The association of the FOXC2-512C ＞ T gene polymorphism and metabolic syndrome in Mongol ethnic group

Objective To analyze the genetic mechanism and susceptibility of metabolic syndrome (MS) in the Mongolian in order to provide evidence for the early prevention, diagnosis and treatment of MS. Methods We used PCR-SSP to detect FOXC2-512C ＞ T gene polymorphism to compare the difference of frequency of the genotype and allele between the MS group and the control group. The relationships between FOXC2-512C ＞ T gene polymorphism and MS were analyzed through comparison of BMI, WHR, BP, TG, glucose and HDL among different genotypes. Results Significant larger or higher abdominal circumference, hip, BMI, glucose, TG, TC, SBP and DBP but lower HDL were observed in the MS group compared to those in the control group(P ＜0.05). FOXC2-512C ＞T gene distribution in the MS and control group conformed to Hardy-Weinberg equilibrium (x2 = 1.21, P =0.28 ;x2= 1.51 ,P =0.20 respectively). In the MS group, the frequency of C/C, T/C and T/T in the FOXC2-512C ＞ T gene was 26.7%, 58.3% and 15.0% respectively. However, the frequency in the control group was 13.9%, 39.3% and 46.7% respectively( P ＜0.05). The frequency of C and T allele between the MS and control group were significantly different( P ＜0.05 ). Multivariable Logistic regression results showed that FOXC2-512C ＞ T genotype,FBG, TG, BP and BMI were risk factors of MS in the Mongolian. Conclusions The study shows that T allele might be the protective factor of MS while C allele might be the risk factor. FOXC2-512C ＞T genotype is a highly risk factor of MS, which might be the new target of the therapy for MS. However, further larger study is warranted to verify whether FOXC2-512C ＞ T gene can be molecular genetic marker of Inner Mongolia in China.     

The association of the FOXC2-512C ＞ T gene polymorphism and metabolic syndrome in Mongol ethnic group

Objective To analyze the genetic mechanism and susceptibility of metabolic syndrome (MS) in the Mongolian in order to provide evidence for the early prevention, diagnosis and treatment of MS. Methods We used PCR-SSP to detect FOXC2-512C ＞ T gene polymorphism to compare the difference of frequency of the genotype and allele between the MS group and the control group. The relationships between FOXC2-512C ＞ T gene polymorphism and MS were analyzed through comparison of BMI, WHR, BP, TG, glucose and HDL among different genotypes. Results Significant larger or higher abdominal circumference, hip, BMI, glucose, TG, TC, SBP and DBP but lower HDL were observed in the MS group compared to those in the control group(P ＜0.05). FOXC2-512C ＞T gene distribution in the MS and control group conformed to Hardy-Weinberg equilibrium (x2 = 1.21, P =0.28 ;x2= 1.51 ,P =0.20 respectively). In the MS group, the frequency of C/C, T/C and T/T in the FOXC2-512C ＞ T gene was 26.7%, 58.3% and 15.0% respectively. However, the frequency in the control group was 13.9%, 39.3% and 46.7% respectively( P ＜0.05). The frequency of C and T allele between the MS and control group were significantly different( P ＜0.05 ). Multivariable Logistic regression results showed that FOXC2-512C ＞ T genotype,FBG, TG, BP and BMI were risk factors of MS in the Mongolian. Conclusions The study shows that T allele might be the protective factor of MS while C allele might be the risk factor. FOXC2-512C ＞T genotype is a highly risk factor of MS, which might be the new target of the therapy for MS. However, further larger study is warranted to verify whether FOXC2-512C ＞ T gene can be molecular genetic marker of Inner Mongolia in China.     

N-Acetyltransferase 2 gene polymorphism in a group of senile dementia patients in Shanghai suburb

AIM: To investigate the possible association of hereditary polymorphism of N-acetyltransferase 2 (NAT2) gene with the susceptibility towards senile dementia in farmer population of Shanghai suburb. METHODS: NAT2 gene genotyping was performed at 7 major polymorphic loci (G191A, C282T, T341C, C481T, G590A, A803G, and G857A) with a polymerase chain reaction-based restriction fragment length polymorphism based procedure in 2 groups of farmer subjects in Shanghai suburb. A group of 51 diagnosed dementia patients [comprising 29 sporadic Alzheimer disease (AD) patients and 22 sporadic vascular dementia (VD) patients] and a group of 112 healthy individuals were in the same area. RESULTS: The homogenous rapid genotypes (R/R, including*4/*4, *13 /*13, and *4/*13) was found over-present in both groups of patients, compared with healthy individuals, for all farmer dementia patients, 52.9 % vs 33.0 %, P=0.016, OR (95 % CI): 2.28(1.16-4.48); for AD group only, 51.7 % vs 33.0 %, P=0.063, OR (95 % CI): 2.18 (0.95-4.97);     

Association of apolipoprotein E 4 polymorphism with cerebral infarction in Chinese Han population

AIM: To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS: First-ever cerebral infarction patients (n=226) whose ages ranged from 40 to 60 years old were recruited from Department of Neurology, Zhongshan Hospital, Shanghai, and Zhejiang Chinese Traditional Medicine Hospital, Zhejiang, China. Unrelated healthy controls (n=201) were selected from the general population in the same area with similar age and sex distribution. APOE was amplified by one-stage PCR using the forward primer: 5‘-GGC ACG GCT GTC CAA GGA GCT-3‘ and reverse primer: 5‘-GAT GGC GCT GAG GCC GCG CT-3′. The PCR product was digested directly with 5 U of CfoI and separated by a 20 % polyacryla-mide (acrylamide: bis-acrylamide=29:1) nondenaturing gel. RESULTS: Both cerebral infarction patient and control groups were in Hardy-Weinberg equilibrium. The allele frequency of APOE*2, APOE*3, and APOE*4 was 4.6 %,81.9 %, and 13.5 % respectively in the patients with cerebral infarction; 5.7 %, 87.3 %, and 7.0 % respectively in the healthy control group. Compared with APOE3/3 subjects, APOE4/4 carriers had a 2.1-fold risk of cerebral infarction (odds ratio 2.1, 95 % confidence limits 1.3 to 3.4). The allele frequency of APOE*4 in the cerebral infarction patient group was significantly higher than that in the control group (13.5 % vs 7.0 %; P=0.002).CONCLUSION: APOE 4 is a risk factor for cerebral infarction among the Chinese Han population.     

孕期体质量干预对妊娠结局的影响

Objective To investigate the possibility of gestational body weight intervention and its influence on pregnancy outcomes.Methods 300 healthy primiparae with normal pregestational BMI and living monoeyesis and head presentation were randomly divided into the observation group (n = 120) and control group ( n = 180).Both groups were received general health education and regular obstetric examination, in addition, the observation group received body weight intervention.The data about changes in BMI and body weight from pregestation to ante partum were collected and pregnancy outcomes were assessed.Results The incidence of △ BMI≥6,gestensive hypertension, diabetes, cesarean section,large-for-date infant,intrauterine fetal distress,postparum hemorrhage in the observation group was significantly lower than those of the control group( x2 = 19.265,4.903,6.875,13.522,7.354,4.545,3.993, all P < 0.05).Conclusion Body weight intervention had a preferable feasibility and could improve pregnancy outcome.     

老年2型糖尿病患者血清内生肌酐清除率与代谢综合征的相关性探讨

目的 探讨老年2型糖尿病患者血清内生肌酐清除率(Ccr)与代谢综合征(MS)及其主要组成成分的相关性.方法 选择我院住院的老年2型糖尿病患者329例,根据有无MS分为不伴MS组(144例)和伴MS组(185例),比较2组血清肌酐清除率,分析其与代谢综合征及其主要组成成分的相关性.结果 329例老年2型糖尿病患者中代谢综合征患病率达56.23%(185例);与不伴MS组相比,伴MS组患者BMI、血清TG、收缩压、舒张压明显增高,HDL-C、Ccr[(105.2±28.1)比(93.1±29.1)ml/(min·1.73 m2)]明显降低(均P＜0.05);Ccr与BMI、TG、SBP、DBP及代谢异常集聚数目呈显著负相关;调整了年龄、性别、糖尿病病程、糖化血红蛋白后,Ccr与代谢综合征及其主要组成成分中的TG、B MI、DBP呈显著负相关.结论 老年2型糖尿病患者代谢综合征患病率高,积极多因素干预治疗可能有助于糖尿病肾病的防治.

Abstract：

Objective To investigate the relevance between endogenous creatinine clearance rate(Ccr) and metabolic syndrome and its components in elderly patients with type 2 diabetes. Methods Totally 392 elderly patients with type 2 diabetes were divided into two groups based on with/without metabolic syndrome. Compared the changes of Ccr in the two groups. The relationship between Ccr and metabolic syndrome and its components was analyzed. Results The prevalence of metabolic syndrome in 329 elderly patients with type 2 diabetes was 56.23%;compared to N MS group, the levels of body mass index (BMI), triglyceride (TG), systolic blood pressure(SBP)and diastolic blood pressure(DBP) in MS group increased obviously, but high density lipoprotein-cholesterol(HDLnegative correlation with BMI, TG, SBP, DBP and the number of metabolism mustering(r=-0.190, -0.277, -0.156, - 0.128, -0.214, P ＜ 0.05 ). After adjusting the age, gender, the ascend duration of diabetes and glycosylated hemoglobin A1C, Ccr had a negative correlation with metabolic syndrome and it's components of TG, BMI,DBP( P＜0.05). Conclusion There is high prevalence of metabolic syndrome in elderly patients with type 2 diabetes. It helps to prevent and treat diabetic nephropathy by controlling the levels of the components of metabolic syndrome.     

肾绞痛的社区治疗对策探讨

Object To investigate the effective therapeutic to control nephric colic without anesthetics by comparing the different drugs. Methods 512 patients with moderate or severe nephric colic (the severity of pain was scored by verbal report) were randomized into three groups, including (1) group pethidine +654-2, (2) group lornoxicam + 654-2,and (3) group rotundine +654-2. The effects on alleviation of pain were evaluated among three groups within 40 minutes after drug administration.Results The excellence rate iss 86% and effective rate is 90% in group pethidine + 654-2. The excellence rate are 70% and 65%, respectively,in group lornoxicam + 654-2 and group rotundine +654-2, which are both lower than that in group pethidine +654-2 (both P＜ 0.05), however, the effective rates were 92% and 89%, respectively, which are not significantly different from that in group pethidin + 654-2 (both P＞ 0.05). Comclusion Lornoxicam and rotundine can be widely used to replace pethidine to alleviate nephric colic.     

国产奈达铂联合氟尿嘧啶治疗晚期食管癌疗效观察

目的 观察国产奈达铂+氟尿嘧啶(5-Fu)方案治疗晚期食管癌的近期疗效和不良反应.方法 将55例食管癌患者,完全随机分为治疗组(28例)和对照组(27例).治疗组方案:奈达铂,80～100mg/m2,静脉滴注,d1;5-Fu,750～800 mg/m2,采用便携式泵持续静脉滴注,d1-5.对照组方案:顺铂,50 mg/m2,静脉滴注,d1-3;5-Fu的用法同治疗组,每4周重复1次,共4周期.结果 治疗组和对照组的有效率分别为57.7%(15/26)和36.4%(8/22),差异无统计学意义(P＞0.05).治疗组各度恶心呕吐的发生率均明显低于对照组(均P＜0.05);治疗组Ⅰ～Ⅱ度肾脏损害的发生率低于对照组(P＜0.05).结论 奈达铂+5-Fu治疗晚期食管癌的疗效不低于顺铂+5-Fu,且不良反应易于耐受,值得临床推广.

Abstract：

Objective To observe the short term efficacy and side effects of China-made nedaplatin (NDP)and fluorouracil (5-Fu) treating advanced esophageal carcinoma. Methods The therapeutic regimen for NDP group was NDP plus 5-Fu. The therapeutic regimen for control group was Cisplatin plus 5-Fu. Results The efficiency of NDP group was 57.7% (15/26) and the efficiency of the control group was 36.4% (8/22) (P ＞0.05).The rates of different nausea and vomiting in NDP group were significantly lower than those in control group ( P ＜0.05 ). The rate of renal toxicity low the control group( P ＜0.05 )Diarrhea of two group mostly Appeared Ⅰ-Ⅱ degree; The rate of Ⅰ-Ⅱ degree of leukopenia in the NDP group was higher than that in the control group( P ＞ 0.05 ).The rate of thrombocytopenia in the NDP group was higher than that in the control group( P ＞ 0.05 ). Conclusions The efficacy of NDP group is no lower than the control group. NDP group is easily tolerant to the side effect. It can be used widely in clinic.     

盐酸氟哌噻吨美西辛辅助治疗肠易激综合征66例疗效分析

Objective To investigate the effect of deanxit in adjuvant treatment of the patients with irritable bowel syndrome and the psychologicalon of the patients. Methods Clinical data of 66 patients with irritable bowel syndrome were retrospectively analyzed. The control group was treated by conventional therapy, and the observation group was given deanxit based on the treatment of the control group. Then the curative effect were observed. Results In 132 patients,98 cases were troubed with anxiety,9 cases with athymia, the incidence rate was 81%. After treatment the SAS and SDS score of the observation group reduced,compared with before treatment,and the difference was significant( P ＜0. 05 ); Before and after treatment the SAS and SDS score of the control group had no significant difference(P ＞0. 05). Compared with the control group,the excellence and total effective rate increased significantly in the observation group(P ＞ 0. 05). Conclusion Adjunctive therapy with deanxit could improve the anxiety and athymia,and elevate clinical curative effect.     

Polymorphism of Prodynorphin promoter is associated with schizophrenia in Chinese population

AIM: To investigate the correlation between single nucleotide polymorphisms (SNPs) of functional candidate gene Prodynorphin (PDYN) and schizophrenia. METHODS: SNPs in the promoter and exon regions of PDYN were screened and genotyped for association study in a cohort of Chinese Han schizophrenia cases and controls. RESULTS: Two SNPs PDYN-1576C>T and PDYN-946C>G were identified in the promoter region but PDYN-946C>G showed significant differences of allele distribution (x2=6.15, P=0.013) and genotype distribution (x2=6.87, P=0.032) between schizophrenic and control subjects. CONCLUSION: PDYN-946C>G polymorphism demonstrated an association with population susceptibility to schizophrenia (P<0.05).     

银杏叶提取物对糖尿病大鼠肾脏基质金属蛋白酶2表达的影响

Objective To study the effect of ginkgo biloba extract (GbE) on the expression of matrix metalloproteinase (MMP)-2 in kidneys of diabetic rates and to analyze the protecting mechanism of GbE on diabetic nephropathy. Methods Totally 24 SD rats of diabetic nephropathy induced by streptozotocin were randomly divided into two groups: diabetes group and treatment group with GbE, and 12 rats with placebo therapy were enrolled as the normal control group. Rats were killed after 4 ～ 8 weeks treatment and the expression of MMP-2 and type Ⅳ collagen were studies by immunohistochemistry and RT-PCR. Results In diabetic rats, blood sugar, weight and kidney/body weight ratio were decreased significantly and the excretion of 24 hour urinary protein was increased significantly compared to the diabetes group. But the excretion of 24 hour urinary protein was improved in GbE group ( P ＜0.05 ). The expression level of MMP-2 protein and mRNA was decreased significantly in renal glomduri of diabetic rats ( P ＜ 0.05 ) but showed no changes in GbE treatment group compared to diabetes group( P ＞ 0.05 ). The expression level of type Ⅳ collagen was significantly increased in diabetic rats( P ＜0.05 ) but showed no changes in GbE group( P ＞0.05). Conclusion GbE can improve diabetic nephropathy by inhibiting the expression of MMP-2 in diabetic Rat.     

银杏叶提取物对糖尿病大鼠肾脏基质金属蛋白酶2表达的影响

Objective To study the effect of ginkgo biloba extract (GbE) on the expression of matrix metalloproteinase (MMP)-2 in kidneys of diabetic rates and to analyze the protecting mechanism of GbE on diabetic nephropathy. Methods Totally 24 SD rats of diabetic nephropathy induced by streptozotocin were randomly divided into two groups: diabetes group and treatment group with GbE, and 12 rats with placebo therapy were enrolled as the normal control group. Rats were killed after 4 ～ 8 weeks treatment and the expression of MMP-2 and type Ⅳ collagen were studies by immunohistochemistry and RT-PCR. Results In diabetic rats, blood sugar, weight and kidney/body weight ratio were decreased significantly and the excretion of 24 hour urinary protein was increased significantly compared to the diabetes group. But the excretion of 24 hour urinary protein was improved in GbE group ( P ＜0.05 ). The expression level of MMP-2 protein and mRNA was decreased significantly in renal glomduri of diabetic rats ( P ＜ 0.05 ) but showed no changes in GbE treatment group compared to diabetes group( P ＞ 0.05 ). The expression level of type Ⅳ collagen was significantly increased in diabetic rats( P ＜0.05 ) but showed no changes in GbE group( P ＞0.05). Conclusion GbE can improve diabetic nephropathy by inhibiting the expression of MMP-2 in diabetic Rat.     

急性冠状动脉综合征与高敏C反应蛋白相关性研究

Objective To investigate the relation among triglyce ride (TG), low density lipoprotein cholesterol(LD-C) and high sensitivity C reactive protein(hs-CRP) in acute coronary syndrome(ACS). Methods A total of 132 coronary heart disease patients which were divided as ACS and stable coronary heart dis-ease(SCHD)groups and 65 cases of the health physical exam inees were enrolled as the controls. The levels of hs-CRP, TG,HDL-C and LDL-C among the three groups were analyzed. Results The levels of hs-CRP of patients in ACS and SCHD groups were significantly higher than those in controls group(P ＜0.05). The level of hs-CRP in ACS group was obviously higher than that in SCHD group( P ＜0.01 ). There was a significant correlation between the concentration of hsCRP and TG( r = 0.62 ,P ＜ 0.01 ); there was a significant correlation between hs-CRPand LDL-C ( r =0.41, P ＜ 0.01 ); however there was a significantly negative correlation between hs-CRP and HDL-C ( r = -0.54, P ＜0.01 ). Conclusions The hs-CRP level in patients with acute coronary syndrome is obviously increased. hs-CRP is positively correlated with acute coronary syndrome, hs-CRP is the unstable sign of atheromatous plaque.     

急性冠状动脉综合征与高敏C反应蛋白相关性研究

Objective To investigate the relation among triglyce ride (TG), low density lipoprotein cholesterol(LD-C) and high sensitivity C reactive protein(hs-CRP) in acute coronary syndrome(ACS). Methods A total of 132 coronary heart disease patients which were divided as ACS and stable coronary heart dis-ease(SCHD)groups and 65 cases of the health physical exam inees were enrolled as the controls. The levels of hs-CRP, TG,HDL-C and LDL-C among the three groups were analyzed. Results The levels of hs-CRP of patients in ACS and SCHD groups were significantly higher than those in controls group(P ＜0.05). The level of hs-CRP in ACS group was obviously higher than that in SCHD group( P ＜0.01 ). There was a significant correlation between the concentration of hsCRP and TG( r = 0.62 ,P ＜ 0.01 ); there was a significant correlation between hs-CRPand LDL-C ( r =0.41, P ＜ 0.01 ); however there was a significantly negative correlation between hs-CRP and HDL-C ( r = -0.54, P ＜0.01 ). Conclusions The hs-CRP level in patients with acute coronary syndrome is obviously increased. hs-CRP is positively correlated with acute coronary syndrome, hs-CRP is the unstable sign of atheromatous plaque.     

康妇特栓联合波姆治疗宫颈柱状上皮异位的临床效果观察

Objective To 0bserve the clinical efficacy of KFTS in combination with POME infra-red in the treatment of columnar ectopy.Methods A case control prospective clinical Observe was carried out to explore the effect of KFTS in combination with POME infra-red 75 cases with columnar ectopy as 0bserve group, and interferon in combination with POME infra-red on 75 as control group. Then 0bserve the join of columnar ectopy, the lasting time of the vagina liquor drainage, the percentage of bleeding and adverse reactions after exposure of POME infra-red. Results The heal of the columnar ectopy of the two groups Two months after the operation, the cure rate of the KFTS and POME infra-red group and the control group were respectively 90% (68) and 77.3% (58) while the efficiency were 100% and 90.7%. Obvious significant differences in treat efficiency between agents were observable (P＜0.05). The result after three months: the cure rate of the KFIS and POME infra-red group and the control group were respectively 96% (72) and 84% (63) while the efficiency were 100% and 93.3%. Also there are obvious significant differences in treat efficiency (P＜0.05). The duration of vagina letting of the KFTS and POME infra-red group is shorter than that of the control group and obvious significant differences in treat efficiency between agents were observable (P＜0.05). Also the frequency of bleeding after the operation of the KFTS and POME infra-red group is lower than the control group and obvious significant differences in treat efficiency between agents were observable (P＜0.05).Neither group showed any ill reaction while experiments.Conclusion As the pretreatment medicine in the POME infra-red treatment of columnar ectopy, KRTS can accelerate the heal of the erosion, shorten the duration of vagina letting and debase the frequency of bleeding. Its efficiency shows it is worth promoting.     

Polymorphisms in ghrelin and heparan sulfate proteoglycan genes and their association with diabetic nephropathy in Pakistani population

Diabetic nephropathy(DN),a long term complication of diabetes,is the most common cause of end-stage renal disease,increasing the risk of death.Genetic predispositions play an important role in determining the susceptibility of the development of DN.Heparan sulphate proteoglycan(HSPG) and ghrelin(GH) gene polymorphisms are associated with the risk of DN.T allele frequency of the HSPG gene determined by BamHI polymorphism located in intron 6 may be a risk factor for the development of renal dysfunction in DN(Fisher two tailed test,CI = 95%,d.f.= 29,P = 0.016).The ghrelin gene polymorphism is caused by a cytosine-to-adenine transition in exon 2 of the preproghrelin gene forming Leu72Met variant.In Pakistani population,the preproghrelin Leu72Met polymorphism was observed to be not associated with diabetic nephropathy in patients as indicated by statistical analysis(CI = 95%,d.f.= 29,P = 0.691).The allelic frequencies of HSPG genetic polymorphism has the potential to be used as diagnostic markers for diabetic nephropathy disease.     

C-reactive protein polymorphisms and genetic susceptibility to ischemic stroke and hemorrhagic stroke in the Chinese Han population

Aim： The inflammatory marker C-reactive protein （CRP） has been strongly correlated with the risk of cardiovascular disease. Some single-nucleotide polymorphisms （SNPs） have been reported to be associated with serum CRP levels. In this study, we assessed the genetic association between SNPs within the CRP gene and ischemic and hemorrhagic stroke in the Han Chinese population.
Methods： This study comprises 564 ischemic stroke patients, 220 hemorrhagic stroke patients and 564 controls from the ethnic Han Chinese population in Wuhan. Four CRP SNPs, -757A〉G （rs3093059）, -717A〉G （rs2794521）, -286C〉T〉A （rs3091244） and ＋2147C〉T （rs1205）, were genotyped from patients using TaqMan assays.
Results： The A allele frequency for the -717A〉G polymorphism was significant higher in controls than in ischemic stroke patients （P=0.037）, after adjustment for traditional risk factors （odds ratio 0.28; 95% CI 0.12-0.65; P=0.003）, suggesting a protective effect for this allele against ischemic stroke. Haplotype analysis showed that the H3 （G-C-C） haplotype conferred a significantly increased risk of ischemic stroke （odds ratio 1.052, 95% CI 1.001-1.106： P=0.047）. Neither CRP genotypes nor haplotypes showed an association with hemorrhagic stroke. However, the frequency for haplotype H5 （A-T-C） was significantly higher in ischemic stroke than hemorrhagic stroke patients （P=0.0003）.
Conclusions： These data suggest that the CRP gene -717A allele confers a protective effect against ischemic stroke. Furthermore, the H3 haplotype （G-C-C） is an independent risk marker for ischemic stroke, whereas the H5 haplotype （A-T-C） can be used as a prognostic marker of hemorrhagic stroke.     

高脂血症性急性胰腺炎的临床特点及相关分析

Objective To study the clinical features of hyperlipidemic acute pancreatitis(HL-AP)and to en-hyance the awareness of diagnosis and treatment of HL-AP. Methods To Retrospective analyse 159 cases with acute pancreatitis (AP), of which 18 cases of HL-AP(HL-AP group), 141 cases of other causes of AP(non-HL-AP group),to compare the age, triglyceride (TG), calcium (Ca2+) levels, blood glucose (GLU), CT severity index (CTSI), acute physiology and chronic health evaluation (APACHE Ⅱ) and the the incidence of combined diabetic ketoacidosis (DKA) in two groups,correlative analysis between TG in HL-AP group and the above-mentioned indicators was car-rird out. Results In addition to significantly decreased Ca22+ , TG, APACHEⅡ score, CTSI points, GLU, and the combined incidence of DKA were significantly increased (both P < 0. 05) in HL-AP group when compared with the control group,TG and APACHE Ⅱ score, CTSI score were positively correlated(P < 0. 05),TG and the Ca2+ was negatively correlated (r = - 0. 795, P < 0. 01). Conclusion HL-AP is not uncommon, mainly patients are young and middle-aged with positively correlated between serum TG levels and the HL-AP lesions, and the condition is more seri-ous and,we should pay attention to the early diagnosis and treatment of HL-AP, main treatment is non-surgical treat-ment.     

高压氧对大鼠急性胰腺炎T细胞亚群及电镜超微结构变化的影响

Objective To investigate the effect of hyperbaric oxygen therapy on T-lymphocyte subpopulations in rats with acute pancreatitis. Methods 56 rats were randomly divided into three groups the sham group ( n = 8 ),control group( n = 24) and hyperbaric oxygen therapy group( treatment group, n = 24), then the control group and treatment groups were divided into three subgroups of 8 rats each undergoing euthanasia on days 1,3,7 after the acute pancreatitis induction. The CD4+ ,CD8+ subpopulations of T-lymphocytes in peripheral blood were detected respectively at the fist day and each day of the euthanasia. The sham group was used to make sure that the model was successfully induced. After euthanasia the pancrea was examined using electron microscopy. Results In the control group, the CD4+ cells in AP rats was significantly decreased and the ratio of CD4+/CD8+ also decreased. After 7days of HBO therapy,compared with the control group, the CD4+ lymphocytes of peripheral blood in the treatment group markedly increased( P ＜0.01 ). The CD8+ lymphocytes also increased to a certain extent. And the CD4+/CD8+ ratio of peripheral blood was obviously increased(P ＜0. 01 ). Also more severe pathological changes appeared in the untreated group than in the treatment group. Conclusion Hyperbaric oxygen therapy could improve the oxygen supply in acute panereatitis, regulate T cell immune function.     

RNAi 靶向沉默 CDX2 基因对白血病细胞生物学行为的影响及其作用机制

Abstract: Objective To detect the effects of siRNA targeting CDX2 gene expression on of BCR-ABL, caspase and Bax expressions, and the mechanisms thereof. Methods According to the earlier experiments, siRNA specifically targeting CDX2 gene (CDX2-siRNA) and the negative control sequence (CDX2-siRNA-NC) were selected, and then were transfected into K562 cells by Roche X-tremeGENE HP DNA Transfection Reagent. The flow cytometry analysis was used to detect the effects of siRNA on cell apoptosis. The expressions of BCR-ABL, caspase- 9, Bax mRNA and protein were tested by RTPCR and Western blot assay. Results MTT and flow cytometry analysis showed that after the silence of CDX2 gene expression, the proliferation of K562 cells was prohibited and the apoptotic rate of K562 cells was distinctly increased compared with that of normal cell group, but the negative control group had no significant change. According to the RT-PCR and Western blot assay, in comparison with the normal cell group and the negative control group, the expression levels of BCR-ABL mRNA and protein were obviously decreased, and the difference was statistic significance. On the other hand, the expressions of caspase- 9 and Bax mRNA and protein were significantly higher than those of other two groups (P＜0.05).Conclusion CDX2-siRNA can promote apoptosis of K562 cells obviously, and the mechanism is related with the downregulation of BCR-ABL and the up-regulation of caspase-9 and Bax.