HLA-DQA1和HLA-DQB1等位基因与皖籍汉族人群白癜风的相关性

目的 探讨HLA-DQA1、-DQB1等位基因与皖籍汉族人群白癜风的相关性。方法 采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,检测白癜风患者的HLA-DQA1、-DQB1等位基因。结果 与正常人对照组比较,①白癜风患者HLA-DQA1*0302、-DQB1*0303、-DQB1*0503等位基因频率显著升高,HLA-DQA1*0501等位基因频率显著降低;②HLA-DQA1*0302、-DQA1*0601、-DQB1*0303、-DQB1*0503等位基因频率在儿童型白癜风患者中显著升高,HLA-DQA1*0501等位基因频率显著下降;而成人型白癜风患者HLA-DQB10303等位基因频率显著升高;③HLA-DQA1*0302、-DQB1*0303、-DQB1*0503等位基因频率在泛发型白癜风患者中显著升高,HLA-DQA1*0501等位基因频率显著下降;而局限型白癜风患者HLA-DQB1*0303等位基因显著升高。结论 HLA-DQA1*0302、-DQA1*0601、-DQB1*0303、-DQB1*0503、-DQA1*0501等位基因可能与白癜风相关,不同类型白癜风在其遗传背景上可能存在异质性。     

色素障碍性皮肤病

20053290HLA-DQA1和HLA-DQB1等位基因与皖籍汉族人群白癜风的相关性/汪继之(安徽省皮防所),杨森,王红艳…∥中华皮肤科杂志.-2005,38(6).-357~359采用聚合酶链反应-序列特异性引物(PCR-SSP)方法检测187例皖籍汉族白癜风患者及273例正常对照的HLA-DQA1,-DQB1等位基因。结果:HLA-DQA1*0302,-DQB1*0303,-DQBP*0503等位基因频率在白癜风患者中显著升高;在儿童型患者中尚有HLA-DQA1*0601等位基因频率显著升高。白癜风组HLA-DQA1*0501等位基因频率显著降低。提示:HLA-DQA1*0302,-DQA1*0601,-DQB1*0303,-DQB1*0503,-DQA1*0501等位基因可能与白癜风相关,不同类型白癜风在其遗传背景上可能存在异质性。表1参6(穆欣)20053291白癜风患者血清可溶性细胞间黏附分子-1的检测/周渭珩(杭州市三院),洪为松,许爱娥∥中国中西医结合皮肤性病学杂志.-2005,4(1).-20~21采用酶联免疫吸附试验法检测40例白癜风患者血清中SICAM-1水平。结果:两组比较,白癜风患者...     

HLA-DQA1及DQB1等位基因与寻常型银屑病遗传易感性研究

目的 探讨HLA-DQA1和DQB1等位基因与汉族人寻常型银屑病遗传易感性。方法 利用聚合酶链反应-序列特异引物(PCR-SSP)法,对189例银屑病患者和273例健康人的HLA-DQA1和DQB1等位基因进行检测。结果 ①HLA-DQA1*0104和DQA1*0201与汉族人银屑病呈正相关性(Pc<0.05);DQA1*0501与汉族人银屑病呈负相关(Pc<0.001).②HLA-DQA1*0104、DQA1*0201和DQA1*0501等位基因与Ⅰ型银屑病发病有关。③HLA-DQA1*0104和DQA1*0201等位基因在有家族史和无家族史患者中的频率显着性增高。HLA-DQA1*0501仅在无家族史银屑病患者中显着性下降。结论 ①HLA-DQA1*0104和DQA1*0201可能是银屑病的易感基因或与易感基因相连锁;DQA1*0501等位基因可能具有阻止汉族人发生银屑病的作用。②有家族史和无家族史银屑病患者在其遗传背景上可能存在差异。     

山东汉族梅毒患者与HLA-A、B等位基因的相关性研究

目的 探讨山东汉族梅毒患者与HLA-A、B等位基因的相关性.方法 采用PCR-序列特异性寡核苷酸探针杂交(PCR-SSOP)方法对205例山东汉族梅毒患者与5844例山东汉族正常对照的HLA-A、B等位基因表现频率进行检测.结果 梅毒患者组HLA-A*02,B*15、40等位基因频率高于对照组(P值均＜0.01;Pc值均＜0.05),HLA-A*26等位基因频率低于对照组(P=0.003;Pc=0.039),HLA-B*15、40等位基因频率在显性梅毒组高于对照组(P值均＜0.01;Pc值均＜0.05),HLA-A*02、11、29,B*15、40等位基因频率在隐性梅毒组高于对照组(P值均＜0.01;Pc值均＜0.05),HLA-A*30、33等位基因频率在隐性梅毒组低于对照组(P值、Pc值分别为0.002、0.026;0.001、0.013),HLA-A*30等位基因频率在显性梅毒组高于隐性梅毒组(P=0.001;Pc=0.013).结论 HLA-A*02,B*15、40等位基因可能与山东汉族梅毒相关,HLA-A*30可能与山东汉族显性梅毒相关,HLA-A*02、11、29可能与山东汉族隐性梅毒相关.

Abstract：

Objective To investigate the association of HLA-A and -B alleles with syphilis in Shandong Han population. Methods The allele frequencies of HLA-A and -B were detected in 205 patients with syphilis and 5844 normal human controls by PCR-sequence specific oligonucleotide probe (PCR-SSOP)method. Results The patients with syphilis showed a higher frequency of HLA-A*02, B*15, B*40 alleles(all P＜0.01, Pc＜0.05) and a lower frequency of HLA-A*26 allele (P= 0.003, Pc = 0.039) than the normal human controls did. There was an increased frequency of HLA-B*15 and B*40 alleles in patients with symptomatic syphilis (both P＜0.01, Pc＜0.05), as well as an elevated frequency of HLA-A*02, 11, 29, B*15 and 40 alleles (all P＜0.01, Pc＜0.05) and a decreased frequency of HLA-A*30 and 33 in patients with asymptomatic syphilis(P=0.002, 0.026, Pc=0.001, 0.013 respectively), compared with the normal human controls. The frequency of HLA-A*30 allele was significantly higher in patients with symptomatic syphilis than in those with asymptomatic syphilis (P = 0.001, Pc = 0.013). Conclusions There seems to be an association between HLA-A*02, B* 15 and B*40 alleles and syphilis, between HLA-A*30 allele and symptomic syphilis, and between HLA-A*02, 11 and 29 alleles and asymptom1atic syphilis, in Shandong Han population.     

HLA-DQ/DP等位基因与粤籍汉族斑秃及中医证型的相关性研究

目的探讨HLA-DQ/DP等位基因与粤籍汉族斑秃及中医证型的相关性。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)分型技术,对51例粤籍汉族斑秃患者的HLA-DQ/DP等位基因进行检测,并与110名粤籍汉族健康人群进行对照。结果 HLA-DQA1*0201、DQA1*0601、DQB1*0501、DQB1*0602、DPA1*0103基因频率斑秃组显著高于对照组,有极显著性差异(P0.05或P0.01);DQB1*0301、DPA1*0201基因频率斑秃组显著低于对照组(P0.05);DQA1*0301气血两虚证基因频率显著高于其他证型(P0.05)。结论 HLA-DQA1*0201、DQA1*0601、DQB1*0501、DQB1*0602、DPA1*0103可能是斑秃的易感基因,DQB1*0301、DPA1*0201可能是斑秃的保护基因,DQB1*0301主要与重型组有关,DQB1*0501、DPA1*0103则与轻型组相关,DQA1*0301可能是气血两虚证的易感基础。     

广东汉族白癜风与HLA-Ⅱ类基因相关性研究

目的:确定广东籍汉族白癜风发病与HLA-Ⅱ类基因的相关性。方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)技术,对57例广东籍汉族各型白癜风患者和60例健康对照者静脉血样本HLA-DR,DQ等位基因多态性进行研究。结果:白癜风患者DR7(DRB1*0701)等位基因频率显著升高(RR=6.213,Pc0.05),DRw52(DRB3*0101/02 DRB3*0201 DRB3*0301),DRw53(DRB4*0101/03/05)和DRw51(DRB5*0101/02 DRB5*0202)基因频率明显低于正常对照组,以上三者两组间比较均有显著性差异(Pc0.05)。白癜风患者DQ5(DQB1*0501-04)基因频率显著高于正常对照组(Pc0.05);DQ4(DQB1*0401/02)基因频率显著低于正常对照组(Pc0.05)。结论:提示HLA-DR7(DRB1*0701)等位基因可能与广东籍汉族白癜风的发病有关。DRw52(DRB3*0101/02 DRB3*0201 DRB3*0301),DRw53(DRB4*0101/03/05)和DRw51(DRB5*0101/02 DRB5*0202)对白癜风发病可能有一定保护作用。DQ5(DQB1*0501-04)可能为白癜风患者的易感基因。     

北方汉族寻常型银屑病与HLA等位基因的关联研究

目的:研究中国北方汉族寻常型银屑病(PsV)与HLA等位基因的关联性。方法:采用序列特异性引物-聚合酶链反应(PCR-SSP)方法检测91例PsV患者和102例健康人HLA-A、B、Cw、DRB1及DQB1等位基因。结果:(1)PsV患者HLA-A*0101-03、A*3001-04、B*5701、Cw*0602、Cw*0603/04/05、DRB1*0701/02及DQB1*0201基因频率较正常对照显著增高;Cw*0401基因频率显著下降(Pc<0.05)。(2)I型PsV患者HLA-A*0101-03、A*3001-04、B*5701、Cw*0602、DRB1*0701/02及DQB1*0201基因频率显著增高,而B*51、Cw*0401、DQB1*0301基因频率显著下降;Cw*0603/04/05基因频率在I型及II型PsV患者均显著增高(Pc<0.05)。有家族史PsV患者HLA-A*3001-04、DRB1*0701/02及DQB1*0201基因频率显著增高;无家族史患者Cw*0602基因频率显著增高,而DQB1*0501-04基因频率显著下降(Pc<0.05)。(3)HLA-A*3001-04、DRB1*0701/02及DQB1*0201基因频率仅在男性PsV患者显著增高;B*5701、Cw*0602基因频率仅在女性患者显著增高(Pc<0.05)。结论:(1)HLA-A*0101-03、A*3001-04、B*5701、Cw*0602、Cw*0603/04/05、DRB1*0701/02及DQB1*0201基因可能是北方汉族PsV的易感基因或与易感基因相连锁。(2)HLA-Cw*0401基因可能是阻止北方汉族PsV发病的“保护因子”。(3)I型或II型、有或无家族史PsV在遗传背景上存在差异。     

山东地区汉族大疱性类天疱疮与HLA-DR,DQB1基因的相关性研究

目的探讨HLA-DR,DQB1位点基因在大疱性类天疱疮(BP)易感性中的作用。方法用序列特异性引物-聚合酶链反应(PCR-SSP)方法,对49例BP患者及70例正常对照者进行了HLA-DR,DQB1等位基因的分型,并分析了上述基因在两组中的分布。结果与正常对照组比较,BP患者组DRB1*10基因频率明显增高(校正P值<0.05);DRB1*04-DQB1*0302连锁体频率、DRB1*10-DQB1*0501连锁体频率在BP组均显著高于对照组;DRB1*04在黏膜损害及大剂量皮质类固醇激素用量组显著增高。结论HLA-DR10(DRB1*10)可能是中国汉族BP的易感基因。DRB1*04-DQB1*0302连锁体、DRB1*10-DQB1*0501连锁体可能为汉族BP的易感连锁体。     

内蒙古汉族大疱性类天疱疮与HLA-DRB1和DQB1基因相关性分析

目的探讨内蒙古汉族大疱性类天疱疮与HLA-DRB1和DQB1基因相关性。方法采用聚合酶链反应-序列特异性引物技术(PCR-SSP),检测内蒙古汉族大疱性类天疱疮患者及内蒙古汉族正常人HLADRB1和DQB1基因分型,并统计分析。结果 HLA-DQB1*0301等位基因在大疱性类天疱疮患者组出现频率显著高于对照组(Pc0.05),HLA-DRB1*16和DQB1*0501等位基因在大疱性类天疱疮患者中出现频率显著低于对照组(Pc0.05)。结论 HLA-DQB1*0301可能是内蒙古汉族大疱性类天疱疮患者的遗传易感基因,而HLA-DRB1*16和DQB1*0501可能是内蒙古汉族大疱性类天疱疮患者的保护基因。     

广东汉族SLE患者HLA-DR、DQ、DP基因多态性研究

目的：研究广东汉族SLE患与HJA-DQ、DR、DP的相关性。方法：采用聚合酶链反应-序列特异性引物(PCR-SSP)技术，对48例广东籍汉族SLE患和102例健康对照静脉血样本HJA-DQ、DR、DP等位基因多态性进行研究。结果：SLE患DQA1*0101等位基因频率显升高(RR=8．12，P=0．004)，DQA1*0302明显低于正常组(RR=0．09，P=0．005)。DQB1*0301基因频率明显低于正常组，两比较有显性差异(P＜0．01)。SLE患DR3(DRB1*0301-DRB1*0302)基因频率显高于正常组(x。=14．24，P＜0．01，RR=20．20)；DRw52(DRB3*0101-DRB3*0301)基因频率显高于正常组(x^2=20．346，P＜0．01)；DRWl4：DRBl*1402，DRB1*1403基因频率也显高于正常组(P＜0．05)；DR4(DRB1*0401-DRB1*0411)基因频率明显低于正常组(P＜0．01)，DR9(DRB1*0901)，DRw11(DRB1*1101-DRB1*1104)基因频率也显低于正常组(P＜0．01)，DPA1*0202等位基因频率显高于正常组(x^2=4．124，P＜0．05，RR=3．54)，SLE患DPA1*0201等位基因频率显低于正常组(x^2=4．595，P＜0．05，RR=0．37)。结论：提示HLA-DQA1*0302、DQB1*0301；DR4(DRB1*0401-DRB1*0411)，DR9(DPB1*0901)，DRw11(DRB1*1101-DRB1*1104)对SLE发病可能有一定保护作用。DPA1*0202为广东籍48例SLE患的易感基因，而DPA1*0201可能为其保护基因。     

广西壮族系统性红斑狼疮与HLA-DQA1基因相关性研究

目的　为了探讨广西壮族系统性红斑狼疮 (SLE)与HLA DQA1相关性。方法　用聚合酶链反应 序列特异性引物 (PCR SSP)技术 ,对 5 1例SLE壮族患者和 70例壮族健康人的HLA DQA1基因进行研究。结果　两组均未发现HLA DQA1 0 2 0 1, 0 3 0 2及壮族健康人的DQA1 0 60 1等位基因。SLE组DQA1 0 10 1频率显著高于对照组 (RR =3 .2 72 7,χ2 =7.3 2 1,P =0 .0 0 9) ,而DQA1 0 10 4, 0 3 0 1频率均显著低于对照组 (RR =0 .45 61,χ2 =3 .885 ,P =0 .0 49和RR =0 .43 17,χ2 =4.843 ,P =0 .0 2 8)。结论　DQA1 0 10 1可能是广西壮族SLE易感基因 ,DQA1 0 10 4和DQA1 0 3 0 1可能为保护基因。     

广西地区汉族婴幼儿脉管性疾病与HLA-DRB1等位基因相关性研究

【摘要】 目的 探讨广西地区汉族婴幼儿脉管性疾病与HLA-DRB1等位基因遗传易感性的关系。 方法 广西地区汉族婴幼儿脉管性疾病145例（血管瘤组99例、脉管畸形组46例）,健康对照组105例。采用聚合酶链反应-序列特异性引物（PCR-SSP）方法对3组HLA-DRB1等位基因进行分型,使用SPSS16.0统计软件分析DRB1基因在3个组中的分布。 结果 DRB1*0901、*1401、*16等位基因在血管瘤组、脉管畸形组、对照组的分布差异均有统计学意义（χ2 = 13.05,P < 0.01;χ2 = 12.79,P < 0.01;χ2 = 10.36,P < 0.01）。进一步在各组间进行两两比较,DRB1*0901等位基因频率在血管瘤组与脉管畸形组间（RR = 4.84,P < 0.01）及血管瘤组与对照组组间（RR = 3.21,P < 0.01）差异均有统计学意义。DRB1*16等位基因频率在血管瘤组与对照组组间（RR = 2.25,P < 0.01）及脉管畸形组与对照组间（RR = 2.60,P < 0.01）差异均有统计学意义。血管瘤组中DRB1*1401等位基因频率显著性低于对照组（RR = 0.30,P < 0.01）。 结论 DRB1*0901等位基因可能为广西地区汉族婴幼儿血管瘤的易感基因,而DRB1*1401等位基因可能是其拮抗基因。HLA-DRB1*16等位基因可能为广西地区汉族脉管性疾病的易感基因。     

壮族人寻常性银屑病与HLA-DQA1和DQB1基因的相关性研究

[摘要]目的：探讨广西壮族人寻常型银屑病的发病与HLA-DQA1和DQB1基因的关联。方法：应用聚合酶链式反应-序列特异引物（PCR-SSP）法对58例壮族寻常型银屑病患者和102例健康壮族人的HLA-DQA1和DQB1座位进行基因分型，比较两组相应等位基因的频率。结果：HLA-DQB1*0303与壮族银屑病患者呈显著的正相关（OR=4.540，p=0.004），而HLA-DQA1*0501和HLA-DQB1*0301与壮族银屑病患者呈显著的负相关（OR=0.189，p=0.000；OR=0.367，p=0.018）。结论：以上3个HLA-DQ等位基因与广西壮族人寻常型银屑病的关系密切，其中HLA-DQB1*0303可能为该人群银屑病的易感因子，而HLA-DQA1*0501和HLA-DQB1*0301则可能对银屑病有抵抗作用。     

自体血清皮肤试验阳性慢性荨麻疹与HLA-DRB1基因的相关性研究

目的 探讨广西地区自体血清皮肤试验阳性慢性荨麻疹与HLA-DRB1等位基因遗传易感性的关系。 方法 对144例广西地区慢性荨麻疹患者进行自体血清皮肤试验,按试验结果分为阳性组62例,阴性组82例。采用聚合酶链反应-序列特异性引物方法,对患者组和199例正常人对照组进行HLA-DRB1等位基因的分型,并分析DRB1基因在3个组中的分布。使用SPSS13.0统计软件分析。结果DRB1*01、*1401、*16等位基因频率在阳性组、阴性组和正常人对照组间比较,差异均有统计学意义（χ2 = 10.92,Pc = 0.03;χ2 = 35.34,Pc < 0.01;χ2 = 12.69,Pc = 0.03）。进一步在各组间进行两两比较,仅DRB1*1401等位基因频率在自体血清皮肤试验阳性组与对照组间（RR = 17.09,Pc < 0.01）及自体血清皮肤试验阳性组与阴性组间（RR = 7.20,Pc < 0.01）,差异均有统计学意义。结论 DRB1*1401等位基因可能是广西地区自体血清皮肤试验阳性慢性荨麻疹的易感基因或与其连锁。     

Association of HLA-DQA1 and DQB1 genes with vitiligo in Chinese Hans

BACKGROUND: Vitiligo is an acquired depigmentary disorder of the skin and hair which results from selective destruction of melanocytes. Serological typing and genotyping of human leukocyte antigen (HLA) have shown discrepancies in HLA associations with vitiligo in different ethnic populations. METHODS: Polymerase chain reaction sequence-specific primer (PCR-SSP) method was used to analyze the distribution of HLA-DQA(1) and -DQB(1) alleles among 187 patients with vitiligo and 273 healthy controls through Epi Info version 6 package (Centers for Disease Control and Prevention, Atlanta, GA, USA). RESULTS: The frequencies of HLA-DQA1*0302 (OR = 1.98, P(c) < 0.01), -DQB1*0303 (OR = 3.14, P(c) < 0.001), and -DQB1*0503 (OR = 3.36, P(c) < 0.05) alleles were significantly increased in patients with vitiligo compared with controls, and HLA-DQA(1)*0501 (OR = 0.40, P(c) < 0.01) allele frequency was highly decreased. HLA-DQA1*0302 (OR = 5.19, P(c) < 0.001), -DQA1*0601 (OR = 2.95, P(c) < 0.05), -DQB1*0303 (OR = 4.50, P(c) < 0.001), and -DQB1*0503 (OR = 6.69, P(c) < 0.001) alleles were positively associated, whereas HLA-DQA1*0501 (OR = 0.05, P(c) < 0.001) allele was negatively associated with childhood vitiligo patients, and HLA-DQB1*0303 (OR = 2.76, P(c) < 0.001) allele was positively associated with adult vitiligo patients compared with controls. The frequency of HLA-DQB1*0303 (OR = 3.72, P(c) < 0.001) allele was significantly increased in localized vitiligo patients vs. controls, whereas HLA-DQA1*0302 (OR = 2.47, P(c) < 0.01), -DQB1*0303 (OR = 2.67, P(c) < 0.01), and -DQB1*0503 (OR = 4.46, P(c) < 0.01) allele frequencies were significantly increased and -DQA1*0501 (OR = 0.27, P(c) < 0.01) allele frequency was highly decreased in generalized vitiligo patients. CONCLUSIONS: HLA-DQA1*0302, -DQA1*0601, -DQB1*0303, and -DQB1*0503 alleles could be susceptible alleles of vitiligo, while HLA-DQA1*0501 allele could be a protective allele in Chinese Hans. There may be different genetic backgrounds between vitiligo patients of childhood and adult, localized and generalized.     

HLA-DQA1 and DQB1 alleles are associated with genetic susceptibility to psoriasis vulgaris in Chinese Han

BACKGROUND: Psoriasis vulgaris is a chronic skin disorder characterized by infiltration of inflammatory elements, keratinocyte hyperproliferation and altered differentiation. Although the pathogenesis of psoriasis is not fully understood, there is solid evidence of a susceptibility locus in the human leukocyte antigen (HLA) region. OBJECTIVES: To investigate whether HLA-DQA1 and DQB1 alleles are associated with genetic susceptibility to psoriasis vulgaris in Chinese Han. PATIENTS AND METHODS: The polymerase chain reaction-sequence-specific primer (PCR-SSP) method was used to analyse the distribution of HLA-DQA1 and DQB1 alleles in 189 patients with psoriasis and 273 healthy controls. RESULTS: The HLA-DQA1*0104 (OR = 2.33, P = 0.0001154, Pc = 2.0 x 10-3), DQA1*0201 (OR = 3.36, P < 1.0 x 10-7, Pc < 1.0 x 10-6), DQB1*0201 (OR = 1.64, P = 0.0192, Pc > 0.05) and DQB1*0303 (OR = 1.55, P = 0.0377, Pc > 0.05) alleles were more prevalent in patients with psoriasis vulgaris than in controls, and HLA-DQA1*0501 (OR = 0.30, P = 0.0000039, Pc < 4.0 x 10-5) alleles were less prevalent. The HLA-DQA1*0104 (OR = 2.42, P = 0.0001159, Pc < 2.0 x 10-3), DQA1*0201 (OR = 3.74, P < 1.0 x 10-7, Pc < 1.0 x 10-6) and DQA1*0501 (OR = 0.30, P = 0.0000374, Pc < 4.0 x 10-4) alleles were only associated with type I psoriasis. HLA-DQA1*0104 and DQA1*0201 were more prevalent in patients with or without a family history of psoriasis. However, the DQA1*0501 allele was only more prevalent in patients without a family history of psoriasis. CONCLUSION: HLA-DQA1*0104 and DQA1*0201 alleles may be psoriasis susceptibility genes or may be in close linkage with the susceptibility genes. The HLA-DQA1*0501 allele seems to have a protective effect against the development of psoriasis vulgaris in Chinese Han. There may be a difference in genetic background between psoriasis patients with and without a family history of psoriasis.     

新疆维吾尔族系统性红斑狼疮与HLA-DQA1等位基因相关性研究

目的 探讨新疆维吾尔族系统性红斑狼疮（SLE）与HLA-DQA1的相关性。 方法 用聚合酶链反应-序列特异性引物技术,对56例SLE维吾尔族患者和54例维吾尔族健康对照者的HLA-DQA1基因进行研究。 结果 SLE组DQA1* 0302频率显著高于对照组（χ2 = 10.032,P = 0.004）,而SLE组DQA1* 0101频率显著低于对照组（χ2 = 5.676,P = 0.017）。 结论 HLA-DQA1* 0302可能是新疆维吾尔族SLE的易感基因,HLA-DQA1* 0101可能是新疆维吾尔族SLE的保护基因。     

广西壮族系统性红斑狼疮与HLA-DRB1等位基因相关性研究

目的 探讨广西壮族系统性红斑狼疮(SLE)与HLA-DRB1基因的相关性。方法 用聚合酶链反应-序列特异性引物(PCR-SSP)方法,对52例SLE壮族患者和70例壮族健康人的HLA-DRB1基因进行研究。结果 SLE患者HLA-DRB1*1401及DRB1*16两个等位基因的频率低于正常对照组(RR=0.28,χ²=5.00,P=0.02及RR=0.39,χ²=3.95,P=0.05),患者组和对照组均未检出HLA-DRB1*08、DRB1*11和DRB1*13等位基因。结论 提示HLA-DRB1*1401及DRB1*16等位基因可能是广西壮族人SLE的保护基因,未发现易感基因。     

HLA-DQA1*0302、DQB1*0303与新疆维吾尔族白癜风的相关性研究

目的 探讨HLA-DQA1、DQB1等位基因与新疆维吾尔族白癜风相关性。方法 聚合酶链反应-序列特异性引物（PCR-SSP）检测300例维吾尔族白癜风患者HLA-DQA1*0302、DQB1*0303等位基因。结果 与300例维吾尔族正常人对照组相比,①白癜风患者DQA1*0302（20.5%比13.83%）、DQB1*0303（30.17%比13.33%）等位基因频率显著增高（P ＜ 0.01）;②HLA-DQA1*0302、DQB1*0303等位基因频率在成人型（发病年龄 ＞ 12岁）及儿童型（发病年龄≤12岁）的白癜风患者中均增高（P ＜ 0.01）;③HLA-DQB1*0303等位基因频率在有、无家族史的白癜风患者中均增高（P ＜ 0.01）,HLA -DQA1*0302等位基因频率在无家族史病例中显著增高（P ＜ 0.01）;④白癜风组儿童型和成人型两组间比较及有、无家族史两组间比较,DQA1*0302、DQB1*0303等位基因频率差异无统计学意义（P > 0.05）。 结论 HLA-DQA1*0302、DQB1*0303等位基因可能与新疆维吾尔族白癜风相关,儿童型和成人型及有、无家族史的白癜风在其遗传背景上可能存在异质性。