沂南地区正常育龄妇女TORCH感染血清学筛查分析

目的探讨正常育龄妇女TORCH感染血清学筛查的意义及不良妊娠史与TORCH感染的相关性.方法酶联免疫捕获法(ELISA),对18039例生育妇女及385例有不良妊娠史的育龄妇女进行TORCH-IgM检测.结果(1)18039例育龄妇女中,总阳性率5.90%.其中HCMV-IgM阳性329例,阳性率1.82%;RV-IgM阳性223例,阳性率1.24%;TOX-IgM阳性184例,阳性率1.01%;HSV-Ⅱ-IgM阳性252例,阳性率1.40%.(2)385例有不良妊娠史的妇女中,总阳性率19.22%.其中HCMV-IgM阳性23例,阳性率5.97%;RV-IgM阳性16例,阳性率4.16%;TOX-IgM阳性14例,阳性率3.64%;HSV-Ⅱ-IgM阳性21例,阳性率5.45%.结论:育龄妇女进行TORCH病原体筛查,宜在孕前期进行,可阻止由TORCH病原体引起的缺陷儿出生,对初筛TORCH-IgM抗体阳性的血清应当复查和确认,避免假阳;有不良妊娠史的妇女TORCH-IgM阳性检出率明显高于正常育龄妇女.     

359例孕前妇女TORCH感染检测的临床意义

目的了解来本院遗传门诊就诊的孕前妇女359例TORCH（TOX、RV、CMV、HSV）感染的情况,探讨孕前筛查TORCH的临床意义,为进一步怀孕提供必要的临床资料。方法用意大利LIAISON全自动定量化学发光仪对359例孕前妇女同时进行TORCH-IgM及IgG抗体的检测。结果TOX-IgM、RV-IgM、CMV-IgM及HSV-IgM的阳性率分别为4%、5.8%、1.5%及8.9%;TOX-IgG、RV-IgG、CMV-IgG及HSV-IgG的阳性率分别为0.5%、76.9%、81.5%及81.0%。结论孕前妇女存在一定的TORCH病原体感染,应加强各病原体的检测与预防,以做好优生优育工作,提高出生人口素质。     

国产化学发光法评估围孕期妇女TORCH感染情况及其IgM假阳性结果的分析

目的 探讨苏州地区围孕期女性TORCH感染情况及流行特点,并对TORCH-IgM假阳性检测结果进行分析,为本地区围孕期女性保健提供参考依据.方法 回顾性分析、统计2018年6月至2020年9月在南京医科大学附属苏州医院就诊的17984例围孕期女性TORCH检查结果,利用国产化学发光免疫分析法(CLIA)对TORCH的IgG以及IgM抗体进行检测,分析感染率.利用ELISA法对129例TORCH-IgM和IgG抗体双阳性血清进行IgG亲和力检测,分析IgM抗体假阳性结果.结果 17984例TORCH检测结果中,IgG抗体阳性率由高到低依次为CMV(97.58％)、HSV-1(89.54％)、RV(78.81％)、HSV-2(10.88％)和TOX(2.83％).IgM抗体阳性率由高到低依次为CMV(1.22％)、HSV-1(1.18％)、HSV-2(0.77％)、RV(0.57％)和TOX(0.36％),TOX感染阳性率最低,CMV感染阳性率最高.RV、CMV和HSV-1感染模式以IgM-/IgG+为主,TOX和HSV-2主要表现为IgM-/IgG-模式.按照季节统计,HSV-1-IgM在冬季阳性率最高(χ2值8.36,P值0.04),TOX-IgM、RV-IgM、CMV-IgM和HSV-2-IgM各季节阳性率差异无统计学意义(χ2值分别为2.51、4.16、1.84和5.12,P值分别为0.48、0.25、0.61和0.16).TORCH-IgG抗体阳性率四季差异均无统计学意义.按照年龄分组统计,<35岁组CMV-IgG、HSV-1-IgG和HSV-2-IgG阳性率明显低于≥35岁组(χ2值分别为12.34、15.04和238.36,均P<0.01),低年龄组TOX-IgG感染率明显高于高年龄组(χ2值为10.01,P<0.01),两年龄组间RV-IgG和TORCH-IgM抗体阳性率差异无统计学意义.化学发光免疫分析法检测129例TORCH-IgM和IgG抗体双阳性标本中,仅19例(14.73％)检测结果为IgG低亲和力.结论 苏州地区围孕期女性TORCH感染普遍,建议育龄女性孕前进行常规筛查,防止TORCH近期感染,预防出生缺陷.对于TORCH-IgM和IgG抗体双阳性结果可进一步联合IgG亲和力检测,排除TORCH感染假阳性.     

孕妇TORCH感染对胎儿的影响

目的　探讨早期诊断TORCH宫内感染及对胎儿的影响。方法　采用酶联免疫吸附法 (ELISA)检测 75 0例妊娠妇女静脉血弓形体 (TOX)、风疹病毒 (RV)、巨细胞病毒 (HCMV)、单纯疱疹病毒 (HSV)简称TORCH的特异性抗体IgM和 4 1例TORCH -IgM阳性的孕妇其新生儿的配对脐血 ,并对孕早中期病理妊娠 (病理组 )与正常妊娠 (对照组 )各 5 0例标本进行对照。结果　孕妇TORCH -IgM阳性率为 10 5 3% ;病理组TORCH -IgM阳性率明显高于对照组 ;TORCH -IgM阳性组其早产、胎膜早破及胎儿宫内发育迟缓发生率显著高于阴性组 ;孕妇TORCH -IgM阳性其垂直传播率分别为TOX -IgM 2 8 5 7% ,RV -IgM 5 0 0 0 % ,HCMV -IgM 5 4 5 5 % ,HSV -IgM 30 77%。结论　 (1)孕期TORCH感染可垂直传播给胎儿 ,是导致流产、死胎、畸形 ,增加产科并发症的重要原因之一。 (2 )ELISA检测孕期TORCH感染有助于早期诊断宫内感染 ,便于临床及时干预。 (3)ELISA监测TORCH感染具有灵敏度高、特异性强、快速简便等优点 ,适合临床推广应用。     

中草药治疗TORCH感染致不良妊娠的实验室和临床研究

目的探讨TORCH感染与不良妊娠结局的关系,寻找降低TORCH感染综合征的药物.方法通过ELISA检测有不良妊娠史患者血清TORCH IgM抗体294例,并对IgM阳性患者32例进行中医药治疗.结果与结论TORCHIgM感染的阳性率为15.99%,TO、RV、CMV、HSV-1、HSV-2的IgM阳性率分别为5.44%、4.42%、6.12%、6.12%、7.14%.无论TORCH系列的哪一种病毒感染,均可导致不良妊娠的发生,妊娠各期TORCH感染的情况大致相同,所不同的只是各种病原体的比例存在差异.中医药可同时抵抗多种病毒感染,中医药孕前早期治疗TORCH感染是预防和减少不良妊娠发生的有效方法.     

大连地区孕前及孕早期妇女TORCH感染情况调查

目的通过测定大连地区育龄妇女血清中TORCH抗体,对其感染的阳性率进行调查,为本地区孕妇保健提供参考依据。方法采用化学发光免疫分析法(CLIA)检测6408例在我院进行孕前筛查和孕12周前普查妇女血清的TORCH抗体。结果 TORCH-IgM抗体总阳性率达16.3%,其中单纯疱疹病毒(Ⅰ+Ⅱ)感染阳性率为9.5%,IgG类抗体中CMVIgG的阳性率最高达86.4%。结论大连地区孕前及孕中期妇女TORCH感染率较高,应积极进行检测做到早发现,早治疗,做好优生优育。     

2900例孕前及孕早期妇女TORCH筛查结果分析

目的通过孕前及孕早期妇女血清中TORCH抗体阳性率的分析,为预防TORCH感染和优生优育工作提供依据。方法采用化学发光免疫分析法对2900例孕前及孕早期妇女血清弓形虫、巨细胞病毒、风疹病毒和单纯疱疹病毒特异性Ig M、Ig G抗体筛查。结果 TORCH Ig M总阳性率达16.97%,其中HSV(Ⅰ+Ⅱ)Ig M阳性率最高,达14.80%。Ig G抗体中CMV Ig G阳性率高达96.12%。结论孕前和孕早期应进行TORCH筛查,是做好优生优育工作,提高出生人口素质的基础。     

乌鲁木齐地区孕妇人细小病毒B19与TORCH感染的比较

目的了解我区孕早期妇女TORCH及人细小病毒B19的感染情况.方法用ELISA法检测185例孕早期妇女血清中特异性 TORCH/HPVB19-IgM抗体.结果感染率分别为弓形体(TOX)2.16%,风疹病毒(RV)1.62%,巨细胞病毒(CMV)1.62%,单纯疱疹病毒Ⅱ型(HSV-Ⅱ)1.08%,人细小病毒B19(HPVB19)8.11%,其HPVB19-IgM抗体阳性率与TORCH各项相比,差异有显著的统计学意义(P＜0.01).结论我区孕妇中人细小病毒B19新近感染率明显高于其它TORCH各项病原体的新近感染率,有必要把HPVB19列入TORCH监测项目中,以提高妊娠质量.     

宁波地区1220例孕妇TORCH感染状况分析

目的了解宁波地区孕妇TORCH感染状况,为预防TORCH感染、降低新生儿出生缺陷发生率提供参考资料。方法采用酶联免疫吸附试验(ELISA)对1220例妊娠妇女进行TORCH-IgM及IgG抗体检测,然后采用病例-对照研究分析TORCH感染与不良妊娠的相关性。结果 TORCH的10项检测结果 ,包括TOX、RUV、CMV、HSV-Ⅰ和HSV-Ⅱ的IgM阳性率分别为2.42%,1.89%,4.26%,9.19%,2.54%,IgG阳性率分别为3.01%,65.4%,90.47%,52.86%,13.3%。本地区不良妊娠孕妇群体中TOX、RUV、CMV、HSV-Ⅰ和HSV-Ⅱ的IgM抗体阳性率分别为12.77%,10.20%,14.29%,21.28%,10.64%与对照组比较差异性显著(P0.01)。结论宁波地区妊娠妇女HSV-Ⅰ感染率最高,其次是CMV。TORCH感染是不良妊娠结局的重要危险因素,应重视TORCH病原体感染的早期筛查和诊治。     

沂南地区TORCH感染与不良妊娠结局的关系

目的探讨沂南地区妊娠期妇女TORCH感染与不良妊娠结局的关系,了解TORCH-IgM感染对孕妇妊娠不良结局的影响。方法采用酶联免疫吸附试验(ELISA)检测方法,通过对孕期发生不良妊娠的466例妇女来站做TORCH四项病原体血清学检测分析,随机抽取同期来站做围产期保健的正常妊娠的妇女466例作为对照组。结果不良妊娠孕妇的TOX-IgM、RV-IgM、CMV-IgM、HSVⅡ-IgM阳性率均明显高于正常妊娠的妇女(P<0.01)。结论 TORCH感染是导致孕妇不良妊娠结局的重要原因之一。因此对有生育计划的育龄妇女孕前进行TORCH-IgM检测是非常有必要的,对检测出TORCH阳性的高危人群采取积极治疗、跟踪随访、优生指导等综合管理措施,能明显降低宫内感染和胎、婴儿传播的机会,提高出生人口素质。     

雷公藤甲素抗肿瘤血管新生的研究进展（英文）

雷公藤甲素(triptolide,TPL)是从中草药雷公藤中提取的一种有效活性物质,已被用来治疗多种疾病,包括系统性红斑狼疮,类风湿性关节炎,肾病综合征等,TPL甚至有很强的抑制肿瘤的活性。近些年的研究显示,TPL具有抗血管新生的能力,TPL不仅可以抑制肿瘤的增殖,诱导细胞的凋亡,还可以抑制肿瘤的转移,可以增加其它化疗药物的抗肿瘤活性。本综述将讨论TPL在抗肿瘤血管新生方面的研究进展,以及初步探讨其潜在的作用机制。     

Changes in activity of neutral proteases in tissues of ground squirrels in the dynamics of hibernation

Total activities of neutral proteases in the cerebral, hepatic, and myocardial tissues of ground squirrel vary during hibernation: in autumn (before hibernation) activities of the enzymes in the brain and myocardium start increasing, while in the liver they do not change. A common feature for all tissues is minimum activity of active neutral proteases in the middle of hibernation month 1 bout, while the maximum activity is recorded before awakening. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 9, pp. 278–280, September, 2008     

Kinetics of decline of maternal measles virus-neutralizing antibodies in sera of infants in France in 2006

The optimal age for measles vaccination is an important health issue, since maternal antibodies may neutralize the vaccine antigen before a specific immune response develops, while delaying vaccination may increase the risk of complicated diseases in infants. However, measles vaccination impacts the duration of protection afforded by transplacental transfer of maternal antibodies: vaccination-induced maternal antibodies disappear faster than disease-induced antibodies. In order to maintain protection against measles in infants, it is important to monitor the dynamics of this phenomenon in vaccinated populations. To assess the current situation in France, a multicenter, prospective seroepidemiological study was conducted in seven French hospitals between October 2005 and January 2007. Maternal measles antibody concentrations from 348 infants 0 to 15 months old were measured using the plaque reduction neutralization assay. Geometric mean concentrations and the percentage of infants with maternal measles antibody concentrations above the protection threshold (≥120 mIU/ml) were assessed according to age. Results show that after more than 20 years of routine measles vaccination in France, maternal measles-neutralizing antibodies decrease dramatically in French infants by 6 months of age, from 1,740 mIU/ml for infants 0 to 1 month old to 223 mIU/ml for infants 5 to 6 months old, and that 90% of infants are not protected against measles after 6 months of age. Infant protection against measles could be optimized both by increasing herd immunity through an increased vaccine coverage and by lowering the age of routine vaccination from 12 to 9 months.     

Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits

In this study, the effect of endosulfan, an organochlorine pesticide, and the ameliorating effect of vitamin C on the livers of New Zealand white rabbits were studied. Livers of the rabbits were examined grossly and histopathologically, and caspase-3 activity was detected by immunohistochemical methods. A total of twenty-four rabbits were divided into four groups (n=6). Rabbits in Group I (END) were daily given a sublethal dose of endosulfan (1 mg/kg bw) in corn oil by oral gavage for 6 weeks. Group II (END+C) received the same dose of endosulfan and additionally Vit C (20 mg/kg bw) every other day during this period. Group III (OIL+C) received corn oil daily by oral gavage and vitamin C every other day for 6 weeks. Group IV (OIL), the control group, received only corn oil daily, by oral gavage throughout the experiment. The concentration of α-endosulfan in the END group was higher in livers (0.102±0.012 ppb) than the β-endosulfan (0.072±0.001 ppb). Decreased accumulation of α and β endosulfan was observed in the END+C group (0.025±0.003 and 0.016±0.002 ppb, respectively) (p<0.0001). The most prominent gross findings at the necropsy were seen in the END group, in which swollen and pale livers were commonly observed. Hemorrhages, degenerations, necrosis, and in some rabbits bile duct hyperplasia were the marked histopathological findings of the END group. Caspase-3 positive reaction was more severe in this group than in the others. An ameliorating effect of Vit C on gross, histopathological and immunohistochemical findings was observed in the END+C group. The results revealed that endosulfan is highly toxic for rabbit livers. However, toxicity was decreased by Vit C treatment, which reduced the accumulation of endosulfan in livers four-fold.