高效液相色谱法同时测定血浆中的肾上腺素和去甲肾上腺素

为同时测定人血浆中肾上腺素和去甲肾上腺素浓度,建立了高效液相色谱－电化学检测方法,采用ＹＷＧ－Ｃ１８分析柱,３,４－二羟在苄胺作内标,以０．０５ｍｏｌ／Ｌ醋酸盐结冲液（ｐＨ３．０）－甲醇－０．０５ｍｏｌ／Ｌ ＥＤＴＡ－Ｎａ２（７８：２０：２）为流动相,每１Ｌ流动相含ＳＤＳ２００ｍｇ,流速为１．０ｍｌ／ｍｉｎ。去甲肾上腺素和肾上腺素及内标的保留时间分别为７．３、８．３和１０．４分钟,线性范围均为３１     

RP—HPLC法测定尼莫地平输液含量及其初步稳定性的研究

采用ＲＰ－ＨＰＬＣ法测定了尼莫地平输液的含量，并对其光、热稳定性进行了研究。采用用Ｓｐｈｅｒｉｓｏｒｈ Ｃ１８柱，以甲醇－０．０５ｍｏｌ／ＬＮａＨ２ＰＯ４（７：３，Ｖ／Ｖ）（以二乙胺调节ＰＨ至４．８）为流动相，２３７ｎｍ检测。该方法专属性好，且、简便。光、热稳定性研究结果表明：输液对光不稳定，对热较稳定，但随加热时间的延长也会逐渐分解。     

HPLC法测定牛黄清心丸中甘草酸含量

用ＨＰＬＣ法测定牛黄清心丸中甘草酸的含量。采用ＯＤＳ－５柱,４．６ｍｍ＊１５０ｍｍ,流动相为０．０５ｍｏｌ／ＬＫＨ２ＰＯ４（ｐＨ＝２．５）－ＭｅＣＮ（２１：１０）,流速为１．０ｍＬ／ｍｉｎ,检测波长２５４ｎｍ。检测量在０．５６４－２．０６８μ之间关系良好。     

HPLC法测定经皮渗透接受液中双氯灭痛的浓度

采用ＲＰ－ＨＰＬＣ以４＇－羟基－５－氯双氯灭痛为内标，测定了经皮渗透接受液中双氯灭痛的含量。考察了流动相组成、ｐＨ值等对双氯灭痛色谱行为的影响情况。方法简便准确，回收率为９８．４４％～１０２．３％，ＲＳＤ＜２．４％，双氯灭痛浓度在０．５～１６μｇ／ｍｌ范围内，线性关系良好（ｒ＝０．９９９９），最低检测限５０ｎｇ／ｍｌ。色谱条件为：Ｃ（１８）Ｈｙｐｅｒｓｉｌ（５μｍ，１００ｍｍ×４．６ｍｍ）不锈钢柱，甲醇ｐＨ７．０磷酸盐缓冲液（０．０５ｍｏｌ／Ｌ）（５６：４４）作流动用，检测波长２８０ｎｍ，进样量２０μｌ。     

RP—HPLC法测定异长春花碱注射液的含量

目的：建立了ＲＰ－ＨＰＬＣ法分离和测定异长春花碱注射液的含量。方法：ＳｐｈｅｒｉｓｏｒｂＣ１８柱，流动相０．０５ｍｏｌ／ＬＫＨ２ＰＯ４（用Ｈ３ＰＯ４调pＨ为４． ０）－乙晴（６０：４０），检测波长：２６８ｎｍ。结果：异长春花碱的线笥范围为０～１００ｕｇ／ｍｌ，ｒ＝０．９９９９，平均回收率为１００。９８％（n＝６），ＲＳＤ ＝３．７３％。结论：该法不需经提取分离，稀释后直接进样，简便，准确，适于生产中使     

高效液相色谱法测定氟氧头孢的血药浓度

建立了测定氟氧头孢血药浓度的ＨＰＬＣ法。采用Ｈｙｐｅｒｓｌｌ Ｃ１８色 柱,以甲醇－乙腈－水－磷酸－三乙胺（１６：６７８：０．２８：０．４）为流动相,流速为１．０ｍｌ／ｍｉｎ,检测波长２７０ｎｍ灵敏度０．０５ＡＵＦＳ。该法线性范围为０．２５～１００μｇ／ｍｌ（ｒ－０．９９９８）,回事率大于９３％,日内、日间ＲＳＤ小于８．０％。     

HPLC法测定石刁柏中的蜀葵氨酸含量

采用Ｚｏｒｂａｘｒｘ－Ｃ１８色谱柱，以０．０５ｍｏｌ／Ｌ醋酸钠甲醇为流动相，邻苯二甲醛－２巯基乙醇做柱后衍生剂，利用荧光检测器的高效液相色谱法石刁柏中的蜀直接分离分析，方法简便，快速，重现性好，回收率为９６．４％。     

人血浆萘哌地尔浓度的高效液相色谱法测定

目的 研究人血浆萘哌地尔的高效液相色谱法（ＨＰＬＣ）测定。方法 血浆样品依次用６ｍｏｌ／ＬＨＣｌ，ＮａＯＨ处理，乙醚提取２次，色谱分析柱为粒径５μｍ，填料为ＥｃｏｓｐｈｅｒｅＣ１８，内标为美托洛尔，流动相为甲醇－乙腈－水－０．０２ｍｏｌ／ＬＫＨ２ＰＯ４（５５：２０：２２：３）ｐＨ４．１０。荧光检测器激发波长２９０ｎｍ，发射波长３４０ｎｍ。结果 最低检测浓度为１．０ｎｇ／ｍｌ天内及天间的变异系数为１     

高效液相色谱法测定左氟沙星片剂的含量

采用反相高效液相色谱法测定左氟沙星片剂的含量，以ＷａｔｅｒｓＮｏｖａ－ＰａｋＣ１８为固定相，１０ｍｍｏｌ／ＬＫＨ２ＰＯ４－１０ｍｍｏｌ／Ｌ溴化四丁铵－乙腈（４５：４５：１０，磷酸调节ＰＨ２．８）为流动相，紫外检测波长２９３ｎｍ，平均回收率为９８．９９％，ＲＳＤ为０．６２％，方法简便，准确。     

反相高效液相色谱法测定Ca~（2＋），Mg~（2＋）－ATPase活力的方法

应用反相ＨＰＬＣ方法测定了大鼠心肌Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力。高效液相色谱条件：Ｗａｔｅｒｓ－４８０高效液相色谱仪，ＷａｔｅｒｓＣ－１８高效反相色谱柱，流动相为０．０６６７ｍｏｌ／ＬＫＨ２ＰＯ４／Ｋ２ＨＰＯ４缓冲液（ｐＨ５．９２），流速１．５ｍｌ／ｍｉｎ，柱压３０００Ｐａ，检测波长２５４ｎｍ。方法简便，快速，灵敏度高，重复性好。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.