Quadriceps tendon-patellar bone autograft for anterior cruciate ligament reconstruction: a technical note

The quadriceps tendon autograft can be used for primary and revision anterior cruciate ligament (ACL) reconstruction. Despite several successful clinical reports, graft fixation issues remain, and the ideal technique for fixation continues to be controversial. We present a technique of ACL reconstruction with quadriceps tendon autograft (QTA) using a patellar bone block. The tendon end is fixed in the femoral tunnel and the bone plug in the tibial tunnel using reabsorbable interference screws. The advantages of this technique are related to the increase in stiffness of the graft, the achievement of a more anatomic fixation, and a reduction in synovial fluid leakage.     

Single–Achilles Allograft Posterior Cruciate Ligament and Medial Collateral Ligament Reconstruction: A Technique to Avoid Osseous Tunnel Intersection,Improve Construct Stiffness,and Save on Allograft Utilization

We present a method for single–Achilles allograft medial collateral ligament (MCL) and posterior cruciate ligament (PCL) reconstruction that eliminates the risk of tunnel intersection, stiffens the construct, and maximizes utilization of allograft tissue. An Achilles tendon allograft is prepared with an 11- to 12-mm bone plug with a gradual taper to 7 mm over approximately 15 cm. A transtibial PCL tunnel is created under fluoroscopic and arthroscopic guidance. The femoral tunnel is prepared in an “outside-in” fashion under direct arthroscopic visualization, originating at the anatomic origin of the MCL on the medial epicondyle and entering the joint at the anatomic origin of the anterolateral bundle of the PCL. The Achilles graft is pulled into the joint through the tibial tunnel and routed into the femoral tunnel so that the soft tissue exits at the medial epicondyle. The bone plug is fluoroscopically guided to the posterior aperture of the tibial tunnel and fixed with a bioabsorbable interference screw. The pretensioned graft is fixed in the femoral tunnel via interference screw fixation with the knee in 90° of flexion. The isometric position of the MCL insertion is identified with a K-wire isometer, and the graft is fixed in place at this point by use of an interference screw or screw and washer.     

Evaluation of bone incorporation of patellar tendon autografts and allografts for ACL reconstruction using CT

Anterior cruciate ligament (ACL) reconstruction is a relatively common orthopedic procedure, with patellar tendon frequently a graft source. However, controversy exists regarding the decision to use autograft or allograft patellar tendon tissue. This experimental study used computed tomography (CT) to compare the percentage of bone graft incorporation following ACL reconstruction using autografts and allografts. Fifty consecutive patients undergoing ACL reconstruction were included in the study. The tibial bone plug was imaged with CT 1 week, 2 months, and 5 months postoperatively. Four images from each completed scan were analyzed for percentage of incorporation of the bone graft. The results of autograft and allograft incorporation for each of the time intervals were compared. No statistically significant difference was found in the amount of bone incorporation at the tibial bone plug 1 week, 2 months, and 5 months. Clinical concerns regarding slower or less complete healing of allograft bone tissue compared to autograft are not supported with regard to the grafts studied.     

Arthroscopic Reconstruction of the Posterior Cruciate Ligament Using Tibial-Inlay and Double-Bundle Technique

Biomechanical research has suggested that the double-bundle and tibial inlay technique is superior to the single-bundle and the transtibial tunnel method for posterior cruciate ligament (PCL) reconstruction. A combination the posterior tibial inlay and femoral double-bundle technique is thought to be an ideal method for PCL reconstruction. Recently, we successfully performed arthroscopic PCL reconstruction using the tibial-inlay and double-bundle technique. Achilles tendon–bone allograft is used and the bone plug for the arthroscopic tibial inlay fixation is designed in a cylindrical shape and perpendicular to the fiber texture of the Achilles tendon. Achilles tendon is manually split into deep and superficial layers to reconstruct anterolateral and posteromedial bundles as the natural insertion of PCL. The intra-articular lengths of each bundle between tibial tunnel and 2 femoral tunnels are measured to achieve fixation of the graft to the original PCL attachment. After tibial bone plug fixation with an absorbable interference screw and additional suture anchoring, the anterolateral bundle is fixed in a reduction position with the knee in 90° of flexion and the posteromedial bundle is fixed nearly in extension. This procedure makes it possible not only to reproduce the original concept of PCL tibial inlay graft arthroscopically without posterior arthrotomy, but also to achieve a more anatomic PCL reconstruction of the 2 bundles.     

A New Harvest Site for Bone Graft in Anterior Cruciate Ligament Revision Surgery

During revision anterior cruciate ligament (ACL) surgery, femoral interference screws frequently require removal. This may lead to significant tunnel widening and possible graft fixation failure as a result. Solutions include drilling the revision tunnel in a different location, using stacked interference screws, or using bone graft to fill the defect. Autogenous iliac crest graft and allograft are both used, but there are significant comorbidities associated with each. We developed a new technique for harvesting autogenous bone graft that avoids many of the complications associated with other graft sources. By use of the existing surgical incision from the initial harvest of the bone–patellar tendon–bone autograft, bone from the medial tibial metaphyseal safe zone is harvested via an OATS tube harvester (Arthrex, Naples, FL). A bone plug 1 mm larger in size than the femoral defect is harvested and arthroscopically inserted via a press-fit technique. At 3 months after bone grafting, patients undergo revision ACL reconstruction. The proximal tibial metaphysis is a safe bone graft harvest site in revision ACL surgery and offers an effective method for filling large bony defects, allowing anatomic reconstruction of the ACL after bone healing has occurred. Furthermore, it eliminates the problems associated with allograft or use of a remote graft donor site.     

All-inside anterior cruciate ligament reconstruction using semitendinosus tendon and soft threaded biodegradable interference screw fixation

A modification of anterior cruciate ligament (ACL) reconstruction using a minimally invasive and endoscopic all-inside technique is presented. Both the femoral and tibial socket are approached through the joint so that there is no open tibial tunnel, which otherwise often causes significant pain and discomfort during early rehabilitation. The autologous semitendinosus tendon is harvested with a bone plug attached to its tibial insertion. The triple-stranded semitendinosus tendon is looped around the adjacent bone plug and fixed at the original tibial attachment site of the ACL using a soft threaded biodegradable poly-(D,L-lactide) interference screw. The screw is inserted endoscopically in an inside-out direction. In the femoral socket the graft is fixed without a bone plug directly to the tunnel wall using the biodegradable screw. The free part of the graft is thus not longer than the intra-articular distance, which may increase stiffness of the construct.     

Femoral interference screw placement through the tibial tunnel: A radiographic evaluation of interference screw divergence angles after endoscopic anterior cruciate ligament reconstruction

Fifty patients who underwent single-incision arthroscopically assisted anterior cruciate ligament (ACL) reconstruction using bone—patellar tendon—bone autograft were reviewed. All procedures were performed using a technique for placement of the femoral interference screw that reduced the divergence between the femoral interference screw and the femoral bone plug. This technique includes securing the femoral interference screw by placing the screw driver through the tibial tunnel, anterior to the tibial bone plug. Using this technique, 8% of our patients had significant femoral bone plug and interference screw divergence: however, no patients had a divergence angle of greater than 15° in either the anteroposterior or lateral planes. None of our patients were noted to have divergence angles consistent with decreased pullout strength. We had no complications caused by screw driver placement, and we did not experience any graft injury. We believe that adequate femoral bone plug and interference screw alignment can be achieved by using this technique during single-incision endoscopic ACL reconstruction.     

Initial fixation strength of modified patellar tendon grafts for anatomic fixation in anterior cruciate ligament reconstruction.

Recently it has been shown that anatomic tibial graft fixation in anterior cruciate ligament (ACL) reconstruction is preferable in order to increase isometry and knee stability. To facilitate anatomic patellar tendon graft fixation, customized graft length shortening is necessary. The purpose of this study was to compare the initial fixation strength of four different shortened patellar tendon grafts including three bone plug flip techniques and direct patellar tendon-to-bone interference fit fixation in a model with standardized bone density. Ninety calf tibial plateaus (22 to 24 weeks old) with adjacent patella and extensor ligaments were used. Tendon grafts were shortened by flipping the bone plug over the tendon leaving a tendon-tendon-bone (TTB) construct and, as the first modification in the opposite direction resulting in a tendon-bone-tendon (TBT) construct. The second modification consisted of the TBT construct with interference screw position at the lateral aspect of the bone plug (TBTlat). As the fourth modification the tendon graft was directly fixed (Tdirect) with an interference screw. In addition, a round-threaded titanium (RCI; Smith & Nephew DonJoy, Carlsbad, CA), a round-threaded biodegradable screw (Sysorb; Sulzer Orthopedics, Münsingen, Switzerland), and a conventional titanium interference screw (Arthrex Inc, Naples, FL) were compared. We found that TTB (mean 441 N for biodegradable screw, 357 N for RCI screw, 384 N for conventional screw) and TBT (mean 407 N for biodegradable screw, 204 N for RCI screw, 392 N for conventional screw) construct fixation achieves comparable fixation strength, although failure in the TTB was due to tendon strip off at its ligamentous insertion. The highest failure load was found in TBTlat fixation (mean 610 N for biodegradable screw, 479 N for RCI screw). Therefore, this technique should be recommended when using a tendon flip technique. The failure load for Tdirect fixation (mean 437 N for biodegradable screw, 364 N for RCI screw) was similar to that of TTB and TBT fixation, which may indicate that a patellar-tendon graft harvested without its patellar bone plug and directly fixed with an interference screw is equivalent to a flipped graft. This may additionally reduce harvest site morbidity and eliminates the risk of patellar fracture. The fixation strength of round-threaded biodegradable and conventional titanium interference screws was similar, whereas that of round-threaded titanium screws was significantly lower in the patellar tendon flip-techniques. However, it should be taken into consideration that round-threaded titanium screws are proposed for direct tendon-to-bone fixation.     

Graft fixation issues in kneeligament surgery

The popularity of soft tissue grafts, particularly the semitendinosus and gracilis, has raised several issues with regard to graft fixation in cruciate ligament reconstruction. What is the force required by activities of daily living and a progressive rehabilitation program? Does soft tissue fixation alter the biological process of graft incorporation compared with the use of a bone plug? This article reviews the mechanical properties and use of fixation devices. Interference screw fixation of a patella tendon bone plug has been clinically reliable. Relative ease of fixation, acceptable initial strength, and fixation close to joint surfaces contribute to the popularity of cruciate ligament reconstruction with patella tendon bone plug. Biodegradable interference screw fixation of a bone plug is a reasonable alternative to metal screw fixation, which has several disadvantages. Many forms of tendon fixation are placed at a distance from the joint and rely on linkage materials, resulting in graft tunnel shear forces and possible tunnel expansion. Direct interference screw fixation may alleviate these detractions, but ultimate failure at time zero, particularly tibial fixation, may not allow for a progressive rehabilitation program, which our patients currently benefit from with interference fixation of a bone plug. Diminishing individual fiber movement within the tendon graft and the elimination of linkage materials will improve future soft tissue fixation.     

Anatomically Oriented Anterior Cruciate Ligament Reconstruction with a Bone–Patellar Tendon–Bone Graft via Rectangular Socket and Tunnel: A Snug-fit and Impingement-Free Grafting Technique

An anterior cruciate ligament (ACL) reconstruction technique is described to place bone–patellar tendon–bone (BPTB) graft in an anatomically oriented fashion to mimic the 2 bundles of the normal ACL, based on the concept of twin tunnel ACL reconstruction, to maximize the graft-tunnel interface. In this technique, the attached bone plug is introduced into a rectangular femoral socket via a halfway rectangular tibial tunnel for the anterior portion of the graft to function as the anteromedial bundle and for its posterior portion to behave as the posterolateral bundle. A snug fitting of the graft is achieved not only at the femoral socket, but also in the tibial tunnel.     

Technical Note: Double tibial tunnel using quadriceps tendon in anterior cruciate ligament reconstruction

Summary: To avoid complications related to the use of patellar tendon and hamstring (semitendinosus and gracilis) tendon and to create a more anatomic reconstruction, we present a new technique based on the use of quadriceps tendon placed in a single half femoral tunnel and double tibial tunnels. The graft, harvested by a central longitudinal incision, possesses the following characteristics: (1) a bone plug 20 mm long and 10 mm in diameter; (2) a tendon component 7 to 8 cm long, 10 mm wide, and 8 mm thick; and (3) division of the tendon longitudinally into 2 bundles while maintaining the patellar insertion. Every bundle has a width and thickness of approximately 5 mm and 8 mm, respectively. The total length of the graft is 9 to 10 cm. A 10-mm half femoral tunnel is drilled through a low anteromedial portal with the knee flexed at 120°. A suture loop is left in place in the half tunnel. A double tibial tunnel is drilled in a convergent manner (from outside to inside) obtaining an osseous bridge between the 2 tunnels. Two suture loops are passed trough the tibial tunnels and retrieved in a plastic cannula (10 mm) positioned in the anteromedial portal to allow the passage of the 2 bundles in the tibial tunnels. The suture loop left in the half tunnel permits the transportation of the bone plug in the femoral tunnel. Fixation is achieved by an interference screw at the femoral side and by 2 absorbable interference screws (1 for each tunnel). The advantages of this technique are a more cross-sectional area (80 mm²), greater bone-tendon interface, and a more anatomic reconstruction. Theoretically, easier bone incorporation, decreased windshield wiper and bungee effect, fewer donor site problems, and less tunnel enlargement can also be possible.     

All-Inside Anterior Cruciate Ligament Reconstruction

Anterior cruciate ligament (ACL) reconstruction is one of the most commonly performed knee operations. An “all-inside” technique creates bone sockets for ACL graft passage, as opposed to more traditional full bone tunnels, and typically incorporates suspensory fixation instead of screw fixation to secure the graft. This technique may be indicated for any ACL reconstruction surgery, where adequate bone stock exists to drill sockets and to use cortical fixation. The technique may be used with all soft tissue, as well as bone plug ACL grafts and autograft hamstring or quadriceps tendon; most allograft tendon options may be performed with an all-inside technique. Advantages include anatomic tunnel/socket placement, decreased postoperative pain and swelling, minimal hardware, appropriate graft tensioning and retensioning, and circumferential graft to bone healing. Tips for successful all-inside surgery include matching graft diameter to socket diameter, drilling appropriate length sockets based on individual graft length, so as not to “bottom out” the graft and confirming cortical button fixation intraoperatively. Potential complications include graft-socket mismatch, full-tunnel reaming, and loss of cortical fixation. Multiple studies have shown the all-inside technique to have similar or superior biomechanical properties and clinical outcomes compared to the more traditional full-tunnel ACL reconstruction techniques.     

Flipped patellar tendon autograft anterior cruciate ligament reconstruction.

To determine the efficacy of an anterior cruciate ligament (ACL) graft that customizes length and facilitates anatomic outlet fixation, a prospective study of the "flipped" patellar tendon autograft ACL reconstruction began in 1995. This technique shortens the tendon portion to match the intra-articular length by rotating 1 bone plug 180 degrees proximally onto the tendon, thus flipping the bone plug over its ligamentous insertion. Bioscrews (poly L-lactic acid; Linvatec, Largo, FL) secured the grafts. All patients undergoing this procedure with a minimum 21 months follow-up were reviewed. Preoperative and postoperative Tegner, Lysholm, and IKDC activity scores, and Lachman and pivot shift tests were obtained. Postoperative KT testing and radiographs were obtained. Fifty patients were followed-up for an average of 28 months (range, 21 to 39 months). Average patient age was 34 years (range, 16 to 52 years). Tegner scores increased from 2.0 preoperatively to 6.0 postoperatively. Lysholm scores increased from 46 preoperatively to 93 at follow-up, with 86% excellent (66%) or good (20%). IKDC activity scores increased from 3.1 preoperatively to 1.7 postoperatively. KT manual-maximum difference at follow-up averaged 0.7 mm, with 74% less than 3-mm, 18% 3- to 5-mm, and 8% greater than 5-mm difference. Postoperative Lachman results were 0 in 45 patients and 1+ in 5 patients. Postoperative pivot shift was absent in all but 1 patient. Full extension was achieved in all cases and flexion averaged 136 degrees with no patient having less than 120 degrees flexion. No lytic bone changes or tunnel widening were seen. The flipped patellar tendon autograft reduces graft length to its intra-articular portion, increasing graft stability, isometry, and stiffness, and avoiding tunnel graft mismatch with clinically excellent results.     

Graft failure versus graft fixation in ACL reconstruction: histological and immunohistochemical studies in rabbits

The causes of graft failure after anterior cruciate ligament (ACL) reconstruction are multifactorial including the methods of graft fixation. The purpose of this study was to examine the ACL graft failure in three different methods of graft fixations including interference screw fixation, suture-post fixation and combined interference screw and suture-post fixation. We hypothesized that the fixation method after ACL reconstruction can affect the graft healing in tibial tunnel. Eighteen New Zealand white rabbits were categorized into three groups according to the method of fixation in unilateral ACL reconstruction with long digital extensor autograft. Histological examination demonstrated that the combined fixation and suture-post fixation groups showed significantly better integration between tendon and bone (P = 0.04). In immunohistochemical analysis, the combined fixation and suture-post fixation groups showed significantly higher BMP-2 and VEGF expressions than interference screw (P < 0.01). The tendon–bone healing after ACL reconstruction was affected by the method of graft fixation. Combined fixation with interference screw and suture-post reduced graft-tunnel micromotion and improved the graft healing in tibial tunnel.     

Arthroscopic anterior cruciate ligament reconstruction with quadriceps tendon composite autograft

At present, no single graft option clearly outperforms another. Autografts (patellar tendon, hamstring) and allografts (Achilles tendon, patellar tendon) are the grafts most often used. However, each grafts has advantages and disadvantages. Quadriceps tendon graft for anterior cruciate ligament reconstruction is not new, but an alternative composite graft is introduced here that consists of quadriceps tendon–patellar bone and bone obtained from a coring reamer used to create the tibial tunnel. This composite graft retains reduced morbidity while allowing the secure bone-to-bone fixation associated with bone–patellar tendon–bone graft.Arthroscopy: The Journal of Arthroscopic and Related Surgery, Vol 17, No 5 (May-June), 2001: pp 546–550     

One-incision endoscopic technique for posterior cruciate ligament reconstruction with quadriceps tendon–patellar bone autograft

Quadriceps tendon–patellar bone autograft is an alternative graft choice for posterior cruciate ligament (PCL) reconstruction. A 2-incision technique with outside-in fixation at the femoral condyle is generally used. In this article, we describe a 1-incision endoscopic technique for PCL reconstruction with quadriceps tendon–patellar bone autograft. The graft consists of a proximal patellar bone plug and central quadriceps tendon. The bone plug is trapezoidal, 20 mm long, 10 mm wide, and 8 mm thick. The tendon portion is 80 mm long, 10 mm wide, and 6 mm thick, including the full-thickness of the rectus femoris and partial thickness of the vastus intermedius. Three arthroscopic portals, including anteromedial, anterolateral, and posteromedial, are used. All procedures are performed in an endoscopic manner with only 1 incision at the proximal tibia. At the femoral side, the bone plug is fixed by an interference screw. At the tibial side, the tendon portion is fixed by a suture to a screw on the anterior cortex and an interference bioscrew in the posterior tibial tunnel opening. Quadriceps tendon autograft has the advantages of being self-available, allowing for easier arthroscopic technique, and providing comparable graft size. The 1-incision technique provides a simple reconstruction method for PCL insufficiency without a second incision at the medial femoral condyle.Arthroscopy: The Journal of Arthroscopic and Related Surgery, Vol 17, No 3 (March), 2001: pp 329–332     

The “N + 7 Rule” for tibial tunnel placement in endoscopic anterior cruciate ligament reconstruction

Tibial tunnel placement during endoscopic anterior cruciate ligament (ACL) reconstruction has received increased emphasis in the recent literature. Appropriate tunnel length is a critical technical consideration. A tunnel that is too short results in graft extrusion, necessitating supplemental fixation techniques. A tunnel that is too long may make distal fixation and femoral tunnel placement difficult. A simple rule is proposed that allows for correct tunnel length and allows placement of the bone plug consistently within the tibial tunnel, allowing interference screw fixation.     

Pullout strength of tibial graft fixation in ACL-replacement with a patellar tendon graft: Interference screw versus staple fixation in human knees

Summary The endoscopic single incision technique for ACL reconstruction with a femoral half-tunnel may lead to a graft/tunnel mismatch and subsequent protrusion of the block from the tibial tunnel. The typical tibial fixation with an interference screw is not possible in these cases. Fixation with staples in a bony groove inferior to the tunnel outlet can be used as an alternative technique. Current literature does not provide biomechanical data of both fixation techniques in a human model. This study was performed to evaluate primary biomechanical parameters of this technique compared to a standard interference screw fixation of the block. 55 fresh-frozen human cadaver knee joints of a younger age (mean age: 44 years) were used. Grafts were harvested from the patellar tendon midportion with bone blocks of 25 mm length and 9 mm width. A 10 mm tibial tunnel was drilled from the anteromedial cortex to the center of the tibial insertion of the ACL. 3 different sizes of interference screws (7 × 30, 9 × 20 and 9 × 30 mm) were chosen as a standard control procedure (n = 40). For tibial bone-block fixation the graft was placed through the tunnel, the screw was then inserted on the cancellous or the cortical surface respectively. 15 knees were used for staple fixation. A groove was created inferior to the tunnel outlet with a chisel. The bone block was fixed in this groove with 2 barbed stainless steel staples. Tensile testing in both of the groups was carried out under axial load parallel to the tibial tunnel in a Zwick-testing-machine with a velocity of 1 mm/sec. Dislocation of the graft and stiffness were calculated at 175 N load. Maximum load to failure using interference screws varied between 506 and 758 N. Load to failure using staples was 588 N. Dislocation of the graft ranged between 3.6 and 4.7 mm for interference screw fixation and was 4.2 mm for staples. With both fixation techniques, the recorded failure loads were sufficient to withstand the graft loads which are to be expected during the rehabilitation period. Staple fixation of the bone block outside of the tunnel resulted in fixation strength comparable to interference screw fixation.      

Biomechanical properties of interference screw implantation in replacement of the anterior cruciate ligament with patellar and hamstring transplants. An experimental study using roentgen stereometry analysis (RSA)

This experimental roentgen stereometric analysis (RSA) study was performed to measure micromotions between the graft and tunnel under submaximal load in anterior cruciate ligament (ACL) reconstruction. The aim was to evaluate the maximum load at failure, linear stiffness, and slippage of bone-patellar-tendon-bone (BPTB) grafts fixed with interference screws compared to quadrupled hamstring grafts fixed with interference screws. We used 15 porcine tibia specimens for the study. In the BPTB group, the 10 x 25-mm bone plugs were fixed in a tunnel with 10 mm in diameter using a 7 x 25-mm titanium interference screw (n = 5) or a biodegradable screw (n = 5). The five hamstring transplants were folded to a four-stranded graft and armed with a baseball stitch suture. The sutured side was fixed with a 7 x 25-mm biodegradable polylactide screw in an 8-mm tunnel. The tibial bones, tendon grafts, and interference screws were marked with tantalum beads. The grafts were mounted to a custom made load frame and loaded parallel to the axis under RSA control increasing the force in steps of 50 N. Micromotions between bone plug, screw, and tibia were measured with RSA. Accuracy of RSA for the in vitro study was evaluated as 0.05 mm. Hamstring grafts failed at significantly lower maximum loads (492 +/- 30 N) than BPTB grafts (658 +/- 98 N). Linear stiffness of the hamstring graft fixations was eight times lower compared to the BPTB grafts (403 +/- 141 N/mm vs 3500 +/- 1300 N/mm). There was no significant difference between the biodegradable and titanium screws in the BPTB group. Slippage of the graft started at 82 +/- 35 N load in the hamstring group and at 428 +/- 135 N in the BPTB group. Slippage of the graft at 75% of the maximum pull-out strength was measured as 0.36 +/- 0.25 mm in the BPTB and 2.58 +/- 1.08 mm in the hamstring group. The interference screw fixation of a quadrupled hamstring graft showed a lower linear stiffness and an earlier slippage compared to a patellar tendon bone plug. Slippage of the hamstring grafts at submaximal loads may result in fixation failure during rehabilitation.     

Biomechanical evaluation of patellar- and hamstring tendon graft fixation for anterior cruciat ligament reconstruction using a poly-(D, L-lactide) interference screw

Anterior cruciate ligament (ACL) reconstruction using autologous hamstring tendons are being performed more frequently and satisfactory results have been reported. Advantages such as low donor site morbidity and ease of harvest as well as disadvantages like low initial construct stiffness have been described. Recently, it has been demonstrated that graft fixation close to the original ACL insertion sites increases anterior knee stability and graft isometry. Hamstring tendon fixation techniques using interference screws offer this possibility. To reduce the risk of graft laceration, a round threaded titanium interference screw (RCI) was developed. To improve initial fixation strength, fixation techniques for hamstring tendons with separate or attached tibial bone plugs were introduced. However, data on fixation strength do not yet exist. With respect to the proposed advantages of biodegradable implants, like undistorted magnetic resonance imaging, uncompromised revision surgery and a decreased potential of graft laceration during screw insertion, we performed pullout tests of round threaded biodegradable and round threaded titanium interference screw fixation of semitendinosus (ST) grafts with and without distally attached tibial bone plugs. Data were compared with bone-tendon-bone (BTB) graft fixation using biodegradable and conventional titanium interference screws. We used 56 proximal calf tibiae to compare maximum pullout force, screw insertion torque, and stiffness of fixation for biodegradable direct ST tendon and bone plug fixation (group I: without bone plug, group II: with bone plug) versus titanium interference screw fixation (group III: without bone plug, group IV: with bone plug). A round threaded biodegradable poly-(D, L-lactide) (Sysorb) and a round threaded titanium interference screw (RCI) were used. As a control calf bone-tendon-bone (BTB) grafts fixed with either poly-(D, L-lactide) (group V) or conventional titanium (group VI) interference screws were used. ST tendons were harvested either with or without their distally attached tibial bone plugs from human cadavers and were folded to a three-stranded graft. Specimen were loaded in a material testing machine with the applied load parallel to the long axis of the bone tunnel. Maximum pullout force of ST bone plug (group III: 717 N +/- 90, group IV: 602 N +/- 117) fixation was significantly higher than that of direct tendon (group I: 507 N +/- 93, group III: 419 N +/- 77) fixation. Maximum pullout force of biodegradable screw ST fixation was higher than that of titanium screw fixation in both settings. There was no significant difference in pullout force between biodegradable (713 N +/- 210) and titanium (822 N +/- 130) BTB graft fixation or between ST fixation with bone plug and biodegradable screw with BTB fixation. Pullout force of hamstring tendon interference screw fixation can be improved by using a biodegradable implant. In addition, initial pullout force can be greatly improved by harvesting the hamstring tendon graft with its distally attached tibial bone plug. This may be important, especially in improving tibial graft fixation. This study encourages further research in tendon-bone healing with direct interference screw fixation to confirm the potential of this advanced method.