腹部环形按摩结合早期游泳对新生儿黄疸症状、胆红素及炎性因子水平的影响

目的探讨腹部环形按摩结合早期游泳在新生儿黄疸护理中的应用价值。方法选择2016年7月至2018年3月深圳市龙华区中心医院诊治的104例新生儿黄疸患儿为研究对象,采取随机数字表法将其分为观察组和对照组,每组52例患儿。观察组患儿在对照组基础上,实施腹部环形按摩与早期游泳;对照组患儿行常规护理。观察两组患儿排便和黄疸消退情况,干预前后黄疸指数、胆红素和炎性因子变化。结果观察组患儿胎便初排时间、胎便转黄时间、黄疸消退时间均明显短于对照组患儿,每日排便次数、每日进奶量均明显多于对照组患儿,其差异均具有统计学意义(均P<0.05)。干预前,两组患儿黄疸指数、血清总胆红素水平比较,其差异均无统计学意义(均P>0.05);干预后,观察组患儿上述指标水平均明显低于对照组患儿,其差异均具有统计学意义(均P<0.05)。干预前,两组患儿炎性因子水平比较,其差异均无统计学意义(均P>0.05);干预后,观察组患儿血清CRP、IL-2、IL-6水平明显低于对照组患儿,其差异均具有统计学意义(均P<0.05)。结论在新生儿黄疸护理中,应用腹部环形按摩结合早期游泳,可促进黄疸症状消退,降低胆红素和炎性因子水平,值得推广。     

硬皮病小鼠皮损TGF-β_1 I,III型胶原含量及其相关性分析

目的探讨转化生长因子(TGFβ1)在硬皮病发病中的作用及其与COLI,COLIII含量的相关性。方法采用免疫组化技术检测7例硬皮病小鼠模型的皮损中TGFβ1蛋白及I,III型胶原蛋白含量,以7例正常小鼠皮肤组织作为对照(不加BLM的PBS液处置);并对I,III型胶原及TGFβ1的含量进行比较和相关分析。结果TGFβ1及I,III型胶原在硬皮病小鼠皮损的含量均高于对照组(P<0.001);相关分析表明I型胶原的含量与TGFβ1的含量呈正相关(P<0.05)。结论在硬皮病小鼠皮损中有较多的TGFβ1,且硬化皮肤中TGFβ1与I型胶原的含量有显著相关性。提示其在小鼠皮肤硬化过程中起一定作用,可能与硬皮病的病理纤维化的形成有关。     

Comparison of the diagnostic accuracy of whole‐body MRI and whole‐body CT in stage III/IV malignant melanoma

Background: Malignant melanoma (MM) is dramatically increasing in light‐skinned populations worldwide. Staging and regular follow‐up examinations are essential. The purpose of this study was to compare the diagnostic accuracy of whole‐body MRI with the standard diagnostic algorithm (whole‐body CT and brain MRI) in patients with stage III/IV MM. Patients and methods: A group of 50 consecutively admitted patients with stage III/IV MM were included in the study. Whole‐body CT and brain MRI scans were performed. Additionally, all patients underwent a whole‐body MRI (1.5 Tesla Magnetom Avanto, Siemens Healthcare Sector, Erlangen). The findings were compared on a lesion‐by‐lesion basis as part of clinical routine follow‐up. Results: 33 patients received a follow‐up CT and were evaluated. Overall, 824 lesions were detected. The sensitivity of whole‐body MRI was observer‐dependent. MRI was slightly less sensitive than CT according to the findings of the two most experienced observers (73.4 % vs. 78.2 %, p = 0.0744). CT was significantly more sensitive in the detection of small (1–5 mm) pulmonary nodules (2.9 % vs. 66.9 %, p < 0.0001). Yet overall, MRI was significantly more specific than CT (83.4 % vs. 50.4 %, p < 0.0001). Conclusions: Whole‐body MRI in compliance with standard requirements for the observers (high level of experience) should be considered as an appropriate alternative to CT without ionizing radiation, particularly for young patients with advanced MM.     

Lack of co-ordinate expression of the alpha1(I) and alpha1(III) procollagen genes in fibroblast clonal cultures

BACKGROUND: Several extracellular matrix genes, most notably alpha1(I) and alpha1(III) procollagen, are reported to be co-ordinately expressed in cultures of dermal fibroblasts. However, it remains unclear whether the expression of these genes is truly co-ordinate or whether it may be the result of averaging the phenotypic expression of different fibroblast subpopulations present within each culture. Objectives To determine by Northern analysis the correlation between alpha1(I) and alpha1(III) procollagen mRNA levels in clonal populations of human dermal fibroblasts. METHODS: As previously described, clonal cultures were derived from parent strains of human dermal fibroblasts by a microscopically controlled dilution technique and by stimulation of single cells with low oxygen tension in the early phases of clonal growth. RESULTS: In agreement with previous reports, we found that baseline steady-state levels of alpha1(I) procollagen mRNA were co-ordinately regulated with the alpha1(III) procollagen mRNA in 26 parent strains (r = 0. 9003; P < 0.0001). However, this close correlation between the expression of these two procollagen chains was absent in a total of 40 unselected clonal strains derived from four of the parent cultures (r = 0.5745; P < 0.0001). Moreover, this intrachain heterogeneity in alpha1(I) and alpha1(III) procollagen mRNA levels in clonal cultures was statistically significant from that measured in parent strains (P = 0.0016). CONCLUSIONS: alpha1(I) and alpha1(III) procollagen mRNA levels in clonal cultures do not show the tight co-ordinate regulation observed in non-clonal cultures, suggesting that these two genes operate under different sets of regulatory controls. This clonal heterogeneity may provide additional flexibility to the process of tissue repair and fibroblast clonal expansion.