Urea-doped carbon dots as fluorescent switches for the selective detection of iodide ions and their mechanistic study

A facile and green strategy for the fabrication of fluorescent urea-doped carbon dots (N-CDs) has been explored. Significantly, the fluorescent N-CDs could recognize iodide ions (I⁻) with high selectivity, and their photoluminescence could be efficiently quenched by the addition of I⁻. The sensitivity analysis for I⁻ indicated a linear relationship in the range from 12.5 to 587 μM with the detection limit as low as 0.47 μM. Furthermore, the I⁻ induced fluorescence (FL) quenching mechanism was investigated employing a combination of techniques, including UV-vis/fluorescence spectroscopy, Density Functional Theory (DFT) calculation, TEM and time-resolved fluorescence decay measurements. The DFT calculation results demonstrated that the amino- and amide groups of N-CDs play a significant role in iodide recognition through the formation of multiple N–H⋯I⁻, C–H⋯I⁻ and C( O)N–H⋯I⁻ interactions with I⁻. The TEM experiment confirmed the aggregation process when I⁻ was added to the N-CDs solution. Moreover, the radiative decay rate of N-CDs, which was first measured and reported the kinetic behaviors of the FL-quenching process, decreased from 3.30 × 10⁷ s⁻¹ to 1.95 × 10⁷ s⁻¹ after the coordination with I⁻ ions. The reduced lifetime demonstrated that the excited energy dissipation led to a dynamic quenching process. Therefore, such carbon materials can function as effective fluorescent switches for the selective detection of I⁻ ions.

Urea-doped carbon dots (N-CDs) have been successfully fabricated for monitoring iodide ions; the reduced lifetime of N-CDs demonstrated that the excited energy dissipation led to a dynamic fluorescence quenching process.     

A coumarin–dihydroperimidine dye as a fluorescent chemosensor for hypochlorite in 99% water

The hypochlorite anion (OCl⁻), a reactive oxygen species (ROS), is an important microbicidal agent in the immune system. Accurate and selective detection of OCl⁻ in environmental and biological samples by a fluorescent molecular sensor is an important subject. All previously reported sensors, however, have suffered from tedious multi-step synthesis for the sensors and the use of large amounts of organic solvents for the analysis. Herein, we report that a coumarin–dihydroperimidine dye prepared by facile condensation behaves as a fluorescent sensor for OCl⁻ in 99% water. The sensor exhibits weak fluorescence, but OCl⁻-selective dehydrogenation of its dihydroperimidine unit creates a strong blue fluorescence. This turn-on fluorescence response facilitates selective and sensitive detection of OCl⁻ in the physiological pH range. Ab initio calculation revealed that the fluorescence enhancement by OCl⁻ is triggered by intramolecular proton transfer from the coumarin –OH to the imine nitrogen of the formed perimidine moiety.

A coumarin–dihydroperimidine dye exhibits strong blue fluorescence by OCl⁻-selective dehydrogenation of the dihydroperimidine unit, and facilitates selective and sensitive fluorometric detection of OCl⁻ in 99% water.     

Novel N,Cl-doped deep eutectic solvents-based carbon dots as a selective fluorescent probe for determination of morphine in food

In the present study, new N,Cl co-doped carbon dots (N,Cl-CDs) based on deep eutectic solvent (DES) were fabricated by a facile hydrothermal process. This fluorescent probe exhibited a good quantum yield of 14% and was applied for the sensitive and selective quantification of morphine in foods. In addition, the influence of solution pH, interaction time, system temperature, interfering substances and analogues on the determination was also investigated. Under the optimized conditions, the luminescence intensity of carbon dots increased linearly with the addition of morphine in the concentration range of (0.15–280.25) μg mL⁻¹ (R² > 0.9969) and the limit of detection (LOD) of 46.5 ng mL⁻¹. Based on these results, it is suggested that N,Cl-CDs is a promising fluorescent probe for sensitive and selective quantification of morphine in foods.

A schematic illustrating the synthesis and morphine detection of N,Cl-CDs.     

Facile synthesis of N,P-doped carbon dots from maize starch via a solvothermal approach for the highly sensitive detection of Fe3+

Nitrogen/phosphorus-doped carbon dots (N, P-CDs) with a quantum yield as high as 76.5% were synthesized by carbonizing maize starch via a facile ethanol solvothermal approach. Transmission electron microscopy (TEM) measurement shows that the as-prepared N, P-CDs displayed a quasi-spherical shape with a mean size of ca. 2.5 nm. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy disclosed the presence of –OH, –NH₂, –COOH, and –CO functional groups over the surface of N, P-CDs. On the basis of excellent fluorescent properties with strong blue fluorescence emission at 445 nm upon excitation at 340 nm, these N, P-CDs were adopted as a fluorescent probe towards the effective detection of Fe³⁺ ions in water. The limit of detection (LOD) was as low as 0.1 μmol L⁻¹ and showed a better linear relationship in the range of 0.1 ∼ 50 μmol L⁻¹. In conclusion, these synthesized N, P-CDs can be efficiently used as a promising candidate for the detection of Fe³⁺ ions in some practical samples.

Nitrogen/phosphorus-doped carbon dots (N, P-CDs) with a quantum yield as high as 76.5% were synthesized by carbonizing maize starch via a facile ethanol solvothermal approach and utilized for the detection of Fe³⁺.     

Fluorescent sensing platform based on green luminescence carbon dots and AuNPs for clenbuterol detection in pork liver

In this paper, water-soluble green fluorescent carbon dots (G-CDs) were prepared using p-phenylenediamine and glutathione (GSH) as the precursors. The G-CDs exhibit excellent optical properties, and the maximum emission wavelength is located at 522 nm (under 410 nm excitation), which greatly overlaps with the absorption spectrum of AuNPs. Consequently, an effective “off–on” fluorescent sensing platform involved in G-CDs and AuNPs for detection of clenbuterol (CLB) was constructed. The fluorescence of G-CDs was strongly quenched by AuNPs due to the inner filter effect (IFE). As CLB was introduced, the quenched fluorescence intensity was recovered due to the specific interaction between the AuNPs and CLB. The recovered fluorescence intensity is linear to CLB concentration in the range of 13–270 ng mL⁻¹ with a low detection limit of 3.75 ng mL⁻¹. The prepared sensor has been successfully applied for CLB detection in pork liver and could be utilized in food analysis.

Carbon dots (G-CDs) with bright green fluorescence are synthesized by hydrothermal treatment of p-phenylenediamine and glutathione. Employing the G-CDs and AuNPs as sensing platform, a simple fluorescence sensor to detect clenbuterol was established.     

Early detection of bacteria using SPR imaging and event counting: experiments with Listeria monocytogenes and Listeria innocua

Foodborne pathogens are of significant concern in the agrifood industry and the development of associated rapid detection and identification methods are of major importance. This paper describes the novel use of resolution-optimized prism-based surface plasmon resonance imaging (RO-SPRI) and data processing for the detection of the foodborne pathogens Listeria monocytogenes and Listeria innocua. With an imaging spatial resolution on the order of individual bacteria (2.7 ± 0.5 μm × 7.9 ± 0.6 μm) over a field of view 1.5 mm², the RO-SPRI system enabled accurate counting of individual bacteria on the sensor surface. Using this system, we demonstrate the detection of two species of Listeria at an initial concentration of 2 × 10² CFU mL⁻¹ in less than 7 hours. The surface density of bacteria at the point of positive detection was 15 ± 4 bacteria per mm². Our approach offers great potential for the development of fast specific detection systems based on affinity monitoring.

A dedicated SPR apparatus optimized for individual bacteria observation and a new strategy for early detection of microorganisms in growth.     

Fluorescent probe based on N-doped carbon dots for the detection of intracellular pH and glutathione

Carbon dots (CDs) as fluorescent probes have been widely exploited to detect biomarkers, however, tedious surface modification of CDs is generally required to achieve a relatively good detection ability. Here, we synthesized N-doped carbon dots (N-CDs) from triethylenetetramine (TETA) and m-phenylenediamine (m-PD) using a one-step hydrothermal method. When the pH increases from 3 to 11, the fluorescence intensity of the N-CDs gradually decreases. Furthermore, it displays a linear response to the physiological pH range of 5–8. Au³⁺ is reduced by amino groups on the surface of N-CDs to generate gold nanoparticles (AuNPs), causing fluorescence quenching of the N-CDs. If glutathione (GSH) is then added, the fluorescence of the N-CDs is recovered. The fluorescence intensity of the N-CDs is linearly correlated with the GSH concentration in the range of 50–400 μM with a limit of detection (LOD) of 7.83 μM. The fluorescence probe was used to distinguish cancer cells from normal cells using pH and to evaluate intracellular GSH. This work expands the application of CDs in multicomponent detection and provides a facile fluorescent probe for the detection of intracellular pH and GSH.

N-doped carbon dots used as a fluorescence probe can distinguish cancer cells from normal cells by pH and evaluate intracellular GSH.     

A dual emission metal–organic framework for rapid ratiometric fluorescence detection of CO32− in seawater

A dual emission metal–organic framework (IRMOF-10-Eu) was prepared and used as a ratiometric fluorescent sensor for CO₃²⁻ detection. IRMOF-10-Eu had good stability and excellent luminescence in aqueous solution. IRMOF-10-Eu showed dual fluorescence emission from the ligand and Eu³⁺ with single excitation. Upon treatment with CO₃²⁻, the fluorescence ratio (I₆₂₄/I₃₅₈) of the probe displayed significant change. The relative fluorescence intensity ratio (I₆₂₄/I₃₅₈) and CO₃²⁻ concentration had a linear relationship in 50–300 μM range with a low detection limit of 9.58 μM. And the luminescence probe of CO₃²⁻ showed a fast detection time. The possible mechanism was investigated. CO₃²⁻ changed the structure of IRMOF-10-Eu and interrupted the energy transfer process. Thus, the fluorescence emission intensity of the ligand was increased and Eu³⁺ was decreased with the addition of CO₃²⁻. IRMOF-10-Eu was used to detect CO₃²⁻ in seawater, which showed good prospect in practical application. Subsequently, a highly selective and sensitive probe, IRMOF-10-Eu, may pave an efficient way for CO₃²⁻ detection in seawater.

A dual emission metal–organic framework (IRMOF-10-Eu) was prepared and used as a ratiometric fluorescent sensor for CO₃²⁻ detection.     

A sensitive and selective BINOL based ratiometric fluorescence sensor for the detection of cyanide ions

A highly selective, novel BINOL based sensor BBCN has been developed for the fluorescent ratiometric detection of cyanide ions (CN⁻). The optical study revealed that BBCN exhibited unique spectral changes only with cyanide ions in the presence of other competing ions. Besides, an apparent fluorescent colour change from green to blue was observed. A clear linear relationship was observed between the fluorescence ratiometric ratio of BBCN and the concentration of CN⁻ with a reasonably low detection limit (LOD) of 189 nM (507 ppb). The optical response was due to the nucleophilic addition of CN⁻ to the dicyanovinyl group of the sensor, which compromises the probe''s intramolecular charge transfer. This mechanism was well confirmed by Job''s plot, ¹H-NMR and ESI-MS studies. BBCN showed immediate spectral response towards (1 second) CN⁻ and detection could be realized in a broad pH window. Furthermore, the practical utility of BBCN was studied by test paper-based analysis and the detection of CN⁻ in various water resources.

A highly selective, novel BINOL based sensor BBCN has been developed for the fluorescent ratiometric detection of cyanide ions (CN⁻).     

Sensitive distance-based paper-based quantification of mercury ions using carbon nanodots and heating-based preconcentration

This article reports the first fluorescent distance-based paper device coupled with an evaporating preconcentration system for determining trace mercury ions (Hg²⁺) in water. The fluorescent nitrogen-doped carbon dots (NCDs) were synthesized by a one-step microwave method using citric acid and ethylenediamine. The fluorescence turn-off of the NCDs in the presence of Hg²⁺ was visualized with a common black light, and the distance of the quenched fluorescence correlated to Hg²⁺ concentration. The optimal conditions for pH, NCD concentration, sample volume, and reaction time were investigated. Heating preconcentration was used to improve the detection limits of the fluorescent distance-based paper device by a factor of 100. Under the optimal conditions, the naked eye limit of detection (LOD) was 5 μg L⁻¹ Hg²⁺. This LOD is sufficient for monitoring drinking water where the maximum allowable mercury level is 6 μg L⁻¹ as established by the World Health Organization (WHO). The fluorescent distance-based paper device was successfully applied for Hg²⁺ quantification in water samples without interference from other cations. The proposed method provides several advantages over atomic absorption spectroscopy including ease of use, inexpensive material and fabrication, and portability. In addition, the devices are simple to fabricate and have a long shelf-life (>5 months).

This article reports the first fluorescent distance-based paper device coupled with an evaporating preconcentration system for determining trace mercury ions (Hg²⁺) in water.     

Green synthetic nitrogen-doped graphene quantum dot fluorescent probe for the highly sensitive and selective detection of tetracycline in food samples

Tetracycline (TC) is a broad-spectrum antibiotic. When humans consume too much food containing tetracycline residues, it can be a serious health hazard. Therefore, it is essential to develop a strategy to detect TC. In this study, we prepared light blue-green luminescent nitrogen-doped graphene quantum dots (N-GQDs) by a hydrothermal method using the natural products potato straight-chain starch and urea as precursors; the fluorescence quantum yield of the prepared N-GQDs was 5.2%. We investigated the detection of tetracycline (TC) by this N-GQD fluorescent sensor based on the internal filtration effect (IFE) of TC on N-GQDs. The reaction is green, simple and no other contaminating products are present. A good linear relationship was established between the relative fluorescence intensity ratio of the system and the logarithm of the TC concentration of 2.5 × 10⁻¹⁰ to 5 × 10⁻⁶ M (R² = 0.9930), with a detection limit of 9.735 × 10⁻¹³ M. The method has been used to analyze TC in three real food samples (whole milk, skim milk, honey) with low detection limits (3.750 × 10⁻¹¹ to 2.075 × 10⁻⁹ M), wide linear range, and satisfactory recoveries of 93.80–109.20% were obtained. In conclusion, the proposed method is a green, rapid, highly sensitive and selective method for the detection of tetracycline in real food samples, demonstrating the potential application of N-GQDs in food detection.

Tetracycline (TC) is a broad-spectrum antibiotic.     

Highly photoluminescent and temperature-sensitive P, N,B-co-doped carbon quantum dots and their highly sensitive recognition for curcumin

Water-soluble P, N, B-co-doped carbon quantum dots (PNBCDs) synthesized using a convenient hydrothermal method exhibit many excellent features, such as strong fluorescence, excitation-independent emission, high monodispersity, good stability, and excellent water solubility with a fluorescence quantum yield of 21.95%. The as-prepared PNBCDs possessed remarkable selectivity and sensitivity towards curcumin with the linear range of 0–1.5 μmol L⁻¹ and the detection limit for curcumin was 68 nmol L⁻¹ (3σ/k). Additionally, the wonderfully reversible and repeatable sensitivity to external temperature makes it possible that the PNBCDs could be used as a biocompatible fluorescent ink and for thermo-sensitive devices.

Temperature-sensitive P, N, B-co-doped carbon quantum dots (PNBCDs) synthesized using one-pot method exhibit many excellent features, such as strong fluorescence, good stability and sensitive detection for curcumin.     

Development of a fluorescent immunochromatographic assay based on quantum dots for the detection of fleroxacin

Fleroxacin (FLE) is a broad-spectrum fluoroquinolone antibiotic widely used in animal husbandry, veterinary medicine and aquaculture. Eating animal-derived foods with FLE residues can cause allergies, poisoning or drug resistance. The water-soluble QDs (CdSe/ZnS) and anti-FLE monoclonal antibody (mAb) were used to prepare a fluorescent probe by the method of N-(3-dimethylaminopropyl)-N′-ethylcarbodimide hydrochloride (EDC) activation. The fluorescent probe was characterized by dynamic light scattering (DLS). The better bioactivity and stability of the fluorescent probe was obtained under the pH value of 8.0, the molecule molar ratio of EDC (1 : 2000) and anti-FLE monoclonal antibodies (1 : 10). The control line (C line) and test line (T line) of a nitrocellulose (NC) filter membrane were sprayed with SPA (0.05 mg mL⁻¹) and FLE-OVA (1.4 mg mL⁻¹) solutions with optimal concentration, respectively. A novel method of fluorescent immunochromatographic assay based on quantum dots (QDs-ICA) in this work exhibited good accuracy, reproductivity and excellent specificity under the optimal experimental conditions. Compared with the traditional method for the visual detection of FLE, the developed QDs-ICA can successfully determine FLE residues in pork meat with a better cut-off value of 2.5 ng mL⁻¹. The QDs-ICA could be adapted for the rapid preliminary detection of FLE residues in pork meat for the first time.

(1) Water-soluble CdSe/ZnS QDs and an anti-FLE monoclonal antibody were used to prepare a fluorescent probe. (2) Primary rapid detection of FLE residues with visual fluorescent detection method.     

Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

A convenient, fast, sensitive and highly selective fluorescence sensor for the detection of glutathione (GSH) based on DNA derived bio-dots (DNA bio-dots)/polydopamine (PDA) nanoparticles was constructed. The fluorescent switch of DNA bio-dots was induced to turn off because of fluorescence resonance energy transfer (FRET) reactions between DNA bio-dots and PDA. The presence of GSH blocked the spontaneous oxidative polymerization of dopamine (DA) to PDA, leading the fluorescent switch of DNA bio-dots to be “turned on”. The degree of fluorescence recovery of DNA bio-dots is linearly correlated with the concentration of GSH within the range of 1.00–100 μmol L⁻¹, and the limit of detection (LOD) is 0.31 μmol L⁻¹ (S/N = 3, n = 9). Furthermore, the fluorescence sensor was successfully used to quantify GSH in human urine and glutathione whitening power, indicating the fluorescence sensor has potential in the detection of human body fluids and pharmaceutical preparations.

The turn-on fluorescence signal mechanism for detection of GSH.     

“Turn on” room-temperature phosphorescent biosensors for detection of hyaluronic acid based on manganese-doped ZnS quantum dots

Biosensors based on excellent optical properties of quantum dots (QDs) nanohybrids are efficient for biological detection. In this work, a room-temperature phosphorescent (RTP) PDAD–Mn–ZnS QDs biosensor was constructed with poly(diallyldimethylammonium chloride) (PDAD) as the modifier of MPA-capped Mn–ZnS QDs, and used to detect hyaluronic acid (HA). The newly-added HA induced severe electrostatic interaction with PDAD–Mn–ZnS QDs, leading to the aggregation between PDAD–Mn–ZnS QDs and HA and thereby enhancing RTP. The enhancement of RTP was proportional to the HA concentrations within certain ranges. On this basis, a high-performance HA sensor was built and this sensor had a detection limit of 0.03 μg mL⁻¹ and a detection range of 0.08–2.8 μg mL⁻¹. This proposed RTP sensor can avoid interferences from the background fluorescence or scattering light of the matrix that are encountered in spectrofluorometry. Thus, this biosensor is potentially suitable for detection of HA in real samples without complicated pretreatment.

Fabricating PDAD–Mn–ZnS QDs nanohybrids as a facile room-temperature phosphorescent biosensor for detection of hyaluronic acid.     

Fabrication of biocompatible magneto-fluorescence nanoparticles as a platform for fluorescent sensor and magnetic hyperthermia applications

Multifunctional nanoparticles with special magnetic and optical properties have been attracting a great deal of attention due to their important applications in the bioanalytical and biomedical fields. In this study, we report the fabrication of biocompatible magneto-fluorescence nanoparticles consisting of carbon dots (CDots) and silica-coated cobalt–manganese nanoferrites (Co_0.5Mn_0.5Fe₂O₄) (CoMnF@Si@CDots) (MagSiCDots) by a facile hydrothermal method. The as-prepared MagSiCDots have a particle size of 100–120 nm and show a negative zeta potential of −35.50 mV at a neutral pH. The fluorescence spectrum of the MagSiCDots nanoparticles consists of sharp excitation at 365 nm and broad blue light emission with a maximum wavelength of 442.5 nm and the MagSiCDots exhibit superparamagnetic behaviour with a saturation magnetization of 11.6 emu g⁻¹. The potential of MagSiCDots as a fluorescent sensor and be used for magnetic hyperthermia applications. It is seen that the fluorescent intensity of a colloidal solution (a hydrogen sulfide (H₂S) solution containing MagSiCDots nanoparticles) has a linear relationship with the H₂S concentration range of 0.2–2 μM. The limit of detection (LOD) of H₂S by our MagSiCDots particles is 0.26 μM and they remain stable for at least 90 min. To test the suitability of the MagSiCDots nanoparticles for use in hyperthermia application, induction heating using an AMF was done. It was observed that these nanoparticles had a specific absorption rate (SAR) of 28.25 W g⁻¹. The in vitro and in vivo cytotoxicity of MagSiCDots were tested on HeLa cells lines. The results show a cell viability of about 85% when exposed to 100 μg mL⁻¹ concentration of the particles. The in vivo cytotoxicity using zebrafish assay also confirmed the non-toxicity and biocompatibility of the nanoparticles to living cells. The reported data demonstrate that by combining CoMnF@Si and fluorescent CDots into a single system, not only nontoxic multifunctional nanomaterials but also multimodal nanoparticles for several applications, such as hazard gas detection and acting as a biocompatible heat source for therapeutic treatment of cancer, are provided.

Multifunctional nanoparticles with special magnetic and optical properties have been attracting a great deal of attention due to their important applications in the bioanalytical and biomedical fields.     

One-step synthesis of fluorescent graphene quantum dots as an effective fluorescence probe for vanillin detection

This study proposes an easy bottom-up method for the synthesis of photoluminescent (PL) graphene quantum dots (GQDs) using citric acid as the carbon source. The obtained GQDs were characterized by high-resolution transmission electron microscopy (HRTEM), UV-vis absorption spectroscopy, fluorescence spectroscopy, and Fourier transform infrared spectroscopy (FT-IR). The synthesised GQDs have an average diameter of 4.76 ± 0.96 nm, with a lattice spacing of 0.24 nm. The GQDs exhibit excitation-independent PL emission. The surface of the GQDs has a variety of functional groups (hydroxyl, carboxyl, and ether groups etc.) to enhance its stability and water solubility. In this study, a fluorescent “on–off” sensor is developed for the selective detection of vanillin in chocolates using GQDs as a fluorescent probe. Under optimal conditions, fluorescence intensity of the GQDs has a good linear relationship with the vanillin concentration (0.0–2.1 × 10⁻⁵ mol L⁻¹), with a limit of detection of 2.5 × 10⁻⁸ mol L⁻¹. For detection in real samples, the percent recovery of vanillin and the relative standard deviation were 88.0–108.9% and 0.90–5.4%, respectively. Thus, this GQDs-based method has good accuracy and precision and can be used for vanillin detection in practical applications.

This study proposes an easy bottom-up method for the synthesis of photoluminescent (PL) graphene quantum dots (GQDs) using citric acid as the carbon source.     

Ratiometric fluorescent sensors for nitrite detection in the environment based on carbon dot/Rhodamine B systems

A novel carbon dot/Rhodamine B-based ratiometric fluorescent probe was developed for a highly sensitivity and selective detection of nitrite (NO₂⁻). The probe showed colour changes from blue to orange under ultraviolet light in response to NO₂⁻ with a detection limit as low as 67 nM in the range of 0 to 40 μM. A ratiometric fluorescent test paper was successfully prepared using the probe solution, which demonstrated its feasibility towards a rapid and semi-quantitative detection of NO₂⁻ in real samples.

A visual ratiometric fluorescent sensor based on blue carbon dot/Rhodamine B is used to selectively detect NO₂⁻ in the environment.     

The use of S2O82− and H2O2 as novel specific masking agents for highly selective “turn-on” fluorescent switching recognition of CN− and I− based on Hg2+–graphene quantum dots

In this study, we report that both CN⁻ and I⁻ can enhance the fluorescent intensity of Hg²⁺–graphene quantum dots (Hg²⁺–GQDs). However, the selectivity of the sensor was poor. Accordingly, simple specific masking agents can be directly used to solve this problem. Here, for the first time, we report the use of persulfate ion (S₂O₈²⁻) as a turn-on fluorescent probe of Hg²⁺–GQDs for selective CN⁻ detection, while hydrogen peroxide (H₂O₂) was selected for its sensing ability towards I⁻ ion detection. Interestingly, the signal was immediately measured after addition of the masking agent to Hg²⁺–GQDs and the sample because its interaction was very fast and efficient. The method had a linear response in the concentration ranges of 0.5–8 μM (R² = 0.9994) and 1–12 μM (R² = 0.9998) with detection limits of 0.17 and 0.20 μM for CN⁻ and I⁻, respectively. The sensor was successfully used for the dual detection of both CN⁻ and I⁻ in real water samples with satisfactory results. In conclusion, the specific masking agents in a Hg²⁺–GQDs system appeared to be good candidates for fluorometric “turn-on” sensors for CN⁻ and I⁻ with excellent selectivity over other ions.

In this study, we report that both CN⁻ and I⁻ can enhance the fluorescent intensity of Hg²⁺–graphene quantum dots (Hg²⁺–GQDs).     

An innovative study on the “on–off–on” detection of sulfur ions based on a TSPP–riboflavin fluorescent probe

In this paper, 5,10,15,20-(4-sulphonatophenyl) porphyrin (TSPP) was synthesized by a facile route and used as a fluorescent probe to construct a sensor system based on the high water solubility and high quantum yield. It was found that when riboflavin (RF) was introduced into the TSPP solution, the fluorescence intensity of TSPP decreased for the peaks at 645 nm and 700 nm based on the principle of the electrostatic attractions and hydrophobic interactions between TSPP and riboflavin. When the fluorescence emission peak of riboflavin appeared at 550 nm, the fluorescence sensor system changed from the “on” state to the “off” state. When sulfur ions (S²⁻) were further introduced into the TSPP–riboflavin system, the fluorescence intensity of riboflavin was further decreased based on the specific reaction between S²⁻ and riboflavin. However, the fluorescence signal of TSPP was restored and the fluorescence sensing system changed from the “off” state to the “on” state. Therefore, TSPP was used as a fluorescent probe to construct an “on–off–on” fluorescent sensing system, the linear range of S²⁻ detected by this system is 5.0 × 10⁻⁹ to 3.6 × 10⁻⁵ M, and the detection limit (LOD) is 1.1 × 10⁻⁹ M. The sensing system has higher accuracy and sensitivity, and it can be successfully used in the sensing of S²⁻ in real samples.

In this paper, 5,10,15,20-(4-sulphonatophenyl) porphyrin (TSPP) was synthesized by a facile route and used as a fluorescent probe to construct a sensor system based on the high water solubility and high quantum yield.