减阻牵张快速牙移动中TGF-β1表达变化对牙周组织改建的影响

目的 探讨减阻牵张快速牙移动中转化生长因子β1（TGF-β1）在牙周组织改建中的作用。方法 20 只 Beagle 犬随机均分为加力 5、10、15 d、加力 15 d 保持 10、90 d 5组。于下颌骨两侧随机分别采用减阻牵张（实验组）和常规正畸方法（对照组）移动第一前磨牙。各组犬分别于预定时间测量牙移动距离并获取第一前磨牙牙周组织块,进行 H-E、苦味酸-天狼星红、免疫组化染色观察。采用SPSS 18.0软件包对数据进行统计学分析。结果 实验组牙移动及牙周改建速度显著快于对照组;2组TGF-β1阳性表达分布区域相似,均于15 d骨生成最活跃阶段达到峰值,但加力各阶段实验组阳性表达显著高于对照组（P<0.05）。结论 与常规正畸方法相比,减阻牵张能明显增加移动牙张力侧牙周组织TGF-β1的表达,加速牙周组织改建。     

改变食物硬度对大鼠生长早期髁突软骨中VEGF、aggrecanase-1和TIMP-3mRNA表达的影响

目的:通过改变食物硬度造成大鼠生长早期颞下颌关节软骨的负荷改变,检测髁突软骨中血管内皮生长因子(vascular endothelial growth factor,VEGF)、聚集蛋白聚糖酶-1(aggrecanase-1)和金属蛋白酶抑制剂因子-3(tissue inhibitor of metalloprotei...     

Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects

ObjectivesPeriodontitis is an infectious disorder that leads to irreversible loss of the surrounding attachment and bone destruction. Genetic polymorphism of cytokines has been suggested to play a role in periodontitis. This case-control study aimed to investigate the relationship between periodontitis and two single nucleotide polymorphisms (SNPs): rs1800629 (-308 G/A) and rs1799964 (-1031 T/C), in the TNF-α gene promoter area.Materials and methodsPeripheral blood was used to prepare genomic DNA from 60 subjects with stage II to stage III periodontitis, as along with 65 control subjects. Polymerase chain reaction and restriction endonuclease digestion were used to genotype TNF-α SNPs.ResultsThe distribution of both genotypes and alleles of TNF-α (-308 G/A) polymorphism did not vary between periodontitis subjects and the controls (P > 0.05). However, the CT genotype and C allele of the TNF-α (-1031 T/C) polymorphism were observed more frequently in the periodontitis subjects than in the controls, while the TT genotype and the T allele were more predominant in the control subjects than in the periodontitis patients (OR: 3.149; 95% CI: 1.494–6.639; P = 0.002 and OR: 2.933; 95% CI: 1.413–6.090; P = 0.003, sequentially).ConclusionThe TNF-α (-308 G/A) polymorphism potentially has no correlation with periodontitis susceptibility, whereas the TNF-α (-1031) CT genotype and C allele might be related to periodontitis among Saudi subjects.     

The effect of transforming growth factor beta one (TGF-β1) on wound healing,with or without barrier membranes,in a Class II furcation defect in sheep

The purpose of this study was to analyse the effect of TGF-β₁ on wound healing in standardized Class II furcation defects of 48 mandibular second premolar teeth in 24 sheep. The experimental design included a control group (carrier only, 25% pluronic F-127), and 2 experimental groups: group A (80 μg/ml TGF-β₁ + carrier) and group B (80 μg/ml TGF-β₁ + carrier covered with a barrier membrane). Sheep were killed either 2 wk or 6 wk after surgery. Mesiodistal sections of the decalcified specimens were quantified histologically using stereology. Percentage volumes of regenerated bone, fibrous connective tissue and cementum were calculated for each furcation defect. Mean values were analysed using multiple ANOVA; p values were calculated using paired and unpaired Student's t-tests. After 2 wk there was more bone in group B than either of the other 2 groups, but this was not statistically significant. By 6 wk more bone was present in group A than in the control group (p<0.02) and also in group B when compared with both group A and the control group (p<0.02 and p<0.44), respectively. In the 4 wk between sampling significantly more bone had formed (group A <0.05 and group B p<0.003, respectively). A negative correlation existed between volumes of bone and fibrous connective tissue and no significant differences between the volumes of cementum were evident between any of the groups. This study demonstrated that TGF-β₁ encouraged bone regeneration in Class II furcation defects in sheep, an effect enhanced by the presence of a barrier membrane. This is the first report on the use of TGF-β₁ in conjunction with GTR in periodontal defects.     

The effect of transforming growth factor beta one (TGF-β1) on wound healing,with or without barrier membranes,in a Class II furcation defect in sheep

The purpose of this study was to analyse the effect of TGF-β₁ on wound healing in standardized Class II furcation defects of 48 mandibular second premolar teeth in 24 sheep. The experimental design included a control group (carrier only, 25% pluronic F-127), and 2 experimental groups: group A (80 μg/ml TGF-β₁+ carrier) and group B (80 μg/ml TOF-β₁+ carrier covered with a barrier membrane). Sheep were killed either 2 wk or 6 wk after surgery. Mesiodistal sections of the decalcified specimens were quantified histologically using stereology. Percentage volumes of regenerated bone, fibrous connective tissue and cementum were calculated for each furcation defect. Mean values were analysed using multiple ANOVA; p values were calculated using paired and unpaired Student's t tests. After 2 wk there was more bone in group B than either of the other 2 groups, but this was not statistically significant. By 6 wk more bone was present in group A than in the control group (p<0.02) and also in group B when compared with both group A and the control group (p<0.02 and p<0.44), respectively. In the 4 wk between sampling significantly more bone had formed (group A <0.05 and group B p<0.003, respectively). A negative correlation existed between volumes of bone and fibrous connective tissue and no significant differences between the volumes of cementum were evident between any of the groups. This study demonstrated that TGF-β₁ encouraged bone regeneration in Class II furcation defects in sheep, an effect enhanced by the presence of a barrier membrane. This is the first report on the use of TGF-β₁ in conjunction with GTR in periodontal defects.