(shape factor,SF)减小。结论巩膜宽环扎术后早期,角膜5 mm区内屈光力改变不明显,但可导致角膜前后表面散光增加;角膜中央向周边逐渐变平坦的幅度变小。     

准分子激光原位角膜磨镶术后OrbscanⅡ估算术前角膜屈光力

目的 比较OrbscanⅡ测量准分子激光原位角膜磨镶术(LASIK)前后周边角膜后表面曲率半径改变,初步确立新的角膜前后表面曲率半径比率R,利用术后周边后表面角膜曲率半径和比率R估算术前角膜总屈光力。方法 回顾分析OrbscanⅡ测量151只眼术前及术后3个月角膜7～10 mm区后表面曲率半径值的变化,计算角膜0～～7 mm区前表面曲率半径与角膜7～10 mm区后表面曲率半径比率R,利用比率R推算30只眼术前角膜总屈光力,并验证计算性角膜总屈光值与测量性角膜总屈光值的一致性。LASIK手术前后角膜7～10 mm后表面曲率半径变化和计算性角膜总屈光力与术前OrbscanⅡ测得角膜总屈光力比较采用配对t检验。结果 LASIK术前后角膜7～10 mm区后表面曲率半径差值为（-0.005±0.154）mm(t=0.417,P=0.677),术前角膜0～7 mm区前表面曲率半径与角膜7 ～ 10 mm区后表面曲率半径比率R为1.167±0.030,利用比率R推算的角膜总屈光力均值为(43.49±1.79)D,OrbscanⅡ测得实际角膜总屈光力均值为(43.77±1.53)D,两者差值均值为（-0.28±1.00）D(t=-1.523,P=0.139)。结论 LASIK手术前后周边区域角膜后表面曲率半径改变无实际临床意义,计算所得新角膜前后表面曲率半径比率R可准确的推算LASIK术者术前角膜总屈光力,为LASIK术后丢失术前资料患者双K法计算人工晶状体度数提供重要条件。     

准分子激光原位角膜磨镶术后角膜后表面屈光力变化

目的 探讨准分子激光原位角膜磨镶术(LASIK)后角膜后表面屈光力变化对角膜总屈光力的影响及其在术后屈光回退中所起的作用;分析角膜后表面屈光力变化的相关因素。 方法 对47例89眼施行LASIK,其中49眼高度近视,40眼中低度近视。术前与术后1个月,应用裂隙扫描角膜地形网检测角膜前后表面屈光力及隆起度。分析角膜后表面屈光力及角膜后表面隆起度变化的相关因素。 结果LASIK术后.角膜后表面屈光力与角膜后表面隆起度的改变均具有统计学意义(-6.64±0.23)D/(-7.19±O.28)D,P<0.05;(24.57±10.74)μm/(56.98±15.60)μm,P相似文献   

美容性角膜覆盖术疗效观察

目的:评价美容性角膜覆盖术治疗角膜(角巩膜)葡萄肿的临床效果。方法:对22例角膜(角巩膜)葡萄肿患者进行了美容性角膜覆盖术治疗。术后对所有病例的临床效果进行了随访观察。结果:22例均治愈,残留部分视力的2例的视力术后有所改善(2例2眼从术前手动/眼前到术后指数/眼前)。22例无1例发生排斥,角膜覆盖片的颜色除第1例因缺乏经验术眼的颜色明显深于对侧正常或正常人群眼的颜色外,其它无1例褪色。结论:应用角膜覆盖术治疗角膜(角巩膜)葡萄肿22例(22眼)临床证明疗效确实、安全可靠。     

Anterior amorphous corneal opacity and corneal thinning

PurposeTo present the clinical features of four cases with bilateral anterior amorphous corneal opacity.MethodsA retrospective study in four patients with bilateral anterior amorphous corneal opacity was conducted. Examinations included visual acuity, keratometry, slit-lamp biomicroscopy, confocal microscopy, anterior segment optical coherence topography, and histology.ResultsThree female and one male patients (mean age, 52.3 ± 8.9 years) showed bilaterally oval, amorphous sheetlike corneal opacities with central depression and thinning. Superior limbal opacities were observed in two of these patients. The best-corrected visual acuity ranged from 20/50 to 20/400, and the mean of the keratometry was 39.81 ± 3.97 D (diopters). They had mild dry eyes. The anterior segment optical coherence topography demonstrated hyporeflective abnormalities in the anterior depressed stroma in these four patients. Confocal microscopy revealed large round cells at the epithelial layer in one patient, and amorphous opacities with some strand-shaped opacities in the anterior stroma in all four patients. The mean of the corneal endothelial cells density in the eight eyes was 1521 ± 402 cells/mm². Central corneal stromalysis occurred in three patients, and descemetocele developed in two eyes. One patient received penetrating keratoplasty and two underwent lamellar keratoplasty. The histology of the corneal specimen revealed edematous basal epithelial cells, focal collagen disorganization in the thin stroma, and wartlike excrescences in a thickened Descemet's membrane.ConclusionAnterior amorphous corneal opacity is a rare keratopathy and may be one kind of rare corneal degeneration or dystrophy. Corneal stromalysis may occur in hyporefrective amorphous opacities and progress to descemetocele.     

Influence of corneal astigmatism, corneal curvature and meridional differences on corneal hysteresis and corneal resistance factor

Background: This study investigated the influence of corneal astigmatism, corneal curvature and meridional differences on corneal hysteresis (CH) and the corneal resistance factor (CRF) in a group of normal Chinese persons. Methods: Ninety‐five participants were recruited and data from the eye with higher corneal astigmatism were analysed. The anterior corneal curvature was measured by corneal topography. The Goldmann‐correlated intraocular pressure (IOPg), corneal‐compensated intraocular pressure (IOPcc), CH and CRF at different meridians (default horizontal position, 10°, 20° and 30° along the superotemporal and inferonasal meridians) were obtained from an ocular response analyser. The corneal powers at these specific meridians also were calculated. Results: At the default position, the IOPg and CRF had weak correlations with corneal astigmatism, while the IOPcc and CH were not significantly correlated with corneal astigmatism. Both the IOPg and IOPcc were measured significantly higher at the default position. The CH and CRF were lower at the default position but the difference in the CRF from obliquity could not reach statistical significance. The CH was not significantly correlated with the corneal power at all meridians. The CRF correlated with the corneal power only at 30° superotemporal. Conclusion: Corneal astigmatism and head tilt did not have much effect on the measurement of CH and the CRF, both of which were lowest along the horizontal meridian. Clinically, the difference was small. The influence of corneal power on CH and the CRF was minimal.     

角膜生物力学与光密度相关性研究

目的:探究角膜生物力学与角膜光密度的相关性。方法:前瞻性研究。选取2019-03/06在云南省第二人民医院拟行角膜屈光手术术前检查的患者为研究对象。采用Pentacam HR眼前节分析系统进行角膜光密度测量,以角膜顶点为中心,分为0~2mm、> 2~6mm、> 6~10mm直径范围3个区域,以角膜厚度分为前、中、后3层。选取Pentacam HR中角膜最薄点厚度值纳入研究。采用Corvis ST角膜生物力学分析仪测量,相关参数包括第一次压平的长度(AP1L)和速率(AP1V)、第2次压平的长度(AP2L)和速率(AP2V)、最大凹陷时顶点距离(PD)、曲率半径(HCR)和形变幅度(DA)。运用Pentacam&Corvis ST生物力学联合诊断平台软件综合分析检查结果,得出综合角膜生物力学参数(CBI)以及其它独立参数包括硬度参数(SP)、综合半径(IR)、Ambrosio相关厚度-水平方向(ARTh)、形变幅度比(DAR)。各区域光密度间差异采用方差分析,角膜生物力学各项参数与各区域光密度的相关性采用Pearson或Spearman分析。结果:不同直径范围、不同层面间光密度有差异(F=35.101,P<0.01;F=1002.897,P<0.01),CBI与独立生物力学参数中AP2L、AP2V、PD、DA、SP、IR、ARTh、DAR具有相关性(rs=-0.502,P<0.01;rs=-0.457,P=0.001;rs=0.428,P=0.002;rs=0.539,P<0.01;rs=-0.687,P<0.01;rs=0.716,P<0.01;rs=-0.728,P<0.01;rs=0.750,P<0.01)。CBI与角膜0~2mm范围内光密度呈正相关(r=0.343,P=0.015)。0~2mm范围内光密度与独立生物力学参数中AP2L、IR、ARTh、DAR有相关性(rs=-0.298,P=0.035;rs=0.368,P=0.009;rs=-0.419,P=0.002;rs=0.493,P<0.01)。结论:角膜中央区域光密度与角膜生物力学具有显著的关联,临床中可以通过光密度和生物力学对角膜健康状况进行综合评价。     

角膜基质透镜在角膜溃疡或穿孔修复中的应用

目的：研究由全飞秒激光SMILE手术所得的角膜基质透镜作为角膜移植材料治疗角膜溃疡的临床疗效。

方法：回顾性病例研究。收集本院2017-01/06角膜溃疡患者6例6眼,其中细菌性、真菌性、深层异物伴感染各1例1眼,角膜穿孔3例3眼。采用由全飞秒激光SMILE手术所得的角膜基质透镜作为角膜移植的材料进行修复手术,确保植片与角膜层间无空气间隙。术后随访1～6(平均3.71±1.56)mo,观察手术前后视力、角膜移植物存活情况及术后并发症发生情况等。

结果：所有患者均在控制感染下顺利完成手术,无术中并发症。术后所有角膜植片透明。末次随访时,患者最佳矫正视力(best corrected visual acuity,BCVA)较术前明显改善(0.48±0.12 vs 1.50±0.08),差异具有统计学意义(P<0.01)。

结论：来源于全飞秒激光屈光手术的角膜基质透镜用于角膜溃疡修复是安全有效的,但植片的远期疗效尚需进一步观察。     

Alcohol delamination of the corneal epithelium for recurrent corneal erosion syndrome

AIM: To evaluate the outcomes of alcohol delamination (ALD) of the corneal epithelium for the treatment of recurrent corneal erosion syndrome (RCES) and to implement a standardized treatment protocol for this condition utilizing evidence based practice and the findings of an internal audit. METHODS: A retrospective analysis of 42 eyes of 40 patients diagnosed with RCES who were treated with ALD between January 2006 and March 2016 was conducted. Patients had 20% alcohol applied to the cornea with the use of a well for 40s. Patients were reviewed one week later in the Outpatient Department. Outcome criteria were established based on standards from other studies in the medical literature. These included, a treatment success rate of at least 72% (defined as complete resolution of symptoms one month after treatment), a postoperative complication a rate of <5% (mainly infective keratitis, and subepithelial haze), and the absence of any detrimental effect on visual acuity in ≥95% of patients. RESULTS: The mean age at the time of ALD was 41.17±13.44y. Patients were followed for an average of 12.8±15.65mo. The majority were female (52.5%, n=21) and the majority of eyes treated with ALD were left eyes (62.9%, n=26). Trauma was the primary aetiology in our study population. Treatment was successful in 73.8% (n=31) of eyes and in 75% (n=30) of patients. Recurrence occurred in 26.2% of eyes at a mean of 10.41±12.63mo post treatment. CONCLUSION: ALD is an efficacious and cost-effective primary surgical intervention for RCES.     

Orbscan-II角膜地形图仪与超声角膜测厚仪角膜测厚结果的对比分析

目的:探讨Orbscan-II角膜地形图仪与角膜测厚仪对角膜测厚的结果差异的统计学及临床意义。方法:选115例204眼(右104眼,左100眼)分别行Orb-scan-II及超声测厚法测量角膜厚度,经统计学检验后进行分析。结果:204眼Orbscan-II所得结果为527.20±37.83,超声测厚法所得结果为525.25±34.83,行配对t检验,t=2.407,P=0.017,认为两种测量方法结果的差异有统计学意义。进一步将角膜厚度分4组进行配对t检验,角膜厚度<500μm时,Orbscan-II测厚(478.36±14.47)与超声测厚(478.3±20.15)差异无统计学意义,角膜厚度≥500,<550μm时,Orbscan-II测厚(527.88±17.32)与超声测厚(525.32±12.92)差异有统计学意义(P=0.027),角膜厚度≥550,<600μm时,Orbscan-II测厚(571.24±14.89)与超声测厚(568.04±13.82)差异有统计学意义(P=0.022),角膜厚度≥600μm时,Orbscan-II测厚(615.33±8.50)与超声测厚(615.33±14.29)差异无统计学意义。结论:传统的超声测厚与Orbscan-II对角膜测厚结果有一定差异,并具统计学意义,但在临床意义上二者仍有较好的相符性。     

配戴软性角膜接触镜对角膜中央厚度影响的临床研究

目的:探讨配戴软性角膜接触镜对中央角膜厚度的影响。方法:应用超声角膜测厚仪检测中央角膜厚度,在我院近视患者中随机抽取配戴软性角膜接触镜者及不戴镜者各100例200眼,做统计分析。再在近视患者中按戴镜时间<1a,1～3a,3～5a,5～7a,≥7a分组,每组随机抽取60例120眼,做统计分析。结果:配戴软性角膜接触镜者与不戴镜者的角膜厚度比较,差异有统计学意义(P<0.05)。戴镜时间<1a,1～3a,3～5a,5～7a者的角膜厚度比较,差异有统计学意义(P<0.05)。结论:配戴软性角膜接触镜者平均中央角膜厚度薄于不戴角膜接触镜者,并且配戴软性角膜接触镜的时间越长中央角膜厚度越薄。     

角膜内皮炎的角膜内皮细胞形态改变

目的探讨角膜内皮炎对患者中央角膜内皮细胞形态的影响。方法用EM-1000型接触式角膜内皮镜对10例单眼角膜内皮炎愈后4-12周的患者的双眼分别摄取中央角膜内皮像并对其图像进行电脑分析。患眼作为实验组。健眼作为对照组。观察其角膜内皮细胞密度。六角形细胞的百分比及异形性的变化。用计量资料配对设计的2样本均数的t检验进行统计分析（双侧检验，P〈0．01为统计学有差异）。结果在10例临床诊断角膜内皮炎愈后的患者中，中央角膜内皮细胞密度实验组平均为（1981±181）／mm^2。对照组平均为（2284±315）／mm^2。六角形细胞比例实验组平均为34％，对照组平均为43％．变异系数实验组平均为53％，对照组平均为45％．以上各项观察指标实验组与对照组之间在统计学上都存在显著性差异（P〈0．01）。结论角膜内皮炎对角膜内皮细胞造成损伤，导致严重的形态改变，在临床工作中应给予充分重视。[眼科新进展2007；27（2）：138-139]     

Construction of a full-thickness human corneal substitute from anterior acellular porcine corneal matrix and human corneal cells

AIM: To construct functional human full-thickness corneal replacements. METHODS: Acellular porcine corneal matrix (APCM) was developed from porcine cornea by decellulariztion. The biomechanical properties of anterior-APCM (AAPCM) and posterior-APCM (PAPCM) were checked using uniaxial tensile testing. Human corneal cells were obtained by cell culture. Suspending ring was designed by deformation of an acupuncture needle. MTT cytotoxicity assay was used to check the cytotoxicity of suspending ring soaking solutions. A new three-dimensional organ culture system was established by combination of suspending ring, 48-well plate and medium together. A human full-thickness corneal substitute was constructed from human corneal cells with AAPCM in an organ coculture system. Biochemical marker expression of the construct was measured by immunofluorescent staining and morphological structures were observed using scanning electron microscopy. Pump function and biophysical properties were examined by penetrating keratoplasty and follow-up clinical observations. RESULTS: There were no cells in the AAPCM or PAPCM, whereas collagen fibers, Bowman’s membrane, and Descemet’s membrane were retained. The biomechanical property of AAPCM was better than PAPCM. Human corneal cells grew better on the AAPCM than on the PAPCM. There was no cytotoxicity for the suspending ring soaking solutions. For the constructed full-depth human corneal replacements keratocytes scattered uniformly throughout the AAPCM and expressed vimentin. The epithelial layer was located on the surface of Bowman’s membrane and composed of three or four layers of epithelial cells expressing cytokeratin 3. One layer of endothelial cells covered the stromal surface of AAPCM, expressed Na+/K+ATPase and formed the endothelial layer. The construct was similar to normal human corneas, with many microvilli on the epithelial cell surface, stromal cells with a long shuttle shape, and zonula occludens on the interface of endothelial cells. The construct withstood surgical procedures during penetrating keratoplasty. The corneal transparency increased gradually and was almost completely restored 7d after surgery. CONCLUSION: AAPCM is an ideal scaffold for constructing full-thickness corneal replacement, and functional human full-thickness corneal replacements are successfully constructed using AAPCM and human corneal cells.