纤维粘连蛋白在乳腺癌中的表达及与淋巴结转移的关系

目的 研究纤维粘连蛋白（ＦＮ）在乳腺浸润性癌中的表达及其与同侧腋窝淋巴结转移的关系。方法 应用免疫组化（ＳＰ）法检测５０例浸润性癌底膜及间质ＦＮ的表达。结果 癌巢周围基底膜ＦＮ的表达与癌细胞分化程度及淋巴结转移有关,而间质ＦＮ的表达与以上二者均无关,结论癌巢周围基底膜ＦＮ的表达对乳腺癌预后的评估有意义。     

纤维连接蛋白、层黏连蛋白、细胞外基质金属蛋白酶诱导因子在食管癌的表达

目的 研究纤维连接蛋白(FN)、层黏连蛋白(LN)、细胞外基质金属蛋白酶诱导因子(EMMPRIN)在食管癌的表达,探讨其临床意义.方法 采用免疫组织化学法对87例食管癌组织、30例正常食管组织、20例不典型增生食管组织进行FN、LN、EMMPRIN检测.结果 正常食管组织基底膜FN、LN染色呈连续线状;低分化癌巢周围基底膜FN、LN染色表现为碎片状;FN、LN表达与食管癌病理分级、淋巴结转移有关;EMMPRIN的弱阳性表达与食管癌淋巴结转移有关,强阳性表达与食管癌的病理分级、临床分期和淋巴结转移有关.结论 FN、LN、EMMPRIN表达可作为评价食管癌侵袭性及恶性程度的有用指标.     

层粘连蛋白和纤维粘连蛋白在癌组织中的分布及其意义

目的:探讨在浸润、分化程度、组织学类型和转移中,癌组织细胞外基质(LN和FN)的含量改变和分布规律.方法:采用S-P免疫组化染色法观察了257例癌组织的LN和FN的含量分布.染色程度分为(-)、( )和( ),将癌按浸润程度,分化程度、组织学类型和有否转移进行分组.结果:基底膜LN和FN在非浸润癌时连续完整,微浸润区可见分段或缺失,浸润癌时含量明显减少,分布不规则.两者在分化好组和分化差组的表达具有显著性差异(P<0.01).在已分化的各组织学类型之间,LN和FN的表达无明显差异(P<0.05),在转移和未转移组的表达也无明显差异(P>0.05).97%以上的癌间质中存在FN阳性物.结论:基底膜LN和FN是否完整对于鉴别癌是否有浸润有重要的参考价值,其含量随肿瘤细胞分化程度的降低而减少,而与组织学类型和转移无明显关系.间质FN的存在和分布与肿瘤的分化程度、组织学类型及淋巴结转移均无明显关系,可能反映机体对肿瘤的防御和抵抗功能的状态.     

食管鳞癌淋巴管分布及密度与淋巴结转移的关系

目的:探讨食管鳞状细胞癌组织中淋巴管的分布及密度并分析其与淋巴结转移的关系.方法:应用单克隆抗体D2-40检测食管癌巢周围和癌巢中心区以及正常食管组织中淋巴管密度(LVD),分析其与临床病理因素的关系.结果:癌巢周围区LVD明显高于中心区和正常组织(P＜0.01).癌巢周围区LVD与淋巴结转移及分化程度相关(P＜0.05),与浸润深度无关(P＞0.05),癌巢中心区LVD与食管鳞癌分化程度、浸润深度和淋巴结转移均无相关性(P＞0.05).结论:D2-40是淋巴管特异和敏感的标记物,食管癌功能性淋巴管主要存在于食管癌巢周围,检测癌巢周围淋巴管可能是预测淋巴结转移的可靠指标.     

乳腺癌组织中CD44v6和MMP-2的表达及其与淋巴结转移的关系

目的:检测CD44v6、MMP-2在乳腺浸润性导管癌中的表达情况,探讨它们与淋巴结转移的关系。方法:采用免疫组化SP法检测100例乳腺浸润性导管癌中CD44v6、MMP-2的表达情况。结果:CD44v6蛋白在正常乳腺组织及乳腺浸润性导管癌中的阳性表达率分别为12.5%(5/40)、87%(87/100),CD44v6在淋巴结转移组中的阳性表达率(56/60)明显高于无淋巴结转移组(P<0.05)。MMP-2蛋白在正常乳腺组织及乳腺浸润性导管癌中的阳性表达率分别为0(0/40)、94%(94/100),MMP-2在淋巴结转移组中的阳性表达率(59/60)明显高于无淋巴结转移组(P<0.05)。结论:CD44v6、MMP-2蛋白在乳腺浸润性导管癌组织高表达,且均与淋巴结转移有关(P<0.05),联合检测CD44v6与MMP-2有助于综合判断乳腺浸润性导管癌的恶性程度和转移潜能。     

乳腺癌转移相关蛋白mRNA定量表达与临床相关性研究

目的探讨基质金属蛋白酶-2(MMP-2)和纤维粘连蛋白(Fibronectin,FN)mRNA表达量与乳腺癌转移的关系及临床意义。方法采用荧光定量RT-PCR方法检测30例乳腺原发癌和配对淋巴结转移癌以及116例乳腺癌的MMP-2和FN mRNA表达量,分析MMP-2和FN mRNA表达量与乳腺癌转移及临床病理因素相关性。结果30例淋巴结转移癌的MMP-2和FN mRNA表达量低于原发癌,组间比较差异有显著性(P<0.05)。肿瘤直径大、临床分期晚及淋巴结转移数目多的MMP-2和FNmRNA表达量低于肿瘤直径小、临床分期早及淋巴结转移数目少的乳腺癌。MMP-2和FN mRNA表达量与病理类型、组织学分级、雌、孕激素受体及Her-2表达无关。结论随着乳腺癌转移潜能的增加,MMP-2和FN在转录水平的表达下调。     

VEGF-C阳性肥大细胞与乳腺癌淋巴管生成及淋巴结转移的关系

目的 研究乳腺浸润性导管癌中肥大细胞内VEGF-C的表达以探讨MC影响乳腺癌淋巴管生成及淋巴结转移的机制.方法 用免疫组织化学双重染色检查乳腺良性病变和乳腺浸润性导管癌间质中肥大细胞内VEGF-C,用VEGF-C经SP法标记乳腺良性病变和癌组织,用D2-40标记淋巴管并计数.结果乳腺良性病变MC中极少或无蓝黑色VEGF-C颗粒,而乳腺浸润性导管癌MC中均含有较明显,并且前者MC数量(7.80±1.22),明显低于后者MC数量(15.44±2.39),差异有统计学意义(P＜0.01).在浸润性导管癌中,Ⅰ、Ⅱ、Ⅲ级MC数量分别为(10.75±2.61)、(13.70±1.92)、(15.33±0.62),差异亦有统计学意义(P＜0.01).浸润性导管癌有淋巴结转移组的MC数量及癌周边LVD(14.03±2.01,11.21±4.60)明显高于无淋巴结转移组(11.22±0.55,5.78±3.02),差异均有统计学意义(P＜0.01);MC数量与乳腺癌淋巴管数量呈正相关(r=0.18).结论 乳腺癌可诱导MC表达VEGF-C,MC数量与乳腺癌淋巴结转移及淋巴管生成呈正相关性.     

Nm23、IMP3蛋白在乳腺浸润性导管癌组织中的表达及其临床意义

目的 研究nm23、IMP3蛋白在乳腺浸润性导管癌组织中的表达及两者之间的关系,并探讨其临床意义.方法 采用免疫组化S-P法检测nm23和IMP3在乳腺浸润性导管癌组织和癌旁组织中的表达情况,分析两者的相关性,再对nm23、 IMP3蛋白在乳腺浸润性导管癌及癌旁组织中的表达与淋巴结转移之间的关系进行统计学分析.结果 乳腺浸润性导管癌组织中nm23的表达低于癌旁组织(P<0.05);乳腺浸润性导管癌组织中IMP3的表达高于癌旁组织(P<0.05).淋巴结转移者nm23的表达低于无淋巴结转移者(P<0.05);淋巴结转移者IMP3蛋白的表达高于无淋巴结转移者(P<0.05).乳腺浸润性导管癌组织中nm23与IMP3蛋白的表达呈负相关(r=-0.795,P<0.5).结论 Nm23、IMP3蛋白表达在乳腺浸润性导管癌的形成和转移中起着重要作用,可作为预测其浸润转移潜能的重要指标.     

层粘连蛋白在鼻咽癌中的表达及临床意义

目的探讨鼻咽癌中癌细胞及癌周基底膜层粘连蛋白(laminin,LN)的表达情况及临床意义.方法应用免疫组化S-P法对66例鼻咽癌组织蜡块进行层粘连蛋白检测.结果癌细胞LN阳性表达率为45.5%(30/66),癌周基底膜LN阳性表达率为36.4%(24/66);癌细胞LN表达与鼻咽癌T分期相关,与预后呈负相关,而癌周基底膜LN表达与淋巴结转移呈负相关;癌细胞LN阴性癌周基底膜LN阳性病例预后最好.结论层粘连蛋白的表达与鼻咽癌细胞的浸润、淋巴结转移有关,联合监测癌细胞及癌周基底膜LN的表达情况有助于判断鼻咽癌的预后.     

基质金属蛋白酶-2和纤维粘连蛋白mRNA表达与乳腺癌转移相关性的探讨

目的:探讨基质金属蛋白酶-2(MMP-2)和纤维粘连蛋白(Fibronectin,FN)mRNA表达量与乳腺癌转移的关系.方法:采用荧光定量RT-PCR方法检测30例乳腺原发癌和配对淋巴结转移癌的MMP-2和FN mRNA表达量,分析MMP-2和FN mRNA表达量与乳腺癌转移的关系.结果:30例淋巴结转移癌的MMP-2和FN mRNA表达量低于原发癌,组间比较差异有显著性(P＜0.05).MMP-2和FN mRNA表达量呈线性分布,但线性回归和双变量线性相关分析表明两个基因的mRNA表达量无相关性(P＜0.05).结论:MMP-2和FN mRNA表达量在转移癌较原发癌下调.     

结直肠癌组织中纤维连结蛋白的变化

目的 探讨纤维连结蛋白（ＦＮ）在结肠癌和直肠癌组织中的变化及其与结直肠癌生物学行为的关系。方法 采用免疫组化对结肠粘膜活检及结肠癌和直肠癌手术标本进行了ＦＮ的定位观察。结果 ＦＮ见于肠粘膜上皮细胞、部分癌细胞内以及基阍膜、间质中。异型增生上皮细胞ＦＮ染色增强,癌细胞和其基膜ＮＦ减少缺失且与癌细胞分化程度相关,癌间质ＦＮ丰富。结论 ＦＮ可分为上皮细胞型ＦＮ（细胞ＦＮ和基膜ＦＮ）和间质细胞型ＦＮ（间质     

层粘素和纤粘素在胃癌中的分布

采用层粘素（ＬＮ）多抗和纤粘素（ＦＮ）多抗检测７６例胃癌。证实癌细胞分化程度越低和局部浸润能力越强，造成的基底膜（ＢＭ）缺损越严重。证明反应性间质增生与癌肿的浸润转移趋势有关。癌细胞穿透基底膜进入间质，引起间质反应性增生，后者可促进癌细胞的浸润和转移。     

CORRELATION BETWEEN LAMININ AND CATHEPSIN D EXPRESSIONS IN BREAST CARCINOMA

Objective： Laminin is a major glycoprotein component of basement membrance which is an important barrier to tumor cells which must be breeched before metastatic spread can occur. Proteolytic enzymes play an important role in mediating the passage of cancer cells through the basement membrane （BM） and extracellular matrix. We compared the patterns of laminin and cathepsin D （CD） expressions in a range of benign and malignant breast lesions to better understand the process of tumor progression. Methods： One hundred and sixty-two cases of breast samples comprising 18 fibroadeomas, 22 cases of fibrocystic disease, 96 cases of invasive ductal carcinoma and 26 carcinomas with intraductal components were evaluated for laminin and cathepsin D expressions by immunohistocbemical staining. Results： The prevalence of CD positivity in both neoplastic and stromal cell components were significantly higher in higher histological grade tumors compared to lower grades （P〈0.001）. Various severity of BM disruption correlated with histological grade of the carcinomas （P〈0.001）. There was a negative correlation between the laminin expression and CD presence. Conclusion： In the process of cancer cell invasion and metastasis, the basement membrane is disrupted by proteinase secreted by cancer cells, especially by stroma cells of cancer.     

FN1基因表达与人纤维肉瘤肺转移的相关性

目的研究纤维连接蛋白1(FN1)基因表达与人纤维肉瘤肺转移的相关性。方法将HT1080细胞经裸鼠尾静脉注射接种,采集肿瘤浸润组与正常对照组肺组织RNA,合成cDNA,以含1176个人肿瘤相关基因的芯片行cDNA微阵列分析,以实时定量RT-PCR法测定两组FN1表达。利用激光捕获法采集实验组小鼠肿瘤浸润肺组织及周围组织RNA,分别以实时定量RT-PCR法测定FN1表达。结果肺转移组与对照组相比,表达差异基因31个,占2.3%,其中表达下调的基因占0.3%,表达上调的基因占2.3%,FN1表达明显上调。肿瘤浸润肺组织与周围组织相比,FN1表达明显上调。结论FN1基因高表达可能与人纤维肉瘤肺转移有关。     

Correlation between laminin and cathepsin D expressions in breast carcinoma

AIMS AND BACKGROUND: Laminin is a major glycoprotein component of basement membrane which is an important barrier to tumor cells which must be breached before metastatic spread can occur. Proteolytic enzymes play an important role in mediating the passage of cancer cells through the basement membrane (BM) and extracellular matrix. We have compared the patterns of laminin and cathepsin D (CD) expressions in a range of benign and malignant breast lesions to better understand the process of tumor progression. METHODS: One hundred and sixty-two cases of breast samples comprising 18 fibroadenomas, 22 cases of fibrocystic disease, 96 cases of invasive ductal carcinoma and 26 carcinomas with intraductal components were evaluated for laminin and cathepsin D expressions by immunohistochemical staining. RESULTS: The prevalence of CD positivity in both neoplastic and stromal cell components were significantly higher in higher histological grade tumors compared to lower grades (P <0.01). Various severity of BM disruption correlated with histological grade of the carcinomas (P <0.001). There was a negative correlation between the laminin expression and CD presence. CONCLUSIONS: It was confirmed that in a process of cancer cell invasion and metastasis, it could be necessary with the basement membrane disruption by proteinase secreted by cancer cells and especially by stroma cells of cancer.     

纤维连接蛋白及层黏连蛋白在食管癌表达的研究

目的研究纤维连接蛋白、层黏连蛋白在食管癌中的表达及意义。方法采用免疫组化法分别检测60例食管癌患者纤维连接蛋白、层黏连蛋白的表达,正常食管组织标本、不典型增生食管组织标本各15例作为对照。结果在正常食管组织向恶性转化过程中,连续线状表达减少,碎片状表达增加;纤维连接蛋白(FN)、层黏连蛋白(LN)表达与食管癌病理分级、淋巴结转移有关;FN、LN在癌细胞胞质中阳性表达与食管癌病理分级、有无淋巴结转移无关;癌细胞胞质中FN与LN表达之间有明显的正相关性。结论基底膜FN、LN表达可作为临床判断食管癌分化程度及有无转移的有用指标;癌细胞胞质中FN与LN表达之间的正相关性提示在食管癌发展中两者有内在关联性;FN、LN表达异常对食管癌预后评估有一定意义。     

舌鳞癌纤维连接蛋白的表达及意义

目的：探讨纤维连接蛋白在舌鳞癌中的表达及意义。方法;采用免疫组织化学,免疫荧光和火箭免疫电泳技术,分别研究了ＦＮ在体内舌鳞癌发生,发展中和体外培养的舌鳞癌细胞上的表达及舌鳞癌患者血浆中的浓度。结果;基膜ＦＮ的表达与舌上皮恶变及舌鳞癌的分化相关,而与转移无关;体外培养的癌细胞ＦＮ为阳性;舌鳞癌患者中血浆中ＦＮ的浓度也明显高于正常人。     

Expression of fibronectin and tenascin-C mRNA by myofibroblasts,vascular cells and epithelial cells in human colon adenomas and carcinomas

To understand the mechanisms of tissue remodeling during cancer progression, it is important to know the type of cells that actively express extracellular matrix (ECM) proteins. Twenty-nine adenocarcinomas, 5 adenomas and non-neoplastic mucosa samples were therefore investigated to determine their fibronectin (FN) and tenascin-C (TN-C) expression using in situ hybridization (ISH) and immunohistochemical staining. In the non-neoplastic mucosa, no mRNA signals were found. Two of the adenomas demonstrated positive signals in peri-cryptal cells and the vessels. In the cancers, TN-C and FN mRNAs were found in 86% and 96% of the total cases, respectively. The signals were mainly detected in myofibroblasts, labeled with α-smooth muscle actin, in the cancer stroma. TN-C mRNA-positive cells were often observed in localized areas, such as in cancer stroma associated with invading edges and/or in host tissues surrounding the invading cancer front, but rarely in the center of the tumors. FN mRNA-positive cells were more widely spread throughout the cancer stroma, although they were also frequently observed at invading edges. Vascular cells in cancer tissues were also labeled. In 10 specimens, cancer cells themselves expressed FN and/or TN-C mRNA. Comparison with histo-pathological findings revealed positive relationships between the degree of mRNA expression of FN and TN-C and the depth of invasion as well as the frequency of metastasis to lymph nodes. The expression of FN and TN-C by myofibroblasts, vascular cells and cancer cells could be important for the remodeling process of neoplastic tissues during cancer development and progression. Int. J. Cancer 73:10–15, 1997. © 1997 Wiley-Liss, Inc.